Restriction map for pFN21AA1082
##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
BamHI       BbrPI       BciVI       BfrI        BsaAI       
BsiWI       BsmBI       BspDI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       HpaI        MluI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SwaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII       2825  TCCGGA               T^CCGGA
AcyI          294  GGCGCC               G[GA]^CG[CT]C
AflIII       5124  ACATGT               A^C[GA][CT]GT
AhdI         3407  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3726  CCTGAGG              CC^T[ACGT]AGG
AspEI        3407  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          307  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         125  TGCGCA               TGC^GCA
AvrII        5028  CCTAGG               C^CTAGG
BbeI          294  GGCGCC               GGCGC^C
BglI          136  GCCTGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         5028  CCTAGG               C^CTAGG
BsaBI        4722  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         294  GGCGCC               G[GA]^CG[CT]C
BseAI        2825  TCCGGA               T^CCGGA
BsiEI        1360  CGGTCG               CG[GA][CT]^CG
Bsp1407I     4619  TGTACA               T^GTACA
BspEI        2825  TCCGGA               T^CCGGA
BspLU11I     5124  ACATGT               A^CATGT
BsrGI        4619  TGTACA               T^GTACA
Bsu36I       3726  CCTGAGG              CC^T[ACGT]AGG
DraIII       3191  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3407  GACCTGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3726  CCTGAGG              CC^T[ACGT]AGG
FseI         2602  GGCCGGCC             GGCCGG^CC
FspI          125  TGCGCA               TGC^GCA
HaeII         294  GGCGCC               [GA]GCGC^[CT]
HgaI            7  GACGC                GACGC(5/10)
Hin1I         294  GGCGCC               G[GA]^CG[CT]C
HincII       3383  GTCGAC               GT[CT]^[GA]AC
HindII       3383  GTCGAC               GT[CT]^[GA]AC
KasI          294  GGCGCC               G^GCGCC
KspI           86  CCGCGG               CCGC^GG
MamI         4722  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2825  TCCGGA               T^CCGGA
NarI          294  GGCGCC               GG^CGCC
NcoI         2156  CCATGG               C^CATGG
NdeI         4404  CATATG               CA^TATG
PaeR7I        131  CTCGAG               C^TCGAG
PflFI         307  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3045  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4415  GACTACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII          86  CCGCGG               CCGC^GG
SalI         3383  GTCGAC               G^TCGAC
SbfI         2694  CCTGCAGG             CCTGCA^GG
SfoI          294  GGCGCC               GGC^GCC
SmaI         3341  CCCGGG               CCC^GGG
Sse8387I     2694  CCTGCAGG             CCTGCA^GG
SspBI        4619  TGTACA               T^GTACA
SspI         3316  AATATT               AAT^ATT
Tth111I       307  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3045  CCAGAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2949  TCTAGA               T^CTAGA
XhoI          131  CTCGAG               C^TCGAG
XmaCI        3341  CCCGGG               C^CCGGG
XmaI         3341  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I       2762  GGTACC               G^GTACC
             4955  GGTACC              
Asp718I      2762  GGTACC               G^GTACC
             4955  GGTACC              
BbsI         5104  GAAGAC               GAAGAC(2/6)
             3952  GTCTTC              
BcgI         4773  CGAGGGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2733  GCACAAGTGTCG        
BclI          542  TGATCA               T^GATCA
             4926  TGATCA              
BlpI         2120  GCTCAGC              GC^T[ACGT]AGC
             2557  GCTGAGC             
Bpu1102I     2120  GCTCAGC              GC^T[ACGT]AGC
             2557  GCTGAGC             
BpuAI        5104  GAAGAC               GAAGAC(2/6)
             3952  GTCTTC              
BsrDI         339  CATTGC               GCAATG(2/0)
             4017  CATTGC              
CelII        2120  GCTCAGC              GC^T[ACGT]AGC
             2557  GCTGAGC             
EcoNI         315  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5145  CCTGACTCAGG         
EcoT22I      1584  ATGCAT               ATGCA^T
             2709  ATGCAT              
FbaI          542  TGATCA               T^GATCA
             4926  TGATCA              
HindIII      2406  AAGCTT               A^AGCTT
             3814  AAGCTT              
KpnI         2762  GGTACC               GGTAC^C
             4955  GGTACC              
MaeII         787  ACGT                 A^CGT
             2300  ACGT                
MfeI         4200  CAATTG               C^AATTG
             4766  CAATTG              
MunI         4200  CAATTG               C^AATTG
             4766  CAATTG              
NsiI         1584  ATGCAT               ATGCA^T
             2709  ATGCAT              
SacI         1692  GAGCTC               GAGCT^C
             4279  GAGCTC              
SexAI        4498  ACCAGGT              A^CC[AT]GGT
             5066  ACCAGGT             
XcmI         3023  CCATGATGATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4828  CCAGGTGCTTTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvCI       BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspHI       BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI        
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