(Circular) (Six-base) MAPSORT of: pFN21AE9462.seq Check: 9914 from: 1 to: 6163 pFN21AE9462 6163 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 3506 1937 Size: 990 579 4594 Fragments arranged by size: 4594 990 579 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3390 4226 5610 5878 1632 Size: 1758 836 1384 268 1917 Fragments arranged by size: 1917 1758 1384 836 268 AfeI AGC'GCT Cuts at: 2849 2849 Size: 6163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 3084 3375 3453 5062 6039 1203 Size: 1881 291 78 1609 977 1327 Fragments arranged by size: 1881 1609 1327 977 291 78 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 2333 1166 Size: 1167 4996 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2123 3235 3906 5478 2123 Size: 1112 671 1572 2808 Fragments arranged by size: 2808 1572 1112 671 ApaI G_GGCC'C Cuts at: 2209 2592 2209 Size: 383 5780 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2592 3032 3096 3488 1870 Size: 73 649 440 64 392 4545 Fragments arranged by size: 4545 649 440 392 73 64 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2804 3484 4939 618 Size: 325 217 627 1017 680 1455 1842 Fragments arranged by size: 1842 1455 1017 680 627 325 217 BanII G_rGCy'C Cuts at: 1391 2209 2592 3482 1391 Size: 818 383 890 4072 Fragments arranged by size: 4072 890 818 383 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3378 928 Size: 971 1479 3713 Fragments arranged by size: 3713 1479 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4354 4388 1903 Size: 34 50 34 2333 34 3678 Fragments arranged by size: 3678 2333 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 2664 1548 Size: 1116 5047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2141 2706 Size: 107 122 71 631 605 377 92 565 Cuts at: 2706 4774 136 Size: 2068 1525 Fragments arranged by size: 2068 1525 631 605 565 377 122 107 92 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 2097 3580 2097 Size: 1483 4680 Bme1580I G_kGCm'C Cuts at: 1790 2209 2592 4224 5380 1790 Size: 419 383 1632 1156 2573 Fragments arranged by size: 2573 1632 1156 419 383 BmgBI CAC'GTC Cuts at: 1318 2551 1318 Size: 1233 4930 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3894 4852 442 Size: 416 53 83 399 24 2477 958 1753 Fragments arranged by size: 2477 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2318 4823 1239 Size: 885 194 2505 2579 Fragments arranged by size: 2579 2505 885 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3043 3107 4826 882 Size: 617 1544 64 1719 2219 Fragments arranged by size: 2219 1719 1544 617 64 BsaAI yAC'GTr Cuts at: 493 1204 3454 3954 493 Size: 711 2250 500 2702 Fragments arranged by size: 2702 2250 711 500 BsaBI GATnn'nnATC Cuts at: 2144 6157 2144 Size: 4013 2150 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3970 4352 4987 275 Size: 53 83 186 936 2437 382 635 1451 Fragments arranged by size: 2437 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5938 Fragments arranged by size: 5938 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2867 1186 Size: 1681 4482 BseYI C'CCAG_C Cuts at: 2309 2398 2598 2724 2800 5366 5862 2309 Size: 89 200 126 76 2566 496 2610 Fragments arranged by size: 2610 2566 496 200 126 89 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 1163 Size: 573 657 4933 Fragments arranged by size: 4933 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2877 4224 4309 5380 2340 Size: 537 1347 85 1071 3123 Fragments arranged by size: 3123 1347 1071 537 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2209 2340 2592 2877 3482 4224 4309 Size: 399 419 131 252 285 605 742 85 Cuts at: 4309 5380 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 605 419 399 285 252 131 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3518 844 Size: 2674 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2274 4050 1394 Size: 429 451 1776 3507 Fragments arranged by size: 3507 1776 451 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 3181 3199 4807 4994 1700 Size: 110 233 1138 18 1608 187 2869 Fragments arranged by size: 2869 1608 1138 233 187 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 2447 4223 5235 2447 Size: 1776 1012 3375 Fragments arranged by size: 3375 1776 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6163 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 3046 1184 Size: 1862 4301 BstXI CCAn_nnnn'nTGG Cuts at: 2202 2350 2908 3262 2202 Size: 148 558 354 5103 Fragments arranged by size: 5103 558 354 148 BstYI r'GATC_y Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158 Size: 1521 367 385 17 1452 86 12 346 Cuts at: 6158 1972 Size: 1977 Fragments arranged by size: 1977 1521 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2928 2928 Size: 6163 Bsu36I CC'TnA_GG Cuts at: 2703 2703 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2304 2584 2730 3171 3314 513 Size: 552 1239 280 146 441 143 3362 Fragments arranged by size: 3362 1239 552 441 280 146 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 2968 487 Size: 683 56 1508 234 3682 Fragments arranged by size: 3682 1508 683 234 56 BtsI GCAGTG_nn' Cuts at: 2692 3352 3679 4473 4493 2692 Size: 660 327 794 20 4362 Fragments arranged by size: 4362 794 660 327 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2878 2913 529 Size: 35 2314 35 3779 Fragments arranged by size: 3779 2314 35 35 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2355 2836 Size: 54 1425 50 275 18 312 213 481 Cuts at: 2836 2979 3402 3610 4499 6128 8 Size: 143 423 208 889 1629 43 Fragments arranged by size: 1629 1425 889 481 423 312 275 213 208 143 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2444 3023 4093 1353 Size: 836 255 579 1070 3423 Fragments arranged by size: 3423 1070 836 579 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2318 3390 4226 4823 5610 5878 Size: 393 492 194 1072 836 597 787 268 Cuts at: 5878 1239 Size: 1524 Fragments arranged by size: 1524 1072 836 787 597 492 393 268 194 EcoNI CCTnn'n_nnAGG Cuts at: 2636 2636 Size: 6163 EcoO109I rG'GnC_Cy Cuts at: 2109 2199 2403 2427 2589 2817 2109 Size: 90 204 24 162 228 5455 Fragments arranged by size: 5455 228 204 162 90 24 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 2491 Size: 6163 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2500 2851 5310 1678 Size: 822 351 2459 2531 Fragments arranged by size: 2531 2459 822 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 3365 3397 4804 Size: 32 135 32 594 32 1167 32 1407 Cuts at: 4804 4836 4878 4910 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1407 1167 594 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2024 3507 3753 669 Size: 988 281 86 1483 246 3079 Fragments arranged by size: 3079 1483 988 281 246 86 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5277 5461 1305 Size: 3972 184 2007 Fragments arranged by size: 3972 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2357 2981 10 Size: 54 1425 343 312 213 624 3192 Fragments arranged by size: 3192 1425 624 343 312 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2351 2906 3149 3260 4121 4480 Size: 648 1167 18 555 243 111 861 359 Cuts at: 4480 4639 518 Size: 159 2042 Fragments arranged by size: 2042 1167 861 648 555 359 243 159 111 18 MspA1I CmG'CkG Cuts at: 1606 3216 3232 3579 4256 5404 5649 1606 Size: 1610 16 347 677 1148 245 2120 Fragments arranged by size: 2120 1610 1148 677 347 245 16 NaeI GCC'GGC Cuts at: 2045 2045 Size: 6163 NcoI C'CATG_G Cuts at: 513 1065 3171 513 Size: 552 2106 3505 Fragments arranged by size: 3505 2106 552 NdeI CA'TA_TG Cuts at: 387 2903 387 Size: 2516 3647 NgoMIV G'CCGG_C Cuts at: 2043 2043 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3088 3148 3379 3521 5066 1132 Size: 97 1859 60 231 142 1545 2229 Fragments arranged by size: 2229 1859 1545 231 142 97 60 PasI CC'CwG_GG Cuts at: 1348 2195 2209 2431 1348 Size: 847 14 222 5080 Fragments arranged by size: 5080 847 222 14 PciI A'CATG_T Cuts at: 3084 3375 5062 3084 Size: 291 1687 4185 Fragments arranged by size: 4185 1687 291 PflMI CCAn_nnn'nTGG Cuts at: 1108 2123 3003 3340 1108 Size: 1015 880 337 3931 Fragments arranged by size: 3931 1015 880 337 PfoI T'CCnGG_A Cuts at: 2295 2295 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2109 2403 2427 2109 Size: 294 24 5845 Fragments arranged by size: 5845 294 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2205 2588 2205 Size: 383 5780 PstI C_TGCA'G Cuts at: 830 2234 3515 830 Size: 1404 1281 3478 Fragments arranged by size: 3478 1404 1281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 3232 3232 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SanDI GG'GwC_CC Cuts at: 2109 2403 2427 2109 Size: 294 24 5845 Fragments arranged by size: 5845 294 24 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2230 3511 4646 5327 5518 826 Size: 219 1185 1281 1135 681 191 1471 Fragments arranged by size: 1471 1281 1185 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 2594 3098 3490 1872 Size: 722 504 392 4545 Fragments arranged by size: 4545 722 504 392 SmlI C'TyrA_G Cuts at: 820 1017 1943 4267 5168 5430 820 Size: 197 926 2324 901 262 1553 Fragments arranged by size: 2324 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 2241 3164 1517 Size: 724 923 4516 Fragments arranged by size: 4516 923 724 StyI C'CwwG_G Cuts at: 513 1065 2157 2236 3171 3602 513 Size: 552 1092 79 935 431 3074 Fragments arranged by size: 3074 1092 935 552 431 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2590 2792 2896 4190 4207 4360 4545 Size: 1255 1010 202 104 1294 17 153 185 Cuts at: 4545 4990 325 Size: 445 1498 Fragments arranged by size: 1498 1294 1255 1010 445 202 185 153 104 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 3378 4409 Size: 274 80 33 51 1443 276 1125 1031 Cuts at: 4409 6121 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1125 1031 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6123 6155 6123 Size: 32 6131 Tth111I GACn'n_nGTC Cuts at: 1316 2765 3052 1316 Size: 1449 287 4427 Fragments arranged by size: 4427 1449 287 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2120 2350 2755 3100 2120 Size: 230 405 345 5183 Fragments arranged by size: 5183 405 345 230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 2592 3096 3488 1870 Size: 722 504 392 4545 Fragments arranged by size: 4545 722 504 392 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI