(Circular) (Six-base) MAPSORT of: pFN21AB6327.seq Check: 4301 from: 1 to: 5488 pFN21AB6327 5488 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2622 2809 2622 Size: 187 5301 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3551 4935 5203 1632 Size: 1919 1384 268 1917 Fragments arranged by size: 1919 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 4387 5364 1203 Size: 3184 977 1327 Fragments arranged by size: 3184 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 2195 1166 Size: 1029 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 3231 4803 3231 Size: 1572 3916 ApaI G_GGCC'C Cuts at: 2402 2402 Size: 5488 ApaLI G'TGCA_C Cuts at: 3545 4701 3545 Size: 1156 4332 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2813 1870 Size: 73 375 495 4545 Fragments arranged by size: 4545 495 375 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2622 2809 4264 618 Size: 325 217 627 835 187 1455 1842 Fragments arranged by size: 1842 1455 835 627 325 217 187 BanII G_rGCy'C Cuts at: 1391 2244 2325 2402 2807 1391 Size: 853 81 77 405 4072 Fragments arranged by size: 4072 853 405 81 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 928 Size: 971 446 4071 Fragments arranged by size: 4071 971 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2052 3380 4590 2052 Size: 1328 1210 2950 Fragments arranged by size: 2950 1328 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2397 4099 136 Size: 107 122 71 631 605 725 1702 1525 Fragments arranged by size: 1702 1525 725 631 605 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2402 2509 3549 4705 1790 Size: 612 107 1040 1156 2573 Fragments arranged by size: 2573 1156 1040 612 107 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 2573 3219 Size: 416 53 83 399 24 1122 34 646 Cuts at: 3219 4177 442 Size: 958 1753 Fragments arranged by size: 1753 1122 958 646 416 399 83 53 34 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2309 2492 4148 1239 Size: 989 81 183 1656 2579 Fragments arranged by size: 2579 1656 989 183 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 3613 4478 4776 2210 Size: 1403 865 298 2922 Fragments arranged by size: 2922 1403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 2671 4151 882 Size: 617 597 575 1480 2219 Fragments arranged by size: 2219 1480 617 597 575 BsaAI yAC'GTr Cuts at: 493 1204 2776 3279 493 Size: 711 1572 503 2702 Fragments arranged by size: 2702 1572 711 503 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 3677 4312 275 Size: 53 83 186 936 1762 382 635 1451 Fragments arranged by size: 1762 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3921 4319 4593 4740 5151 3921 Size: 398 274 147 411 4258 Fragments arranged by size: 4258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2651 2681 1178 Size: 30 165 30 1248 30 3985 Fragments arranged by size: 3985 1248 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2390 1186 Size: 1096 108 4284 Fragments arranged by size: 4284 1096 108 BseYI C'CCAG_C Cuts at: 2526 4691 5187 2526 Size: 2165 496 2827 Fragments arranged by size: 2827 2165 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2720 1163 Size: 573 984 3931 Fragments arranged by size: 3931 984 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3699 4727 1540 Size: 275 186 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2325 3549 3634 4705 2244 Size: 81 1224 85 1071 3027 Fragments arranged by size: 3027 1224 1071 85 81 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2325 2402 2509 2807 3549 3634 Size: 399 454 81 77 107 298 742 85 Cuts at: 3634 4705 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 298 107 85 81 77 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2114 2843 844 Size: 1270 729 3489 Fragments arranged by size: 3489 1270 729 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 3983 4157 65 Size: 1183 847 1888 174 1396 Fragments arranged by size: 1888 1396 1183 847 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2460 2536 2744 2764 4132 4319 1700 Size: 110 650 76 208 20 1368 187 2869 Fragments arranged by size: 2869 1368 650 208 187 110 76 20 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2776 2552 Size: 224 5264 BstYI r'GATC_y Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483 Size: 846 367 385 17 1452 86 12 346 Cuts at: 5483 1972 Size: 1977 Fragments arranged by size: 1977 1452 846 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2046 