(Circular) (Six-base) MAPSORT of: pFN21AB6133.seq Check: 1013 from: 1 to: 6475 pFN21AB6133 6475 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 3796 3796 Size: 6475 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2236 2849 3646 4538 5922 6190 1632 Size: 406 198 613 797 892 1384 268 1917 Fragments arranged by size: 1917 1384 892 797 613 406 268 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 5374 6351 1203 Size: 4171 977 1327 Fragments arranged by size: 4171 1327 977 AgeI A'CCGG_T Cuts at: 2259 5306 2259 Size: 3047 3428 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 2105 2969 1166 Size: 939 864 4672 Fragments arranged by size: 4672 939 864 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6475 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 2642 Size: 32 6443 AlwNI CAG_nnn'CTG Cuts at: 2647 2752 3190 4218 5790 2647 Size: 105 438 1028 1572 3332 Fragments arranged by size: 3332 1572 1028 438 105 ApaLI G'TGCA_C Cuts at: 4532 5688 4532 Size: 1156 5319 ApoI r'AATT_y Cuts at: 1493 3731 3784 4010 4162 6271 1493 Size: 2238 53 226 152 2109 1697 Fragments arranged by size: 2238 2109 1697 226 152 53 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 3800 1870 Size: 73 1857 4545 Fragments arranged by size: 4545 1857 73 AvrII C'CTAG_G Cuts at: 3628 3628 Size: 6475 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2782 2815 2782 Size: 33 6442 BamHI G'GATC_C Cuts at: 3805 4172 3805 Size: 367 6108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2624 3796 5251 618 Size: 325 217 627 516 321 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 516 325 321 217 BanII G_rGCy'C Cuts at: 1391 2216 2355 3372 3642 3656 3794 1391 Size: 825 139 1017 270 14 138 4072 Fragments arranged by size: 4072 1017 825 270 139 138 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 928 Size: 971 867 4637 Fragments arranged by size: 4637 971 867 BbvCI CC'TCA_GC Cuts at: 3024 3024 Size: 6475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2432 4367 5577 2432 Size: 1935 1210 3330 Fragments arranged by size: 3330 1935 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3129 5086 136 Size: 107 122 71 631 605 1457 1957 1525 Fragments arranged by size: 1957 1525 1457 631 605 122 107 71 BglII A'GATC_T Cuts at: 3159 6470 3159 Size: 3311 3164 BlpI GC'TnA_GC Cuts at: 3892 3892 Size: 6475 Bme1580I G_kGCm'C Cuts at: 1790 2627 4536 5692 1790 Size: 837 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 837 BmgBI CAC'GTC Cuts at: 1318 3397 1318 Size: 2079 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3031 4206 5164 Size: 416 53 83 399 24 1614 1175 958 Cuts at: 5164 442 Size: 1753 Fragments arranged by size: 1753 1614 1175 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2436 2591 2669 2891 3329 3446 3589 Size: 1059 138 155 78 222 438 117 143 Cuts at: 3589 3706 5135 1239 Size: 117 1429 2579 Fragments arranged by size: 2579 1429 1059 438 222 155 143 138 117 117 78 Bpu10I CC'TnA_GC Cuts at: 3024 3024 Size: 6475 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3263 4600 5465 5763 3263 Size: 1337 865 298 3975 Fragments arranged by size: 3975 1337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5138 882 Size: 617 3639 2219 Fragments arranged by size: 3639 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4266 493 Size: 711 3062 2702 Fragments arranged by size: 3062 2702 711 BsaBI GATnn'nnATC Cuts at: 2228 6469 2228 Size: 4241 2234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4282 4664 5299 275 Size: 53 83 186 936 2749 382 635 1451 Fragments arranged by size: 2749 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 2259 3174 4908 5306 5580 5727 6138 2031 Size: 228 915 1734 398 274 147 411 2368 Fragments arranged by size: 2368 1734 915 411 398 274 228 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 3444 3474 1178 Size: 30 165 30 1239 30 772 30 4179 Fragments arranged by size: 4179 1239 772 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 3059 3116 3326 3739 3742 1186 Size: 1333 540 57 210 413 3 3919 Fragments arranged by size: 3919 1333 540 413 210 57 3 BseYI C'CCAG_C Cuts at: 2480 3685 5678 6174 2480 Size: 1205 1993 496 2781 Fragments arranged by size: 2781 1993 1205 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5902 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3925 4686 5714 1540 Size: 275 186 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3372 4536 4621 5692 3372 Size: 1164 85 1071 4155 Fragments arranged by size: 4155 1164 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3986 4079 3443 Size: 543 93 5839 Fragments arranged by size: 5839 543 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2355 2627 3372 3642 3656 3794 Size: 399 426 139 272 745 270 14 138 Cuts at: 3794 4536 4621 5692 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 426 399 272 270 139 138 85 14 BspEI T'CCGG_A Cuts at: 2031 6138 2031 Size: 4107 2368 BspHI T'CATG_A Cuts at: 2910 3372 4364 6094 2910 Size: 462 992 1730 3291 Fragments arranged by size: 3291 1730 992 462 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 3830 844 Size: 1631 1355 3489 Fragments arranged by size: 3489 1631 1355 BsrBI CCG'CTC Cuts at: 1394 1823 2037 4362 1394 Size: 429 214 2325 3507 Fragments arranged by size: 3507 2325 429 214 BsrDI GCAATG_nn' Cuts at: 65 1248 3243 4970 5144 65 Size: 1183 1995 1727 174 1396 Fragments arranged by size: 1995 1727 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2306 3123 5119 5306 1700 Size: 110 449 47 817 1996 187 2869 Fragments arranged by size: 2869 1996 817 449 187 110 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6475 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6475 BstBI TT'CG_AA Cuts at: 5279 5279 Size: 6475 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstYI r'GATC_y Cuts at: 1972 3159 3805 4172 4557 4574 6026 6112 6124 Size: 1187 646 367 385 17 1452 86 12 Cuts at: 6124 6470 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1187 646 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2681 2681 Size: 6475 BtgI C'CryG_G Cuts at: 513 1065 2982 513 Size: 552 1917 4006 Fragments arranged by size: 4006 1917 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3004 3147 487 Size: 683 56 1778 143 3815 Fragments arranged by size: 3815 1778 683 143 56 BtsI GCAGTG_nn' Cuts at: 2802 3499 3520 3526 3595 3619 3679 3709 3991 Size: 697 21 6 69 24 60 30 282 Cuts at: 3991 4785 4805 2802 Size: 794 20 4472 Fragments arranged by size: 4472 794 697 282 69 60 30 24 21 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DraIII CAC_nnn'GTG Cuts at: 2601 2601 Size: 6475 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3121 3300 3676 Size: 54 1425 50 275 18 1291 179 376 Cuts at: 3676 3922 4811 6440 8 Size: 246 889 1629 43 Fragments arranged by size: 1629 1425 1291 889 376 275 246 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3922 1537 Size: 275 2110 4090 Fragments arranged by size: 4090 2110 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3755 4405 1353 Size: 2402 650 3423 Fragments arranged by size: 3423 2402 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2376 5062 5436 5582 387 Size: 1064 138 787 2686 374 146 1280 Fragments arranged by size: 2686 1280 1064 787 374 146 138 EcoICRI GAG'CTC Cuts at: 3370 3370 Size: 6475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2236 2298 2436 2591 2669 2849 Size: 393 406 198 62 138 155 78 180 Cuts at: 2849 2891 3329 3446 3589 3646 3706 4538 5135 Size: 42 438 117 143 57 60 832 597 Cuts at: 5135 5922 6190 1239 Size: 787 268 1524 Fragments arranged by size: 1524 832 787 597 438 406 393 268 198 180 155 143 138 117 78 62 60 57 42 EcoNI CCTnn'n_nnAGG Cuts at: 2415 2612 3323 2415 Size: 197 711 5567 Fragments arranged by size: 5567 711 197 EcoO109I rG'GnC_Cy Cuts at: 2749 3316 2749 Size: 567 5908 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 EcoRV GAT'ATC Cuts at: 2226 2226 Size: 6475 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2938 2938 Size: 6475 FspI TGC'GCA Cuts at: 4981 4981 Size: 6475 HaeII r_GCGC'y Cuts at: 1678 5622 1678 Size: 3944 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373 Size: 32 135 32 3544 32 42 32 2626 Fragments arranged by size: 3544 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2440 3472 3819 4065 669 Size: 988 281 502 1032 347 246 3079 Fragments arranged by size: 3079 1032 988 502 347 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6475 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KpnI G_GTAC'C Cuts at: 3800 3800 Size: 6475 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 6351 6351 Size: 6475 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3442 5589 5773 1305 Size: 2137 2147 184 2007 Fragments arranged by size: 2147 2137 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4653 Fragments arranged by size: 4653 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2105 2915 2969 3132 4433 4792 4951 Size: 648 939 810 54 163 1301 359 159 Cuts at: 4951 518 Size: 2042 Fragments arranged by size: 2042 1301 939 810 648 359 163 159 54 MspA1I CmG'CkG Cuts at: 1606 2692 3119 3891 4568 5716 5961 1606 Size: 1086 427 772 677 1148 245 2120 Fragments arranged by size: 2120 1148 1086 772 677 427 245 NaeI GCC'GGC Cuts at: 3125 3125 Size: 6475 NcoI C'CATG_G Cuts at: 513 1065 2982 513 Size: 552 1917 4006 Fragments arranged by size: 4006 1917 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NgoMIV G'CCGG_C Cuts at: 3123 3123 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2746 2957 3833 5378 1132 Size: 97 1517 211 876 1545 2229 Fragments arranged by size: 2229 1545 1517 876 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6475 PciI A'CATG_T Cuts at: 5374 5374 Size: 6475 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6475 PfoI T'CCnGG_A Cuts at: 2413 2568 3755 2413 Size: 155 1187 5133 Fragments arranged by size: 5133 1187 155 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2642 2674 4544 4576 6057 6089 2642 Size: 32 1870 32 1481 32 3028 Fragments arranged by size: 3028 1870 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 6475 PshAI GACnn'nnGTC Cuts at: 1613 2954 1613 Size: 1341 5134 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 2616 3111 3237 3324 3827 830 Size: 1786 495 126 87 503 3478 Fragments arranged by size: 3478 1786 503 495 126 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2692 3119 2692 Size: 427 6048 RsrII CG'GwC_CG Cuts at: 2115 2115 Size: 6475 SacI G_AGCT'C Cuts at: 3372 3372 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SapI GCTCTTCn'nnn_ Cuts at: 3755 3755 Size: 6475 SbfI CC_TGCA'GG Cuts at: 3827 3827 Size: 6475 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SexAI A'CCwGG_T Cuts at: 2751 2751 Size: 6475 SfcI C'TryA_G Cuts at: 826 1045 2612 3107 3233 3320 3823 4958 5639 Size: 219 1567 495 126 87 503 1135 681 Cuts at: 5639 5830 826 Size: 191 1471 Fragments arranged by size: 1567 1471 1135 681 503 495 219 191 126 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6475 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3278 4579 5480 5742 820 Size: 197 926 1335 1301 901 262 1553 Fragments arranged by size: 1553 1335 1301 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4266 493 Size: 3773 2702 SpeI A'CTAG_T Cuts at: 152 2237 152 Size: 2085 4390 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6475 StyI C'CwwG_G Cuts at: 513 1065 2877 2982 3628 3914 513 Size: 552 1812 105 646 286 3074 Fragments arranged by size: 3074 1812 646 552 286 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 2516 4502 4519 4672 4857 5302 Size: 1255 585 351 1986 17 153 185 445 Cuts at: 5302 325 Size: 1498 Fragments arranged by size: 1986 1498 1255 585 445 351 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 4721 6433 Size: 274 80 33 51 1443 690 2054 1712 Cuts at: 6433 96 Size: 138 Fragments arranged by size: 2054 1712 1443 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2585 2617 6435 6467 2585 Size: 32 3818 32 2593 Fragments arranged by size: 3818 2593 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3209 1316 Size: 1893 4582 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3295 3337 3388 3295 Size: 42 51 6382 Fragments arranged by size: 6382 51 42 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6475 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 2918 3764 4604 2918 Size: 846 840 4789 Fragments arranged by size: 4789 846 840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII SanDI SfiI SfoI SrfI SwaI