(Circular) (Six-base) MAPSORT of: pFN21AA0449.seq Check: 1999 from: 1 to: 9577 pFN21AA0449 9577 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5648 7387 278 Size: 53 83 186 936 4112 1739 2468 Fragments arranged by size: 4112 2468 1739 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6920 1937 Size: 4983 4594 Acc65I G'GTAC_C Cuts at: 6898 6898 Size: 9577 AclI AA'CG_TT Cuts at: 1563 7704 8077 1563 Size: 6141 373 3063 Fragments arranged by size: 6141 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 2864 4259 4711 5109 5277 7640 9024 Size: 1004 228 1395 452 398 168 2363 1384 Cuts at: 9024 9292 1632 Size: 268 1917 Fragments arranged by size: 2363 1917 1395 1384 1004 452 398 268 228 168 AfeI AGC'GCT Cuts at: 5799 5799 Size: 9577 AflII C'TTAA_G Cuts at: 820 1017 4208 820 Size: 197 3191 6189 Fragments arranged by size: 6189 3191 197 AflIII A'CryG_T Cuts at: 1203 8476 9453 1203 Size: 7273 977 1327 Fragments arranged by size: 7273 1327 977 AgeI A'CCGG_T Cuts at: 8408 8408 Size: 9577 AhdI GACnn_n'nnGTC Cuts at: 8306 8306 Size: 9577 AleI CACnn'nnGTG Cuts at: 1166 2204 5012 5060 5702 5860 1166 Size: 1038 2808 48 642 158 4883 Fragments arranged by size: 4883 2808 1038 642 158 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3177 1184 Size: 1993 7584 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4838 4870 4838 Size: 32 9545 AlwNI CAG_nnn'CTG Cuts at: 2125 2446 2614 3646 3949 4174 4759 4936 5076 Size: 321 168 1032 303 225 585 177 140 Cuts at: 5076 6654 6783 7320 8892 2125 Size: 1578 129 537 1572 2810 Fragments arranged by size: 2810 1578 1572 1032 585 537 321 303 225 177 168 140 129 ApaI G_GGCC'C Cuts at: 2629 3106 3304 4594 5468 2629 Size: 477 198 1290 874 6738 Fragments arranged by size: 6738 1290 874 477 198 ApaLI G'TGCA_C Cuts at: 2719 2957 3162 5841 7634 8790 2719 Size: 238 205 2679 1793 1156 3506 Fragments arranged by size: 3506 2679 1793 1156 238 205 ApoI r'AATT_y Cuts at: 1493 6886 7112 7264 9373 1493 Size: 5393 226 152 2109 1697 Fragments arranged by size: 5393 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8131 160 Size: 872 7099 1606 Fragments arranged by size: 7099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9577 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2344 2779 3692 3838 3878 4910 Size: 73 164 237 435 913 146 40 1032 Cuts at: 4910 5267 5604 5631 6043 6130 6256 6528 6628 Size: 357 337 27 412 87 126 272 100 Cuts at: 6628 6902 1870 Size: 274 4545 Fragments arranged by size: 4545 1032 913 435 412 357 337 274 272 237 164 146 126 100 87 73 40 27 AvrII C'CTAG_G Cuts at: 5621 5621 Size: 9577 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2077 2110 2077 Size: 33 9544 BamHI G'GATC_C Cuts at: 2046 6907 7274 2046 Size: 4861 367 4349 Fragments arranged by size: 4861 4349 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4473 4676 6236 6437 6898 Size: 325 217 627 2686 203 1560 201 461 Cuts at: 6898 8353 618 Size: 1455 1842 Fragments arranged by size: 2686 1842 1560 1455 627 461 325 217 203 201 BanII G_rGCy'C Cuts at: 1391 2118 2454 2629 2672 3050 3106 3304 3545 Size: 727 336 175 43 378 56 198 241 Cuts at: 3545 3692 4405 4594 4910 5421 5468 6468 6573 Size: 147 713 189 316 511 47 1000 105 Cuts at: 6573 6896 1391 Size: 323 4072 Fragments arranged by size: 4072 1000 727 713 511 378 336 323 316 241 198 189 175 147 105 56 47 43 BbeI G_GCGC'C Cuts at: 4477 4477 Size: 9577 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2155 2288 4454 5752 928 Size: 971 256 133 2166 1298 4753 Fragments arranged by size: 4753 2166 1298 971 256 133 BbvCI CC'TCA_GC Cuts at: 3246 4079 4548 4941 6224 3246 Size: 833 469 393 1283 6599 Fragments arranged by size: 6599 1283 833 469 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2200 2234 7768 7802 1903 Size: 34 263 34 5534 34 3678 Fragments arranged by size: 5534 3678 263 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7469 8679 7469 Size: 1210 8367 BclI T'GATC_A Cuts at: 1548 2922 1548 Size: 1374 8203 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3101 3575 4259 Size: 107 122 71 631 605 1429 474 684 Cuts at: 4259 4283 6234 6324 8188 136 Size: 24 1951 90 1864 1525 Fragments arranged by size: 1951 1864 1525 1429 684 631 605 474 122 107 90 71 24 BglII A'GATC_T Cuts at: 2190 5331 9572 2190 Size: 3141 4241 2195 Fragments arranged by size: 4241 3141 2195 BlpI GC'TnA_GC Cuts at: 6284 6647 6994 6284 Size: 363 347 8867 Fragments arranged by size: 8867 363 347 Bme1580I G_kGCm'C Cuts at: 1790 2300 2398 2629 2723 2961 3106 3166 3304 Size: 510 98 231 94 238 145 60 138 Cuts at: 3304 4594 5468 5845 6239 6440 7638 8794 1790 Size: 1290 874 377 394 201 1198 1156 2573 Fragments arranged by size: 2573 1290 1198 1156 874 510 394 377 238 231 201 145 138 98 94 60 BmgBI CAC'GTC Cuts at: 1318 6126 1318 Size: 4808 4769 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 2763 5356 Size: 416 53 83 399 24 1017 329 2593 Cuts at: 5356 6613 7308 8266 442 Size: 1257 695 958 1753 Fragments arranged by size: 2593 1753 1257 1017 958 695 416 399 329 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3398 1056 Size: 2342 7235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2902 3631 5062 5350 2902 Size: 729 1431 288 7129 Fragments arranged by size: 7129 1431 729 288 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2981 3549 3668 3983 4073 4427 4588 Size: 1229 513 568 119 315 90 354 161 Cuts at: 4588 4781 4832 5042 6760 8237 1239 Size: 193 51 210 1718 1477 2579 Fragments arranged by size: 2579 1718 1477 1229 568 513 354 315 210 193 161 119 90 51 Bpu10I CC'TnA_GC Cuts at: 3246 4079 4548 4941 6192 6224 3246 Size: 833 469 393 1251 32 6599 Fragments arranged by size: 6599 1251 833 469 393 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3977 4349 4730 5102 7702 8567 8865 3977 Size: 372 381 372 2600 865 298 4689 Fragments arranged by size: 4689 2600 865 381 372 372 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3065 4328 5021 5550 5584 6025 8240 Size: 617 1566 1263 693 529 34 441 2215 Cuts at: 8240 882 Size: 2219 Fragments arranged by size: 2219 2215 1566 1263 693 617 529 441 34 BsaAI yAC'GTr Cuts at: 493 1204 7368 493 Size: 711 6164 2702 Fragments arranged by size: 6164 2702 711 BsaBI GATnn'nnATC Cuts at: 3935 9571 3935 Size: 5636 3941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2568 4474 5238 5645 Size: 53 83 186 936 1035 1906 764 407 Cuts at: 5645 7384 7766 8401 275 Size: 1739 382 635 1451 Fragments arranged by size: 1906 1739 1451 1035 936 764 635 407 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8010 8408 8682 8829 9240 8010 Size: 398 274 147 411 8347 Fragments arranged by size: 8347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2960 2990 4838 4868 6005 Size: 30 165 30 1557 30 1848 30 1137 Cuts at: 6005 6035 1178 Size: 30 4720 Fragments arranged by size: 4720 1848 1557 1137 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2342 2477 2612 2984 3476 3758 3782 Size: 1153 3 135 135 372 492 282 24 Cuts at: 3782 3785 3788 3818 3821 3896 4118 4322 4456 Size: 3 3 30 3 75 222 204 134 Cuts at: 4456 4862 4958 5039 5051 5203 5342 5844 1186 Size: 406 96 81 12 152 139 502 4919 Fragments arranged by size: 4919 1153 502 492 406 372 282 222 204 152 139 135 135 134 96 81 75 30 24 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 2055 2128 2724 3007 3363 6418 8780 9276 2055 Size: 73 596 283 356 3055 2362 496 2356 Fragments arranged by size: 3055 2362 2356 596 496 356 283 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 4449 4533 5036 5315 6730 1163 Size: 573 948 1765 84 503 279 1415 4010 Fragments arranged by size: 4010 1765 1415 948 573 503 279 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4063 5677 7027 7788 8816 1540 Size: 275 186 2062 1614 1350 761 1028 2301 Fragments arranged by size: 2301 2062 1614 1350 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2454 2672 2723 2961 3166 4197 4405 5845 6193 Size: 218 51 238 205 1031 208 1440 348 Cuts at: 6193 6573 7638 7723 8794 2454 Size: 380 1065 85 1071 3237 Fragments arranged by size: 3237 1440 1071 1065 1031 380 348 238 218 208 205 85 51 BsmI GAATG_Cn' Cuts at: 3280 6111 7088 7181 3280 Size: 2831 977 93 5676 Fragments arranged by size: 5676 2831 977 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3740 3780 5636 5774 3740 Size: 40 1856 138 7543 Fragments arranged by size: 7543 1856 138 40 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2300 2398 2454 2629 2672 2723 Size: 399 328 182 98 56 175 43 51 Cuts at: 2723 2961 3050 3106 3166 3304 3545 3692 4197 Size: 238 89 56 60 138 241 147 505 Cuts at: 4197 4405 4594 4910 5421 5468 5845 6193 6239 Size: 208 189 316 511 47 377 348 46 Cuts at: 6239 6440 6468 6573 6896 7638 7723 8794 1391 Size: 201 28 105 323 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 511 505 399 377 348 328 323 316 241 238 208 201 189 182 175 147 138 105 98 89 85 60 56 56 51 47 46 43 28 BspEI T'CCGG_A Cuts at: 9240 9240 Size: 9577 BspHI T'CATG_A Cuts at: 3030 3369 7466 9196 3030 Size: 339 4097 1730 3411 Fragments arranged by size: 4097 3411 1730 339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2505 3396 4173 6279 6932 844 Size: 1661 891 777 2106 653 3489 Fragments arranged by size: 3489 2106 1661 891 777 653 BsrBI CCG'CTC Cuts at: 1394 1823 2839 2869 3652 4498 4596 4828 4891 Size: 429 1016 30 783 846 98 232 63 Cuts at: 4891 7464 1394 Size: 2573 3507 Fragments arranged by size: 3507 2573 1016 846 783 429 232 98 63 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2373 2923 3421 5752 5761 6268 8072 Size: 1183 1125 550 498 2331 9 507 1804 Cuts at: 8072 8246 65 Size: 174 1396 Fragments arranged by size: 2331 1804 1396 1183 1125 550 507 498 174 9 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2828 3404 8221 8408 1700 Size: 110 199 819 576 4817 187 2869 Fragments arranged by size: 4817 2869 819 576 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9577 BssSI C'ACGA_G Cuts at: 2534 6374 7637 8649 2534 Size: 3840 1263 1012 3462 Fragments arranged by size: 3840 3462 1263 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4174 4511 794 Size: 3380 337 5860 Fragments arranged by size: 5860 3380 337 BstBI TT'CG_AA Cuts at: 8381 8381 Size: 9577 BstEII G'GTnAC_C Cuts at: 1184 6346 1184 Size: 5162 4415 BstXI CCAn_nnnn'nTGG Cuts at: 2254 3604 5506 5704 6016 2254 Size: 1350 1902 198 312 5815 Fragments arranged by size: 5815 1902 1350 312 198 BstYI r'GATC_y Cuts at: 1972 2046 2190 2466 2529 4035 4395 5166 5331 Size: 74 144 276 63 1506 360 771 165 Cuts at: 5331 6907 7274 7659 7676 9128 9214 9226 9572 Size: 1576 367 385 17 1452 86 12 346 Cuts at: 9572 1972 Size: 1977 Fragments arranged by size: 1977 1576 1506 1452 771 385 367 360 346 276 165 144 86 74 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2932 4439 2932 Size: 1507 8070 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2292 3603 4086 5061 5871 Size: 552 939 243 45 1311 483 975 810 Cuts at: 5871 513 Size: 4219 Fragments arranged by size: 4219 1311 975 939 810 552 483 243 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2338 3423 487 Size: 683 56 1112 1085 6641 Fragments arranged by size: 6641 1112 1085 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3164 3850 4871 5855 5867 7093 7887 7907 Size: 365 686 1021 984 12 1226 794 20 Cuts at: 7907 2799 Size: 4469 Fragments arranged by size: 4469 1226 1021 984 794 686 365 20 12 ClaI AT'CG_AT Cuts at: 1554 3339 1554 Size: 1785 7792 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9542 DraI TTT'AAA Cuts at: 6881 6881 Size: 9577 DraIII CAC_nnn'GTG Cuts at: 2818 2818 Size: 9577 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2027 5756 6124 8584 809 Size: 507 711 3729 368 2460 1802 Fragments arranged by size: 3729 2460 1802 711 507 368 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2011 2037 Size: 54 1425 50 275 18 177 4 26 Cuts at: 2037 2826 3912 4479 4557 5874 6316 6357 6846 Size: 789 1086 567 78 1317 442 41 489 Cuts at: 6846 7024 7913 9542 8 Size: 178 889 1629 43 Fragments arranged by size: 1629 1425 1317 1086 889 789 567 489 442 275 178 177 78 54 50 43 41 26 18 4 EagI C'GGCC_G Cuts at: 1537 1812 7024 1537 Size: 275 5212 4090 Fragments arranged by size: 5212 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3680 3774 3843 4133 4911 7507 1353 Size: 2327 94 69 290 778 2596 3423 Fragments arranged by size: 3423 2596 2327 778 290 94 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3779 3920 8164 8538 8684 387 Size: 1064 138 2190 141 4244 374 146 1280 Fragments arranged by size: 4244 2190 1280 1064 374 146 141 138 EcoICRI GAG'CTC Cuts at: 2452 2670 4403 6571 2452 Size: 218 1733 2168 5458 Fragments arranged by size: 5458 2168 1733 218 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2636 2864 2981 3549 3668 3983 Size: 393 836 168 228 117 568 119 315 Cuts at: 3983 4073 4259 4427 4588 4711 4781 4832 5042 Size: 90 186 168 161 123 70 51 210 Cuts at: 5042 5109 5277 6760 7640 8237 9024 9292 1239 Size: 67 168 1483 880 597 787 268 1524 Fragments arranged by size: 1524 1483 880 836 787 597 568 393 315 268 228 210 186 168 168 168 161 123 119 117 90 70 67 51 EcoNI CCTnn'n_nnAGG Cuts at: 2618 3535 4862 4865 2618 Size: 917 1327 3 7330 Fragments arranged by size: 7330 1327 917 3 EcoO109I rG'GnC_Cy Cuts at: 2122 2625 2626 3300 3301 3643 4162 4590 5278 Size: 503 1 674 1 342 519 428 688 Cuts at: 5278 5465 6553 2122 Size: 187 1088 5146 Fragments arranged by size: 5146 1088 688 674 519 503 428 342 187 1 1 EcoRI G'AATT_C Cuts at: 6886 7264 6886 Size: 378 9199 EcoRV GAT'ATC Cuts at: 5479 5479 Size: 9577 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2830 3721 6781 2830 Size: 891 3060 5626 Fragments arranged by size: 5626 3060 891 FseI GG_CCGG'CC Cuts at: 2013 2013 Size: 9577 FspI TGC'GCA Cuts at: 2714 6594 8083 2714 Size: 3880 1489 4208 Fragments arranged by size: 4208 3880 1489 FspAI rTGC'GCAy Cuts at: 2714 6594 2714 Size: 3880 5697 HaeII r_GCGC'y Cuts at: 1678 2392 2439 2931 3246 4477 5801 6654 8724 Size: 714 47 492 315 1231 1324 853 2070 Cuts at: 8724 1678 Size: 2531 Fragments arranged by size: 2531 2070 1324 1231 853 714 492 315 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3821 3853 3854 3886 4387 Size: 32 135 32 2249 32 1 32 501 Cuts at: 4387 4419 4568 4600 5564 5596 5970 5977 6002 Size: 32 149 32 964 32 374 7 25 Cuts at: 6002 6009 8218 8250 8292 8324 1373 Size: 7 2209 32 42 32 2626 Fragments arranged by size: 2626 2249 2209 964 501 374 149 135 42 32 32 32 32 32 32 32 32 32 25 7 7 1 HincII GTy'rAC Cuts at: 669 1657 1938 4807 6921 7167 669 Size: 988 281 2869 2114 246 3079 Fragments arranged by size: 3079 2869 2114 988 281 246 HindIII A'AGCT_T Cuts at: 748 3476 748 Size: 2728 6849 HpaI GTT'AAC Cuts at: 7167 7167 Size: 9577 KasI G'GCGC_C Cuts at: 4473 4473 Size: 9577 KpnI G_GTAC'C Cuts at: 6902 6902 Size: 9577 MfeI C'AATT_G Cuts at: 7176 7176 Size: 9577 MluI A'CGCG_T Cuts at: 9453 9453 Size: 9577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 2544 3751 5157 5806 8691 8875 1305 Size: 996 243 1207 1406 649 2885 184 2007 Fragments arranged by size: 2885 2007 1406 1207 996 649 243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4481 6318 6848 10 Size: 54 1425 343 2649 1837 530 2739 Fragments arranged by size: 2739 2649 1837 1425 530 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2654 4002 4784 5012 5060 5702 Size: 648 1038 450 1348 782 228 48 642 Cuts at: 5702 5860 6080 6477 7535 7894 8053 518 Size: 158 220 397 1058 359 159 2042 Fragments arranged by size: 2042 1348 1058 1038 782 648 642 450 397 359 228 220 159 158 48 MspA1I CmG'CkG Cuts at: 1606 2059 3679 3976 4051 4318 4378 5020 5653 Size: 453 1620 297 75 267 60 642 633 Cuts at: 5653 6993 7670 8818 9063 1606 Size: 1340 677 1148 245 2120 Fragments arranged by size: 2120 1620 1340 1148 677 642 633 453 297 267 245 75 60 NaeI GCC'GGC Cuts at: 2011 2830 3406 2011 Size: 819 576 8182 Fragments arranged by size: 8182 819 576 NarI GG'CG_CC Cuts at: 4474 4474 Size: 9577 NcoI C'CATG_G Cuts at: 513 1065 2004 2292 3603 4086 5871 513 Size: 552 939 288 1311 483 1785 4219 Fragments arranged by size: 4219 1785 1311 939 552 483 288 NdeI CA'TA_TG Cuts at: 387 387 Size: 9577 NgoMIV G'CCGG_C Cuts at: 2009 2828 3404 2009 Size: 819 576 8182 Fragments arranged by size: 8182 819 576 NheI G'CTAG_C Cuts at: 1052 3394 1052 Size: 2342 7235 NotI GC'GGCC_GC Cuts at: 7024 7024 Size: 9577 NspI r_CATG'y Cuts at: 1132 1229 2719 6935 8480 1132 Size: 97 1490 4216 1545 2229 Fragments arranged by size: 4216 2229 1545 1490 97 PasI CC'CwG_GG Cuts at: 1348 3553 1348 Size: 2205 7372 PciI A'CATG_T Cuts at: 8476 8476 Size: 9577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 4645 1108 Size: 1338 2199 6040 Fragments arranged by size: 6040 2199 1338 PfoI T'CCnGG_A Cuts at: 2997 3450 5232 6093 2997 Size: 453 1782 861 6481 Fragments arranged by size: 6481 1782 861 453 PmeI GTTT'AAAC Cuts at: 6881 6881 Size: 9577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4838 4870 7646 7678 9159 9191 4838 Size: 32 2776 32 1481 32 5224 Fragments arranged by size: 5224 2776 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 3643 4162 6553 2122 Size: 1521 519 2391 5146 Fragments arranged by size: 5146 2391 1521 519 PshAI GACnn'nnGTC Cuts at: 1613 4802 1613 Size: 3189 6388 PsiI TTA'TAA Cuts at: 7147 7147 Size: 9577 PspOMI G'GGCC_C Cuts at: 2625 3102 3300 4590 5464 2625 Size: 477 198 1290 874 6738 Fragments arranged by size: 6738 1290 874 477 198 PspXI vC'TCGA_Gb Cuts at: 2779 2779 Size: 9577 PstI C_TGCA'G Cuts at: 830 3192 4029 4074 4170 4179 4866 4899 4932 Size: 2362 837 45 96 9 687 33 33 Cuts at: 4932 5277 5862 6038 6929 830 Size: 345 585 176 891 3478 Fragments arranged by size: 3478 2362 891 837 687 585 345 176 96 45 33 33 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9577 PvuII CAG'CTG Cuts at: 2059 4051 5020 2059 Size: 1992 969 6616 Fragments arranged by size: 6616 1992 969 SacI G_AGCT'C Cuts at: 2454 2672 4405 6573 2454 Size: 218 1733 2168 5458 Fragments arranged by size: 5458 2168 1733 218 SalI G'TCGA_C Cuts at: 1936 6919 1936 Size: 4983 4594 SapI GCTCTTCn'nnn_ Cuts at: 3680 3774 3680 Size: 94 9483 SbfI CC_TGCA'GG Cuts at: 4866 6929 4866 Size: 2063 7514 ScaI AGT'ACT Cuts at: 6032 7825 9537 6032 Size: 1793 1712 6072 Fragments arranged by size: 6072 1793 1712 SexAI A'CCwGG_T Cuts at: 2733 2733 Size: 9577 SfcI C'TryA_G Cuts at: 826 1045 2968 3188 3253 3284 4025 4070 4166 Size: 219 1923 220 65 31 741 45 96 Cuts at: 4166 4175 4247 4862 4895 4928 5273 5858 6034 Size: 9 72 615 33 33 345 585 176 Cuts at: 6034 6925 8060 8741 8932 826 Size: 891 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 891 741 681 615 585 345 220 219 191 176 96 72 65 45 33 33 31 9 SfiI GGCCn_nnn'nGGCC Cuts at: 4283 6324 4283 Size: 2041 7536 SfoI GGC'GCC Cuts at: 4475 4475 Size: 9577 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9577 SmaI CCC'GGG Cuts at: 1872 2109 5606 6630 6904 1872 Size: 237 3497 1024 274 4545 Fragments arranged by size: 4545 3497 1024 274 237 SmlI C'TyrA_G Cuts at: 820 1017 1943 2779 3992 4208 4364 4745 5117 Size: 197 926 836 1213 216 156 381 372 Cuts at: 5117 5631 7681 8582 8844 820 Size: 514 2050 901 262 1553 Fragments arranged by size: 2050 1553 1213 926 901 836 514 381 372 262 216 197 156 SnaBI TAC'GTA Cuts at: 493 7368 493 Size: 6875 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9577 SphI G_CATG'C Cuts at: 1132 6935 1132 Size: 5803 3774 SspI AAT'ATT Cuts at: 5 52 7501 5 Size: 47 7449 2081 Fragments arranged by size: 7449 2081 47 StuI AGG'CCT Cuts at: 1517 2144 1517 Size: 627 8950 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 2874 2910 3399 3603 4086 Size: 552 939 288 582 36 489 204 483 Cuts at: 4086 4278 5031 5621 5835 5871 6390 7016 513 Size: 192 753 590 214 36 519 626 3074 Fragments arranged by size: 3074 939 753 626 590 582 552 519 489 483 288 214 204 192 36 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 2738 3072 3377 3545 4543 5691 Size: 1255 1086 72 334 305 168 998 1148 Cuts at: 5691 5961 6365 6595 6715 7604 7621 7774 7959 Size: 270 404 230 120 889 17 153 185 Cuts at: 7959 8404 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1148 1086 998 889 445 404 334 305 270 230 185 168 153 120 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6030 7823 9535 Size: 274 80 33 51 1443 4053 1793 1712 Cuts at: 9535 96 Size: 138 Fragments arranged by size: 4053 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2501 2533 6593 6625 9537 9569 2501 Size: 32 4060 32 2912 32 2509 Fragments arranged by size: 4060 2912 2509 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2159 4781 5576 1316 Size: 843 2622 795 5317 Fragments arranged by size: 5317 2622 843 795 XbaI T'CTAG_A Cuts at: 3481 6913 3481 Size: 3432 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3643 4807 5017 5038 5902 3643 Size: 1164 210 21 864 7318 Fragments arranged by size: 7318 1164 864 210 21 XhoI C'TCGA_G Cuts at: 1943 2779 5631 1943 Size: 836 2852 5889 Fragments arranged by size: 5889 2852 836 XmaI C'CCGG_G Cuts at: 1870 2107 5604 6628 6902 1870 Size: 237 3497 1024 274 4545 Fragments arranged by size: 4545 3497 1024 274 237 XmnI GAAnn'nnTTC Cuts at: 7706 7706 Size: 9577 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5646 7385 276 Size: 53 83 186 936 4112 1739 2468 Fragments arranged by size: 4112 2468 1739 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BfrBI BsiWI BstZ17I NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SrfI SwaI