################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BspDI BspEI BspLU11I BsrDI BsrI BssHII BssSI BstAPI BstEII BstF5I BstPI BstXI Bsu36I CelII ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MslI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 118 GTATAC GT^[AC][GT]AC AflIII 122 ACGTGT A^C[GA][CT]GT AluI 109 AGCT AG^CT AlwI 282 GATCC GGATC(4/5) Asp700I 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 310 CCCGGG C^[CT]CG[GA]G AvaII 144 GGTCC G^G[AT]CC BalI 320 TGGCCA TGG^CCA BanI 343 GGCACC G^G[CT][GA]CC BanII 39 GGGCTC G[GA]GC[CT]^C BpmI 273 CTCCAG CTGGAG(16/14) BsaAI 121 TACGTG [CT]AC^GT[GA] BseRI 165 CTCCTC GAGGAG(10/8) BsiEI 290 CGGCCG CG[GA][CT]^CG BsoBI 310 CCCGGG C^[CT]CG[GA]G Bsp1407I 125 TGTACA T^GTACA BspHI 185 TCATGA T^CATGA BspMI 141 GCAGGT ACCTGC(4/8) BsrBI 20 CCGCTC CCGCTC(-3/-3) BsrFI 157 ACCGGC [GA]^CCGG[CT] BsrGI 125 TGTACA T^GTACA Bst1107I 118 GTATAC GTA^TAC BstBI 211 TTCGAA TT^CGAA BstYI 281 AGATCC [GA]^GATC[CT] BstZ17I 118 GTATAC GTA^TAC Cfr10I 157 ACCGGC [GA]^CCGG[CT] Cfr13I 144 GGTCC G^G[ACGT]CC DpnII 282 GATC ^GATC DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG EagI 290 CGGCCG C^GGCCG EclXI 290 CGGCCG C^GGCCG Eco52I 290 CGGCCG C^GGCCG HincII 199 GTCAAC GT[CT]^[GA]AC HindII 199 GTCAAC GT[CT]^[GA]AC HinfI 182 GAATC G^A[ACGT]TC MaeII 122 ACGT A^CGT MboI 282 GATC ^GATC MflI 281 AGATCC [GA]^GATC[CT] MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA NcoI 176 CCATGG C^CATGG NdeII 282 GATC ^GATC NlaIV 343 GGCACC GG[ACGT]^[ACGT]CC NspV 211 TTCGAA TT^CGAA RcaI 185 TCATGA T^CATGA Sau3AI 282 GATC ^GATC Sau96I 144 GGTCC G^G[ACGT]CC SfuI 211 TTCGAA TT^CGAA SmaI 310 CCCGGG CCC^GGG SspBI 125 TGTACA T^GTACA TfiI 182 GAATC G^A[AT]TC XhoII 281 AGATCC [GA]^GATC[CT] XmaCI 310 CCCGGG C^CCGGG XmaI 310 CCCGGG C^CCGGG XmnI 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 27 CGCG CG^CG 222 CGCG AfaI 126 GTAC GT^AC 363 GTAC Aor51HI 257 AGCGCT AGC^GCT 370 AGCGCT BcnI 310 CCCGG CC^[GC]GG 311 CCGGG BmyI 39 GGGCTC G[AGT]GC[ACT]^C 342 GGGCAC BsiYI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGACACCGG BslI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 151 CCCGACACCGG Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C 342 GGGCAC BstNI 240 CCTGG CC^[AT]GG 275 CCAGG BstUI 27 CGCG CG^CG 222 CGCG EaeI 290 CGGCCG [CT]^GGCC[GA] 320 TGGCCA Eco47III 257 AGCGCT AGC^GCT 370 AGCGCT EcoRII 240 CCTGG ^CC[AT]GG 275 CCAGG EcoT14I 176 CCATGG C^C[AT][AT]GG 347 CCAAGG HaeII 257 AGCGCT [GA]GCGC^[CT] 370 AGCGCT HapII 158 CCGG C^CGG 311 CCGG HpaII 158 CCGG C^CGG 311 CCGG MaeIII 55 GTGAC ^GT[ACGT]AC 355 GTCAC MspI 158 CCGG C^CGG 311 CCGG MvaI 240 CCTGG CC^[AT]GG 275 CCAGG MvnI 27 CGCG CG^CG 222 CGCG NciI 310 CCCGG CC^[GC]GG 311 CCGGG NlaIII 177 CATG CATG^ 186 CATG RsaI 126 GTAC GT^AC 363 GTAC SfaNI 161 GCATC GCATC(5/9) 218 GCATC StyI 176 CCATGG C^C[AT][AT]GG 347 CCAAGG Tsp45I 55 GTGAC ^GT[GC]AC 355 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI BbvI BsaJI BssKI Cac8I CfoI DdeI FauI Fnu4HI HaeIII HhaI HinP1I HphI ItaI MboII MnlI MwoI ScrFI TaqI TseI TthHB8I