(Circular) (Six-base) MAPSORT of: pFN21AB6487.seq Check: 6389 from: 1 to: 5692 pFN21AB6487 5692 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2550 3755 5139 5407 1632 Size: 918 1205 1384 268 1917 Fragments arranged by size: 1917 1384 1205 918 268 AflII C'TTAA_G Cuts at: 820 1017 2582 820 Size: 197 1565 3930 Fragments arranged by size: 3930 1565 197 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2389 2491 3435 5007 2389 Size: 102 944 1572 3074 Fragments arranged by size: 3074 1572 944 102 ApaI G_GGCC'C Cuts at: 2027 2828 2027 Size: 801 4891 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 2762 3001 3227 3379 5488 1493 Size: 1269 239 226 152 2109 1697 Fragments arranged by size: 2109 1697 1269 239 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2068 2075 2472 2820 3017 1870 Size: 73 77 48 7 397 348 197 4545 Fragments arranged by size: 4545 397 348 197 77 73 48 7 BamHI G'GATC_C Cuts at: 2925 3022 3389 2925 Size: 97 367 5228 Fragments arranged by size: 5228 367 97 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2937 3013 4468 618 Size: 325 217 627 1150 76 1455 1842 Fragments arranged by size: 1842 1455 1150 627 325 217 76 BanII G_rGCy'C Cuts at: 1391 2027 2828 3011 1391 Size: 636 801 183 4072 Fragments arranged by size: 4072 801 636 183 BbeI G_GCGC'C Cuts at: 2941 2941 Size: 5692 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2974 928 Size: 971 1075 3646 Fragments arranged by size: 3646 1075 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2039 4303 Size: 107 122 71 631 605 337 30 2264 Cuts at: 4303 136 Size: 1525 Fragments arranged by size: 2264 1525 631 605 337 122 107 71 30 BglII A'GATC_T Cuts at: 2664 5687 2664 Size: 3023 2669 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2027 2828 3753 4909 1790 Size: 237 801 925 1156 2573 Fragments arranged by size: 2573 1156 925 801 237 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 3423 4381 Size: 416 53 83 399 24 937 1069 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1069 958 937 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 2886 4352 1239 Size: 1625 22 1466 2579 Fragments arranged by size: 2579 1625 1466 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2087 2627 3817 4682 4980 2087 Size: 540 1190 865 298 2799 Fragments arranged by size: 2799 1190 865 540 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 2105 2783 4355 882 Size: 617 579 27 678 1572 2219 Fragments arranged by size: 2219 1572 678 617 579 27 BsaAI yAC'GTr Cuts at: 493 1204 2131 3483 493 Size: 711 927 1352 2702 Fragments arranged by size: 2702 1352 927 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2938 3499 3881 4516 Size: 53 83 186 936 1405 561 382 635 Cuts at: 4516 275 Size: 1451 Fragments arranged by size: 1451 1405 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 4125 4523 4797 4944 5355 2265 Size: 1860 398 274 147 411 2602 Fragments arranged by size: 2602 1860 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2238 2260 2268 2290 2420 Size: 30 165 30 835 22 8 22 130 Cuts at: 2420 2450 2534 2564 1178 Size: 30 84 30 4306 Fragments arranged by size: 4306 835 165 130 84 30 30 30 30 22 22 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2801 1186 Size: 1306 309 4077 Fragments arranged by size: 4077 1306 309 BseYI C'CCAG_C Cuts at: 2356 2833 4895 5391 2356 Size: 477 2062 496 2657 Fragments arranged by size: 2657 2062 496 477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2510 2701 1163 Size: 573 666 108 191 4154 Fragments arranged by size: 4154 666 573 191 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2221 2275 3142 3903 4931 1540 Size: 275 186 220 54 867 761 1028 2301 Fragments arranged by size: 2301 1028 867 761 275 220 186 54 BsiHKAI G_wGCw'C Cuts at: 2913 3753 3838 4909 2913 Size: 840 85 1071 3696 Fragments arranged by size: 3696 1071 840 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2828 2913 3011 3753 3838 4909 Size: 399 237 801 85 98 742 85 1071 Cuts at: 4909 1391 Size: 2174 Fragments arranged by size: 2174 1071 801 742 399 237 98 85 85 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 3047 844 Size: 1466 737 3489 Fragments arranged by size: 3489 1466 737 BsrBI CCG'CTC Cuts at: 1394 1823 2271 3579 1394 Size: 429 448 1308 3507 Fragments arranged by size: 3507 1308 448 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2512 2512 Size: 5692 BstYI r'GATC_y Cuts at: 1972 2664 2778 2925 3022 3389 3774 3791 5243 Size: 692 114 147 97 367 385 17 1452 Cuts at: 5243 5329 5341 5687 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 692 385 367 346 147 114 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2457 2511 2623 2884 513 Size: 552 972 420 54 112 261 3321 Fragments arranged by size: 3321 972 552 420 261 112 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2321 3208 4002 4022 2321 Size: 887 794 20 3991 Fragments arranged by size: 3991 887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2272 2493 Size: 54 1425 50 275 18 388 54 221 Cuts at: 2493 2881 2968 3139 4028 5657 8 Size: 388 87 171 889 1629 43 Fragments arranged by size: 1629 1425 889 388 388 275 221 171 87 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2218 2272 3139 1537 Size: 275 406 54 867 4090 Fragments arranged by size: 4090 867 406 275 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 2892 3622 1353 Size: 1289 250 730 3423 Fragments arranged by size: 3423 1289 730 250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4279 4653 4799 387 Size: 1064 138 438 2252 374 146 1280 Fragments arranged by size: 2252 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 2864 2886 3755 4352 5139 5407 Size: 393 918 314 22 869 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 918 869 787 597 393 314 268 22 EcoNI CCTnn'n_nnAGG Cuts at: 2381 2393 2843 2381 Size: 12 450 5230 Fragments arranged by size: 5230 450 12 EcoO109I rG'GnC_Cy Cuts at: 2386 2824 2386 Size: 438 5254 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 2174 4198 2174 Size: 2024 3668 HaeII r_GCGC'y Cuts at: 1678 2414 2941 4839 1678 Size: 736 527 1898 2531 Fragments arranged by size: 2531 1898 736 527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2260 2292 2420 2452 4333 Size: 32 135 32 688 32 128 32 1881 Cuts at: 4333 4365 4407 4439 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1881 688 135 128 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KasI G'GCGC_C Cuts at: 2937 2937 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 2970 10 Size: 54 1425 343 663 475 2732 Fragments arranged by size: 2732 1425 663 475 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 3650 4009 4168 518 Size: 648 1290 1194 359 159 2042 Fragments arranged by size: 2042 1290 1194 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2110 2217 2407 2443 2864 2886 3108 Size: 433 71 107 190 36 421 22 222 Cuts at: 3108 3785 4933 5178 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 433 421 245 222 190 107 71 36 22 NarI GG'CG_CC Cuts at: 2938 2938 Size: 5692 NcoI C'CATG_G Cuts at: 513 1065 2457 513 Size: 552 1392 3748 Fragments arranged by size: 3748 1392 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 2218 3139 2218 Size: 921 4771 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PfoI T'CCnGG_A Cuts at: 2841 2841 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2958 1613 Size: 1345 4347 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2023 2824 2023 Size: 801 4891 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2111 2143 2111 Size: 32 5660 PstI C_TGCA'G Cuts at: 830 2418 3044 830 Size: 1588 626 3478 Fragments arranged by size: 3478 1588 626 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2407 2864 2407 Size: 457 5235 SacII CC_GC'GG Cuts at: 2040 2887 2040 Size: 847 4845 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 2549 3940 5652 2549 Size: 1391 1712 2589 Fragments arranged by size: 2589 1712 1391 SexAI A'CCwGG_T Cuts at: 2376 2376 Size: 5692 SfcI C'TryA_G Cuts at: 826 1045 2414 3040 4175 4856 5047 826 Size: 219 1369 626 1135 681 191 1471 Fragments arranged by size: 1471 1369 1135 681 626 219 191 SfoI GGC'GCC Cuts at: 2939 2939 Size: 5692 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 2474 2822 3019 1872 Size: 602 348 197 4545 Fragments arranged by size: 4545 602 348 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2102 2582 2642 3796 4697 Size: 197 926 132 27 480 60 1154 901 Cuts at: 4697 4959 820 Size: 262 1553 Fragments arranged by size: 1553 1154 926 901 480 262 197 132 60 27 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 2018 1517 Size: 501 5191 StyI C'CwwG_G Cuts at: 513 1065 2457 2754 3131 513 Size: 552 1392 297 377 3074 Fragments arranged by size: 3074 1392 552 377 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2242 2252 2963 3719 3736 3889 4074 Size: 1255 662 10 711 756 17 153 185 Cuts at: 4074 4519 325 Size: 445 1498 Fragments arranged by size: 1498 1255 756 711 662 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 3938 5650 Size: 274 80 33 51 1443 570 1391 1712 Cuts at: 5650 96 Size: 138 Fragments arranged by size: 1712 1443 1391 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 5652 5684 2238 Size: 32 3382 32 2246 Fragments arranged by size: 3382 2246 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2434 1316 Size: 1118 4574 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 2075 1943 Size: 132 5560 XmaI C'CCGG_G Cuts at: 1870 2472 2820 3017 1870 Size: 602 348 197 4545 Fragments arranged by size: 4545 602 348 197 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI