Restriction map for
pFN21AB5610
(Linear) (Six-base) MAPSORT of: KIBB5610.seq Check: 4204 from: 1 to: 3036
KIBB5610.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 0 1743 3036
Size: 1743 1293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 2306 2356 2435 2761 3036
Size: 932 1374 50 79 326 275
Fragments arranged by size:
1374 932 326 275 79 50
AflIII A'CryG_T
Cuts at: 0 761 3036
Size: 761 2275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 815 1184 2377 3036
Size: 815 369 1193 659
Fragments arranged by size:
1193 815 659 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 751 783 3036
Size: 751 32 2253
Fragments arranged by size:
2253 751 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1687 3036
Size: 1687 1349
ApaLI G'TGCA_C
Cuts at: 0 2083 3036
Size: 2083 953
ApoI r'AATT_y
Cuts at: 0 301 349 401 1621 2116 3036
Size: 301 48 52 1220 495 920
Fragments arranged by size:
1220 920 495 301 52 48
AseI AT'TA_AT
Cuts at: 0 836 3036
Size: 836 2200
AvaI C'yCGr_G
Cuts at: 0 60 130 2223 3036
Size: 60 70 2093 813
Fragments arranged by size:
2093 813 70 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2096 2129 3036
Size: 2096 33 907
Fragments arranged by size:
2096 907 33
BanI G'GyrC_C
Cuts at: 0 287 1891 3036
Size: 287 1604 1145
Fragments arranged by size:
1604 1145 287
BanII G_rGCy'C
Cuts at: 0 210 1910 3036
Size: 210 1700 1126
Fragments arranged by size:
1700 1126 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 266 1781 2062 3036
Size: 173 93 1515 281 974
Fragments arranged by size:
1515 974 281 173 93
BciVI GTATCCnnnnn_n'
Cuts at: 0 2097 3036
Size: 2097 939
BclI T'GATC_A
Cuts at: 0 1818 2232 2879 3036
Size: 1818 414 647 157
Fragments arranged by size:
1818 647 414 157
BglII A'GATC_T
Cuts at: 0 1026 1829 2483 3036
Size: 1026 803 654 553
Fragments arranged by size:
1026 803 654 553
BlpI GC'TnA_GC
Cuts at: 0 1904 3036
Size: 1904 1132
Bme1580I G_kGCm'C
Cuts at: 0 173 417 2087 3036
Size: 173 244 1670 949
Fragments arranged by size:
1670 949 244 173
BmgBI CAC'GTC
Cuts at: 0 102 523 3036
Size: 102 421 2513
Fragments arranged by size:
2513 421 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 2158 2268 3036
Size: 2158 110 768
Fragments arranged by size:
2158 768 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 892 1651 3036
Size: 892 759 1385
Fragments arranged by size:
1385 892 759
Bpu10I CC'TnA_GC
Cuts at: 0 1968 3036
Size: 1968 1068
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 2957 3036
Size: 1471 1486 79
Fragments arranged by size:
1486 1471 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 930 3036
Size: 930 2106
BsaAI yAC'GTr
Cuts at: 0 2676 3036
Size: 2676 360
BsaBI GATnn'nnATC
Cuts at: 0 1817 3036
Size: 1817 1219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 239 269 365 395 751 781 3036
Size: 239 30 96 30 356 30 2255
Fragments arranged by size:
2255 356 239 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2231 3036
Size: 2231 805
BseYI C'CCAG_C
Cuts at: 0 172 227 3036
Size: 172 55 2809
Fragments arranged by size:
2809 172 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 2430 3036
Size: 602 1828 606
Fragments arranged by size:
1828 606 602
BsiHKAI G_wGCw'C
Cuts at: 0 210 873 1515 1910 2087 2267 3036
Size: 210 663 642 395 177 180 769
Fragments arranged by size:
769 663 642 395 210 180 177
BsmI GAATG_Cn'
Cuts at: 0 2843 3036
Size: 2843 193
Bsp1286I G_dGCh'C
Cuts at: 0 173 210 417 873 1515 1910 2087 2267
Size: 173 37 207 456 642 395 177 180
Cuts at: 2267 3036
Size: 769
Fragments arranged by size:
769 642 456 395 207 180 177 173
37
BspHI T'CATG_A
Cuts at: 0 3006 3036
Size: 3006 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 243 912 1369 1520 3036
Size: 243 669 457 151 1516
Fragments arranged by size:
1516 669 457 243 151
BsrBI CCG'CTC
Cuts at: 0 2538 3036
Size: 2538 498
BsrDI GCAATG_nn'
Cuts at: 0 2366 3036
Size: 2366 670
BsrFI r'CCGG_y
Cuts at: 0 284 3036
Size: 284 2752
BsrGI T'GTAC_A
Cuts at: 0 2936 3036
Size: 2936 100
BssSI C'ACGA_G
Cuts at: 0 199 3036
Size: 199 2837
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1063 3036
Size: 1063 1973
BstEII G'GTnAC_C
Cuts at: 0 2820 3036
Size: 2820 216
BstYI r'GATC_y
Cuts at: 0 1026 1829 2483 2562 3036
Size: 1026 803 654 79 474
Fragments arranged by size:
1026 803 654 474 79
BtgI C'CryG_G
Cuts at: 0 13 39 155 3036
Size: 13 26 116 2881
Fragments arranged by size:
2881 116 26 13
BtsI GCAGTG_nn'
Cuts at: 0 1011 1168 1366 1588 1924 3036
Size: 1011 157 198 222 336 1112
Fragments arranged by size:
1112 1011 336 222 198 157
DraI TTT'AAA
Cuts at: 0 1343 2955 3036
Size: 1343 1612 81
Fragments arranged by size:
1612 1343 81
EaeI y'GGCC_r
Cuts at: 0 2965 3036
Size: 2965 71
EarI CTCTTCn'nnn_
Cuts at: 0 883 3036
Size: 883 2153
EcoICRI GAG'CTC
Cuts at: 0 208 1908 3036
Size: 208 1700 1128
Fragments arranged by size:
1700 1128 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 2306 2356 2435 2761 3036
Size: 932 1374 50 79 326 275
Fragments arranged by size:
1374 932 326 275 79 50
EcoO109I rG'GnC_Cy
Cuts at: 0 255 1532 3036
Size: 255 1277 1504
Fragments arranged by size:
1504 1277 255
EcoRV GAT'ATC
Cuts at: 0 1237 2802 3036
Size: 1237 1565 234
Fragments arranged by size:
1565 1237 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1456 2203 3036
Size: 1456 747 833
Fragments arranged by size:
1456 833 747
HaeII r_GCGC'y
Cuts at: 0 2857 3036
Size: 2857 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 239 271 877 909 971 1003 1286 1318
Size: 239 32 606 32 62 32 283 32
Cuts at: 1318 1587 1619 2217 2249 3036
Size: 269 32 598 32 787
Fragments arranged by size:
787 606 598 283 269 239 62 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1744 3036
Size: 1744 1292
HindIII A'AGCT_T
Cuts at: 0 1188 3036
Size: 1188 1848
MfeI C'AATT_G
Cuts at: 0 296 1058 2792 3036
Size: 296 762 1734 244
Fragments arranged by size:
1734 762 296 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 645 1649 3036
Size: 444 201 1004 1387
Fragments arranged by size:
1387 1004 444 201
MslI CAynn'nnrTG
Cuts at: 0 2318 2688 2863 3011 3036
Size: 2318 370 175 148 25
Fragments arranged by size:
2318 370 175 148 25
MspA1I CmG'CkG
Cuts at: 0 15 41 1684 3036
Size: 15 26 1643 1352
Fragments arranged by size:
1643 1352 26 15
NdeI CA'TA_TG
Cuts at: 0 1071 3036
Size: 1071 1965
NspI r_CATG'y
Cuts at: 0 473 765 1182 3036
Size: 473 292 417 1854
Fragments arranged by size:
1854 473 417 292
PciI A'CATG_T
Cuts at: 0 761 3036
Size: 761 2275
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 1192 3036
Size: 915 277 1844
Fragments arranged by size:
1844 915 277
PfoI T'CCnGG_A
Cuts at: 0 184 3036
Size: 184 2852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 3036
Size: 751 32 2253
Fragments arranged by size:
2253 751 32
PpuMI rG'GwC_Cy
Cuts at: 0 255 1532 3036
Size: 255 1277 1504
Fragments arranged by size:
1504 1277 255
PsiI TTA'TAA
Cuts at: 0 2780 3036
Size: 2780 256
PstI C_TGCA'G
Cuts at: 0 1931 2205 3036
Size: 1931 274 831
Fragments arranged by size:
1931 831 274
PvuII CAG'CTG
Cuts at: 0 1684 3036
Size: 1684 1352
SacI G_AGCT'C
Cuts at: 0 210 1910 3036
Size: 210 1700 1126
Fragments arranged by size:
1700 1126 210
SacII CC_GC'GG
Cuts at: 0 16 42 3036
Size: 16 26 2994
Fragments arranged by size:
2994 26 16
SalI G'TCGA_C
Cuts at: 0 1742 3036
Size: 1742 1294
ScaI AGT'ACT
Cuts at: 0 121 1439 3036
Size: 121 1318 1597
Fragments arranged by size:
1597 1318 121
SfcI C'TryA_G
Cuts at: 0 818 937 1524 1808 1927 2201 3036
Size: 818 119 587 284 119 274 835
Fragments arranged by size:
835 818 587 284 274 119 119
SgrAI Cr'CCGG_yG
Cuts at: 0 284 3036
Size: 284 2752
SmlI C'TyrA_G
Cuts at: 0 1486 2223 2972 3036
Size: 1486 737 749 64
Fragments arranged by size:
1486 749 737 64
SnaBI TAC'GTA
Cuts at: 0 2676 3036
Size: 2676 360
SphI G_CATG'C
Cuts at: 0 1182 3036
Size: 1182 1854
StyI C'CwwG_G
Cuts at: 0 332 2028 3036
Size: 332 1696 1008
Fragments arranged by size:
1696 1008 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 241 2865 3036
Size: 22 219 2624 171
Fragments arranged by size:
2624 219 171 22
TatI w'GTAC_w
Cuts at: 0 119 1437 2936 3036
Size: 119 1318 1499 100
Fragments arranged by size:
1499 1318 119 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 3036
Size: 365 32 2639
Fragments arranged by size:
2639 365 32
Tth111I GACn'n_nGTC
Cuts at: 0 1780 3036
Size: 1780 1256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 425 1017 2814 3036
Size: 367 58 592 1797 222
Fragments arranged by size:
1797 592 367 222 58
XhoI C'TCGA_G
Cuts at: 0 2223 3036
Size: 2223 813
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstYI BtgI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII MfeI MmeI MslI MspA1I NdeI NspI
PciI PflMI PfoI PpiI PpuMI PsiI PstI PvuII
SacI SacII SalI ScaI SfcI SgrAI SmlI SnaBI
SphI StyI TaqII TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI AscI AsiSI AvrII BamHI BbeI BbvCI
BcgI BfrBI BglI BmtI BpmI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BssHII BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoRI FseI FspI FspAI HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SanDI SapI SbfI SexAI SfiI
SfoI SgfI SmaI SpeI SrfI SspI StuI SwaI
XbaI XmaI XmnI ZraI