2193 513 Size: 552 981 147 3808 Fragments arranged by size: 3808 981 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3798 3818 3004 Size: 794 20 4674 Fragments arranged by size: 4674 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2690 4495 809 Size: 507 1374 1805 1802 Fragments arranged by size: 1805 1802 1374 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2766 2935 3824 Size: 54 1425 50 275 18 936 169 889 Cuts at: 3824 5453 8 Size: 1629 43 Fragments arranged by size: 1629 1425 936 889 275 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 3418 1353 Size: 1028 1037 3423 Fragments arranged by size: 3423 1037 1028 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 4075 4449 4595 387 Size: 1064 138 627 1859 374 146 1280 Fragments arranged by size: 1859 1280 1064 627 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2323 2242 Size: 81 5407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2309 2492 3551 4148 4935 5203 Size: 393 596 81 183 1059 597 787 268 Cuts at: 5203 1239 Size: 1524 Fragments arranged by size: 1524 1059 787 597 596 393 268 183 81 EcoO109I rG'GnC_Cy Cuts at: 2399 2399 Size: 5488 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 5488 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 2601 2649 4635 1678 Size: 923 48 1986 2531 Fragments arranged by size: 2531 1986 923 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2651 2683 4129 Size: 32 135 32 454 32 593 32 1446 Cuts at: 4129 4161 4203 4235 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 593 454 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2695 2832 3078 669 Size: 988 281 757 137 246 3079 Fragments arranged by size: 3079 988 757 281 246 137 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2626 2813 2626 Size: 187 5301 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3666 Fragments arranged by size: 3666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2756 3446 3805 3964 518 Size: 648 1029 561 690 359 159 2042 Fragments arranged by size: 2042 1029 690 648 561 359 159 MspA1I CmG'CkG Cuts at: 1606 2904 3581 4729 4974 1606 Size: 1298 677 1148 245 2120 Fragments arranged by size: 2120 1298 1148 677 245 NaeI GCC'GGC Cuts at: 2766 2766 Size: 5488 NcoI C'CATG_G Cuts at: 513 1065 2193 513 Size: 552 1128 3808 Fragments arranged by size: 3808 1128 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NgoMIV G'CCGG_C Cuts at: 2764 2764 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2091 2846 4391 1132 Size: 97 862 755 1545 2229 Fragments arranged by size: 2229 1545 862 755 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 2609 1108 Size: 1501 3987 PfoI T'CCnGG_A Cuts at: 2673 2673 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PspOMI G'GGCC_C Cuts at: 2398 2398 Size: 5488 PstI C_TGCA'G Cuts at: 830 2575 2840 830 Size: 1745 265 3478 Fragments arranged by size: 3478 1745 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SacI G_AGCT'C Cuts at: 2244 2325 2244 Size: 81 5407 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SapI GCTCTTCn'nnn_ Cuts at: 2381 2381 Size: 5488 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2571 2641 2836 3971 4652 4843 826 Size: 219 1526 70 195 1135 681 191 1471 Fragments arranged by size: 1526 1471 1135 681 219 195 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 2744 1700 Size: 1044 4444 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 3592 4493 4755 820 Size: 197 926 282 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 282 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2091 2846 1132 Size: 959 755 3774 Fragments arranged by size: 3774 959 755 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5488 StyI C'CwwG_G Cuts at: 513 1065 2193 2927 513 Size: 552 1128 734 3074 Fragments arranged by size: 3074 1128 734 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3515 3532 3685 3870 4315 325 Size: 1255 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3734 5446 96 Size: 274 80 33 51 1443 1757 1712 138 Fragments arranged by size: 1757 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5448 5480 5448 Size: 32 5456 Tth111I GACn'n_nGTC Cuts at: 1316 2663 1316 Size: 1347 4141 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2552 2552 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 2217 3617 2217 Size: 1400 4088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI