(Circular) (Six-base) MAPSORT of: pFN21AE3286.seq Check: 9685 from: 1 to: 6904 pFN21AE3286 6904 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4714 278 Size: 53 83 186 936 3178 2468 Fragments arranged by size: 3178 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2497 4247 1937 Size: 560 1750 4594 Fragments arranged by size: 4594 1750 560 Acc65I G'GTAC_C Cuts at: 4225 4225 Size: 6904 AclI AA'CG_TT Cuts at: 1563 5031 5404 1563 Size: 3468 373 3063 Fragments arranged by size: 3468 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3408 4152 4967 6351 6619 1632 Size: 1776 744 815 1384 268 1917 Fragments arranged by size: 1917 1776 1384 815 744 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6707 AflIII A'CryG_T Cuts at: 1203 2608 5803 6780 1203 Size: 1405 3195 977 1327 Fragments arranged by size: 3195 1405 1327 977 AgeI A'CCGG_T Cuts at: 5735 5735 Size: 6904 AhdI GACnn_n'nnGTC Cuts at: 5633 5633 Size: 6904 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3355 4207 1184 Size: 2171 852 3881 Fragments arranged by size: 3881 2171 852 AlwNI CAG_nnn'CTG Cuts at: 2174 4130 4145 4647 6219 2174 Size: 1956 15 502 1572 2859 Fragments arranged by size: 2859 1956 1572 502 15 ApaLI G'TGCA_C Cuts at: 4961 6117 4961 Size: 1156 5748 ApoI r'AATT_y Cuts at: 1493 2281 2305 2387 2431 2456 2685 2935 3486 Size: 788 24 82 44 25 229 250 551 Cuts at: 3486 3856 4213 4439 4591 6700 1493 Size: 370 357 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 551 370 357 250 229 226 152 82 44 25 24 AseI AT'TA_AT Cuts at: 160 1032 3692 5458 160 Size: 872 2660 1766 1606 Fragments arranged by size: 2660 1766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6904 AvaI C'yCGr_G Cuts at: 1870 1943 2088 4229 1870 Size: 73 145 2141 4545 Fragments arranged by size: 4545 2141 145 73 BamHI G'GATC_C Cuts at: 4234 4601 4234 Size: 367 6537 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2616 4225 5680 618 Size: 325 217 627 829 1609 1455 1842 Fragments arranged by size: 1842 1609 1455 829 627 325 217 BanII G_rGCy'C Cuts at: 1391 2551 4223 1391 Size: 1160 1672 4072 Fragments arranged by size: 4072 1672 1160 BbeI G_GCGC'C Cuts at: 2620 2620 Size: 6904 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5933 BbvCI CC'TCA_GC Cuts at: 2817 2817 Size: 6904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5095 5129 1903 Size: 34 3158 34 3678 Fragments arranged by size: 3678 3158 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4796 6006 4796 Size: 1210 5694 BclI T'GATC_A Cuts at: 1548 3849 1548 Size: 2301 4603 BfrBI ATG'CAT Cuts at: 3091 3740 3091 Size: 649 6255 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5515 136 Size: 107 122 71 631 605 3843 1525 Fragments arranged by size: 3843 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3098 6899 3098 Size: 3801 3103 BlpI GC'TnA_GC Cuts at: 4321 4321 Size: 6904 Bme1580I G_kGCm'C Cuts at: 1790 2378 4965 6121 1790 Size: 588 2587 1156 2573 Fragments arranged by size: 2587 2573 1156 588 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6904 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 4635 5593 Size: 416 53 83 399 24 1454 1764 958 Cuts at: 5593 442 Size: 1753 Fragments arranged by size: 1764 1753 1454 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6904 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2145 2411 4116 5564 1239 Size: 821 85 266 1705 1448 2579 Fragments arranged by size: 2579 1705 1448 821 266 85 Bpu10I CC'TnA_GC Cuts at: 2817 2817 Size: 6904 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3966 5029 5894 6192 3966 Size: 1063 865 298 4678 Fragments arranged by size: 4678 1063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5567 882 Size: 617 4068 2219 Fragments arranged by size: 4068 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4695 493 Size: 711 3491 2702 Fragments arranged by size: 3491 2702 711 BsaBI GATnn'nnATC Cuts at: 2477 6898 2477 Size: 4421 2483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2617 3247 4711 5093 Size: 53 83 186 936 1084 630 1464 382 Cuts at: 5093 5728 275 Size: 635 1451 Fragments arranged by size: 1464 1451 1084 936 635 630 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5337 5735 6009 6156 6567 5337 Size: 398 274 147 411 5674 Fragments arranged by size: 5674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6679 Fragments arranged by size: 6679 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2039 2063 2240 1186 Size: 850 3 24 177 5850 Fragments arranged by size: 5850 850 177 24 3 BseYI C'CCAG_C Cuts at: 2099 2107 2167 6107 6603 2099 Size: 8 60 3940 496 2400 Fragments arranged by size: 3940 2400 496 60 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6331 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4354 5115 6143 1540 Size: 275 186 2353 761 1028 2301 Fragments arranged by size: 2353 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4965 5050 6121 4965 Size: 85 1071 5748 Fragments arranged by size: 5748 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3639 4415 4508 3158 Size: 481 776 93 5554 Fragments arranged by size: 5554 776 481 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2378 2551 4223 4965 5050 6121 1391 Size: 399 588 173 1672 742 85 1071 2174 Fragments arranged by size: 2174 1672 1071 742 588 399 173 85 BspEI T'CCGG_A Cuts at: 6567 6567 Size: 6904 BspHI T'CATG_A Cuts at: 4793 6523 4793 Size: 1730 5174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4259 844 Size: 3415 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4791 1394 Size: 429 2968 3507 Fragments arranged by size: 3507 2968 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3816 5399 5573 65 Size: 1183 2568 1583 174 1396 Fragments arranged by size: 2568 1583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5548 5735 1700 Size: 110 3738 187 2869 Fragments arranged by size: 3738 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2710 2798 96 Size: 2614 88 4202 Fragments arranged by size: 4202 2614 88 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6904 BssSI C'ACGA_G Cuts at: 4964 5976 4964 Size: 1012 5892 BstAPI GCAn_nnn'nTGC Cuts at: 794 3329 794 Size: 2535 4369 BstBI TT'CG_AA Cuts at: 5708 5708 Size: 6904 BstEII G'GTnAC_C Cuts at: 1184 3061 1184 Size: 1877 5027 BstXI CCAn_nnnn'nTGG Cuts at: 2542 2542 Size: 6904 BstYI r'GATC_y Cuts at: 1972 3098 4234 4601 4986 5003 6455 6541 6553 Size: 1126 1136 367 385 17 1452 86 12 Cuts at: 6553 6899 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1136 1126 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2223 2379 2679 2223 Size: 156 300 6448 Fragments arranged by size: 6448 300 156 BtgI C'CryG_G Cuts at: 513 1065 2004 3655 513 Size: 552 939 1651 3762 Fragments arranged by size: 3762 1651 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6165 Fragments arranged by size: 6165 683 56 BtsI GCAGTG_nn' Cuts at: 2664 3324 3375 4420 5214 5234 2664 Size: 660 51 1045 794 20 4334 Fragments arranged by size: 4334 1045 794 660 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6904 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6869 DraI TTT'AAA Cuts at: 2970 4057 4071 4208 2970 Size: 1087 14 137 5666 Fragments arranged by size: 5666 1087 137 14 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2364 5911 809 Size: 507 1048 3547 1802 Fragments arranged by size: 3547 1802 1048 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4351 5240 6869 Size: 54 1425 50 275 18 2521 889 1629 Cuts at: 6869 8 Size: 43 Fragments arranged by size: 2521 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4351 1537 Size: 275 2539 4090 Fragments arranged by size: 4090 2539 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3474 3654 4834 1353 Size: 2121 180 1180 3423 Fragments arranged by size: 3423 2121 1180 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 5491 5865 6011 387 Size: 1064 138 444 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 444 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2145 2411 3408 4116 4152 4967 Size: 393 428 85 266 997 708 36 815 Cuts at: 4967 5564 6351 6619 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 997 815 787 708 597 428 393 268 266 85 36 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2183 2042 Size: 141 6763 EcoO109I rG'GnC_Cy Cuts at: 2074 2104 3416 2074 Size: 30 1312 5562 Fragments arranged by size: 5562 1312 30 EcoRI G'AATT_C Cuts at: 4213 4591 4213 Size: 378 6526 EcoRV GAT'ATC Cuts at: 3208 3208 Size: 6904 FspI TGC'GCA Cuts at: 5410 5410 Size: 6904 HaeII r_GCGC'y Cuts at: 1678 2620 6051 1678 Size: 942 3431 2531 Fragments arranged by size: 3431 2531 942 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 3451 3483 5545 Size: 32 135 32 1055 32 792 32 2062 Cuts at: 5545 5577 5619 5651 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2062 1055 792 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2498 3754 4248 4494 669 Size: 988 281 560 1256 494 246 3079 Fragments arranged by size: 3079 1256 988 560 494 281 246 HindIII A'AGCT_T Cuts at: 748 2411 748 Size: 1663 5241 HpaI GTT'AAC Cuts at: 4494 4494 Size: 6904 KasI G'GCGC_C Cuts at: 2616 2616 Size: 6904 KpnI G_GTAC'C Cuts at: 4229 4229 Size: 6904 MfeI C'AATT_G Cuts at: 4503 4503 Size: 6904 MluI A'CGCG_T Cuts at: 6780 6780 Size: 6904 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2168 6018 6202 1305 Size: 863 3850 184 2007 Fragments arranged by size: 3850 2007 863 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5082 Fragments arranged by size: 5082 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4862 5221 5380 518 Size: 648 3696 359 159 2042 Fragments arranged by size: 3696 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3535 4320 4997 6145 6390 1606 Size: 1929 785 677 1148 245 2120 Fragments arranged by size: 2120 1929 1148 785 677 245 NarI GG'CG_CC Cuts at: 2617 2617 Size: 6904 NcoI C'CATG_G Cuts at: 513 1065 2004 3655 513 Size: 552 939 1651 3762 Fragments arranged by size: 3762 1651 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6904 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6904 NotI GC'GGCC_GC Cuts at: 4351 4351 Size: 6904 NsiI A_TGCA'T Cuts at: 3093 3742 3093 Size: 649 6255 NspI r_CATG'y Cuts at: 1132 1229 2612 3239 3562 4262 5807 1132 Size: 97 1383 627 323 700 1545 2229 Fragments arranged by size: 2229 1545 1383 700 627 323 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6904 PciI A'CATG_T Cuts at: 2608 5803 2608 Size: 3195 3709 PflMI CCAn_nnn'nTGG Cuts at: 1108 3661 1108 Size: 2553 4351 PmeI GTTT'AAAC Cuts at: 4208 4208 Size: 6904 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4973 5005 6486 6518 4973 Size: 32 1481 32 5359 Fragments arranged by size: 5359 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2074 2104 3416 2074 Size: 30 1312 5562 Fragments arranged by size: 5562 1312 30 PshAI GACnn'nnGTC Cuts at: 1613 3881 1613 Size: 2268 4636 PsiI TTA'TAA Cuts at: 4474 4474 Size: 6904 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2554 2586 2554 Size: 32 6872 PstI C_TGCA'G Cuts at: 830 3319 3334 4196 4256 830 Size: 2489 15 862 60 3478 Fragments arranged by size: 3478 2489 862 60 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6904 PvuII CAG'CTG Cuts at: 3535 3535 Size: 6904 SalI G'TCGA_C Cuts at: 1936 2496 4246 1936 Size: 560 1750 4594 Fragments arranged by size: 4594 1750 560 SanDI GG'GwC_CC Cuts at: 2104 2104 Size: 6904 SbfI CC_TGCA'GG Cuts at: 4256 4256 Size: 6904 ScaI AGT'ACT Cuts at: 3360 3889 5152 6864 3360 Size: 529 1263 1712 3400 Fragments arranged by size: 3400 1712 1263 529 SfcI C'TryA_G Cuts at: 826 1045 3201 3315 3330 3789 4192 4252 5387 Size: 219 2156 114 15 459 403 60 1135 Cuts at: 5387 6068 6259 826 Size: 681 191 1471 Fragments arranged by size: 2156 1471 1135 681 459 403 219 191 114 60 15 SfoI GGC'GCC Cuts at: 2618 2618 Size: 6904 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6904 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6904 SmaI CCC'GGG Cuts at: 1872 2090 4231 1872 Size: 218 2141 4545 Fragments arranged by size: 4545 2141 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 3981 5008 5909 6171 820 Size: 197 926 2038 1027 901 262 1553 Fragments arranged by size: 2038 1553 1027 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4695 493 Size: 4202 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6904 SphI G_CATG'C Cuts at: 1132 3562 4262 1132 Size: 2430 700 3774 Fragments arranged by size: 3774 2430 700 SspI AAT'ATT Cuts at: 5 52 2793 3055 3281 4828 5 Size: 47 2741 262 226 1547 2081 Fragments arranged by size: 2741 2081 1547 262 226 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6904 StyI C'CwwG_G Cuts at: 513 1065 2004 3178 3225 3655 4343 513 Size: 552 939 1174 47 430 688 3074 Fragments arranged by size: 3074 1174 939 688 552 430 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4013 4931 4948 5101 5286 5731 325 Size: 1255 2433 918 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2710 2798 3082 Size: 274 80 33 51 1443 733 88 284 Cuts at: 3082 3358 3887 4074 5150 6862 96 Size: 276 529 187 1076 1712 138 Fragments arranged by size: 1712 1443 1076 733 529 284 276 274 187 138 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 6864 6896 3123 Size: 32 3709 32 3131 Fragments arranged by size: 3709 3131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6904 XbaI T'CTAG_A Cuts at: 2275 2842 3118 3148 4240 2275 Size: 567 276 30 1092 4939 Fragments arranged by size: 4939 1092 567 276 30 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2866 2011 Size: 855 6049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6904 XmaI C'CCGG_G Cuts at: 1870 2088 4229 1870 Size: 218 2141 4545 Fragments arranged by size: 4545 2141 218 XmnI GAAnn'nnTTC Cuts at: 3648 5033 3648 Size: 1385 5519 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4712 276 Size: 53 83 186 936 3178 2468 Fragments arranged by size: 3178 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI