(Circular) (Six-base) MAPSORT of: pFN21AE9460.seq Check: 2035 from: 1 to: 5662 pFN21AE9460 5662 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 3005 1937 Size: 804 264 4594 Fragments arranged by size: 4594 804 264 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2330 3725 5109 5377 1632 Size: 638 60 1395 1384 268 1917 Fragments arranged by size: 1917 1395 1384 638 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2145 1166 Size: 979 4683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 3405 4977 3405 Size: 1572 4090 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2516 2545 2572 2971 3197 3349 5458 1493 Size: 1023 29 27 399 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 399 226 152 29 27 AseI AT'TA_AT Cuts at: 160 1032 2166 4216 160 Size: 872 1134 2050 1606 Fragments arranged by size: 2050 1606 1134 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2987 1870 Size: 73 1044 4545 Fragments arranged by size: 4545 1044 73 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 2443 2983 4438 618 Size: 325 217 627 449 207 540 1455 1842 Fragments arranged by size: 1842 1455 627 540 449 325 217 207 BanII G_rGCy'C Cuts at: 1391 2981 1391 Size: 1590 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2336 928 Size: 971 437 4254 Fragments arranged by size: 4254 971 437 BbvCI CC'TCA_GC Cuts at: 2747 2747 Size: 5662 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 1903 Size: 34 1916 34 3678 Fragments arranged by size: 3678 1916 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 1548 Size: 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2807 4273 136 Size: 107 122 71 631 605 1135 1466 1525 Fragments arranged by size: 1525 1466 1135 631 605 122 107 71 BglII A'GATC_T Cuts at: 2041 2367 5657 2041 Size: 326 3290 2046 Fragments arranged by size: 3290 2046 326 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 3723 4879 1790 Size: 1933 1156 2573 Fragments arranged by size: 2573 1933 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 3393 4351 Size: 416 53 83 399 24 607 1369 958 Cuts at: 4351 442 Size: 1753 Fragments arranged by size: 1753 1369 958 607 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 4322 1239 Size: 827 2256 2579 Fragments arranged by size: 2579 2256 827 Bpu10I CC'TnA_GC Cuts at: 2747 2747 Size: 5662 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 4325 882 Size: 617 1413 1413 2219 Fragments arranged by size: 2219 1413 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 2058 3453 493 Size: 711 854 1395 2702 Fragments arranged by size: 2702 1395 854 711 BsaBI GATnn'nnATC Cuts at: 2462 5656 2462 Size: 3194 2468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5437 Fragments arranged by size: 5437 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5662 BseYI C'CCAG_C Cuts at: 4865 5361 4865 Size: 496 5166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5089 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3723 3808 4879 3723 Size: 85 1071 4506 Fragments arranged by size: 4506 1071 85 BsmI GAATG_Cn' Cuts at: 2876 3173 3266 2876 Size: 297 93 5272 Fragments arranged by size: 5272 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2981 3723 3808 4879 1391 Size: 399 1191 742 85 1071 2174 Fragments arranged by size: 2174 1191 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3017 844 Size: 2173 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2883 3549 1394 Size: 429 1060 666 3507 Fragments arranged by size: 3507 1060 666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4306 4493 1700 Size: 110 2496 187 2869 Fragments arranged by size: 2869 2496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstYI r'GATC_y Cuts at: 1972 2041 2367 2992 3359 3744 3761 5213 5299 Size: 69 326 625 367 385 17 1452 86 Cuts at: 5299 5311 5657 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 625 385 367 346 326 86 69 17 12 BstZ17I GTA'TAC Cuts at: 2742 2742 Size: 5662 BtgI C'CryG_G Cuts at: 513 1065 2691 513 Size: 552 1626 3484 Fragments arranged by size: 3484 1626 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 2260 2813 3178 3972 3992 2260 Size: 553 365 794 20 3930 Fragments arranged by size: 3930 794 553 365 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2509 2571 2966 2509 Size: 62 395 5205 Fragments arranged by size: 5205 395 62 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3109 3998 5627 Size: 54 1425 50 275 18 1279 889 1629 Cuts at: 5627 8 Size: 43 Fragments arranged by size: 1629 1425 1279 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2798 3592 1353 Size: 1445 794 3423 Fragments arranged by size: 3423 1445 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2270 2330 3725 4322 5109 5377 Size: 393 434 204 60 1395 597 787 268 Cuts at: 5377 1239 Size: 1524 Fragments arranged by size: 1524 1395 787 597 434 393 268 204 60 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 EcoRV GAT'ATC Cuts at: 2406 2406 Size: 5662 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 4809 1678 Size: 3131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2911 2943 4303 4335 4377 Size: 32 135 32 1339 32 1360 32 42 Cuts at: 4377 4409 1373 Size: 32 2626 Fragments arranged by size: 2626 1360 1339 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3006 3252 669 Size: 988 281 286 782 246 3079 Fragments arranged by size: 3079 988 782 286 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 2599 3261 2599 Size: 662 5000 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2065 4776 4960 1305 Size: 760 2711 184 2007 Fragments arranged by size: 2711 2007 760 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3840 Fragments arranged by size: 3840 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 3620 3979 4138 518 Size: 648 979 1475 359 159 2042 Fragments arranged by size: 2042 1475 979 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2340 2350 2361 3078 3755 4903 5148 1606 Size: 734 10 11 717 677 1148 245 2120 Fragments arranged by size: 2120 1148 734 717 677 245 11 10 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5110 NdeI CA'TA_TG Cuts at: 387 2552 2705 387 Size: 2165 153 3344 Fragments arranged by size: 3344 2165 153 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 3020 4565 1132 Size: 97 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 97 PacI TTA_AT'TAA Cuts at: 2166 2166 Size: 5662 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5662 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2340 2759 1613 Size: 727 419 4516 Fragments arranged by size: 4516 727 419 PsiI TTA'TAA Cuts at: 2037 2438 3232 2037 Size: 401 794 4467 Fragments arranged by size: 4467 794 401 PstI C_TGCA'G Cuts at: 830 3014 830 Size: 2184 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2350 2361 2350 Size: 11 5651 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2623 3010 4145 4826 5017 826 Size: 219 1578 387 1135 681 191 1471 Fragments arranged by size: 1578 1471 1135 681 387 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3766 4667 4929 820 Size: 197 926 1823 901 262 1553 Fragments arranged by size: 1823 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 2565 3586 5 Size: 47 2513 1021 2081 Fragments arranged by size: 2513 2081 1021 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2240 2922 3101 513 Size: 552 1175 682 179 3074 Fragments arranged by size: 3074 1175 682 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 3689 3706 3859 4044 4489 325 Size: 1255 639 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 3908 5620 Size: 274 80 33 51 1443 879 1052 1712 Cuts at: 5620 96 Size: 138 Fragments arranged by size: 1712 1443 1052 879 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 5622 Size: 32 5630 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5662 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 2255 3791 2255 Size: 1536 4126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI