(Linear) (Six-base) MAPSORT of: KIEE9432.seq Check: 2182 from: 1 to: 2442 KIEE9432.orf With 182 enzymes: SgfI * December 27, 2011 16:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1212 2442 Size: 1212 1230 AatII G_ACGT'C Cuts at: 0 1229 2442 Size: 1229 1213 Acc65I G'GTAC_C Cuts at: 0 2017 2442 Size: 2017 425 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 955 1322 1630 1725 1979 2442 Size: 955 367 308 95 254 463 Fragments arranged by size: 955 463 367 308 254 95 AfeI AGC'GCT Cuts at: 0 303 2442 Size: 303 2139 AflIII A'CryG_T Cuts at: 0 614 1905 2442 Size: 614 1291 537 Fragments arranged by size: 1291 614 537 AleI CACnn'nnGTG Cuts at: 0 644 2437 2442 Size: 644 1793 5 Fragments arranged by size: 1793 644 5 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 715 2442 Size: 715 1727 AlwNI CAG_nnn'CTG Cuts at: 0 54 495 2341 2442 Size: 54 441 1846 101 Fragments arranged by size: 1846 441 101 54 ApaI G_GGCC'C Cuts at: 0 1346 1678 1824 2115 2391 2442 Size: 1346 332 146 291 276 51 Fragments arranged by size: 1346 332 291 276 146 51 ApaLI G'TGCA_C Cuts at: 0 1170 1332 2442 Size: 1170 162 1110 Fragments arranged by size: 1170 1110 162 AvaI C'yCGr_G Cuts at: 0 1050 1319 2053 2442 Size: 1050 269 734 389 Fragments arranged by size: 1050 734 389 269 BanI G'GyrC_C Cuts at: 0 715 2017 2442 Size: 715 1302 425 Fragments arranged by size: 1302 715 425 BanII G_rGCy'C Cuts at: 0 343 1346 1601 1678 1824 2054 2115 2308 Size: 343 1003 255 77 146 230 61 193 Cuts at: 2308 2391 2442 Size: 83 51 Fragments arranged by size: 1003 343 255 230 193 146 83 77 61 51 BbsI GAAGACnn'nnnn_ Cuts at: 0 1971 2204 2442 Size: 1971 233 238 Fragments arranged by size: 1971 238 233 BclI T'GATC_A Cuts at: 0 1440 2442 Size: 1440 1002 BglI GCCn_nnn'nGGC Cuts at: 0 724 737 2442 Size: 724 13 1705 Fragments arranged by size: 1705 724 13 BglII A'GATC_T Cuts at: 0 1662 2442 Size: 1662 780 BlpI GC'TnA_GC Cuts at: 0 1814 2442 Size: 1814 628 Bme1580I G_kGCm'C Cuts at: 0 1174 1336 1346 1678 1824 1893 2115 2192 Size: 1174 162 10 332 146 69 222 77 Cuts at: 2192 2391 2442 Size: 199 51 Fragments arranged by size: 1174 332 222 199 162 146 77 69 51 10 BmgBI CAC'GTC Cuts at: 0 545 615 2442 Size: 545 70 1827 Fragments arranged by size: 1827 545 70 BmrI ACTGGGnnnn_n' Cuts at: 0 286 1017 1323 2179 2366 2442 Size: 286 731 306 856 187 76 Fragments arranged by size: 856 731 306 286 187 76 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1083 2442 Size: 1083 1359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 899 1371 1603 2044 2442 Size: 559 340 472 232 441 398 Fragments arranged by size: 559 472 441 398 340 232 Bpu10I CC'TnA_GC Cuts at: 0 1265 2348 2442 Size: 1265 1083 94 Fragments arranged by size: 1265 1083 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1387 1988 2442 Size: 1387 601 454 Fragments arranged by size: 1387 601 454 BsaI GGTCTCn'nnnn_ Cuts at: 0 57 1499 2442 Size: 57 1442 943 Fragments arranged by size: 1442 943 57 BsaHI Gr'CG_yC Cuts at: 0 1226 2442 Size: 1226 1216 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1496 1526 2442 Size: 1496 30 916 Fragments arranged by size: 1496 916 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1048 1051 1072 1338 1423 1517 1609 1812 Size: 1048 3 21 266 85 94 92 203 Cuts at: 1812 2442 Size: 630 Fragments arranged by size: 1048 630 266 203 94 92 85 21 3 BseYI C'CCAG_C Cuts at: 0 495 811 929 1525 1773 1927 2442 Size: 495 316 118 596 248 154 515 Fragments arranged by size: 596 515 495 316 248 154 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 606 1829 1930 2442 Size: 606 1223 101 512 Fragments arranged by size: 1223 606 512 101 BsiHKAI G_wGCw'C Cuts at: 0 343 1174 1336 2308 2442 Size: 343 831 162 972 134 Fragments arranged by size: 972 831 343 162 134 BsmI GAATG_Cn' Cuts at: 0 843 2442 Size: 843 1599 Bsp1286I G_dGCh'C Cuts at: 0 343 1174 1336 1346 1601 1678 1824 1893 Size: 343 831 162 10 255 77 146 69 Cuts at: 1893 2054 2115 2192 2308 2391 2442 Size: 161 61 77 116 83 51 Fragments arranged by size: 831 343 255 162 161 146 116 83 77 77 69 61 51 10 BspHI T'CATG_A Cuts at: 0 72 539 2442 Size: 72 467 1903 Fragments arranged by size: 1903 467 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 494 1212 2442 Size: 494 718 1230 Fragments arranged by size: 1230 718 494 BsrBI CCG'CTC Cuts at: 0 1608 1826 2393 2442 Size: 1608 218 567 49 Fragments arranged by size: 1608 567 218 49 BsrDI GCAATG_nn' Cuts at: 0 946 2442 Size: 946 1496 BsrFI r'CCGG_y Cuts at: 0 2276 2442 Size: 2276 166 BsrGI T'GTAC_A Cuts at: 0 895 2442 Size: 895 1547 BstEII G'GTnAC_C Cuts at: 0 1279 2442 Size: 1279 1163 BstXI CCAn_nnnn'nTGG Cuts at: 0 783 1773 2098 2442 Size: 783 990 325 344 Fragments arranged by size: 990 783 344 325 BstYI r'GATC_y Cuts at: 0 884 1560 1662 1998 2442 Size: 884 676 102 336 444 Fragments arranged by size: 884 676 444 336 102 Bsu36I CC'TnA_GG Cuts at: 0 1679 2193 2442 Size: 1679 514 249 Fragments arranged by size: 1679 514 249 BtgI C'CryG_G Cuts at: 0 1070 1471 2442 Size: 1070 401 971 Fragments arranged by size: 1070 971 401 BtsI GCAGTG_nn' Cuts at: 0 216 2442 Size: 216 2226 DrdI GACnn_nn'nnGTC Cuts at: 0 369 2442 Size: 369 2073 EaeI y'GGCC_r Cuts at: 0 45 738 983 2106 2442 Size: 45 693 245 1123 336 Fragments arranged by size: 1123 693 336 245 45 EarI CTCTTCn'nnn_ Cuts at: 0 132 950 1215 2313 2442 Size: 132 818 265 1098 129 Fragments arranged by size: 1098 818 265 132 129 EcoICRI GAG'CTC Cuts at: 0 341 2306 2442 Size: 341 1965 136 Fragments arranged by size: 1965 341 136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 899 955 1322 1371 1603 1630 1725 Size: 559 340 56 367 49 232 27 95 Cuts at: 1725 1979 2044 2442 Size: 254 65 398 Fragments arranged by size: 559 398 367 340 254 232 95 65 56 49 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 1186 1765 2442 Size: 1186 579 677 Fragments arranged by size: 1186 677 579 EcoO109I rG'GnC_Cy Cuts at: 0 389 456 1270 1342 1343 1372 1675 2112 Size: 389 67 814 72 1 29 303 437 Cuts at: 2112 2442 Size: 330 Fragments arranged by size: 814 437 389 330 303 72 67 29 1 EcoRV GAT'ATC Cuts at: 0 1418 2442 Size: 1418 1024 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1988 2442 Size: 1988 454 HaeII r_GCGC'y Cuts at: 0 305 2442 Size: 305 2137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 234 266 461 493 1402 1434 1496 1528 Size: 234 32 195 32 909 32 62 32 Cuts at: 1528 1597 1629 2224 2256 2442 Size: 69 32 595 32 186 Fragments arranged by size: 909 595 234 195 186 69 62 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 792 2442 Size: 792 1650 HindIII A'AGCT_T Cuts at: 0 1011 2442 Size: 1011 1431 KpnI G_GTAC'C Cuts at: 0 2021 2442 Size: 2021 421 MscI TGG'CCA Cuts at: 0 47 740 985 2442 Size: 47 693 245 1457 Fragments arranged by size: 1457 693 245 47 MslI CAynn'nnrTG Cuts at: 0 644 781 2437 2442 Size: 644 137 1656 5 Fragments arranged by size: 1656 644 137 5 MspA1I CmG'CkG Cuts at: 0 173 933 1165 1261 1818 2175 2270 2442 Size: 173 760 232 96 557 357 95 172 Fragments arranged by size: 760 557 357 232 173 172 96 95 NcoI C'CATG_G Cuts at: 0 1070 1471 2442 Size: 1070 401 971 Fragments arranged by size: 1070 971 401 NdeI CA'TA_TG Cuts at: 0 1353 2442 Size: 1353 1089 NspI r_CATG'y Cuts at: 0 1909 2442 Size: 1909 533 PasI CC'CwG_GG Cuts at: 0 1399 2442 Size: 1399 1043 PciI A'CATG_T Cuts at: 0 1905 2442 Size: 1905 537 PflMI CCAn_nnn'nTGG Cuts at: 0 54 1254 2442 Size: 54 1200 1188 Fragments arranged by size: 1200 1188 54 PfoI T'CCnGG_A Cuts at: 0 458 1975 2442 Size: 458 1517 467 Fragments arranged by size: 1517 467 458 PpuMI rG'GwC_Cy Cuts at: 0 389 456 2442 Size: 389 67 1986 Fragments arranged by size: 1986 389 67 PspOMI G'GGCC_C Cuts at: 0 1342 1674 1820 2111 2387 2442 Size: 1342 332 146 291 276 55 Fragments arranged by size: 1342 332 291 276 146 55 PstI C_TGCA'G Cuts at: 0 223 491 1118 2442 Size: 223 268 627 1324 Fragments arranged by size: 1324 627 268 223 PvuII CAG'CTG Cuts at: 0 173 933 1165 1261 2175 2442 Size: 173 760 232 96 914 267 Fragments arranged by size: 914 760 267 232 173 96 SacI G_AGCT'C Cuts at: 0 343 2308 2442 Size: 343 1965 134 Fragments arranged by size: 1965 343 134 SanDI GG'GwC_CC Cuts at: 0 456 2442 Size: 456 1986 SapI GCTCTTCn'nnn_ Cuts at: 0 2313 2442 Size: 2313 129 SbfI CC_TGCA'GG Cuts at: 0 1118 2442 Size: 1118 1324 SexAI A'CCwGG_T Cuts at: 0 857 2013 2442 Size: 857 1156 429 Fragments arranged by size: 1156 857 429 SfcI C'TryA_G Cuts at: 0 219 487 1114 2442 Size: 219 268 627 1328 Fragments arranged by size: 1328 627 268 219 SfiI GGCCn_nnn'nGGCC Cuts at: 0 737 2442 Size: 737 1705 SmaI CCC'GGG Cuts at: 0 1321 2442 Size: 1321 1121 SmlI C'TyrA_G Cuts at: 0 1366 2003 2442 Size: 1366 637 439 Fragments arranged by size: 1366 637 439 SspI AAT'ATT Cuts at: 0 991 2442 Size: 991 1451 StyI C'CwwG_G Cuts at: 0 1070 1471 1766 2442 Size: 1070 401 295 676 Fragments arranged by size: 1070 676 401 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 537 652 1233 2442 Size: 537 115 581 1209 Fragments arranged by size: 1209 581 537 115 TatI w'GTAC_w Cuts at: 0 801 895 2442 Size: 801 94 1547 Fragments arranged by size: 1547 801 94 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1315 1347 1716 1748 2368 2400 2442 Size: 1315 32 369 32 620 32 42 Fragments arranged by size: 1315 620 369 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1510 2442 Size: 1510 932 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1024 2442 Size: 1024 1418 XmaI C'CCGG_G Cuts at: 0 1319 2442 Size: 1319 1123 XmnI GAAnn'nnTTC Cuts at: 0 1968 2442 Size: 1968 474 ZraI GAC'GTC Cuts at: 0 1227 2442 Size: 1227 1215 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI MscI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SmaI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI AclI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI FseI FspI FspAI HpaI KasI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI XbaI XhoI