################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FauI FseI FspI HpaI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 491 GTCGAC GT^[AC][GT]AC AciI 13 GCGG CCGC(-3/-1) AflIII 603 ACATGT A^C[GA][CT]GT AlwI 1455 GATCC GGATC(4/5) AseI 1686 ATTAAT AT^TAAT AsnI 1686 ATTAAT AT^TAAT Asp700I 1639 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 83 CCCGGG C^[CT]CG[GA]G BanI 611 GGCGCC G^G[CT][GA]CC BanII 542 GAGCCC G[GA]GC[CT]^C BbeI 611 GGCGCC GGCGC^C BbvCI 811 GCTGAGG CCTCAGC(-5/-2) BclI 1844 TGATCA T^GATCA BglII 1093 AGATCT A^GATCT BsaBI 468 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 83 CCCGGG C^[CT]CG[GA]G BspLU11I 603 ACATGT A^CATGT BsrDI 1812 CATTGC GCAATG(2/0) BstAPI 1318 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1056 GGTGACC G^GT[ACGT]ACC BstPI 1056 GGTGACC G^GT[ACGT]ACC BstXI 530 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1093 AGATCT [GA]^GATC[CT] CfoI 612 GCGC GCG^C DrdI 353 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1650 CCATGG C^C[GA][CT]GG EcoO65I 1056 GGTGACC G^GT[ACGT]ACC EcoRV 1201 GATATC GAT^ATC FbaI 1844 TGATCA T^GATCA HaeII 611 GGCGCC [GA]GCGC^[CT] HgaI 1242 GCGTC GACGC(5/10) HhaI 612 GCGC GCG^C HinP1I 612 GCGC G^CGC HindIII 406 AAGCTT A^AGCTT KasI 611 GGCGCC G^GCGCC MamI 468 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1093 AGATCT [GA]^GATC[CT] MspA1I 1528 CAGCTG C[AC]G^C[GT]G NarI 611 GGCGCC GG^CGCC NcoI 1650 CCATGG C^CATGG PflMI 1650 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1872 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1686 ATTAAT AT^TAAT PvuII 1528 CAGCTG CAG^CTG SalI 491 GTCGAC G^TCGAC SfoI 611 GGCGCC GGC^GCC SmaI 83 CCCGGG CCC^GGG SmlI 1976 CTCAAG C^T[CT][GA]AG SphI 1553 GCATGC GCATG^C Van91I 1650 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 854 CCACTGGGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1093 AGATCT [GA]^GATC[CT] XmaCI 83 CCCGGG C^CCGGG XmaI 83 CCCGGG C^CCGGG XmnI 1639 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 611 GGCGCC G[GA]^CG[CT]C 1241 GGCGTC BmyI 369 GTGCCC G[AGT]GC[ACT]^C 542 GAGCCC BsaHI 611 GGCGCC G[GA]^CG[CT]C 1241 GGCGTC BsmI 1628 GAATGC GAATGC(1/-1) 1153 GCATTC Bsp1286I 369 GTGCCC G[AGT]GC[ACT]^C 542 GAGCCC Bsp1407I 705 TGTACA T^GTACA 793 TGTACA BsrGI 705 TGTACA T^GTACA 793 TGTACA EarI 1643 CTCTTC CTCTTC(1/4) 1474 GAAGAG EcoNI 33 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 174 CCTGGCCCAGG EcoT22I 1084 ATGCAT ATGCA^T 1733 ATGCAT Hin1I 611 GGCGCC G[GA]^CG[CT]C 1241 GGCGTC HincII 491 GTCGAC GT[CT]^[GA]AC 1747 GTCAAC HindII 491 GTCGAC GT[CT]^[GA]AC 1747 GTCAAC Ksp632I 1643 CTCTTC CTCTTC(1/4) 1474 GAAGAG NsiI 1084 ATGCAT ATGCA^T 1733 ATGCAT ScaI 1353 AGTACT AGT^ACT 1882 AGTACT SspBI 705 TGTACA T^GTACA 793 TGTACA TaqI 492 TCGA T^CGA 1989 TCGA TthHB8I 492 TCGA T^CGA 1989 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AcsI AfaI AluI AlwNI AocI ApoI AvaII BbvI BcnI BfaI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI