(Circular) (Six-base) MAPSORT of: pFN21AA1250.seq Check: 5450 from: 1 to: 10018 pFN21AA1250 10018 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5330 5330 Size: 10018 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7828 278 Size: 53 83 186 936 6292 2468 Fragments arranged by size: 6292 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6212 7361 1937 Size: 4275 1149 4594 Fragments arranged by size: 4594 4275 1149 Acc65I G'GTAC_C Cuts at: 7339 7339 Size: 10018 AclI AA'CG_TT Cuts at: 1563 7219 8145 8518 1563 Size: 5656 926 373 3063 Fragments arranged by size: 5656 3063 926 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3951 4343 4563 4992 5789 5880 5971 6456 Size: 2319 392 220 429 797 91 91 485 Cuts at: 6456 7014 8081 9465 9733 1632 Size: 558 1067 1384 268 1917 Fragments arranged by size: 2319 1917 1384 1067 797 558 485 429 392 268 220 91 91 AfeI AGC'GCT Cuts at: 2865 2865 Size: 10018 AflII C'TTAA_G Cuts at: 820 1017 6530 820 Size: 197 5513 4308 Fragments arranged by size: 5513 4308 197 AflIII A'CryG_T Cuts at: 1203 3965 8917 9894 1203 Size: 2762 4952 977 1327 Fragments arranged by size: 4952 2762 1327 977 AgeI A'CCGG_T Cuts at: 8849 8849 Size: 10018 AhdI GACnn_n'nnGTC Cuts at: 8747 8747 Size: 10018 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2499 1184 Size: 1315 8703 AlwNI CAG_nnn'CTG Cuts at: 2767 4366 7761 9333 2767 Size: 1599 3395 1572 3452 Fragments arranged by size: 3452 3395 1599 1572 ApaI G_GGCC'C Cuts at: 5334 5396 6206 5334 Size: 62 810 9146 Fragments arranged by size: 9146 810 62 ApaLI G'TGCA_C Cuts at: 2255 8075 9231 2255 Size: 5820 1156 3042 Fragments arranged by size: 5820 3042 1156 ApoI r'AATT_y Cuts at: 1493 2667 4162 4565 5083 5626 5788 6117 7096 Size: 1174 1495 403 518 543 162 329 979 Cuts at: 7096 7327 7553 7705 9814 1493 Size: 231 226 152 2109 1697 Fragments arranged by size: 2109 1697 1495 1174 979 543 518 403 329 231 226 162 152 AseI AT'TA_AT Cuts at: 160 1032 2174 8572 160 Size: 872 1142 6398 1606 Fragments arranged by size: 6398 1606 1142 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10018 AvaI C'yCGr_G Cuts at: 1870 1943 5127 5584 6206 7343 1870 Size: 73 3184 457 622 1137 4545 Fragments arranged by size: 4545 3184 1137 622 457 73 AvrII C'CTAG_G Cuts at: 5270 5270 Size: 10018 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5222 5255 5222 Size: 33 9985 BamHI G'GATC_C Cuts at: 6424 7348 7715 6424 Size: 924 367 8727 Fragments arranged by size: 8727 924 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 5275 5802 6026 7339 Size: 325 217 627 597 2891 527 224 1313 Cuts at: 7339 8794 618 Size: 1455 1842 Fragments arranged by size: 2891 1842 1455 1313 627 597 527 325 224 217 BanII G_rGCy'C Cuts at: 1391 5334 5396 5958 6206 6281 7260 7337 1391 Size: 3943 62 562 248 75 979 77 4072 Fragments arranged by size: 4072 3943 979 562 248 77 75 62 BbeI G_GCGC'C Cuts at: 5279 5279 Size: 10018 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3450 5094 5868 6954 928 Size: 971 1551 1644 774 1086 3992 Fragments arranged by size: 3992 1644 1551 1086 971 774 BbvCI CC'TCA_GC Cuts at: 3683 5397 5556 3683 Size: 1714 159 8145 Fragments arranged by size: 8145 1714 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3849 3883 8209 8243 1903 Size: 34 1912 34 4326 34 3678 Fragments arranged by size: 4326 3678 1912 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2806 4614 7910 9120 2806 Size: 1808 3296 1210 3704 Fragments arranged by size: 3704 3296 1808 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10018 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4268 6903 8629 Size: 107 122 71 631 605 2596 2635 1726 Cuts at: 8629 136 Size: 1525 Fragments arranged by size: 2635 2596 1726 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6230 10013 6230 Size: 3783 6235 BlpI GC'TnA_GC Cuts at: 6684 7435 6684 Size: 751 9267 Bme1580I G_kGCm'C Cuts at: 1790 2259 3343 5334 5396 6031 6206 8079 9235 Size: 469 1084 1991 62 635 175 1873 1156 Cuts at: 9235 1790 Size: 2573 Fragments arranged by size: 2573 1991 1873 1156 1084 635 469 175 62 BmgBI CAC'GTC Cuts at: 1318 5319 5486 1318 Size: 4001 167 5850 Fragments arranged by size: 5850 4001 167 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4897 5108 5606 Size: 416 53 83 399 24 3480 211 498 Cuts at: 5606 6184 7749 8707 442 Size: 578 1565 958 1753 Fragments arranged by size: 3480 1753 1565 958 578 498 416 399 211 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4905 1056 Size: 3849 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2885 2885 Size: 10018 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5944 6654 7059 8678 1239 Size: 4705 710 405 1619 2579 Fragments arranged by size: 4705 2579 1619 710 405 Bpu10I CC'TnA_GC Cuts at: 3683 5397 5556 3683 Size: 1714 159 8145 Fragments arranged by size: 8145 1714 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3416 7116 8143 9008 9306 3416 Size: 3700 1027 865 298 4128 Fragments arranged by size: 4128 3700 1027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 8681 882 Size: 617 7182 2219 Fragments arranged by size: 7182 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5851 7809 493 Size: 711 4647 1958 2702 Fragments arranged by size: 4647 2702 1958 711 BsaBI GATnn'nnATC Cuts at: 2592 10012 2592 Size: 7420 2598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5276 5969 7825 8207 Size: 53 83 186 936 3743 693 1856 382 Cuts at: 8207 8842 275 Size: 635 1451 Fragments arranged by size: 3743 1856 1451 936 693 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4935 7252 8451 8849 9123 9270 9681 4935 Size: 2317 1199 398 274 147 411 5272 Fragments arranged by size: 5272 2317 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2574 2604 1178 Size: 30 165 30 1171 30 8592 Fragments arranged by size: 8592 1171 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3713 5572 1186 Size: 2527 1859 5632 Fragments arranged by size: 5632 2527 1859 BseYI C'CCAG_C Cuts at: 5599 5896 6825 7043 9221 9717 5599 Size: 297 929 218 2178 496 5900 Fragments arranged by size: 5900 2178 929 496 297 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2520 2604 2780 4187 6161 6785 1163 Size: 573 784 84 176 1407 1974 624 4396 Fragments arranged by size: 4396 1974 1407 784 624 573 176 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7468 8229 9257 1540 Size: 275 186 5467 761 1028 2301 Fragments arranged by size: 5467 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2301 5312 5958 7075 7260 7286 8079 8164 Size: 42 3011 646 1117 185 26 793 85 Cuts at: 8164 9235 2259 Size: 1071 3042 Fragments arranged by size: 3042 3011 1117 1071 793 646 185 85 42 26 BsmI GAATG_Cn' Cuts at: 6974 7311 7529 7622 6974 Size: 337 218 93 9370 Fragments arranged by size: 9370 337 218 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4587 4587 Size: 10018 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2301 3343 5312 5334 5396 5958 Size: 399 469 42 1042 1969 22 62 562 Cuts at: 5958 6031 6206 6281 7075 7260 7286 7337 8079 Size: 73 175 75 794 185 26 51 742 Cuts at: 8079 8164 9235 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1969 1071 1042 794 742 562 469 399 185 175 85 75 73 62 51 42 26 22 BspEI T'CCGG_A Cuts at: 7252 9681 7252 Size: 2429 7589 BspHI T'CATG_A Cuts at: 4227 7907 9637 4227 Size: 3680 1730 4608 Fragments arranged by size: 4608 3680 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5330 7373 844 Size: 4486 2043 3489 Fragments arranged by size: 4486 3489 2043 BsrBI CCG'CTC Cuts at: 1394 1823 5248 7905 1394 Size: 429 3425 2657 3507 Fragments arranged by size: 3507 3425 2657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4404 4561 6174 8513 8687 65 Size: 1183 3156 157 1613 2339 174 1396 Fragments arranged by size: 3156 2339 1613 1396 1183 174 157 BsrFI r'CCGG_y Cuts at: 1700 1810 8662 8849 1700 Size: 110 6852 187 2869 Fragments arranged by size: 6852 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2409 96 Size: 2313 7705 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10018 BssSI C'ACGA_G Cuts at: 8078 9090 8078 Size: 1012 9006 BstAPI GCAn_nnn'nTGC Cuts at: 794 2767 794 Size: 1973 8045 BstBI TT'CG_AA Cuts at: 5994 8822 5994 Size: 2828 7190 BstEII G'GTnAC_C Cuts at: 1184 4780 1184 Size: 3596 6422 BstXI CCAn_nnnn'nTGG Cuts at: 2430 3388 2430 Size: 958 9060 BstYI r'GATC_y Cuts at: 1972 2764 5167 6230 6424 6770 6913 7246 7348 Size: 792 2403 1063 194 346 143 333 102 Cuts at: 7348 7715 8100 8117 9569 9655 9667 10013 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2403 1977 1452 1063 792 385 367 346 346 333 194 143 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2577 513 Size: 552 1512 7954 Fragments arranged by size: 7954 1512 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9279 Fragments arranged by size: 9279 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3343 3591 4931 5896 6608 7534 8328 8348 Size: 703 248 1340 965 712 926 794 20 Cuts at: 8348 2640 Size: 4310 Fragments arranged by size: 4310 1340 965 926 794 712 703 248 20 ClaI AT'CG_AT Cuts at: 1554 4323 6378 1554 Size: 2769 2055 5194 Fragments arranged by size: 5194 2769 2055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3738 3773 4293 4328 529 Size: 35 3174 35 520 35 6219 Fragments arranged by size: 6219 3174 520 35 35 35 DraI TTT'AAA Cuts at: 7322 7322 Size: 10018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9025 809 Size: 507 7709 1802 Fragments arranged by size: 7709 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2574 4260 Size: 54 1425 50 275 18 286 458 1686 Cuts at: 4260 4492 4928 5734 7465 8354 9983 8 Size: 232 436 806 1731 889 1629 43 Fragments arranged by size: 1731 1686 1629 1425 889 806 458 436 286 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7465 1537 Size: 275 5653 4090 Fragments arranged by size: 5653 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 2669 3245 3643 4035 4636 4959 5317 Size: 726 590 576 398 392 601 323 358 Cuts at: 5317 5991 6356 6515 7279 7948 1353 Size: 674 365 159 764 669 3423 Fragments arranged by size: 3423 764 726 674 669 601 590 576 398 392 365 358 323 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6830 8605 8979 9125 387 Size: 1064 138 5241 1775 374 146 1280 Fragments arranged by size: 5241 1775 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5956 7258 5956 Size: 1302 8716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3951 4343 4563 4992 5789 5880 5944 Size: 393 2319 392 220 429 797 91 64 Cuts at: 5944 5971 6456 6654 7014 7059 8081 8678 9465 Size: 27 485 198 360 45 1022 597 787 Cuts at: 9465 9733 1239 Size: 268 1524 Fragments arranged by size: 2319 1524 1022 797 787 597 485 429 393 392 360 268 220 198 91 64 45 27 EcoO109I rG'GnC_Cy Cuts at: 3679 5109 5264 5331 5393 6202 6203 3679 Size: 1430 155 67 62 809 1 7494 Fragments arranged by size: 7494 1430 809 155 67 62 1 EcoRI G'AATT_C Cuts at: 4162 5626 7327 7705 4162 Size: 1464 1701 378 6475 Fragments arranged by size: 6475 1701 1464 378 EcoRV GAT'ATC Cuts at: 2019 2965 2019 Size: 946 9072 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 10018 FspI TGC'GCA Cuts at: 4508 8524 4508 Size: 4016 6002 HaeII r_GCGC'y Cuts at: 1678 2832 2867 5279 9165 1678 Size: 1154 35 2412 3886 2531 Fragments arranged by size: 3886 2531 2412 1154 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3466 3498 4729 4761 4855 Size: 32 135 32 1894 32 1231 32 94 Cuts at: 4855 4887 5202 5234 5380 5412 6360 6371 6392 Size: 32 315 32 146 32 948 11 21 Cuts at: 6392 6403 6434 6466 6563 6595 6707 6739 8659 Size: 11 31 32 97 32 112 32 1920 Cuts at: 8659 8691 8733 8765 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1920 1894 1231 948 315 146 135 112 97 94 42 32 32 32 32 32 32 32 32 32 32 32 32 31 21 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 2290 2494 4051 7362 7608 669 Size: 988 281 352 204 1557 3311 246 3079 Fragments arranged by size: 3311 3079 1557 988 352 281 246 204 HindIII A'AGCT_T Cuts at: 748 3777 6125 6470 6527 748 Size: 3029 2348 345 57 4239 Fragments arranged by size: 4239 3029 2348 345 57 HpaI GTT'AAC Cuts at: 2290 7608 2290 Size: 5318 4700 KasI G'GCGC_C Cuts at: 5275 5275 Size: 10018 KpnI G_GTAC'C Cuts at: 7343 7343 Size: 10018 MfeI C'AATT_G Cuts at: 7617 7617 Size: 10018 MluI A'CGCG_T Cuts at: 9894 9894 Size: 10018 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3644 5057 9132 9316 1305 Size: 2339 1413 4075 184 2007 Fragments arranged by size: 4075 2339 2007 1413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 2576 4262 4494 4930 Size: 54 1425 343 286 458 1686 232 436 Cuts at: 4930 10 Size: 5098 Fragments arranged by size: 5098 1686 1425 458 436 343 286 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 3050 3386 5305 7976 8335 8494 518 Size: 648 1884 336 1919 2671 359 159 2042 Fragments arranged by size: 2671 2042 1919 1884 648 359 336 159 MspA1I CmG'CkG Cuts at: 1606 5382 6873 7434 8111 9259 9504 1606 Size: 3776 1491 561 677 1148 245 2120 Fragments arranged by size: 3776 2120 1491 1148 677 561 245 NarI GG'CG_CC Cuts at: 5276 5276 Size: 10018 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 7954 Fragments arranged by size: 7954 1512 552 NdeI CA'TA_TG Cuts at: 387 2785 387 Size: 2398 7620 NheI G'CTAG_C Cuts at: 1052 4901 1052 Size: 3849 6169 NotI GC'GGCC_GC Cuts at: 7465 7465 Size: 10018 NspI r_CATG'y Cuts at: 1132 1229 2255 2340 3316 3969 4507 7014 7376 Size: 97 1026 85 976 653 538 2507 362 Cuts at: 7376 8921 1132 Size: 1545 2229 Fragments arranged by size: 2507 2229 1545 1026 976 653 538 362 97 85 PacI TTA_AT'TAA Cuts at: 2178 2178 Size: 10018 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10018 PciI A'CATG_T Cuts at: 3965 8917 3965 Size: 4952 5066 PflMI CCAn_nnn'nTGG Cuts at: 1108 3575 1108 Size: 2467 7551 PmeI GTTT'AAAC Cuts at: 7322 7322 Size: 10018 PmlI CAC'GTG Cuts at: 5851 5851 Size: 10018 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4778 4810 7135 7167 8087 8119 9600 9632 4778 Size: 32 2325 32 920 32 1481 32 5164 Fragments arranged by size: 5164 2325 1481 920 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3679 5109 5264 3679 Size: 1430 155 8433 Fragments arranged by size: 8433 1430 155 PshAI GACnn'nnGTC Cuts at: 1613 3286 4806 1613 Size: 1673 1520 6825 Fragments arranged by size: 6825 1673 1520 PsiI TTA'TAA Cuts at: 3023 3228 7588 3023 Size: 205 4360 5453 Fragments arranged by size: 5453 4360 205 PspOMI G'GGCC_C Cuts at: 5330 5392 6202 5330 Size: 62 810 9146 Fragments arranged by size: 9146 810 62 PspXI vC'TCGA_Gb Cuts at: 5584 5584 Size: 10018 PstI C_TGCA'G Cuts at: 830 7370 830 Size: 6540 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10018 SacI G_AGCT'C Cuts at: 5958 7260 5958 Size: 1302 8716 SalI G'TCGA_C Cuts at: 1936 7360 1936 Size: 5424 4594 SapI GCTCTTCn'nnn_ Cuts at: 2079 5317 5991 2079 Size: 3238 674 6106 Fragments arranged by size: 6106 3238 674 SbfI CC_TGCA'GG Cuts at: 7370 7370 Size: 10018 ScaI AGT'ACT Cuts at: 5823 8266 9978 5823 Size: 2443 1712 5863 Fragments arranged by size: 5863 2443 1712 SfcI C'TryA_G Cuts at: 826 1045 3093 3238 4098 4614 6163 7366 8501 Size: 219 2048 145 860 516 1549 1203 1135 Cuts at: 8501 9182 9373 826 Size: 681 191 1471 Fragments arranged by size: 2048 1549 1471 1203 1135 860 681 516 219 191 145 SfoI GGC'GCC Cuts at: 5277 5277 Size: 10018 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10018 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10018 SmaI CCC'GGG Cuts at: 1872 6208 7345 1872 Size: 4336 1137 4545 Fragments arranged by size: 4545 4336 1137 SmlI C'TyrA_G Cuts at: 820 1017 1943 3431 5127 5584 6530 7131 8122 Size: 197 926 1488 1696 457 946 601 991 Cuts at: 8122 9023 9285 820 Size: 901 262 1553 Fragments arranged by size: 1696 1553 1488 991 946 926 901 601 457 262 197 SnaBI TAC'GTA Cuts at: 493 7809 493 Size: 7316 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10018 SphI G_CATG'C Cuts at: 1132 7014 7376 1132 Size: 5882 362 3774 Fragments arranged by size: 5882 3774 362 SspI AAT'ATT Cuts at: 5 52 3232 3270 4837 5029 7942 5 Size: 47 3180 38 1567 192 2913 2081 Fragments arranged by size: 3180 2913 2081 1567 192 47 38 StuI AGG'CCT Cuts at: 1517 7147 1517 Size: 5630 4388 StyI C'CwwG_G Cuts at: 513 1065 2577 4660 5270 5447 7457 513 Size: 552 1512 2083 610 177 2010 3074 Fragments arranged by size: 3074 2083 2010 1512 610 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3394 3582 5587 8045 8062 8215 8400 Size: 1255 1814 188 2005 2458 17 153 185 Cuts at: 8400 8845 325 Size: 445 1498 Fragments arranged by size: 2458 2005 1814 1498 1255 445 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2409 5821 8264 Size: 274 80 33 51 1443 432 3412 2443 Cuts at: 8264 9976 96 Size: 1712 138 Fragments arranged by size: 3412 2443 1712 1443 432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2574 2606 2772 2804 3570 3602 6304 6336 9978 Size: 32 166 32 766 32 2702 32 3642 Cuts at: 9978 10010 2574 Size: 32 2582 Fragments arranged by size: 3642 2702 2582 766 166 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4352 4373 1316 Size: 3036 21 6961 Fragments arranged by size: 6961 3036 21 XbaI T'CTAG_A Cuts at: 7354 7354 Size: 10018 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3572 3572 Size: 10018 XhoI C'TCGA_G Cuts at: 1943 5127 5584 1943 Size: 3184 457 6377 Fragments arranged by size: 6377 3184 457 XmaI C'CCGG_G Cuts at: 1870 6206 7343 1870 Size: 4336 1137 4545 Fragments arranged by size: 4545 4336 1137 XmnI GAAnn'nnTTC Cuts at: 2055 2856 3422 3458 4645 4977 7311 8147 2055 Size: 801 566 36 1187 332 2334 836 3926 Fragments arranged by size: 3926 2334 1187 836 801 566 332 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7826 276 Size: 53 83 186 936 6292 2468 Fragments arranged by size: 6292 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PfoI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI