(Circular) (Six-base) MAPSORT of: pFN21AE1085.seq Check: 3035 from: 1 to: 7258 pFN21AE1085 7258 bp With 182 enzymes: SgfI * July 28, 2009 17:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2608 2074 Size: 534 6724 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2252 5068 278 Size: 53 83 186 936 716 2816 2468 Fragments arranged by size: 2816 2468 936 716 186 83 53 AccI GT'mk_AC Cuts at: 1937 4601 1937 Size: 2664 4594 Acc65I G'GTAC_C Cuts at: 4579 4579 Size: 7258 AclI AA'CG_TT Cuts at: 1563 5385 5758 1563 Size: 3822 373 3063 Fragments arranged by size: 3822 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4157 4163 5321 6705 6973 1632 Size: 2525 6 1158 1384 268 1917 Fragments arranged by size: 2525 1917 1384 1158 268 6 AflII C'TTAA_G Cuts at: 820 1017 2693 820 Size: 197 1676 5385 Fragments arranged by size: 5385 1676 197 AflIII A'CryG_T Cuts at: 1203 2185 6157 7134 1203 Size: 982 3972 977 1327 Fragments arranged by size: 3972 1327 982 977 AgeI A'CCGG_T Cuts at: 2533 6089 2533 Size: 3556 3702 AhdI GACnn_n'nnGTC Cuts at: 5987 5987 Size: 7258 AleI CACnn'nnGTG Cuts at: 1166 2534 3869 1166 Size: 1368 1335 4555 Fragments arranged by size: 4555 1368 1335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2043 1184 Size: 859 6399 AlwNI CAG_nnn'CTG Cuts at: 2017 2911 3044 3073 4060 5001 6573 2017 Size: 894 133 29 987 941 1572 2702 Fragments arranged by size: 2702 1572 987 941 894 133 29 ApaI G_GGCC'C Cuts at: 3067 3103 3429 3067 Size: 36 326 6896 Fragments arranged by size: 6896 326 36 ApaLI G'TGCA_C Cuts at: 5315 6471 5315 Size: 1156 6102 ApoI r'AATT_y Cuts at: 1493 4567 4793 4945 7054 1493 Size: 3074 226 152 2109 1697 Fragments arranged by size: 3074 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5812 160 Size: 872 4780 1606 Fragments arranged by size: 4780 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 AvaI C'yCGr_G Cuts at: 1870 1943 2408 3292 3699 3917 4583 1870 Size: 73 465 884 407 218 666 4545 Fragments arranged by size: 4545 884 666 465 407 218 73 BamHI G'GATC_C Cuts at: 4588 4955 4588 Size: 367 6891 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2536 2553 2585 2807 3047 Size: 325 217 627 749 17 32 222 240 Cuts at: 3047 3416 3933 4579 6034 618 Size: 369 517 646 1455 1842 Fragments arranged by size: 1842 1455 749 646 627 517 369 325 240 222 217 32 17 BanII G_rGCy'C Cuts at: 1391 2219 2444 2748 3067 3103 3131 3360 3375 Size: 828 225 304 319 36 28 229 15 Cuts at: 3375 3429 4535 4577 1391 Size: 54 1106 42 4072 Fragments arranged by size: 4072 1106 828 319 304 229 225 54 42 36 28 15 BbeI G_GCGC'C Cuts at: 3051 3051 Size: 7258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3273 4151 928 Size: 971 1374 878 4035 Fragments arranged by size: 4035 1374 971 878 BbvCI CC'TCA_GC Cuts at: 3095 3726 3095 Size: 631 6627 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5449 5483 1903 Size: 34 3512 34 3678 Fragments arranged by size: 3678 3512 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2980 5150 6360 2980 Size: 2170 1210 3878 Fragments arranged by size: 3878 2170 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2933 3118 3379 Size: 107 122 71 631 605 1261 185 261 Cuts at: 3379 3800 4214 4280 4289 5869 136 Size: 421 414 66 9 1580 1525 Fragments arranged by size: 1580 1525 1261 631 605 421 414 261 185 122 107 71 66 9 BglII A'GATC_T Cuts at: 7253 7253 Size: 7258 BlpI GC'TnA_GC Cuts at: 3773 4675 3773 Size: 902 6356 Bme1580I G_kGCm'C Cuts at: 1790 2556 2590 2810 3067 3103 3173 3180 3348 Size: 766 34 220 257 36 70 7 168 Cuts at: 3348 3419 3429 4389 5319 6475 1790 Size: 71 10 960 930 1156 2573 Fragments arranged by size: 2573 1156 960 930 766 257 220 168 71 70 36 34 10 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2448 2538 2774 Size: 416 53 83 399 24 1031 90 236 Cuts at: 2774 3167 4989 5947 442 Size: 393 1822 958 1753 Fragments arranged by size: 1822 1753 1031 958 416 399 393 236 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7258 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3325 3583 4446 3325 Size: 258 863 6137 Fragments arranged by size: 6137 863 258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2624 2630 2645 2810 2900 3095 3485 Size: 1127 258 6 15 165 90 195 390 Cuts at: 3485 3532 5918 1239 Size: 47 2386 2579 Fragments arranged by size: 2579 2386 1127 390 258 195 165 90 47 15 6 Bpu10I CC'TnA_GC Cuts at: 2213 2922 3095 3644 3726 2213 Size: 709 173 549 82 5745 Fragments arranged by size: 5745 709 549 173 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 2876 5383 6248 6546 2129 Size: 747 2507 865 298 2841 Fragments arranged by size: 2841 2507 865 747 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 2342 2786 2876 5921 882 Size: 617 603 240 444 90 3045 2219 Fragments arranged by size: 3045 2219 617 603 444 240 90 BsaAI yAC'GTr Cuts at: 493 1204 2023 2152 2566 3979 5049 493 Size: 711 819 129 414 1413 1070 2702 Fragments arranged by size: 2702 1413 1070 819 711 414 129 BsaBI GATnn'nnATC Cuts at: 3221 7252 3221 Size: 4031 3227 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2249 3048 5065 5447 Size: 53 83 186 936 716 799 2017 382 Cuts at: 5447 6082 275 Size: 635 1451 Fragments arranged by size: 2017 1451 936 799 716 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2533 3489 5691 6089 6363 6510 6921 2533 Size: 956 2202 398 274 147 411 2870 Fragments arranged by size: 2870 2202 956 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3152 3182 1178 Size: 30 165 30 1749 30 5254 Fragments arranged by size: 5254 1749 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 3419 3479 3508 3767 3770 1186 Size: 1441 792 60 29 259 3 4674 Fragments arranged by size: 4674 1441 792 259 60 29 3 BseYI C'CCAG_C Cuts at: 2053 2886 2943 3639 4388 6461 6957 2053 Size: 833 57 696 749 2073 496 2354 Fragments arranged by size: 2354 2073 833 749 696 496 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2003 2216 4001 4073 1163 Size: 573 267 213 1785 72 4348 Fragments arranged by size: 4348 1785 573 267 213 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2317 2463 4708 5469 6497 1540 Size: 275 186 316 146 2245 761 1028 2301 Fragments arranged by size: 2301 2245 1028 761 316 275 186 146 BsiHKAI G_wGCw'C Cuts at: 2219 2270 2344 2640 2661 2748 3335 4350 4427 Size: 51 74 296 21 87 587 1015 77 Cuts at: 4427 4505 5319 5404 6475 2219 Size: 78 814 85 1071 3002 Fragments arranged by size: 3002 1071 1015 814 587 296 87 85 78 77 74 51 21 BsmI GAATG_Cn' Cuts at: 4769 4862 4769 Size: 93 7165 BsmBI CGTCTCn'nnnn_ Cuts at: 2411 2411 Size: 7258 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2270 2344 2444 2556 2590 2640 Size: 399 429 51 74 100 112 34 50 Cuts at: 2640 2661 2748 2810 3067 3103 3131 3173 3180 Size: 21 87 62 257 36 28 42 7 Cuts at: 3180 3335 3348 3360 3375 3419 3429 4350 4389 Size: 155 13 12 15 44 10 921 39 Cuts at: 4389 4427 4505 4535 4577 5319 5404 6475 1391 Size: 38 78 30 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 921 742 429 399 257 155 112 100 87 85 78 74 62 51 50 44 42 42 39 38 36 34 30 28 21 15 13 12 10 7 BspEI T'CCGG_A Cuts at: 6921 6921 Size: 7258 BspHI T'CATG_A Cuts at: 3450 5147 6877 3450 Size: 1697 1730 3831 Fragments arranged by size: 3831 1730 1697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2608 4613 844 Size: 1230 534 2005 3489 Fragments arranged by size: 3489 2005 1230 534 BsrBI CCG'CTC Cuts at: 1394 1823 3844 5145 1394 Size: 429 2021 1301 3507 Fragments arranged by size: 3507 2021 1301 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 5753 5927 65 Size: 1183 1866 2639 174 1396 Fragments arranged by size: 2639 1866 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2432 2462 2533 3419 3678 5902 6089 Size: 110 622 30 71 886 259 2224 187 Cuts at: 6089 1700 Size: 2869 Fragments arranged by size: 2869 2224 886 622 259 187 110 71 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7258 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7258 BssSI C'ACGA_G Cuts at: 5318 6330 5318 Size: 1012 6246 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 4060 794 Size: 2117 1149 3992 Fragments arranged by size: 3992 2117 1149 BstBI TT'CG_AA Cuts at: 6062 6062 Size: 7258 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7258 BstXI CCAn_nnnn'nTGG Cuts at: 2437 2437 Size: 7258 BstYI r'GATC_y Cuts at: 1972 4588 4955 5340 5357 6809 6895 6907 7253 Size: 2616 367 385 17 1452 86 12 346 Cuts at: 7253 1972 Size: 1977 Fragments arranged by size: 2616 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 2227 2317 2353 2369 2510 Size: 552 939 99 124 90 36 16 141 Cuts at: 2510 3011 3480 4047 4249 4323 513 Size: 501 469 567 202 74 3448 Fragments arranged by size: 3448 939 567 552 501 469 202 141 124 99 90 74 36 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3255 3973 4431 487 Size: 683 56 2029 718 458 3314 Fragments arranged by size: 3314 2029 718 683 458 56 BtsI GCAGTG_nn' Cuts at: 2199 3720 4774 5568 5588 2199 Size: 1521 1054 794 20 3869 Fragments arranged by size: 3869 1521 1054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7258 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2260 2295 529 Size: 35 1696 35 5492 Fragments arranged by size: 5492 1696 35 35 DraI TTT'AAA Cuts at: 4562 4562 Size: 7258 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2975 3980 4073 6265 809 Size: 507 1659 1005 93 2192 1802 Fragments arranged by size: 2192 1802 1659 1005 507 93 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2421 2460 Size: 54 1425 50 275 18 484 107 39 Cuts at: 2460 2957 3030 3627 3666 4134 4239 4351 4380 Size: 497 73 597 39 468 105 112 29 Cuts at: 4380 4705 5594 7223 8 Size: 325 889 1629 43 Fragments arranged by size: 1629 1425 889 597 497 484 468 325 275 112 107 105 73 54 50 43 39 39 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2314 2460 4705 1537 Size: 275 502 146 2245 4090 Fragments arranged by size: 4090 2245 502 275 146 EarI CTCTTCn'nnn_ Cuts at: 1353 3323 4184 4548 5188 1353 Size: 1970 861 364 640 3423 Fragments arranged by size: 3423 1970 861 640 364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5845 6219 6365 387 Size: 1064 138 4256 374 146 1280 Fragments arranged by size: 4256 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2217 2746 2217 Size: 529 6729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2624 2630 2645 2810 2900 3095 Size: 393 734 258 6 15 165 90 195 Cuts at: 3095 3485 3532 4157 4163 5321 5918 6705 6973 Size: 390 47 625 6 1158 597 787 268 Cuts at: 6973 1239 Size: 1524 Fragments arranged by size: 1524 1158 787 734 625 597 393 390 268 258 195 165 90 47 15 6 6 EcoO109I rG'GnC_Cy Cuts at: 2014 2043 2083 2776 3099 3100 3426 3595 2014 Size: 29 40 693 323 1 326 169 5677 Fragments arranged by size: 5677 693 326 323 169 40 29 1 EcoRI G'AATT_C Cuts at: 4567 4945 4567 Size: 378 6880 FspI TGC'GCA Cuts at: 5764 5764 Size: 7258 HaeII r_GCGC'y Cuts at: 1678 2203 2903 3051 6405 1678 Size: 525 700 148 3354 2531 Fragments arranged by size: 3354 2531 700 525 148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3521 3553 3719 3751 3773 Size: 32 135 32 1949 32 166 32 22 Cuts at: 3773 3805 4304 4336 4454 4486 5899 5931 5973 Size: 32 499 32 118 32 1413 32 42 Cuts at: 5973 6005 1373 Size: 32 2626 Fragments arranged by size: 2626 1949 1413 499 166 135 118 42 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3517 3985 4300 4602 4848 669 Size: 988 281 1579 468 315 302 246 3079 Fragments arranged by size: 3079 1579 988 468 315 302 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7258 HpaI GTT'AAC Cuts at: 4848 4848 Size: 7258 KasI G'GCGC_C Cuts at: 3047 3047 Size: 7258 KpnI G_GTAC'C Cuts at: 4583 4583 Size: 7258 MfeI C'AATT_G Cuts at: 4857 4857 Size: 7258 MluI A'CGCG_T Cuts at: 2185 7134 2185 Size: 4949 2309 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6372 6556 1305 Size: 5067 184 2007 Fragments arranged by size: 5067 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2959 3032 4136 10 Size: 54 1425 343 1127 73 1104 3132 Fragments arranged by size: 3132 1425 1127 1104 343 73 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 3869 5216 5575 5734 518 Size: 648 1368 1335 1347 359 159 2042 Fragments arranged by size: 2042 1368 1347 1335 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2355 2371 2608 2726 3044 3061 3182 3665 Size: 749 16 237 118 318 17 121 483 Cuts at: 3665 3694 4060 4071 4392 4674 5351 6499 6744 Size: 29 366 11 321 282 677 1148 245 Cuts at: 6744 1606 Size: 2120 Fragments arranged by size: 2120 1148 749 677 483 366 321 318 282 245 237 121 118 29 17 16 11 NaeI GCC'GGC Cuts at: 2464 3680 2464 Size: 1216 6042 NarI GG'CG_CC Cuts at: 3048 3048 Size: 7258 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5767 Fragments arranged by size: 5767 939 552 NdeI CA'TA_TG Cuts at: 387 3204 387 Size: 2817 4441 NgoMIV G'CCGG_C Cuts at: 2462 3678 2462 Size: 1216 6042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7258 NotI GC'GGCC_GC Cuts at: 4705 4705 Size: 7258 NruI TCG'CGA Cuts at: 4416 4416 Size: 7258 NspI r_CATG'y Cuts at: 1132 1229 2839 4616 6161 1132 Size: 97 1610 1777 1545 2229 Fragments arranged by size: 2229 1777 1610 1545 97 PasI CC'CwG_GG Cuts at: 1348 2039 1348 Size: 691 6567 PciI A'CATG_T Cuts at: 6157 6157 Size: 7258 PflMI CCAn_nnn'nTGG Cuts at: 1108 3073 3154 3508 1108 Size: 1965 81 354 4858 Fragments arranged by size: 4858 1965 354 81 PfoI T'CCnGG_A Cuts at: 2661 2661 Size: 7258 PmeI GTTT'AAAC Cuts at: 4562 4562 Size: 7258 PmlI CAC'GTG Cuts at: 2023 2566 2023 Size: 543 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5327 5359 6840 6872 5327 Size: 32 1481 32 5713 Fragments arranged by size: 5713 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2014 2043 2083 2776 2014 Size: 29 40 693 6496 Fragments arranged by size: 6496 693 40 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7258 PsiI TTA'TAA Cuts at: 4828 4828 Size: 7258 PspOMI G'GGCC_C Cuts at: 3063 3099 3425 3063 Size: 36 326 6896 Fragments arranged by size: 6896 326 36 PstI C_TGCA'G Cuts at: 830 2907 2916 3187 3655 3879 4610 830 Size: 2077 9 271 468 224 731 3478 Fragments arranged by size: 3478 2077 731 468 271 224 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7258 PvuII CAG'CTG Cuts at: 2608 3694 4060 4392 2608 Size: 1086 366 332 5474 Fragments arranged by size: 5474 1086 366 332 RsrII CG'GwC_CG Cuts at: 2977 2977 Size: 7258 SacI G_AGCT'C Cuts at: 2219 2748 2219 Size: 529 6729 SacII CC_GC'GG Cuts at: 2356 2372 2356 Size: 16 7242 SalI G'TCGA_C Cuts at: 1936 4600 1936 Size: 2664 4594 SanDI GG'GwC_CC Cuts at: 2043 2043 Size: 7258 SapI GCTCTTCn'nnn_ Cuts at: 3323 4184 3323 Size: 861 6397 SbfI CC_TGCA'GG Cuts at: 3655 4610 3655 Size: 955 6303 ScaI AGT'ACT Cuts at: 5506 7218 5506 Size: 1712 5546 SexAI A'CCwGG_T Cuts at: 3936 3936 Size: 7258 SfcI C'TryA_G Cuts at: 826 1045 2903 2912 3183 3651 3875 4108 4606 Size: 219 1858 9 271 468 224 233 498 Cuts at: 4606 5741 6422 6613 826 Size: 1135 681 191 1471 Fragments arranged by size: 1858 1471 1135 681 498 468 271 233 224 219 191 9 SfoI GGC'GCC Cuts at: 3049 3049 Size: 7258 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7258 SgrAI Cr'CCGG_yG Cuts at: 1700 2533 3419 1700 Size: 833 886 5539 Fragments arranged by size: 5539 886 833 SmaI CCC'GGG Cuts at: 1872 3294 4585 1872 Size: 1422 1291 4545 Fragments arranged by size: 4545 1422 1291 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 2408 2693 2891 3699 5362 Size: 197 926 201 264 285 198 808 1663 Cuts at: 5362 6263 6525 820 Size: 901 262 1553 Fragments arranged by size: 1663 1553 926 901 808 285 264 262 201 198 197 SnaBI TAC'GTA Cuts at: 493 5049 493 Size: 4556 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7258 SphI G_CATG'C Cuts at: 1132 2839 4616 1132 Size: 1707 1777 3774 Fragments arranged by size: 3774 1777 1707 SspI AAT'ATT Cuts at: 5 52 5182 5 Size: 47 5130 2081 Fragments arranged by size: 5130 2081 47 StuI AGG'CCT Cuts at: 1517 2954 3121 3255 3649 1517 Size: 1437 167 134 394 5126 Fragments arranged by size: 5126 1437 394 167 134 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2865 4443 4697 513 Size: 552 939 75 786 1578 254 3074 Fragments arranged by size: 3074 1578 939 786 552 254 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 3365 3377 5285 5302 5455 5640 Size: 1255 863 922 12 1908 17 153 185 Cuts at: 5640 6085 325 Size: 445 1498 Fragments arranged by size: 1908 1498 1255 922 863 445 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2686 5504 7216 Size: 274 80 33 51 1443 709 2818 1712 Cuts at: 7216 96 Size: 138 Fragments arranged by size: 2818 1712 1443 709 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 3123 3152 3155 3184 7218 7250 2055 Size: 32 1036 29 3 29 4034 32 2063 Fragments arranged by size: 4034 2063 1036 32 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 4265 1316 Size: 2949 4309 XbaI T'CTAG_A Cuts at: 4594 4594 Size: 7258 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4045 4045 Size: 7258 XhoI C'TCGA_G Cuts at: 1943 2408 3699 1943 Size: 465 1291 5502 Fragments arranged by size: 5502 1291 465 XmaI C'CCGG_G Cuts at: 1870 3292 4583 1870 Size: 1422 1291 4545 Fragments arranged by size: 4545 1422 1291 XmnI GAAnn'nnTTC Cuts at: 5387 5387 Size: 7258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2250 5066 276 Size: 53 83 186 936 716 2816 2468 Fragments arranged by size: 2816 2468 936 716 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PspXI PsrI SfiI SrfI SwaI