Restriction map for
pFN21AB7332
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiWI BspDI
BspEI BspHI BspMI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRV HaeII Hin1I HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 2481 GGTACC G^GTACC
AccI 679 GTCTAC GT^[AC][GT]AC
AclI 2918 AACGTT AA^CGTT
AflIII 2025 ACATGT A^C[GA][CT]GT
Asp700I 1255 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2481 GGTACC G^GTACC
AviII 2877 TGCGCA TGC^GCA
BamHI 415 GGATCC G^GATCC
BanI 2481 GGTACC G^G[CT][GA]CC
BbsI 1962 GAAGAC GAAGAC(2/6)
BclI 245 TGATCA T^GATCA
BfaI 202 CTAG C^TAG
BpuAI 1962 GAAGAC GAAGAC(2/6)
BsaWI 2630 TCCGGT [AT]^CCGG[AT]
BsmBI 52 GAGACG CGTCTC(1/5)
Bsp1407I 1022 TGTACA T^GTACA
BspLU11I 2025 ACATGT A^CATGT
BsrBI 2210 GAGCGG CCGCTC(-3/-3)
BsrGI 1022 TGTACA T^GTACA
BssHII 119 GCGCGC G^CGCGC
BssSI 970 CTCGTG CACGAG(-5/-1)
BstAPI 1345 GCAGTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 90 CGGCCG C^GGCCG
EclXI 90 CGGCCG C^GGCCG
Eco52I 90 CGGCCG C^GGCCG
EcoRI 1783 GAATTC G^AATTC
EcoT14I 1746 CCTTGG C^C[AT][AT]GG
EcoT22I 2006 ATGCAT ATGCA^T
FauI 1704 GCGGG CCCGC(4/6)
FbaI 245 TGATCA T^GATCA
FseI 2761 GGCCGGCC GGCCGG^CC
FspI 2877 TGCGCA TGC^GCA
HgaI 1408 GACGC GACGC(5/10)
HindIII 901 AAGCTT A^AGCTT
KpnI 2481 GGTACC GGTAC^C
MaeI 202 CTAG C^TAG
MspA1I 313 CAGCTG C[AC]G^C[GT]G
NaeI 2762 GCCGGC GCC^GGC
NgoMIV 2762 GCCGGC G^CCGGC
NsiI 2006 ATGCAT ATGCA^T
Psp1406I 2918 AACGTT AA^CGTT
PstI 2130 CTGCAG CTGCA^G
PvuII 313 CAGCTG CAG^CTG
SmaI 253 CCCGGG CCC^GGG
SphI 2238 GCATGC GCATG^C
SspBI 1022 TGTACA T^GTACA
StyI 1746 CCTTGG C^C[AT][AT]GG
XmaCI 253 CCCGGG C^CCGGG
XmaI 253 CCCGGG C^CCGGG
XmnI 1255 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 19 CGCG CG^CG
120 CGCG
ApaI 135 GGGCCC GGGCC^C
641 GGGCCC
AvaI 253 CCCGGG C^[CT]CG[GA]G
1038 CTCGGG
BglII 963 AGATCT A^GATCT
1488 AGATCT
BseRI 815 CTCCTC GAGGAG(10/8)
809 GAGGAG
BsgI 172 CTGCAC GTGCAG(16/14)
1217 GTGCAG
BsmFI 1706 GGGAC GGGAC(10/14)
82 GTCCC
BsmI 1053 GAATGC GAATGC(1/-1)
2551 GAATGC
BsoBI 253 CCCGGG C^[CT]CG[GA]G
1038 CTCGGG
BsrFI 88 ACCGGC [GA]^CCGG[CT]
2762 GCCGGC
BstUI 19 CGCG CG^CG
120 CGCG
Cfr10I 88 ACCGGC [GA]^CCGG[CT]
2762 GCCGGC
DsaI 180 CCACGG C^C[GA][CT]GG
2039 CCGTGG
HincII 1233 GTCAAC GT[CT]^[GA]AC
1405 GTTGAC
HindII 1233 GTCAAC GT[CT]^[GA]AC
1405 GTTGAC
MslI 1641 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2928 CACCATAGTG
MvnI 19 CGCG CG^CG
120 CGCG
NdeI 2009 CATATG CA^TATG
2987 CATATG
PpuMI 80 GGGTCCC [GA]G^G[AT]CC[CT]
2219 AGGACCT
SmlI 2163 CTCAAG C^T[CT][GA]AG
2800 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII