Restriction map for pFN21AB5610
 (Linear) (Six-base) MAPSORT of: KIBB5610.seq  Check: 4204  from: 1  to: 3036



KIBB5610.orf

 With 182 enzymes: SgfI * 

                        January 7, 2013 14:38  ..

AccI GT'mk_AC

Cuts at:      0    1743    3036 
   Size:       1743    1293
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     932    2306    2356    2435    2761    3036 
   Size:        932    1374      50      79     326     275

  Fragments arranged by size: 

                1374     932     326     275      79      50
 
AflIII A'CryG_T

Cuts at:      0     761    3036 
   Size:        761    2275
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     815    1184    2377    3036 
   Size:        815     369    1193     659

  Fragments arranged by size: 

                1193     815     659     369
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     751     783    3036 
   Size:        751      32    2253

  Fragments arranged by size: 

                2253     751      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1687    3036 
   Size:       1687    1349
 
ApaLI G'TGCA_C

Cuts at:      0    2083    3036 
   Size:       2083     953
 
ApoI r'AATT_y

Cuts at:      0     301     349     401    1621    2116    3036 
   Size:        301      48      52    1220     495     920

  Fragments arranged by size: 

                1220     920     495     301      52      48
 
AseI AT'TA_AT

Cuts at:      0     836    3036 
   Size:        836    2200
 
AvaI C'yCGr_G

Cuts at:      0      60     130    2223    3036 
   Size:         60      70    2093     813

  Fragments arranged by size: 

                2093     813      70      60
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2096    2129    3036 
   Size:       2096      33     907

  Fragments arranged by size: 

                2096     907      33
 
BanI G'GyrC_C

Cuts at:      0     287    1891    3036 
   Size:        287    1604    1145

  Fragments arranged by size: 

                1604    1145     287
 
BanII G_rGCy'C

Cuts at:      0     210    1910    3036 
   Size:        210    1700    1126

  Fragments arranged by size: 

                1700    1126     210
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     266    1781    2062    3036 
   Size:        173      93    1515     281     974

  Fragments arranged by size: 

                1515     974     281     173      93
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2097    3036 
   Size:       2097     939
 
BclI T'GATC_A

Cuts at:      0    1818    2232    2879    3036 
   Size:       1818     414     647     157

  Fragments arranged by size: 

                1818     647     414     157
 
BglII A'GATC_T

Cuts at:      0    1026    1829    2483    3036 
   Size:       1026     803     654     553

  Fragments arranged by size: 

                1026     803     654     553
 
BlpI GC'TnA_GC

Cuts at:      0    1904    3036 
   Size:       1904    1132
 
Bme1580I G_kGCm'C

Cuts at:      0     173     417    2087    3036 
   Size:        173     244    1670     949

  Fragments arranged by size: 

                1670     949     244     173
 
BmgBI CAC'GTC

Cuts at:      0     102     523    3036 
   Size:        102     421    2513

  Fragments arranged by size: 

                2513     421     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2158    2268    3036 
   Size:       2158     110     768

  Fragments arranged by size: 

                2158     768     110
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     892    1651    3036 
   Size:        892     759    1385

  Fragments arranged by size: 

                1385     892     759
 
Bpu10I CC'TnA_GC

Cuts at:      0    1968    3036 
   Size:       1968    1068
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1471    2957    3036 
   Size:       1471    1486      79

  Fragments arranged by size: 

                1486    1471      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     930    3036 
   Size:        930    2106
 
BsaAI yAC'GTr

Cuts at:      0    2676    3036 
   Size:       2676     360
 
BsaBI GATnn'nnATC

Cuts at:      0    1817    3036 
   Size:       1817    1219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     239     269     365     395     751     781    3036 
   Size:        239      30      96      30     356      30    2255

  Fragments arranged by size: 

                2255     356     239      96      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2231    3036 
   Size:       2231     805
 
BseYI C'CCAG_C

Cuts at:      0     172     227    3036 
   Size:        172      55    2809

  Fragments arranged by size: 

                2809     172      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602    2430    3036 
   Size:        602    1828     606

  Fragments arranged by size: 

                1828     606     602
 
BsiHKAI G_wGCw'C

Cuts at:      0     210     873    1515    1910    2087    2267    3036 
   Size:        210     663     642     395     177     180     769

  Fragments arranged by size: 

                 769     663     642     395     210     180     177
 
BsmI GAATG_Cn'

Cuts at:      0    2843    3036 
   Size:       2843     193
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     210     417     873    1515    1910    2087    2267 
   Size:        173      37     207     456     642     395     177     180

Cuts at:   2267    3036 
   Size:        769

  Fragments arranged by size: 

                 769     642     456     395     207     180     177     173
                  37
 
BspHI T'CATG_A

Cuts at:      0    3006    3036 
   Size:       3006      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     243     912    1369    1520    3036 
   Size:        243     669     457     151    1516

  Fragments arranged by size: 

                1516     669     457     243     151
 
BsrBI CCG'CTC

Cuts at:      0    2538    3036 
   Size:       2538     498
 
BsrDI GCAATG_nn'

Cuts at:      0    2366    3036 
   Size:       2366     670
 
BsrFI r'CCGG_y

Cuts at:      0     284    3036 
   Size:        284    2752
 
BsrGI T'GTAC_A

Cuts at:      0    2936    3036 
   Size:       2936     100
 
BssSI C'ACGA_G

Cuts at:      0     199    3036 
   Size:        199    2837
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1063    3036 
   Size:       1063    1973
 
BstEII G'GTnAC_C

Cuts at:      0    2820    3036 
   Size:       2820     216
 
BstYI r'GATC_y

Cuts at:      0    1026    1829    2483    2562    3036 
   Size:       1026     803     654      79     474

  Fragments arranged by size: 

                1026     803     654     474      79
 
BtgI C'CryG_G

Cuts at:      0      13      39     155    3036 
   Size:         13      26     116    2881

  Fragments arranged by size: 

                2881     116      26      13
 
BtsI GCAGTG_nn'

Cuts at:      0    1011    1168    1366    1588    1924    3036 
   Size:       1011     157     198     222     336    1112

  Fragments arranged by size: 

                1112    1011     336     222     198     157
 
DraI TTT'AAA

Cuts at:      0    1343    2955    3036 
   Size:       1343    1612      81

  Fragments arranged by size: 

                1612    1343      81
 
EaeI y'GGCC_r

Cuts at:      0    2965    3036 
   Size:       2965      71
 
EarI CTCTTCn'nnn_

Cuts at:      0     883    3036 
   Size:        883    2153
 
EcoICRI GAG'CTC

Cuts at:      0     208    1908    3036 
   Size:        208    1700    1128

  Fragments arranged by size: 

                1700    1128     208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     932    2306    2356    2435    2761    3036 
   Size:        932    1374      50      79     326     275

  Fragments arranged by size: 

                1374     932     326     275      79      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255    1532    3036 
   Size:        255    1277    1504

  Fragments arranged by size: 

                1504    1277     255
 
EcoRV GAT'ATC

Cuts at:      0    1237    2802    3036 
   Size:       1237    1565     234

  Fragments arranged by size: 

                1565    1237     234
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1456    2203    3036 
   Size:       1456     747     833

  Fragments arranged by size: 

                1456     833     747
 
HaeII r_GCGC'y

Cuts at:      0    2857    3036 
   Size:       2857     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271     877     909     971    1003    1286    1318 
   Size:        239      32     606      32      62      32     283      32

Cuts at:   1318    1587    1619    2217    2249    3036 
   Size:        269      32     598      32     787

  Fragments arranged by size: 

                 787     606     598     283     269     239      62      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1744    3036 
   Size:       1744    1292
 
HindIII A'AGCT_T

Cuts at:      0    1188    3036 
   Size:       1188    1848
 
MfeI C'AATT_G

Cuts at:      0     296    1058    2792    3036 
   Size:        296     762    1734     244

  Fragments arranged by size: 

                1734     762     296     244
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     645    1649    3036 
   Size:        444     201    1004    1387

  Fragments arranged by size: 

                1387    1004     444     201
 
MslI CAynn'nnrTG

Cuts at:      0    2318    2688    2863    3011    3036 
   Size:       2318     370     175     148      25

  Fragments arranged by size: 

                2318     370     175     148      25
 
MspA1I CmG'CkG

Cuts at:      0      15      41    1684    3036 
   Size:         15      26    1643    1352

  Fragments arranged by size: 

                1643    1352      26      15
 
NdeI CA'TA_TG

Cuts at:      0    1071    3036 
   Size:       1071    1965
 
NspI r_CATG'y

Cuts at:      0     473     765    1182    3036 
   Size:        473     292     417    1854

  Fragments arranged by size: 

                1854     473     417     292
 
PciI A'CATG_T

Cuts at:      0     761    3036 
   Size:        761    2275
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     915    1192    3036 
   Size:        915     277    1844

  Fragments arranged by size: 

                1844     915     277
 
PfoI T'CCnGG_A

Cuts at:      0     184    3036 
   Size:        184    2852
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     751     783    3036 
   Size:        751      32    2253

  Fragments arranged by size: 

                2253     751      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     255    1532    3036 
   Size:        255    1277    1504

  Fragments arranged by size: 

                1504    1277     255
 
PsiI TTA'TAA

Cuts at:      0    2780    3036 
   Size:       2780     256
 
PstI C_TGCA'G

Cuts at:      0    1931    2205    3036 
   Size:       1931     274     831

  Fragments arranged by size: 

                1931     831     274
 
PvuII CAG'CTG

Cuts at:      0    1684    3036 
   Size:       1684    1352
 
SacI G_AGCT'C

Cuts at:      0     210    1910    3036 
   Size:        210    1700    1126

  Fragments arranged by size: 

                1700    1126     210
 
SacII CC_GC'GG

Cuts at:      0      16      42    3036 
   Size:         16      26    2994

  Fragments arranged by size: 

                2994      26      16
 
SalI G'TCGA_C

Cuts at:      0    1742    3036 
   Size:       1742    1294
 
ScaI AGT'ACT

Cuts at:      0     121    1439    3036 
   Size:        121    1318    1597

  Fragments arranged by size: 

                1597    1318     121
 
SfcI C'TryA_G

Cuts at:      0     818     937    1524    1808    1927    2201    3036 
   Size:        818     119     587     284     119     274     835

  Fragments arranged by size: 

                 835     818     587     284     274     119     119
 
SgrAI Cr'CCGG_yG

Cuts at:      0     284    3036 
   Size:        284    2752
 
SmlI C'TyrA_G

Cuts at:      0    1486    2223    2972    3036 
   Size:       1486     737     749      64

  Fragments arranged by size: 

                1486     749     737      64
 
SnaBI TAC'GTA

Cuts at:      0    2676    3036 
   Size:       2676     360
 
SphI G_CATG'C

Cuts at:      0    1182    3036 
   Size:       1182    1854
 
StyI C'CwwG_G

Cuts at:      0     332    2028    3036 
   Size:        332    1696    1008

  Fragments arranged by size: 

                1696    1008     332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     241    2865    3036 
   Size:         22     219    2624     171

  Fragments arranged by size: 

                2624     219     171      22
 
TatI w'GTAC_w

Cuts at:      0     119    1437    2936    3036 
   Size:        119    1318    1499     100

  Fragments arranged by size: 

                1499    1318     119     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     365     397    3036 
   Size:        365      32    2639

  Fragments arranged by size: 

                2639     365      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1780    3036 
   Size:       1780    1256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     367     425    1017    2814    3036 
   Size:        367      58     592    1797     222

  Fragments arranged by size: 

                1797     592     367     222      58
 
XhoI C'TCGA_G

Cuts at:      0    2223    3036 
   Size:       2223     813
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI     AvaI     BaeI     BanI    BanII     BbsI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstYI     BtgI     BtsI     DraI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MslI   MspA1I     NdeI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII
     SacI    SacII     SalI     ScaI     SfcI    SgrAI     SmlI    SnaBI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI
     BcgI    BfrBI     BglI     BmtI     BpmI    BsaHI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI
    EcoRI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI     SmaI     SpeI     SrfI     SspI     StuI     SwaI
     XbaI     XmaI     XmnI     ZraI
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