(Circular) (Six-base) MAPSORT of: pFN21AE9432.seq Check: 773 from: 1 to: 7147 pFN21AE9432 7147 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3217 3217 Size: 7147 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3234 4957 278 Size: 53 83 186 936 1698 1723 2468 Fragments arranged by size: 2468 1723 1698 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4490 1937 Size: 2553 4594 Acc65I G'GTAC_C Cuts at: 4022 4468 4022 Size: 446 6701 AclI AA'CG_TT Cuts at: 1563 5274 5647 1563 Size: 3711 373 3063 Fragments arranged by size: 3711 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2960 3327 3635 3730 3984 5210 6594 6862 Size: 1328 367 308 95 254 1226 1384 268 Cuts at: 6862 1632 Size: 1917 Fragments arranged by size: 1917 1384 1328 1226 367 308 268 254 95 AfeI AGC'GCT Cuts at: 2308 2308 Size: 7147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6950 AflIII A'CryG_T Cuts at: 1203 2619 3910 6046 7023 1203 Size: 1416 1291 2136 977 1327 Fragments arranged by size: 2136 1416 1327 1291 977 AgeI A'CCGG_T Cuts at: 5978 5978 Size: 7147 AhdI GACnn_n'nnGTC Cuts at: 5876 5876 Size: 7147 AleI CACnn'nnGTG Cuts at: 1166 2649 4442 1166 Size: 1483 1793 3871 Fragments arranged by size: 3871 1793 1483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2720 1184 Size: 1536 5611 AlwNI CAG_nnn'CTG Cuts at: 2059 2500 4346 4890 6462 2059 Size: 441 1846 544 1572 2744 Fragments arranged by size: 2744 1846 1572 544 441 ApaI G_GGCC'C Cuts at: 3351 3683 3829 4120 4396 3351 Size: 332 146 291 276 6102 Fragments arranged by size: 6102 332 291 276 146 ApaLI G'TGCA_C Cuts at: 3175 3337 5204 6360 3175 Size: 162 1867 1156 3962 Fragments arranged by size: 3962 1867 1156 162 ApoI r'AATT_y Cuts at: 1493 4456 4682 4834 6943 1493 Size: 2963 226 152 2109 1697 Fragments arranged by size: 2963 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5701 160 Size: 872 4669 1606 Fragments arranged by size: 4669 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 AvaI C'yCGr_G Cuts at: 1870 1943 3055 3324 4058 4472 1870 Size: 73 1112 269 734 414 4545 Fragments arranged by size: 4545 1112 734 414 269 73 BamHI G'GATC_C Cuts at: 4477 4844 4477 Size: 367 6780 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2720 4022 4468 5923 618 Size: 325 217 627 933 1302 446 1455 1842 Fragments arranged by size: 1842 1455 1302 933 627 446 325 217 BanII G_rGCy'C Cuts at: 1391 2348 3351 3606 3683 3829 4059 4120 4313 Size: 957 1003 255 77 146 230 61 193 Cuts at: 4313 4396 4466 1391 Size: 83 70 4072 Fragments arranged by size: 4072 1003 957 255 230 193 146 83 77 70 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3976 4209 928 Size: 971 2077 233 3866 Fragments arranged by size: 3866 2077 971 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5338 5372 1903 Size: 34 3401 34 3678 Fragments arranged by size: 3678 3401 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5039 6249 5039 Size: 1210 5937 BclI T'GATC_A Cuts at: 1548 3445 1548 Size: 1897 5250 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 2742 5758 Size: 107 122 71 631 605 1057 13 3016 Cuts at: 5758 136 Size: 1525 Fragments arranged by size: 3016 1525 1057 631 605 122 107 71 13 BglII A'GATC_T Cuts at: 3667 7142 3667 Size: 3475 3672 BlpI GC'TnA_GC Cuts at: 3819 4564 3819 Size: 745 6402 Bme1580I G_kGCm'C Cuts at: 1790 3179 3341 3351 3683 3829 3898 4120 4197 Size: 1389 162 10 332 146 69 222 77 Cuts at: 4197 4396 5208 6364 1790 Size: 199 812 1156 2573 Fragments arranged by size: 2573 1389 1156 812 332 222 199 162 146 77 69 10 BmgBI CAC'GTC Cuts at: 1318 2550 2620 1318 Size: 1232 70 5845 Fragments arranged by size: 5845 1232 70 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3022 3328 Size: 416 53 83 399 24 874 731 306 Cuts at: 3328 4184 4371 4878 5836 442 Size: 856 187 507 958 1753 Fragments arranged by size: 1753 958 874 856 731 507 416 399 306 187 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3088 3088 Size: 7147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2564 2904 3376 3608 4049 5807 1239 Size: 1325 340 472 232 441 1758 2579 Fragments arranged by size: 2579 1758 1325 472 441 340 232 Bpu10I CC'TnA_GC Cuts at: 3270 4353 3270 Size: 1083 6064 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3392 3993 5272 6137 6435 3392 Size: 601 1279 865 298 4104 Fragments arranged by size: 4104 1279 865 601 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2062 3504 5810 882 Size: 617 563 1442 2306 2219 Fragments arranged by size: 2306 2219 1442 617 563 BsaAI yAC'GTr Cuts at: 493 1204 4938 493 Size: 711 3734 2702 Fragments arranged by size: 3734 2702 711 BsaBI GATnn'nnATC Cuts at: 7141 7141 Size: 7147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3231 4954 5336 5971 Size: 53 83 186 936 1698 1723 382 635 Cuts at: 5971 275 Size: 1451 Fragments arranged by size: 1723 1698 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5580 5978 6252 6399 6810 5580 Size: 398 274 147 411 5917 Fragments arranged by size: 5917 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3501 3531 1178 Size: 30 165 30 2098 30 4794 Fragments arranged by size: 4794 2098 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3053 3056 3077 3343 3428 3522 3614 3817 Size: 1867 3 21 266 85 94 92 203 Cuts at: 3817 1186 Size: 4516 Fragments arranged by size: 4516 1867 266 203 94 92 85 21 3 BseYI C'CCAG_C Cuts at: 2500 2816 2934 3530 3778 3932 6350 6846 2500 Size: 316 118 596 248 154 2418 496 2801 Fragments arranged by size: 2801 2418 596 496 316 248 154 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2611 3834 3935 1163 Size: 573 875 1223 101 4375 Fragments arranged by size: 4375 1223 875 573 101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4597 5358 6386 1540 Size: 275 186 2596 761 1028 2301 Fragments arranged by size: 2596 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2348 3179 3341 4313 5208 5293 6364 2348 Size: 831 162 972 895 85 1071 3131 Fragments arranged by size: 3131 1071 972 895 831 162 85 BsmI GAATG_Cn' Cuts at: 2848 4658 4751 2848 Size: 1810 93 5244 Fragments arranged by size: 5244 1810 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2348 3179 3341 3351 3606 3683 3829 Size: 399 558 831 162 10 255 77 146 Cuts at: 3829 3898 4059 4120 4197 4313 4396 4466 5208 Size: 69 161 61 77 116 83 70 742 Cuts at: 5208 5293 6364 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 831 742 558 399 255 162 161 146 116 85 83 77 77 70 69 61 10 BspEI T'CCGG_A Cuts at: 6810 6810 Size: 7147 BspHI T'CATG_A Cuts at: 2077 2544 5036 6766 2077 Size: 467 2492 1730 2458 Fragments arranged by size: 2492 2458 1730 467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 3217 4502 844 Size: 1655 718 1285 3489 Fragments arranged by size: 3489 1655 1285 718 BsrBI CCG'CTC Cuts at: 1394 1823 3613 3831 4398 5034 1394 Size: 429 1790 218 567 636 3507 Fragments arranged by size: 3507 1790 636 567 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 2951 5642 5816 65 Size: 1183 1703 2691 174 1396 Fragments arranged by size: 2691 1703 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4281 5791 5978 1700 Size: 110 2471 1510 187 2869 Fragments arranged by size: 2869 2471 1510 187 110 BsrGI T'GTAC_A Cuts at: 96 2900 96 Size: 2804 4343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7147 BssSI C'ACGA_G Cuts at: 5207 6219 5207 Size: 1012 6135 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7147 BstBI TT'CG_AA Cuts at: 5951 5951 Size: 7147 BstEII G'GTnAC_C Cuts at: 1184 3284 1184 Size: 2100 5047 BstXI CCAn_nnnn'nTGG Cuts at: 2788 3778 4103 4444 2788 Size: 990 325 341 5491 Fragments arranged by size: 5491 990 341 325 BstYI r'GATC_y Cuts at: 1972 2889 3565 3667 4003 4477 4844 5229 5246 Size: 917 676 102 336 474 367 385 17 Cuts at: 5246 6698 6784 6796 7142 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 917 676 474 385 367 346 336 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3684 4198 3684 Size: 514 6633 BtgI C'CryG_G Cuts at: 513 1065 2004 3075 3476 513 Size: 552 939 1071 401 4184 Fragments arranged by size: 4184 1071 939 552 401 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6408 Fragments arranged by size: 6408 683 56 BtsI GCAGTG_nn' Cuts at: 2221 4663 5457 5477 2221 Size: 2442 794 20 3891 Fragments arranged by size: 3891 2442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7147 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7112 DraI TTT'AAA Cuts at: 4451 4451 Size: 7147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2374 6154 809 Size: 507 1058 3780 1802 Fragments arranged by size: 3780 1802 1058 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2743 2988 Size: 54 1425 50 275 18 220 693 245 Cuts at: 2988 4111 4594 5483 7112 8 Size: 1123 483 889 1629 43 Fragments arranged by size: 1629 1425 1123 889 693 483 275 245 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4594 1537 Size: 275 2782 4090 Fragments arranged by size: 4090 2782 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2137 2955 3220 4318 5077 1353 Size: 784 818 265 1098 759 3423 Fragments arranged by size: 3423 1098 818 784 759 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5734 6108 6254 387 Size: 1064 138 4145 374 146 1280 Fragments arranged by size: 4145 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2346 4311 2346 Size: 1965 5182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2904 2960 3327 3376 3608 3635 Size: 393 932 340 56 367 49 232 27 Cuts at: 3635 3730 3984 4049 5210 5807 6594 6862 1239 Size: 95 254 65 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 932 787 597 393 367 340 268 254 232 95 65 56 49 27 EcoNI CCTnn'n_nnAGG Cuts at: 3191 3770 3191 Size: 579 6568 EcoO109I rG'GnC_Cy Cuts at: 2394 2461 3275 3347 3348 3377 3680 4117 2394 Size: 67 814 72 1 29 303 437 5424 Fragments arranged by size: 5424 814 437 303 72 67 29 1 EcoRI G'AATT_C Cuts at: 4456 4834 4456 Size: 378 6769 EcoRV GAT'ATC Cuts at: 3423 3423 Size: 7147 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3993 3993 Size: 7147 FspI TGC'GCA Cuts at: 5653 5653 Size: 7147 HaeII r_GCGC'y Cuts at: 1678 2310 6294 1678 Size: 632 3984 2531 Fragments arranged by size: 3984 2531 632 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2239 2271 2466 2498 3407 Size: 32 135 32 667 32 195 32 909 Cuts at: 3407 3439 3501 3533 3602 3634 4229 4261 5788 Size: 32 62 32 69 32 595 32 1527 Cuts at: 5788 5820 5862 5894 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 909 667 595 195 135 69 62 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 4491 4737 669 Size: 988 281 859 1694 246 3079 Fragments arranged by size: 3079 1694 988 859 281 246 HindIII A'AGCT_T Cuts at: 748 3016 748 Size: 2268 4879 HpaI GTT'AAC Cuts at: 4737 4737 Size: 7147 KpnI G_GTAC'C Cuts at: 4026 4472 4026 Size: 446 6701 MfeI C'AATT_G Cuts at: 4746 4746 Size: 7147 MluI A'CGCG_T Cuts at: 7023 7023 Size: 7147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6261 6445 1305 Size: 4956 184 2007 Fragments arranged by size: 4956 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2052 2745 2990 10 Size: 54 1425 343 220 693 245 4167 Fragments arranged by size: 4167 1425 693 343 245 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 2649 2786 4442 5105 5464 5623 518 Size: 648 1483 137 1656 663 359 159 2042 Fragments arranged by size: 2042 1656 1483 663 648 359 159 137 MspA1I CmG'CkG Cuts at: 1606 2178 2938 3170 3266 3823 4180 4275 4563 Size: 572 760 232 96 557 357 95 288 Cuts at: 4563 5240 6388 6633 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 760 677 572 557 357 288 245 232 96 95 NcoI C'CATG_G Cuts at: 513 1065 2004 3075 3476 513 Size: 552 939 1071 401 4184 Fragments arranged by size: 4184 1071 939 552 401 NdeI CA'TA_TG Cuts at: 387 3358 387 Size: 2971 4176 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7147 NotI GC'GGCC_GC Cuts at: 4594 4594 Size: 7147 NspI r_CATG'y Cuts at: 1132 1229 3914 4505 6050 1132 Size: 97 2685 591 1545 2229 Fragments arranged by size: 2685 2229 1545 591 97 PasI CC'CwG_GG Cuts at: 1348 3404 1348 Size: 2056 5091 PciI A'CATG_T Cuts at: 3910 6046 3910 Size: 2136 5011 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 3259 1108 Size: 951 1200 4996 Fragments arranged by size: 4996 1200 951 PfoI T'CCnGG_A Cuts at: 2463 3980 2463 Size: 1517 5630 PmeI GTTT'AAAC Cuts at: 4451 4451 Size: 7147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5216 5248 6729 6761 5216 Size: 32 1481 32 5602 Fragments arranged by size: 5602 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2394 2461 2394 Size: 67 7080 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7147 PsiI TTA'TAA Cuts at: 4717 4717 Size: 7147 PspOMI G'GGCC_C Cuts at: 3347 3679 3825 4116 4392 3347 Size: 332 146 291 276 6102 Fragments arranged by size: 6102 332 291 276 146 PstI C_TGCA'G Cuts at: 830 2228 2496 3123 4499 830 Size: 1398 268 627 1376 3478 Fragments arranged by size: 3478 1398 1376 627 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7147 PvuII CAG'CTG Cuts at: 2178 2938 3170 3266 4180 2178 Size: 760 232 96 914 5145 Fragments arranged by size: 5145 914 760 232 96 SacI G_AGCT'C Cuts at: 2348 4313 2348 Size: 1965 5182 SalI G'TCGA_C Cuts at: 1936 4489 1936 Size: 2553 4594 SanDI GG'GwC_CC Cuts at: 2461 2461 Size: 7147 SapI GCTCTTCn'nnn_ Cuts at: 4318 4318 Size: 7147 SbfI CC_TGCA'GG Cuts at: 3123 4499 3123 Size: 1376 5771 ScaI AGT'ACT Cuts at: 5395 7107 5395 Size: 1712 5435 SexAI A'CCwGG_T Cuts at: 2862 4018 2862 Size: 1156 5991 SfcI C'TryA_G Cuts at: 826 1045 2224 2492 3119 4495 5630 6311 6502 Size: 219 1179 268 627 1376 1135 681 191 Cuts at: 6502 826 Size: 1471 Fragments arranged by size: 1471 1376 1179 1135 681 627 268 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2742 2742 Size: 7147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7147 SmaI CCC'GGG Cuts at: 1872 3326 4474 1872 Size: 1454 1148 4545 Fragments arranged by size: 4545 1454 1148 SmlI C'TyrA_G Cuts at: 820 1017 1943 3371 4008 5251 6152 6414 820 Size: 197 926 1428 637 1243 901 262 1553 Fragments arranged by size: 1553 1428 1243 926 901 637 262 197 SnaBI TAC'GTA Cuts at: 493 4938 493 Size: 4445 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7147 SphI G_CATG'C Cuts at: 1132 4505 1132 Size: 3373 3774 SspI AAT'ATT Cuts at: 5 52 2996 5071 5 Size: 47 2944 2075 2081 Fragments arranged by size: 2944 2081 2075 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7147 StyI C'CwwG_G Cuts at: 513 1065 2004 3075 3476 3771 4586 513 Size: 552 939 1071 401 295 815 3074 Fragments arranged by size: 3074 1071 939 815 552 401 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2542 2657 3238 5174 5191 5344 5529 Size: 1255 962 115 581 1936 17 153 185 Cuts at: 5529 5974 325 Size: 445 1498 Fragments arranged by size: 1936 1498 1255 962 581 445 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2806 2900 5393 Size: 274 80 33 51 1443 829 94 2493 Cuts at: 5393 7105 96 Size: 1712 138 Fragments arranged by size: 2493 1712 1443 829 274 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3320 3352 3721 3753 4373 4405 7107 7139 3320 Size: 32 369 32 620 32 2702 32 3328 Fragments arranged by size: 3328 2702 620 369 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3515 1316 Size: 2199 4948 XbaI T'CTAG_A Cuts at: 4483 4483 Size: 7147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3029 3029 Size: 7147 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7147 XmaI C'CCGG_G Cuts at: 1870 3324 4472 1870 Size: 1454 1148 4545 Fragments arranged by size: 4545 1454 1148 XmnI GAAnn'nnTTC Cuts at: 3973 5276 3973 Size: 1303 5844 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3232 4955 276 Size: 53 83 186 936 1698 1723 2468 Fragments arranged by size: 2468 1723 1698 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfoI SrfI SwaI