################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AccI AccII AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FseI FspI HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1225 GACGTC GACGT^C Acc65I 2017 GGTACC G^GTACC AcyI 1225 GACGTC G[GA]^CG[CT]C Aor51HI 301 AGCGCT AGC^GCT Asp700I 1964 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2017 GGTACC G^GTACC AspI 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 1440 TGATCA T^GATCA BglII 1662 AGATCT A^GATCT BlpI 1813 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1813 GCTCAGC GC^T[ACGT]AGC BsaHI 1225 GACGTC G[GA]^CG[CT]C BsmI 843 GCATTC GAATGC(1/-1) Bsp1407I 895 TGTACA T^GTACA BspLU11I 1905 ACATGT A^CATGT BsrDI 939 GCAATG GCAATG(2/0) BsrFI 2276 ACCGGC [GA]^CCGG[CT] BsrGI 895 TGTACA T^GTACA BstEII 1279 GGTCACC G^GT[ACGT]ACC BstPI 1279 GGTCACC G^GT[ACGT]ACC CelII 1813 GCTCAGC GC^T[ACGT]AGC CfoI 302 GCGC GCG^C Cfr10I 2276 ACCGGC [GA]^CCGG[CT] DrdI 363 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 301 AGCGCT AGC^GCT EcoO65I 1279 GGTCACC G^GT[ACGT]ACC EcoRV 1416 GATATC GAT^ATC FbaI 1440 TGATCA T^GATCA HaeII 301 AGCGCT [GA]GCGC^[CT] HgaI 977 GACGC GACGC(5/10) HhaI 302 GCGC GCG^C Hin1I 1225 GACGTC G[GA]^CG[CT]C HinP1I 302 GCGC G^CGC HincII 790 GTCAAC GT[CT]^[GA]AC HindII 790 GTCAAC GT[CT]^[GA]AC HindIII 1011 AAGCTT A^AGCTT KpnI 2017 GGTACC GGTAC^C MseI 772 TTAA T^TAA NdeI 1352 CATATG CA^TATG NspI 1905 ACATGT [GA]CATG^[CT] PflFI 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 2306 GCTCTTC GCTCTTC(1/4) SbfI 1113 CCTGCAGG CCTGCA^GG SfiI 730 GGCCTGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1319 CCCGGG CCC^GGG Sse8387I 1113 CCTGCAGG CCTGCA^GG SspBI 895 TGTACA T^GTACA SspI 989 AATATT AAT^ATT TaqI 628 TCGA T^CGA Tru9I 772 TTAA T^TAA Tth111I 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 628 TCGA T^CGA XcmI 1017 CCATACCCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1319 CCCGGG C^CCGGG XmaI 1319 CCCGGG C^CCGGG XmnI 1964 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 614 ACGTGT A^C[GA][CT]GT 1905 ACATGT Alw44I 1170 GTGCAC G^TGCAC 1332 GTGCAC AocI 1678 CCTGAGG CC^T[ACGT]AGG 2192 CCTCAGG ApaLI 1170 GTGCAC G^TGCAC 1332 GTGCAC BanI 715 GGTGCC G^G[CT][GA]CC 2017 GGTACC BbsI 1964 GAAGAC GAAGAC(2/6) 2211 GTCTTC BfaI 2063 CTAG C^TAG 2318 CTAG BglI 718 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 731 GCCTGACTGGC BpuAI 1964 GAAGAC GAAGAC(2/6) 2211 GTCTTC BsaI 63 GAGACC GGTCTC(1/5) 1505 GAGACC BspHI 72 TCATGA T^CATGA 539 TCATGA BspMI 485 ACCTGC ACCTGC(4/8) 1203 ACCTGC Bsu36I 1678 CCTGAGG CC^T[ACGT]AGG 2192 CCTCAGG DsaI 1070 CCATGG C^C[GA][CT]GG 1471 CCATGG Eco81I 1678 CCTGAGG CC^T[ACGT]AGG 2192 CCTCAGG EcoNI 1182 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1761 CCTCTCCAAGG MaeI 2063 CTAG C^TAG 2318 CTAG NcoI 1070 CCATGG C^CATGG 1471 CCATGG PflMI 48 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1248 CCACACCCTGG PpuMI 388 GGGACCT [GA]G^G[AT]CC[CT] 455 GGGTCCC RcaI 72 TCATGA T^CATGA 539 TCATGA SacI 339 GAGCTC GAGCT^C 2304 GAGCTC SexAI 857 ACCTGGT A^CC[AT]GGT 2013 ACCAGGT SmlI 1366 CTTGAG C^T[CT][GA]AG 2003 CTCAAG Van91I 48 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1248 CCACACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII