(Circular) (Six-base) MAPSORT of: pFN21AB7332.seq Check: 3343 from: 1 to: 7801 pFN21AB7332 7801 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5611 278 Size: 53 83 186 936 4075 2468 Fragments arranged by size: 4075 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2685 5144 1937 Size: 748 2459 4594 Fragments arranged by size: 4594 2459 748 Acc65I G'GTAC_C Cuts at: 4486 5122 4486 Size: 636 7165 AclI AA'CG_TT Cuts at: 1563 4924 5928 6301 1563 Size: 3361 1004 373 3063 Fragments arranged by size: 3361 3063 1004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 3251 3785 3797 4782 5864 7248 7516 Size: 597 1022 534 12 985 1082 1384 268 Cuts at: 7516 1632 Size: 1917 Fragments arranged by size: 1917 1384 1082 1022 985 597 534 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7604 AflIII A'CryG_T Cuts at: 1203 4030 6700 7677 1203 Size: 2827 2670 977 1327 Fragments arranged by size: 2827 2670 1327 977 AgeI A'CCGG_T Cuts at: 6632 6632 Size: 7801 AhdI GACnn_n'nnGTC Cuts at: 6530 6530 Size: 7801 AleI CACnn'nnGTG Cuts at: 1166 4937 1166 Size: 3771 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4623 1184 Size: 3439 4362 AlwNI CAG_nnn'CTG Cuts at: 5544 7116 5544 Size: 1572 6229 ApaI G_GGCC'C Cuts at: 2144 2650 2144 Size: 506 7295 ApaLI G'TGCA_C Cuts at: 5858 7014 5858 Size: 1156 6645 ApoI r'AATT_y Cuts at: 1493 2573 2584 2741 3452 3788 4492 4699 5110 Size: 1080 11 157 711 336 704 207 411 Cuts at: 5110 5336 5488 7597 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 711 704 411 336 226 207 157 152 11 AseI AT'TA_AT Cuts at: 160 1032 6355 160 Size: 872 5323 1606 Fragments arranged by size: 5323 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 AvaI C'yCGr_G Cuts at: 1870 1943 2258 3043 5126 1870 Size: 73 315 785 2083 4545 Fragments arranged by size: 4545 2083 785 315 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4216 4249 4216 Size: 33 7768 BamHI G'GATC_C Cuts at: 2420 5131 5498 2420 Size: 2711 367 4723 Fragments arranged by size: 4723 2711 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4486 5122 6577 618 Size: 325 217 627 2699 636 1455 1842 Fragments arranged by size: 2699 1842 1455 636 627 325 217 BanII G_rGCy'C Cuts at: 1391 2144 2650 2821 3175 4205 5120 1391 Size: 753 506 171 354 1030 915 4072 Fragments arranged by size: 4072 1030 915 753 506 354 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3974 928 Size: 971 2075 4755 Fragments arranged by size: 4755 2075 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5992 6026 1903 Size: 34 4055 34 3678 Fragments arranged by size: 4055 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5693 6903 5693 Size: 1210 6591 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 7099 BfrBI ATG'CAT Cuts at: 4013 4013 Size: 7801 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6412 136 Size: 107 122 71 631 605 4740 1525 Fragments arranged by size: 4740 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2968 3493 7796 2968 Size: 525 4303 2973 Fragments arranged by size: 4303 2973 525 BlpI GC'TnA_GC Cuts at: 5218 5218 Size: 7801 Bme1580I G_kGCm'C Cuts at: 1790 2144 2511 2650 3874 5862 7018 1790 Size: 354 367 139 1224 1988 1156 2573 Fragments arranged by size: 2573 1988 1224 1156 367 354 139 BmgBI CAC'GTC Cuts at: 1318 2304 3631 1318 Size: 986 1327 5488 Fragments arranged by size: 5488 1327 986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5532 6490 442 Size: 416 53 83 399 24 4115 958 1753 Fragments arranged by size: 4115 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7801 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 4023 4572 6461 1239 Size: 1061 1723 549 1889 2579 Fragments arranged by size: 2579 1889 1723 1061 549 Bpu10I CC'TnA_GC Cuts at: 3386 3386 Size: 7801 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4153 4790 5926 6791 7089 4153 Size: 637 1136 865 298 4865 Fragments arranged by size: 4865 1136 865 637 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6464 882 Size: 617 4965 2219 Fragments arranged by size: 4965 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5592 493 Size: 711 4388 2702 Fragments arranged by size: 4388 2702 711 BsaBI GATnn'nnATC Cuts at: 7795 7795 Size: 7801 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5608 5990 6625 275 Size: 53 83 186 936 4075 382 635 1451 Fragments arranged by size: 4075 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4635 6234 6632 6906 7053 7464 4635 Size: 1599 398 274 147 411 4972 Fragments arranged by size: 4972 1599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2916 2946 1178 Size: 30 165 30 1513 30 6033 Fragments arranged by size: 6033 1513 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2811 2829 1186 Size: 1625 18 6158 Fragments arranged by size: 6158 1625 18 BseYI C'CCAG_C Cuts at: 2320 7004 7500 2320 Size: 4684 496 2621 Fragments arranged by size: 4684 2621 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 3243 1163 Size: 573 426 1081 5721 Fragments arranged by size: 5721 1081 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2098 2172 5251 6012 7040 Size: 275 186 93 4 74 3079 761 1028 Cuts at: 7040 1540 Size: 2301 Fragments arranged by size: 3079 2301 1028 761 275 186 93 74 4 BsiHKAI G_wGCw'C Cuts at: 2821 2986 2999 3175 4205 5862 5947 7018 2821 Size: 165 13 176 1030 1657 85 1071 3604 Fragments arranged by size: 3604 1657 1071 1030 176 165 85 13 BsmI GAATG_Cn' Cuts at: 3064 4562 5312 5405 3064 Size: 1498 750 93 5460 Fragments arranged by size: 5460 1498 750 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2051 2051 Size: 7801 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2511 2650 2821 2986 2999 3175 Size: 399 354 367 139 171 165 13 176 Cuts at: 3175 3874 4205 5120 5862 5947 7018 1391 Size: 699 331 915 742 85 1071 2174 Fragments arranged by size: 2174 1071 915 742 699 399 367 354 331 176 171 165 139 85 13 BspEI T'CCGG_A Cuts at: 7464 7464 Size: 7801 BspHI T'CATG_A Cuts at: 5690 7420 5690 Size: 1730 6071 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5156 844 Size: 4312 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4217 5688 1394 Size: 429 2394 1471 3507 Fragments arranged by size: 3507 2394 1471 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3069 4617 5098 6296 6470 65 Size: 1183 1821 1548 481 1198 174 1396 Fragments arranged by size: 1821 1548 1396 1198 1183 481 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 4767 6445 6632 1700 Size: 110 283 2674 1678 187 2869 Fragments arranged by size: 2869 2674 1678 283 187 110 BsrGI T'GTAC_A Cuts at: 96 3027 96 Size: 2931 4870 BssHII G'CGCG_C Cuts at: 1925 2124 1925 Size: 199 7602 BssSI C'ACGA_G Cuts at: 2975 5861 6873 2975 Size: 2886 1012 3903 Fragments arranged by size: 3903 2886 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3356 794 Size: 2562 5239 BstBI TT'CG_AA Cuts at: 6605 6605 Size: 7801 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7801 BstYI r'GATC_y Cuts at: 1972 2420 2968 3231 3493 5131 5498 5883 5900 Size: 448 548 263 262 1638 367 385 17 Cuts at: 5900 7352 7438 7450 7796 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1638 1452 548 448 385 367 346 263 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2185 4044 513 Size: 552 939 181 1859 4270 Fragments arranged by size: 4270 1859 939 552 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7062 Fragments arranged by size: 7062 683 56 BtsI GCAGTG_nn' Cuts at: 2220 5317 6111 6131 2220 Size: 3097 794 20 3890 Fragments arranged by size: 3890 3097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7766 DraI TTT'AAA Cuts at: 4314 4698 5020 5105 4314 Size: 384 322 85 7010 Fragments arranged by size: 7010 384 322 85 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6808 809 Size: 507 5492 1802 Fragments arranged by size: 5492 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2296 3217 Size: 54 1425 50 275 18 265 201 921 Cuts at: 3217 4769 5248 6137 7766 8 Size: 1552 479 889 1629 43 Fragments arranged by size: 1629 1552 1425 921 889 479 275 265 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2095 5248 1537 Size: 275 283 3153 4090 Fragments arranged by size: 4090 3153 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 3010 3616 4074 4215 4257 5053 5731 Size: 1005 652 606 458 141 42 796 678 Cuts at: 5731 1353 Size: 3423 Fragments arranged by size: 3423 1005 796 678 652 606 458 141 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6388 6762 6908 387 Size: 1064 138 4799 374 146 1280 Fragments arranged by size: 4799 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2819 3173 4203 2819 Size: 354 1030 6417 Fragments arranged by size: 6417 1030 354 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2300 3251 3785 3797 4023 4572 Size: 393 597 71 951 534 12 226 549 Cuts at: 4572 4782 5864 6461 7248 7516 1239 Size: 210 1082 597 787 268 1524 Fragments arranged by size: 1524 1082 951 787 597 597 549 534 393 268 226 210 71 12 EcoO109I rG'GnC_Cy Cuts at: 2086 2140 2141 2646 2647 4225 2086 Size: 54 1 505 1 1578 5662 Fragments arranged by size: 5662 1578 505 54 1 1 EcoRI G'AATT_C Cuts at: 3788 5110 5488 3788 Size: 1322 378 6101 Fragments arranged by size: 6101 1322 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5029 5029 Size: 7801 FseI GG_CCGG'CC Cuts at: 4771 4771 Size: 7801 FspI TGC'GCA Cuts at: 4884 6307 4884 Size: 1423 6378 HaeII r_GCGC'y Cuts at: 1678 6948 1678 Size: 5270 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 2359 2391 2659 Size: 32 135 32 693 32 62 32 268 Cuts at: 2659 2691 2751 2783 2916 2948 2980 3218 3250 Size: 32 60 32 133 32 32 238 32 Cuts at: 3250 3255 3287 4151 4183 4187 4219 6442 6474 Size: 5 32 864 32 4 32 2223 32 Cuts at: 6474 6516 6548 1373 Size: 42 32 2626 Fragments arranged by size: 2626 2223 864 693 268 238 135 133 62 60 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 5 4 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3412 5145 5391 669 Size: 988 281 1302 172 1733 246 3079 Fragments arranged by size: 3079 1733 1302 988 281 246 172 HindIII A'AGCT_T Cuts at: 748 2906 748 Size: 2158 5643 HpaI GTT'AAC Cuts at: 5391 5391 Size: 7801 KpnI G_GTAC'C Cuts at: 4490 5126 4490 Size: 636 7165 MfeI C'AATT_G Cuts at: 5400 5400 Size: 7801 MluI A'CGCG_T Cuts at: 7677 7677 Size: 7801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2749 2791 3061 4780 5048 6915 7099 1305 Size: 1444 42 270 1719 268 1867 184 2007 Fragments arranged by size: 2007 1867 1719 1444 270 268 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5979 Fragments arranged by size: 5979 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3650 4937 5759 6118 6277 518 Size: 648 2484 1287 822 359 159 2042 Fragments arranged by size: 2484 2042 1287 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2320 5217 5894 7042 7287 1606 Size: 714 2897 677 1148 245 2120 Fragments arranged by size: 2897 2120 1148 714 677 245 NaeI GCC'GGC Cuts at: 4769 4769 Size: 7801 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6310 Fragments arranged by size: 6310 939 552 NdeI CA'TA_TG Cuts at: 387 4015 4993 387 Size: 3628 978 3195 Fragments arranged by size: 3628 3195 978 NgoMIV G'CCGG_C Cuts at: 4767 4767 Size: 7801 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7801 NotI GC'GGCC_GC Cuts at: 5248 5248 Size: 7801 NsiI A_TGCA'T Cuts at: 4015 4015 Size: 7801 NspI r_CATG'y Cuts at: 1132 1229 4034 4247 4889 5159 6704 1132 Size: 97 2805 213 642 270 1545 2229 Fragments arranged by size: 2805 2229 1545 642 270 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7801 PciI A'CATG_T Cuts at: 4030 6700 4030 Size: 2670 5131 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6899 PfoI T'CCnGG_A Cuts at: 2052 4191 2052 Size: 2139 5662 PmeI GTTT'AAAC Cuts at: 5105 5105 Size: 7801 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5870 5902 7383 7415 5870 Size: 32 1481 32 6256 Fragments arranged by size: 6256 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 4225 2086 Size: 2139 5662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7801 PsiI TTA'TAA Cuts at: 3555 5371 3555 Size: 1816 5985 PspOMI G'GGCC_C Cuts at: 2140 2646 2140 Size: 506 7295 PstI C_TGCA'G Cuts at: 830 4139 5153 830 Size: 3309 1014 3478 Fragments arranged by size: 3478 3309 1014 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7801 PvuII CAG'CTG Cuts at: 2320 2320 Size: 7801 SacI G_AGCT'C Cuts at: 2821 3175 4205 2821 Size: 354 1030 6417 Fragments arranged by size: 6417 1030 354 SalI G'TCGA_C Cuts at: 1936 5143 1936 Size: 3207 4594 SanDI GG'GwC_CC Cuts at: 2086 2086 Size: 7801 SbfI CC_TGCA'GG Cuts at: 5153 5153 Size: 7801 ScaI AGT'ACT Cuts at: 6049 7761 6049 Size: 1712 6089 SfcI C'TryA_G Cuts at: 826 1045 3117 3399 3673 4135 4299 5149 6284 Size: 219 2072 282 274 462 164 850 1135 Cuts at: 6284 6965 7156 826 Size: 681 191 1471 Fragments arranged by size: 2072 1471 1135 850 681 462 282 274 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7801 SmaI CCC'GGG Cuts at: 1872 2260 5128 1872 Size: 388 2868 4545 Fragments arranged by size: 4545 2868 388 SmlI C'TyrA_G Cuts at: 820 1017 1943 4168 4805 5905 6806 7068 820 Size: 197 926 2225 637 1100 901 262 1553 Fragments arranged by size: 2225 1553 1100 926 901 637 262 197 SnaBI TAC'GTA Cuts at: 493 5592 493 Size: 5099 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7801 SphI G_CATG'C Cuts at: 1132 4247 5159 1132 Size: 3115 912 3774 Fragments arranged by size: 3774 3115 912 SspI AAT'ATT Cuts at: 5 52 5725 5 Size: 47 5673 2081 Fragments arranged by size: 5673 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7801 StyI C'CwwG_G Cuts at: 513 1065 2004 3751 5240 513 Size: 552 939 1747 1489 3074 Fragments arranged by size: 3074 1747 1489 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 2201 3001 3354 5828 5845 5998 Size: 1255 578 43 800 353 2474 17 153 Cuts at: 5998 6183 6628 325 Size: 185 445 1498 Fragments arranged by size: 2474 1498 1255 800 578 445 353 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3027 3779 4668 Size: 274 80 33 51 1443 1050 752 889 Cuts at: 4668 4975 6047 7759 96 Size: 307 1072 1712 138 Fragments arranged by size: 1712 1443 1072 1050 889 752 307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 7761 7793 2412 Size: 32 5317 32 2420 Fragments arranged by size: 5317 2420 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7801 XbaI T'CTAG_A Cuts at: 5137 5137 Size: 7801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4471 4547 4783 4471 Size: 76 236 7489 Fragments arranged by size: 7489 236 76 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7801 XmaI C'CCGG_G Cuts at: 1870 2258 5126 1870 Size: 388 2868 4545 Fragments arranged by size: 4545 2868 388 XmnI GAAnn'nnTTC Cuts at: 3264 5930 3264 Size: 2666 5135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5609 276 Size: 53 83 186 936 4075 2468 Fragments arranged by size: 4075 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI