(Circular) (Six-base) MAPSORT of: pFN21AE9502.seq Check: 4497 from: 1 to: 5704 pFN21AE9502 5704 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2328 2328 Size: 5704 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 2565 2646 3047 1937 Size: 436 192 81 401 4594 Fragments arranged by size: 4594 436 401 192 81 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2247 2649 4603 5580 1203 Size: 1044 402 1954 977 1327 Fragments arranged by size: 1954 1327 1044 977 402 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 4433 4433 Size: 5704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2378 2594 3011 1184 Size: 1194 216 417 3877 Fragments arranged by size: 3877 1194 417 216 AlwNI CAG_nnn'CTG Cuts at: 3447 5019 3447 Size: 1572 4132 ApaLI G'TGCA_C Cuts at: 2680 3761 4917 2680 Size: 1081 1156 3467 Fragments arranged by size: 3467 1156 1081 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2615 3029 1870 Size: 73 672 414 4545 Fragments arranged by size: 4545 672 414 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2069 2221 3025 4480 Size: 325 217 627 232 50 152 804 1455 Cuts at: 4480 618 Size: 1842 Fragments arranged by size: 1842 1455 804 627 325 232 217 152 50 BanII G_rGCy'C Cuts at: 1391 2031 2048 2283 3023 1391 Size: 640 17 235 740 4072 Fragments arranged by size: 4072 740 640 235 17 BbeI G_GCGC'C Cuts at: 2225 2225 Size: 5704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2240 928 Size: 971 341 4392 Fragments arranged by size: 4392 971 341 BbvCI CC'TCA_GC Cuts at: 2915 2915 Size: 5704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3895 3929 1903 Size: 34 50 34 1874 34 3678 Fragments arranged by size: 3678 1874 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4315 136 Size: 107 122 71 631 605 337 2306 1525 Fragments arranged by size: 2306 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5699 5699 Size: 5704 BlpI GC'TnA_GC Cuts at: 2600 3121 2600 Size: 521 5183 Bme1580I G_kGCm'C Cuts at: 1790 2022 2684 3765 4921 1790 Size: 232 662 1081 1156 2573 Fragments arranged by size: 2573 1156 1081 662 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3435 4393 442 Size: 416 53 83 399 24 2018 958 1753 Fragments arranged by size: 2018 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2377 2763 4364 1239 Size: 1138 386 1601 2579 Fragments arranged by size: 2579 1601 1138 386 Bpu10I CC'TnA_GC Cuts at: 2915 2915 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2222 2523 2562 2825 Size: 53 83 186 936 689 301 39 263 Cuts at: 2825 3511 3893 4528 275 Size: 686 382 635 1451 Fragments arranged by size: 1451 936 689 686 635 382 301 263 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2973 4137 4535 4809 4956 5367 2973 Size: 1164 398 274 147 411 3310 Fragments arranged by size: 3310 1164 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 1178 Size: 30 165 30 972 30 4477 Fragments arranged by size: 4477 972 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 1186 Size: 1618 4086 BseYI C'CCAG_C Cuts at: 2015 2518 4907 5403 2015 Size: 503 2389 496 2316 Fragments arranged by size: 2389 2316 503 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5131 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 3154 3915 4943 1540 Size: 275 186 198 955 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 275 198 186 BsiHKAI G_wGCw'C Cuts at: 2145 2319 2684 2697 3765 3850 4921 2145 Size: 174 365 13 1068 85 1071 2928 Fragments arranged by size: 2928 1071 1068 365 174 85 13 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 BsmBI CGTCTCn'nnnn_ Cuts at: 2083 2083 Size: 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2031 2048 2145 2283 2319 2684 Size: 399 232 9 17 97 138 36 365 Cuts at: 2684 2697 3023 3765 3850 4921 1391 Size: 13 326 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 365 326 232 138 97 85 36 17 13 9 BspEI T'CCGG_A Cuts at: 2973 5367 2973 Size: 2394 3310 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2572 2611 2874 3059 844 Size: 1484 244 39 263 185 3489 Fragments arranged by size: 3489 1484 263 244 185 39 BsrBI CCG'CTC Cuts at: 1394 1823 2739 3591 1394 Size: 429 916 852 3507 Fragments arranged by size: 3507 916 852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4348 4535 1700 Size: 110 2538 187 2869 Fragments arranged by size: 2869 2538 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 2929 3764 4776 2929 Size: 835 1012 3857 Fragments arranged by size: 3857 1012 835 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 794 Size: 1529 4175 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 2239 2776 1184 Size: 1055 537 4112 Fragments arranged by size: 4112 1055 537 BstYI r'GATC_y Cuts at: 1972 2166 3034 3401 3786 3803 5255 5341 5353 Size: 194 868 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 868 385 367 346 194 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2216 2216 Size: 5704 BtgI C'CryG_G Cuts at: 513 1065 2609 2859 513 Size: 552 1544 250 3358 Fragments arranged by size: 3358 1544 552 250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2293 487 Size: 683 56 1067 3898 Fragments arranged by size: 3898 1067 683 56 BtsI GCAGTG_nn' Cuts at: 2123 3220 4014 4034 2123 Size: 1097 794 20 3793 Fragments arranged by size: 3793 1097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2196 2265 Size: 54 1425 50 275 18 268 98 69 Cuts at: 2265 2367 2445 2802 3151 4040 5669 8 Size: 102 78 357 349 889 1629 43 Fragments arranged by size: 1629 1425 889 357 349 275 268 102 98 78 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2196 3151 1537 Size: 275 384 955 4090 Fragments arranged by size: 4090 955 384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2288 3634 1353 Size: 935 1346 3423 Fragments arranged by size: 3423 1346 935 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2785 4291 4665 4811 387 Size: 1064 138 1196 1506 374 146 1280 Fragments arranged by size: 1506 1280 1196 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2377 2763 3767 4364 5151 5419 1239 Size: 393 745 386 1004 597 787 268 1524 Fragments arranged by size: 1524 1004 787 745 597 393 386 268 EcoO109I rG'GnC_Cy Cuts at: 2457 2584 2457 Size: 127 5577 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FspI TGC'GCA Cuts at: 2714 4210 2714 Size: 1496 4208 HaeII r_GCGC'y Cuts at: 1678 2225 4851 1678 Size: 547 2626 2531 Fragments arranged by size: 2626 2531 547 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373 Size: 32 135 32 2773 32 42 32 2626 Fragments arranged by size: 2773 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2305 2374 3048 3294 669 Size: 988 281 367 69 674 246 3079 Fragments arranged by size: 3079 988 674 367 281 246 69 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2221 2221 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 2447 10 Size: 54 1425 343 268 347 3267 Fragments arranged by size: 3267 1425 347 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 2891 3662 4021 4180 518 Size: 648 1524 201 771 359 159 2042 Fragments arranged by size: 2042 1524 771 648 359 201 159 MspA1I CmG'CkG Cuts at: 1606 2015 3120 3797 4945 5190 1606 Size: 409 1105 677 1148 245 2120 Fragments arranged by size: 2120 1148 1105 677 409 245 NarI GG'CG_CC Cuts at: 2222 2222 Size: 5704 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5152 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 2470 3062 4607 1132 Size: 97 1241 592 1545 2229 Fragments arranged by size: 2229 1545 1241 592 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2594 1108 Size: 1486 4218 PfoI T'CCnGG_A Cuts at: 2162 2162 Size: 5704 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2457 2584 2457 Size: 127 5577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PstI C_TGCA'G Cuts at: 830 2328 2583 3056 830 Size: 1498 255 473 3478 Fragments arranged by size: 3478 1498 473 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SalI G'TCGA_C Cuts at: 1936 2372 3046 1936 Size: 436 674 4594 Fragments arranged by size: 4594 674 436 SapI GCTCTTCn'nnn_ Cuts at: 2288 2288 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 2330 3952 5664 2330 Size: 1622 1712 2370 Fragments arranged by size: 2370 1712 1622 SexAI A'CCwGG_T Cuts at: 2433 2433 Size: 5704 SfcI C'TryA_G Cuts at: 826 1045 2324 2566 2579 3052 4187 4868 5059 Size: 219 1279 242 13 473 1135 681 191 Cuts at: 5059 826 Size: 1471 Fragments arranged by size: 1471 1279 1135 681 473 242 219 191 13 SfoI GGC'GCC Cuts at: 2223 2223 Size: 5704 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5704 StyI C'CwwG_G Cuts at: 513 1065 2748 3143 513 Size: 552 1683 395 3074 Fragments arranged by size: 3074 1683 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2037 2057 2442 3731 3748 3901 4086 Size: 1255 457 20 385 1289 17 153 185 Cuts at: 4086 4531 325 Size: 445 1498 Fragments arranged by size: 1498 1289 1255 457 445 385 185 153 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 2328 3950 Size: 274 80 33 51 1443 180 171 1622 Cuts at: 3950 5662 96 Size: 1712 138 Fragments arranged by size: 1712 1622 1443 274 180 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2115 2147 5664 5696 2115 Size: 32 3517 32 2123 Fragments arranged by size: 3517 2123 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 2959 3040 2959 Size: 81 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 2942 2981 3833 2942 Size: 39 852 4813 Fragments arranged by size: 4813 852 39 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI