(Circular) (Six-base) MAPSORT of: pFN21AB7330.seq Check: 6927 from: 1 to: 7321 pFN21AB7330 7321 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3705 5131 278 Size: 53 83 186 936 2169 1426 2468 Fragments arranged by size: 2468 2169 1426 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2034 4044 4664 1937 Size: 97 2010 620 4594 Fragments arranged by size: 4594 2010 620 97 Acc65I G'GTAC_C Cuts at: 2463 4642 2463 Size: 2179 5142 AclI AA'CG_TT Cuts at: 1563 5448 5821 1563 Size: 3885 373 3063 Fragments arranged by size: 3885 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2978 4393 4402 5384 6768 7036 1632 Size: 452 894 1415 9 982 1384 268 1917 Fragments arranged by size: 1917 1415 1384 982 894 452 268 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7124 AflIII A'CryG_T Cuts at: 1203 2298 4359 6220 7197 1203 Size: 1095 2061 1861 977 1327 Fragments arranged by size: 2061 1861 1327 1095 977 AgeI A'CCGG_T Cuts at: 6152 6152 Size: 7321 AhdI GACnn_n'nnGTC Cuts at: 3739 6050 3739 Size: 2311 5010 AleI CACnn'nnGTG Cuts at: 1166 2809 1166 Size: 1643 5678 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2470 3086 1184 Size: 1286 616 5419 Fragments arranged by size: 5419 1286 616 AlwNI CAG_nnn'CTG Cuts at: 2620 5064 6636 2620 Size: 2444 1572 3305 Fragments arranged by size: 3305 2444 1572 ApaI G_GGCC'C Cuts at: 2380 3276 3794 3827 4557 4614 2380 Size: 896 518 33 730 57 5087 Fragments arranged by size: 5087 896 730 518 57 33 ApaLI G'TGCA_C Cuts at: 5378 6534 5378 Size: 1156 6165 ApoI r'AATT_y Cuts at: 1493 2239 4630 4856 5008 7117 1493 Size: 746 2391 226 152 2109 1697 Fragments arranged by size: 2391 2109 1697 746 226 152 AscI GG'CGCG_CC Cuts at: 2094 2094 Size: 7321 AseI AT'TA_AT Cuts at: 160 1032 5875 160 Size: 872 4843 1606 Fragments arranged by size: 4843 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 AvaI C'yCGr_G Cuts at: 1870 1943 2044 2109 3345 3422 3604 3845 3872 Size: 73 101 65 1236 77 182 241 27 Cuts at: 3872 4318 4334 4646 1870 Size: 446 16 312 4545 Fragments arranged by size: 4545 1236 446 312 241 182 101 77 73 65 27 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4525 4558 4525 Size: 33 7288 BamHI G'GATC_C Cuts at: 4651 5018 4651 Size: 367 6954 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2463 2651 3442 3503 Size: 325 217 627 405 271 188 791 61 Cuts at: 3503 4003 4111 4190 4477 4642 6097 618 Size: 500 108 79 287 165 1455 1842 Fragments arranged by size: 1842 1455 791 627 500 405 325 287 271 217 188 165 108 79 61 BanII G_rGCy'C Cuts at: 1391 2369 2380 2672 3276 3794 3827 4557 4614 Size: 978 11 292 604 518 33 730 57 Cuts at: 4614 4640 1391 Size: 26 4072 Fragments arranged by size: 4072 978 730 604 518 292 57 33 26 11 BbeI G_GCGC'C Cuts at: 2196 4007 4115 4481 2196 Size: 1811 108 366 5036 Fragments arranged by size: 5036 1811 366 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3325 4032 928 Size: 971 1426 707 4217 Fragments arranged by size: 4217 1426 971 707 BbvCI CC'TCA_GC Cuts at: 2165 3185 2165 Size: 1020 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5512 5546 1903 Size: 34 3575 34 3678 Fragments arranged by size: 3678 3575 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5213 6423 5213 Size: 1210 6111 BclI T'GATC_A Cuts at: 1548 2820 1548 Size: 1272 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3348 5932 136 Size: 107 122 71 631 605 1676 2584 1525 Fragments arranged by size: 2584 1676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7316 7316 Size: 7321 BlpI GC'TnA_GC Cuts at: 4738 4738 Size: 7321 Bme1580I G_kGCm'C Cuts at: 1790 2380 3114 3276 3447 3481 3794 3827 4557 Size: 590 734 162 171 34 313 33 730 Cuts at: 4557 4614 5382 6538 1790 Size: 57 768 1156 2573 Fragments arranged by size: 2573 1156 768 734 730 590 313 171 162 57 34 33 BmgBI CAC'GTC Cuts at: 1318 3694 4362 4597 1318 Size: 2376 668 235 4042 Fragments arranged by size: 4042 2376 668 235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4423 5052 6010 Size: 416 53 83 399 24 3006 629 958 Cuts at: 6010 442 Size: 1753 Fragments arranged by size: 3006 1753 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7321 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 3733 2383 Size: 1350 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2494 3055 3133 3197 3322 3475 4063 Size: 1040 215 561 78 64 125 153 588 Cuts at: 4063 4418 5981 1239 Size: 355 1563 2579 Fragments arranged by size: 2579 1563 1040 588 561 355 215 153 125 78 64 Bpu10I CC'TnA_GC Cuts at: 2165 3185 2165 Size: 1020 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 3179 5446 6311 6609 2471 Size: 708 2267 865 298 3183 Fragments arranged by size: 3183 2267 865 708 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3959 5984 882 Size: 617 2460 2025 2219 Fragments arranged by size: 2460 2219 2025 617 BsaAI yAC'GTr Cuts at: 493 1204 2299 3081 5112 493 Size: 711 1095 782 2031 2702 Fragments arranged by size: 2702 2031 1095 782 711 BsaBI GATnn'nnATC Cuts at: 2519 3950 7315 2519 Size: 1431 3365 2525 Fragments arranged by size: 3365 2525 1431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 3702 4004 4112 Size: 53 83 186 936 660 1509 302 108 Cuts at: 4112 4478 5128 5510 6145 275 Size: 366 650 382 635 1451 Fragments arranged by size: 1509 1451 936 660 650 635 382 366 302 186 108 83 53 BsaWI w'CCGG_w Cuts at: 2595 5754 6152 6426 6573 6984 2595 Size: 3159 398 274 147 411 2932 Fragments arranged by size: 3159 2932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3059 3089 3131 3161 4178 Size: 30 165 30 1656 30 42 30 1017 Cuts at: 4178 4199 4208 4229 4502 4514 4532 4544 1178 Size: 21 9 21 273 12 18 12 3955 Fragments arranged by size: 3955 1656 1017 273 165 42 30 30 30 30 21 21 18 12 12 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4406 1186 Size: 3220 4101 BseYI C'CCAG_C Cuts at: 2274 2665 3404 3434 3793 3854 4602 6524 7020 Size: 391 739 30 359 61 748 1922 496 Cuts at: 7020 2274 Size: 2575 Fragments arranged by size: 2575 1922 748 739 496 391 359 61 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 3056 3286 1163 Size: 573 294 1026 230 5198 Fragments arranged by size: 5198 1026 573 294 230 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2267 3831 4122 4256 4473 4771 Size: 275 186 266 1564 291 134 217 298 Cuts at: 4771 5532 6560 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 298 291 275 266 217 186 134 BsiHKAI G_wGCw'C Cuts at: 2369 2532 5382 5467 6538 2369 Size: 163 2850 85 1071 3152 Fragments arranged by size: 3152 2850 1071 163 85 BsmI GAATG_Cn' Cuts at: 4832 4925 4832 Size: 93 7228 BsmBI CGTCTCn'nnnn_ Cuts at: 3249 3249 Size: 7321 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2380 2532 2672 3114 3276 3447 Size: 399 579 11 152 140 442 162 171 Cuts at: 3447 3481 3794 3827 4557 4614 4640 5382 5467 Size: 34 313 33 730 57 26 742 85 Cuts at: 5467 6538 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 730 579 442 399 313 171 162 152 140 85 57 34 33 26 11 BspEI T'CCGG_A Cuts at: 2595 6984 2595 Size: 4389 2932 BspHI T'CATG_A Cuts at: 5210 6940 5210 Size: 1730 5591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3078 3880 4353 4676 844 Size: 2234 802 473 323 3489 Fragments arranged by size: 3489 2234 802 473 323 BsrBI CCG'CTC Cuts at: 1394 1823 2049 2263 4252 5208 1394 Size: 429 226 214 1989 956 3507 Fragments arranged by size: 3507 1989 956 429 226 214 BsrDI GCAATG_nn' Cuts at: 65 1248 2541 4503 5816 5990 65 Size: 1183 1293 1962 1313 174 1396 Fragments arranged by size: 1962 1396 1313 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2132 2460 2558 2640 3722 4000 4574 Size: 110 322 328 98 82 1082 278 574 Cuts at: 4574 5965 6152 1700 Size: 1391 187 2869 Fragments arranged by size: 2869 1391 1082 574 328 322 278 187 110 98 82 BsrGI T'GTAC_A Cuts at: 96 3117 96 Size: 3021 4300 BssHII G'CGCG_C Cuts at: 1925 2094 1925 Size: 169 7152 BssSI C'ACGA_G Cuts at: 2324 4247 4388 5381 6393 2324 Size: 1923 141 993 1012 3252 Fragments arranged by size: 3252 1923 1012 993 141 BstAPI GCAn_nnn'nTGC Cuts at: 794 2620 3264 794 Size: 1826 644 4851 Fragments arranged by size: 4851 1826 644 BstBI TT'CG_AA Cuts at: 6125 6125 Size: 7321 BstEII G'GTnAC_C Cuts at: 1184 3103 3399 1184 Size: 1919 296 5106 Fragments arranged by size: 5106 1919 296 BstXI CCAn_nnnn'nTGG Cuts at: 3862 3862 Size: 7321 BstYI r'GATC_y Cuts at: 1972 2436 4651 5018 5403 5420 6872 6958 6970 Size: 464 2215 367 385 17 1452 86 12 Cuts at: 6970 7316 1972 Size: 346 1977 Fragments arranged by size: 2215 1977 1452 464 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2035 2035 Size: 7321 BtgI C'CryG_G Cuts at: 513 1065 2004 2467 3900 4089 4186 4200 4210 Size: 552 939 463 1433 189 97 14 10 Cuts at: 4210 4380 4473 513 Size: 170 93 3361 Fragments arranged by size: 3361 1433 939 552 463 189 170 97 93 14 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2518 487 Size: 683 56 1292 5290 Fragments arranged by size: 5290 1292 683 56 BtsI GCAGTG_nn' Cuts at: 2703 4837 5631 5651 2703 Size: 2134 794 20 4373 Fragments arranged by size: 4373 2134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7321 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7286 DraI TTT'AAA Cuts at: 4625 4625 Size: 7321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6328 809 Size: 507 5012 1802 Fragments arranged by size: 5012 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2264 2553 3724 Size: 54 1425 50 275 18 434 289 1171 Cuts at: 3724 3828 4119 4470 4569 4768 5657 7286 8 Size: 104 291 351 99 199 889 1629 43 Fragments arranged by size: 1629 1425 1171 889 434 351 291 289 275 199 104 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2264 3828 4119 4470 4768 1537 Size: 275 452 1564 291 351 298 4090 Fragments arranged by size: 4090 1564 452 351 298 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2357 2990 5251 1353 Size: 1004 633 2261 3423 Fragments arranged by size: 3423 2261 1004 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3016 3851 4131 4252 5908 6282 Size: 1064 138 1427 835 280 121 1656 374 Cuts at: 6282 6428 387 Size: 146 1280 Fragments arranged by size: 1656 1427 1280 1064 835 374 280 146 138 121 EcoICRI GAG'CTC Cuts at: 2367 2367 Size: 7321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2279 2494 2978 3055 3133 3197 Size: 393 452 195 215 484 77 78 64 Cuts at: 3197 3322 3475 4063 4393 4402 4418 5384 5981 Size: 125 153 588 330 9 16 966 597 Cuts at: 5981 6768 7036 1239 Size: 787 268 1524 Fragments arranged by size: 1524 966 787 597 588 484 452 393 330 268 215 195 153 125 78 77 64 16 9 EcoNI CCTnn'n_nnAGG Cuts at: 4304 4304 Size: 7321 EcoO109I rG'GnC_Cy Cuts at: 2143 2376 2377 2815 3144 3226 3272 3273 3372 Size: 233 1 438 329 82 46 1 99 Cuts at: 3372 3595 3790 3823 3850 4329 4497 4554 4583 Size: 223 195 33 27 479 168 57 29 Cuts at: 4583 2143 Size: 4881 Fragments arranged by size: 4881 479 438 329 233 223 195 168 99 82 57 46 33 29 27 1 1 EcoRI G'AATT_C Cuts at: 4630 5008 4630 Size: 378 6943 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2089 3910 3934 2089 Size: 1821 24 5476 Fragments arranged by size: 5476 1821 24 FspI TGC'GCA Cuts at: 4226 5827 4226 Size: 1601 5720 FspAI rTGC'GCAy Cuts at: 4226 4226 Size: 7321 HaeII r_GCGC'y Cuts at: 1678 2123 2196 3411 4007 4115 4481 6468 1678 Size: 445 73 1215 596 108 366 1987 2531 Fragments arranged by size: 2531 1987 1215 596 445 366 108 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3059 3091 3725 3757 4199 Size: 32 135 32 1487 32 634 32 442 Cuts at: 4199 4231 4502 4514 4534 4546 5962 5994 6036 Size: 32 271 12 20 12 1416 32 42 Cuts at: 6036 6068 1373 Size: 32 2626 Fragments arranged by size: 2626 1487 1416 634 442 271 135 42 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4045 4665 4911 669 Size: 988 281 2107 620 246 3079 Fragments arranged by size: 3079 2107 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7321 HpaI GTT'AAC Cuts at: 4911 4911 Size: 7321 KasI G'GCGC_C Cuts at: 2192 4003 4111 4477 2192 Size: 1811 108 366 5036 Fragments arranged by size: 5036 1811 366 108 KpnI G_GTAC'C Cuts at: 2467 4646 2467 Size: 2179 5142 MfeI C'AATT_G Cuts at: 4920 4920 Size: 7321 MluI A'CGCG_T Cuts at: 7197 7197 Size: 7321 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4557 6435 6619 1305 Size: 3252 1878 184 2007 Fragments arranged by size: 3252 2007 1878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5499 Fragments arranged by size: 5499 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 2546 2809 5279 5638 5797 518 Size: 648 1359 21 263 2470 359 159 2042 Fragments arranged by size: 2470 2042 1359 648 359 263 159 21 MspA1I CmG'CkG Cuts at: 1606 3166 3175 3943 4413 4461 4737 5414 6562 Size: 1560 9 768 470 48 276 677 1148 Cuts at: 6562 6807 1606 Size: 245 2120 Fragments arranged by size: 2120 1560 1148 768 677 470 276 245 48 9 NaeI GCC'GGC Cuts at: 2134 2560 3724 4002 2134 Size: 426 1164 278 5453 Fragments arranged by size: 5453 1164 426 278 NarI GG'CG_CC Cuts at: 2193 4004 4112 4478 2193 Size: 1811 108 366 5036 Fragments arranged by size: 5036 1811 366 108 NcoI C'CATG_G Cuts at: 513 1065 2004 3900 513 Size: 552 939 1896 3934 Fragments arranged by size: 3934 1896 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7321 NgoMIV G'CCGG_C Cuts at: 2132 2558 3722 4000 2132 Size: 426 1164 278 5453 Fragments arranged by size: 5453 1164 426 278 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7321 NotI GC'GGCC_GC Cuts at: 2264 4768 2264 Size: 2504 4817 NspI r_CATG'y Cuts at: 1132 1229 3739 3940 4043 4679 6224 1132 Size: 97 2510 201 103 636 1545 2229 Fragments arranged by size: 2510 2229 1545 636 201 103 97 PasI CC'CwG_GG Cuts at: 1348 2566 1348 Size: 1218 6103 PciI A'CATG_T Cuts at: 6220 6220 Size: 7321 PflMI CCAn_nnn'nTGG Cuts at: 1108 3453 3781 4155 1108 Size: 2345 328 374 4274 Fragments arranged by size: 4274 2345 374 328 PfoI T'CCnGG_A Cuts at: 2388 2388 Size: 7321 PmeI GTTT'AAAC Cuts at: 4625 4625 Size: 7321 PmlI CAC'GTG Cuts at: 3081 3081 Size: 7321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5390 5422 6903 6935 5390 Size: 32 1481 32 5776 Fragments arranged by size: 5776 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3226 3372 3595 3850 4583 3226 Size: 146 223 255 733 5964 Fragments arranged by size: 5964 733 255 223 146 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7321 PsiI TTA'TAA Cuts at: 4891 4891 Size: 7321 PspOMI G'GGCC_C Cuts at: 2376 3272 3790 3823 4553 4610 2376 Size: 896 518 33 730 57 5087 Fragments arranged by size: 5087 896 730 518 57 33 PspXI vC'TCGA_Gb Cuts at: 3422 3422 Size: 7321 PstI C_TGCA'G Cuts at: 830 2238 2616 2932 3762 4328 4673 830 Size: 1408 378 316 830 566 345 3478 Fragments arranged by size: 3478 1408 830 566 378 345 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7321 PvuII CAG'CTG Cuts at: 3175 4461 3175 Size: 1286 6035 SacI G_AGCT'C Cuts at: 2369 2369 Size: 7321 SalI G'TCGA_C Cuts at: 1936 4043 4663 1936 Size: 2107 620 4594 Fragments arranged by size: 4594 2107 620 SanDI GG'GwC_CC Cuts at: 3372 4583 3372 Size: 1211 6110 SapI GCTCTTCn'nnn_ Cuts at: 2357 2357 Size: 7321 SbfI CC_TGCA'GG Cuts at: 2932 4673 2932 Size: 1741 5580 ScaI AGT'ACT Cuts at: 2426 3089 3716 5569 7281 2426 Size: 663 627 1853 1712 2466 Fragments arranged by size: 2466 1853 1712 663 627 SfcI C'TryA_G Cuts at: 826 1045 2234 2612 2928 3758 4324 4669 5804 Size: 219 1189 378 316 830 566 345 1135 Cuts at: 5804 6485 6676 826 Size: 681 191 1471 Fragments arranged by size: 1471 1189 1135 830 681 566 378 345 316 219 191 SfoI GGC'GCC Cuts at: 2194 4005 4113 4479 2194 Size: 1811 108 366 5036 Fragments arranged by size: 5036 1811 366 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 SgrAI Cr'CCGG_yG Cuts at: 1700 4574 1700 Size: 2874 4447 SmaI CCC'GGG Cuts at: 1872 2111 3606 4320 4648 1872 Size: 239 1495 714 328 4545 Fragments arranged by size: 4545 1495 714 328 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3194 3422 5425 6326 6588 Size: 197 926 543 708 228 2003 901 262 Cuts at: 6588 820 Size: 1553 Fragments arranged by size: 2003 1553 926 901 708 543 262 228 197 SnaBI TAC'GTA Cuts at: 493 5112 493 Size: 4619 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7321 SphI G_CATG'C Cuts at: 1132 4679 1132 Size: 3547 3774 SspI AAT'ATT Cuts at: 5 52 5245 5 Size: 47 5193 2081 Fragments arranged by size: 5193 2081 47 StuI AGG'CCT Cuts at: 1517 3018 3974 1517 Size: 1501 956 4864 Fragments arranged by size: 4864 1501 956 StyI C'CwwG_G Cuts at: 513 1065 2004 3360 3819 3900 4434 4760 513 Size: 552 939 1356 459 81 534 326 3074 Fragments arranged by size: 3074 1356 939 552 534 459 326 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3418 3605 3983 5348 5365 5518 5703 Size: 1255 1838 187 378 1365 17 153 185 Cuts at: 5703 6148 325 Size: 445 1498 Fragments arranged by size: 1838 1498 1365 1255 445 378 187 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2754 3087 Size: 274 80 33 51 1443 447 330 333 Cuts at: 3087 3117 3714 5567 7279 96 Size: 30 597 1853 1712 138 Fragments arranged by size: 1853 1712 1443 597 447 333 330 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4178 4210 7281 7313 4178 Size: 32 3071 32 4186 Fragments arranged by size: 4186 3071 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7321 XbaI T'CTAG_A Cuts at: 4657 4657 Size: 7321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 4378 4441 2548 Size: 1830 63 5428 Fragments arranged by size: 5428 1830 63 XhoI C'TCGA_G Cuts at: 1943 3422 1943 Size: 1479 5842 XmaI C'CCGG_G Cuts at: 1870 2109 3604 4318 4646 1870 Size: 239 1495 714 328 4545 Fragments arranged by size: 4545 1495 714 328 239 XmnI GAAnn'nnTTC Cuts at: 2504 4103 5450 2504 Size: 1599 1347 4375 Fragments arranged by size: 4375 1599 1347 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3703 5129 276 Size: 53 83 186 936 2169 1426 2468 Fragments arranged by size: 2468 2169 1426 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BfrBI BsiWI Bsu36I DraIII EcoRV FseI NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI