(Linear) (Six-base) MAPSORT of: KIEE9462.seq Check: 2313 from: 1 to: 1458 KIEE9462.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AccI GT'mk_AC Cuts at: 0 922 1458 Size: 922 536 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1385 1458 Size: 1385 73 AfeI AGC'GCT Cuts at: 0 844 1458 Size: 844 614 AflIII A'CryG_T Cuts at: 0 1079 1370 1448 1458 Size: 1079 291 78 10 Fragments arranged by size: 1079 291 78 10 AleI CACnn'nnGTG Cuts at: 0 328 1458 Size: 328 1130 AlwNI CAG_nnn'CTG Cuts at: 0 118 1230 1458 Size: 118 1112 228 Fragments arranged by size: 1112 228 118 ApaI G_GGCC'C Cuts at: 0 204 587 1458 Size: 204 383 871 Fragments arranged by size: 871 383 204 AvaI C'yCGr_G Cuts at: 0 587 1027 1091 1458 Size: 587 440 64 367 Fragments arranged by size: 587 440 367 64 BanI G'GyrC_C Cuts at: 0 799 1458 Size: 799 659 BanII G_rGCy'C Cuts at: 0 204 587 1458 Size: 204 383 871 Fragments arranged by size: 871 383 204 BbsI GAAGACnn'nnnn_ Cuts at: 0 1373 1458 Size: 1373 85 BclI T'GATC_A Cuts at: 0 659 1458 Size: 659 799 BglI GCCn_nnn'nGGC Cuts at: 0 44 136 701 1458 Size: 44 92 565 757 Fragments arranged by size: 757 565 92 44 BlpI GC'TnA_GC Cuts at: 0 92 1458 Size: 92 1366 Bme1580I G_kGCm'C Cuts at: 0 204 587 1458 Size: 204 383 871 Fragments arranged by size: 871 383 204 BmgBI CAC'GTC Cuts at: 0 546 1458 Size: 546 912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 313 1458 Size: 119 194 1145 Fragments arranged by size: 1145 194 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 1038 1102 1458 Size: 1038 64 356 Fragments arranged by size: 1038 356 64 BsaAI yAC'GTr Cuts at: 0 1449 1458 Size: 1449 9 BsaBI GATnn'nnATC Cuts at: 0 139 1458 Size: 139 1319 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 862 1458 Size: 862 596 BseYI C'CCAG_C Cuts at: 0 304 393 593 719 795 1458 Size: 304 89 200 126 76 663 Fragments arranged by size: 663 304 200 126 89 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1458 Size: 388 1070 BsiHKAI G_wGCw'C Cuts at: 0 335 872 1458 Size: 335 537 586 Fragments arranged by size: 586 537 335 Bsp1286I G_dGCh'C Cuts at: 0 204 335 587 872 1458 Size: 204 131 252 285 586 Fragments arranged by size: 586 285 252 204 131 BsrBI CCG'CTC Cuts at: 0 269 1458 Size: 269 1189 BsrFI r'CCGG_y Cuts at: 0 38 1176 1194 1458 Size: 38 1138 18 264 Fragments arranged by size: 1138 264 38 18 BssSI C'ACGA_G Cuts at: 0 442 1458 Size: 442 1016 BstEII G'GTnAC_C Cuts at: 0 1041 1458 Size: 1041 417 BstXI CCAn_nnnn'nTGG Cuts at: 0 197 345 903 1257 1458 Size: 197 148 558 354 201 Fragments arranged by size: 558 354 201 197 148 BstZ17I GTA'TAC Cuts at: 0 923 1458 Size: 923 535 Bsu36I CC'TnA_GG Cuts at: 0 698 1458 Size: 698 760 BtgI C'CryG_G Cuts at: 0 299 579 725 1166 1309 1458 Size: 299 280 146 441 143 149 Fragments arranged by size: 441 299 280 149 146 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 729 963 1458 Size: 729 234 495 Fragments arranged by size: 729 495 234 BtsI GCAGTG_nn' Cuts at: 0 687 1347 1458 Size: 687 660 111 Fragments arranged by size: 687 660 111 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 873 908 1458 Size: 873 35 550 Fragments arranged by size: 873 550 35 EaeI y'GGCC_r Cuts at: 0 137 350 831 974 1397 1458 Size: 137 213 481 143 423 61 Fragments arranged by size: 481 423 213 143 137 61 EarI CTCTTCn'nnn_ Cuts at: 0 184 439 1018 1458 Size: 184 255 579 440 Fragments arranged by size: 579 440 255 184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 313 1385 1458 Size: 119 194 1072 73 Fragments arranged by size: 1072 194 119 73 EcoNI CCTnn'n_nnAGG Cuts at: 0 631 1458 Size: 631 827 EcoO109I rG'GnC_Cy Cuts at: 0 104 194 398 422 584 812 1458 Size: 104 90 204 24 162 228 646 Fragments arranged by size: 646 228 204 162 104 90 24 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 486 1458 Size: 486 972 HaeII r_GCGC'y Cuts at: 0 495 846 1458 Size: 495 351 612 Fragments arranged by size: 612 495 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 161 193 1360 1392 1458 Size: 161 32 1167 32 66 Fragments arranged by size: 1167 161 66 32 32 HincII GTy'rAC Cuts at: 0 19 1458 Size: 19 1439 MscI TGG'CCA Cuts at: 0 139 352 976 1458 Size: 139 213 624 482 Fragments arranged by size: 624 482 213 139 MslI CAynn'nnrTG Cuts at: 0 328 346 901 1144 1255 1458 Size: 328 18 555 243 111 203 Fragments arranged by size: 555 328 243 203 111 18 MspA1I CmG'CkG Cuts at: 0 1211 1227 1458 Size: 1211 16 231 Fragments arranged by size: 1211 231 16 NaeI GCC'GGC Cuts at: 0 40 1458 Size: 40 1418 NcoI C'CATG_G Cuts at: 0 1166 1458 Size: 1166 292 NdeI CA'TA_TG Cuts at: 0 898 1458 Size: 898 560 NgoMIV G'CCGG_C Cuts at: 0 38 1458 Size: 38 1420 NspI r_CATG'y Cuts at: 0 1083 1143 1374 1458 Size: 1083 60 231 84 Fragments arranged by size: 1083 231 84 60 PasI CC'CwG_GG Cuts at: 0 190 204 426 1458 Size: 190 14 222 1032 Fragments arranged by size: 1032 222 190 14 PciI A'CATG_T Cuts at: 0 1079 1370 1458 Size: 1079 291 88 Fragments arranged by size: 1079 291 88 PflMI CCAn_nnn'nTGG Cuts at: 0 118 998 1335 1458 Size: 118 880 337 123 Fragments arranged by size: 880 337 123 118 PfoI T'CCnGG_A Cuts at: 0 290 1458 Size: 290 1168 PpuMI rG'GwC_Cy Cuts at: 0 104 398 422 1458 Size: 104 294 24 1036 Fragments arranged by size: 1036 294 104 24 PspOMI G'GGCC_C Cuts at: 0 200 583 1458 Size: 200 383 875 Fragments arranged by size: 875 383 200 PstI C_TGCA'G Cuts at: 0 229 1458 Size: 229 1229 PvuII CAG'CTG Cuts at: 0 1227 1458 Size: 1227 231 SanDI GG'GwC_CC Cuts at: 0 104 398 422 1458 Size: 104 294 24 1036 Fragments arranged by size: 1036 294 104 24 SfcI C'TryA_G Cuts at: 0 225 1458 Size: 225 1233 SmaI CCC'GGG Cuts at: 0 589 1093 1458 Size: 589 504 365 Fragments arranged by size: 589 504 365 StuI AGG'CCT Cuts at: 0 236 1159 1458 Size: 236 923 299 Fragments arranged by size: 923 299 236 StyI C'CwwG_G Cuts at: 0 152 231 1166 1458 Size: 152 79 935 292 Fragments arranged by size: 935 292 152 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 585 787 891 1458 Size: 585 202 104 567 Fragments arranged by size: 585 567 202 104 TatI w'GTAC_w Cuts at: 0 248 1373 1458 Size: 248 1125 85 Fragments arranged by size: 1125 248 85 Tth111I GACn'n_nGTC Cuts at: 0 760 1047 1458 Size: 760 287 411 Fragments arranged by size: 760 411 287 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 115 345 750 1095 1458 Size: 115 230 405 345 363 Fragments arranged by size: 405 363 345 230 115 XmaI C'CCGG_G Cuts at: 0 587 1091 1458 Size: 587 504 367 Fragments arranged by size: 587 504 367 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlwNI ApaI AvaI BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI BpmI BsaI BsaAI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SfcI SmaI StuI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstYI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI