################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BsgI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI HindIII HinfI HpaI KpnI KspI MamI MfeI MluI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 306 CGCG CG^CG AccIII 968 TCCGGA T^CCGGA Alw44I 675 GTGCAC G^TGCAC AlwI 161 GGATC GGATC(4/5) AocI 210 CCTAAGG CC^T[ACGT]AGG ApaLI 675 GTGCAC G^TGCAC AvaI 610 CCCGAG C^[CT]CG[GA]G AviII 707 TGCGCA TGC^GCA BbeI 216 GGCGCC GGCGC^C BbsI 228 GAAGAC GAAGAC(2/6) BbvCI 909 CCTCAGC CCTCAGC(-5/-2) BlpI 594 GCTGAGC GC^T[ACGT]AGC Bpu1102I 594 GCTGAGC GC^T[ACGT]AGC BpuAI 228 GAAGAC GAAGAC(2/6) BsaWI 968 TCCGGA [AT]^CCGG[AT] BseAI 968 TCCGGA T^CCGGA BseRI 808 CTCCTC GAGGAG(10/8) BsiEI 191 CGGCCG CG[GA][CT]^CG BsmAI 73 GTCTC GTCTC(1/5) BsmBI 72 CGTCTC CGTCTC(1/5) BsoBI 610 CCCGAG C^[CT]CG[GA]G BspEI 968 TCCGGA T^CCGGA BsrBI 732 CCGCTC CCGCTC(-3/-3) BssSI 924 CTCGTG CACGAG(-5/-1) BstAPI 312 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 306 CGCG CG^CG BstYI 161 GGATCT [GA]^GATC[CT] Bsu36I 210 CCTAAGG CC^T[ACGT]AGG CelII 594 GCTGAGC GC^T[ACGT]AGC DpnII 162 GATC ^GATC EagI 191 CGGCCG C^GGCCG EarI 277 CTCTTC CTCTTC(1/4) EclXI 191 CGGCCG C^GGCCG Eco52I 191 CGGCCG C^GGCCG Eco81I 210 CCTAAGG CC^T[ACGT]AGG EcoT14I 743 CCAAGG C^C[AT][AT]GG FspI 707 TGCGCA TGC^GCA HaeII 216 GGCGCC [GA]GCGC^[CT] KasI 216 GGCGCC G^GCGCC Ksp632I 277 CTCTTC CTCTTC(1/4) MboI 162 GATC ^GATC MflI 161 GGATCT [GA]^GATC[CT] MroI 968 TCCGGA T^CCGGA MspA1I 8 CCGCTG C[AC]G^C[GT]G MvnI 306 CGCG CG^CG NarI 216 GGCGCC GG^CGCC NdeII 162 GATC ^GATC NlaIII 462 CATG CATG^ NspI 461 ACATGC [GA]CATG^[CT] PflMI 583 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SalI 367 GTCGAC G^TCGAC SapI 276 GCTCTTC GCTCTTC(1/4) Sau3AI 162 GATC ^GATC ScaI 323 AGTACT AGT^ACT SexAI 428 ACCTGGT A^CC[AT]GGT SfaNI 392 GCATC GCATC(5/9) SfoI 216 GGCGCC GGC^GCC StyI 743 CCAAGG C^C[AT][AT]GG TaqI 368 TCGA T^CGA TthHB8I 368 TCGA T^CGA Van91I 583 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 954 TCTAGA T^CTAGA XhoII 161 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 242 ACGTGT A^C[GA][CT]GT 644 ACACGT Asp700I 933 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 972 GAAGAACTTC BalI 93 TGGCCA TGG^CCA 440 TGGCCA BcnI 158 CCGGG CC^[GC]GG 480 CCGGG BpmI 387 CTCCAG CTGGAG(16/14) 737 CTGGAG BsmFI 453 GTCCC GGGAC(10/14) 580 GTCCC BstEII 234 GGTGACC G^GT[ACGT]ACC 771 GGTGACC BstF5I 198 CATCC GGATG(2/0) 249 CATCC BstPI 234 GGTGACC G^GT[ACGT]ACC 771 GGTGACC DraII 451 AGGTCCC [GA]G^G[ACGT]CC[CT] 578 AGGTCCC DsaI 604 CCGTGG C^C[GA][CT]GG 854 CCACGG EcoO109I 451 AGGTCCC [GA]G^G[ACGT]CC[CT] 578 AGGTCCC EcoO65I 234 GGTGACC G^GT[ACGT]ACC 771 GGTGACC FokI 198 CATCC GGATG(9/13) 249 CATCC HincII 298 GTCAAC GT[CT]^[GA]AC 367 GTCGAC HindII 298 GTCAAC GT[CT]^[GA]AC 367 GTCGAC MluNI 93 TGGCCA TGG^CCA 440 TGGCCA MscI 93 TGGCCA TGG^CCA 440 TGGCCA MslI 681 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 882 CATCAGTATG NciI 158 CCGGG CC^[GC]GG 480 CCGGG PpuMI 451 AGGTCCC [GA]G^G[AT]CC[CT] 578 AGGTCCC PstI 319 CTGCAG CTGCA^G 574 CTGCAG XmnI 933 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 972 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AspHI AvaII BanI BanII BbvI BfaI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeI MaeII MaeIII MboII MnlI MspI MvaI MwoI NlaIV RsaI Sau96I ScrFI SfcI TseI Tsp45I TspRI