Restriction map for
pFN21AB7332
(Linear) (Six-base) MAPSORT of: KIBB7332.seq Check: 3480 from: 1 to: 3096
KIBB7332.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 680 3096
Size: 680 2416
Acc65I G'GTAC_C
Cuts at: 0 2481 3096
Size: 2481 615
AclI AA'CG_TT
Cuts at: 0 2919 3096
Size: 2919 177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1246 1780 1792 2777 3096
Size: 224 1022 534 12 985 319
Fragments arranged by size:
1022 985 534 319 224 12
AflIII A'CryG_T
Cuts at: 0 2025 3096
Size: 2025 1071
AleI CACnn'nnGTG
Cuts at: 0 2932 3096
Size: 2932 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2618 3096
Size: 2618 478
ApaI G_GGCC'C
Cuts at: 0 139 645 3096
Size: 139 506 2451
Fragments arranged by size:
2451 506 139
ApoI r'AATT_y
Cuts at: 0 568 579 736 1447 1783 2487 2694 3096
Size: 568 11 157 711 336 704 207 402
Fragments arranged by size:
711 704 568 402 336 207 157 11
AvaI C'yCGr_G
Cuts at: 0 253 1038 3096
Size: 253 785 2058
Fragments arranged by size:
2058 785 253
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2211 2244 3096
Size: 2211 33 852
Fragments arranged by size:
2211 852 33
BamHI G'GATC_C
Cuts at: 0 415 3096
Size: 415 2681
BanI G'GyrC_C
Cuts at: 0 2481 3096
Size: 2481 615
BanII G_rGCy'C
Cuts at: 0 139 645 816 1170 2200 3096
Size: 139 506 171 354 1030 896
Fragments arranged by size:
1030 896 506 354 171 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 1969 3096
Size: 1969 1127
BclI T'GATC_A
Cuts at: 0 245 3096
Size: 245 2851
BfrBI ATG'CAT
Cuts at: 0 2008 3096
Size: 2008 1088
BglII A'GATC_T
Cuts at: 0 963 1488 3096
Size: 963 525 1608
Fragments arranged by size:
1608 963 525
Bme1580I G_kGCm'C
Cuts at: 0 139 506 645 1869 3096
Size: 139 367 139 1224 1227
Fragments arranged by size:
1227 1224 367 139 139
BmgBI CAC'GTC
Cuts at: 0 299 1626 3096
Size: 299 1327 1470
Fragments arranged by size:
1470 1327 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 2018 2567 3096
Size: 295 1723 549 529
Fragments arranged by size:
1723 549 529 295
Bpu10I CC'TnA_GC
Cuts at: 0 1381 3096
Size: 1381 1715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2148 2785 3096
Size: 2148 637 311
Fragments arranged by size:
2148 637 311
BsaWI w'CCGG_w
Cuts at: 0 2630 3096
Size: 2630 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 911 941 3096
Size: 911 30 2155
Fragments arranged by size:
2155 911 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 806 824 3096
Size: 806 18 2272
Fragments arranged by size:
2272 806 18
BseYI C'CCAG_C
Cuts at: 0 315 3096
Size: 315 2781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1238 3096
Size: 157 1081 1858
Fragments arranged by size:
1858 1081 157
BsiEI CG_ry'CG
Cuts at: 0 89 93 167 3096
Size: 89 4 74 2929
Fragments arranged by size:
2929 89 74 4
BsiHKAI G_wGCw'C
Cuts at: 0 816 981 994 1170 2200 3096
Size: 816 165 13 176 1030 896
Fragments arranged by size:
1030 896 816 176 165 13
BsmI GAATG_Cn'
Cuts at: 0 1059 2557 3096
Size: 1059 1498 539
Fragments arranged by size:
1498 1059 539
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 3096
Size: 46 3050
Bsp1286I G_dGCh'C
Cuts at: 0 139 506 645 816 981 994 1170 1869
Size: 139 367 139 171 165 13 176 699
Cuts at: 1869 2200 3096
Size: 331 896
Fragments arranged by size:
896 699 367 331 176 171 165 139
139 13
BsrBI CCG'CTC
Cuts at: 0 2212 3096
Size: 2212 884
BsrDI GCAATG_nn'
Cuts at: 0 1064 2612 3093 3096
Size: 1064 1548 481 3
Fragments arranged by size:
1548 1064 481 3
BsrFI r'CCGG_y
Cuts at: 0 88 2762 3096
Size: 88 2674 334
Fragments arranged by size:
2674 334 88
BsrGI T'GTAC_A
Cuts at: 0 1022 3096
Size: 1022 2074
BssHII G'CGCG_C
Cuts at: 0 119 3096
Size: 119 2977
BssSI C'ACGA_G
Cuts at: 0 970 3096
Size: 970 2126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1351 3096
Size: 1351 1745
BstYI r'GATC_y
Cuts at: 0 415 963 1226 1488 3096
Size: 415 548 263 262 1608
Fragments arranged by size:
1608 548 415 263 262
BtgI C'CryG_G
Cuts at: 0 180 2039 3096
Size: 180 1859 1057
Fragments arranged by size:
1859 1057 180
BtsI GCAGTG_nn'
Cuts at: 0 215 3096
Size: 215 2881
DraI TTT'AAA
Cuts at: 0 2309 2693 3015 3096
Size: 2309 384 322 81
Fragments arranged by size:
2309 384 322 81
EaeI y'GGCC_r
Cuts at: 0 90 291 1212 2764 3096
Size: 90 201 921 1552 332
Fragments arranged by size:
1552 921 332 201 90
EagI C'GGCC_G
Cuts at: 0 90 3096
Size: 90 3006
EarI CTCTTCn'nnn_
Cuts at: 0 353 1005 1611 2069 2210 2252 3048 3096
Size: 353 652 606 458 141 42 796 48
Fragments arranged by size:
796 652 606 458 353 141 48 42
EcoICRI GAG'CTC
Cuts at: 0 814 1168 2198 3096
Size: 814 354 1030 898
Fragments arranged by size:
1030 898 814 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 295 1246 1780 1792 2018 2567 2777
Size: 224 71 951 534 12 226 549 210
Cuts at: 2777 3096
Size: 319
Fragments arranged by size:
951 549 534 319 226 224 210 71
12
EcoO109I rG'GnC_Cy
Cuts at: 0 81 135 136 641 642 2220 3096
Size: 81 54 1 505 1 1578 876
Fragments arranged by size:
1578 876 505 81 54 1 1
EcoRI G'AATT_C
Cuts at: 0 1783 3096
Size: 1783 1313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3024 3096
Size: 3024 72
FseI GG_CCGG'CC
Cuts at: 0 2766 3096
Size: 2766 330
FspI TGC'GCA
Cuts at: 0 2879 3096
Size: 2879 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 354 386 654 686 746 778
Size: 260 32 62 32 268 32 60 32
Cuts at: 778 911 943 975 1213 1245 1250 1282 2146
Size: 133 32 32 238 32 5 32 864
Cuts at: 2146 2178 2182 2214 3096
Size: 32 4 32 882
Fragments arranged by size:
882 864 268 260 238 133 62 60
32 32 32 32 32 32 32 32
32 32 5 4
HincII GTy'rAC
Cuts at: 0 1235 1407 3096
Size: 1235 172 1689
Fragments arranged by size:
1689 1235 172
HindIII A'AGCT_T
Cuts at: 0 901 3096
Size: 901 2195
KpnI G_GTAC'C
Cuts at: 0 2485 3096
Size: 2485 611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 786 1056 2775 3043 3096
Size: 744 42 270 1719 268 53
Fragments arranged by size:
1719 744 270 268 53 42
MslI CAynn'nnrTG
Cuts at: 0 1645 2932 3096
Size: 1645 1287 164
Fragments arranged by size:
1645 1287 164
MspA1I CmG'CkG
Cuts at: 0 315 3096
Size: 315 2781
NaeI GCC'GGC
Cuts at: 0 2764 3096
Size: 2764 332
NdeI CA'TA_TG
Cuts at: 0 2010 2988 3096
Size: 2010 978 108
Fragments arranged by size:
2010 978 108
NgoMIV G'CCGG_C
Cuts at: 0 2762 3096
Size: 2762 334
NsiI A_TGCA'T
Cuts at: 0 2010 3096
Size: 2010 1086
NspI r_CATG'y
Cuts at: 0 2029 2242 2884 3096
Size: 2029 213 642 212
Fragments arranged by size:
2029 642 213 212
PciI A'CATG_T
Cuts at: 0 2025 3096
Size: 2025 1071
PfoI T'CCnGG_A
Cuts at: 0 47 2186 3096
Size: 47 2139 910
Fragments arranged by size:
2139 910 47
PpuMI rG'GwC_Cy
Cuts at: 0 81 2220 3096
Size: 81 2139 876
Fragments arranged by size:
2139 876 81
PsiI TTA'TAA
Cuts at: 0 1550 3096
Size: 1550 1546
PspOMI G'GGCC_C
Cuts at: 0 135 641 3096
Size: 135 506 2455
Fragments arranged by size:
2455 506 135
PstI C_TGCA'G
Cuts at: 0 2134 3096
Size: 2134 962
PvuII CAG'CTG
Cuts at: 0 315 3096
Size: 315 2781
SacI G_AGCT'C
Cuts at: 0 816 1170 2200 3096
Size: 816 354 1030 896
Fragments arranged by size:
1030 896 816 354
SanDI GG'GwC_CC
Cuts at: 0 81 3096
Size: 81 3015
SfcI C'TryA_G
Cuts at: 0 1112 1394 1668 2130 2294 3096
Size: 1112 282 274 462 164 802
Fragments arranged by size:
1112 802 462 282 274 164
SmaI CCC'GGG
Cuts at: 0 255 3096
Size: 255 2841
SmlI C'TyrA_G
Cuts at: 0 2163 2800 3096
Size: 2163 637 296
Fragments arranged by size:
2163 637 296
SphI G_CATG'C
Cuts at: 0 2242 3096
Size: 2242 854
StyI C'CwwG_G
Cuts at: 0 1746 3096
Size: 1746 1350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 196 996 1349 3096
Size: 153 43 800 353 1747
Fragments arranged by size:
1747 800 353 153 43
TatI w'GTAC_w
Cuts at: 0 1022 1774 2663 2970 3096
Size: 1022 752 889 307 126
Fragments arranged by size:
1022 889 752 307 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 3096
Size: 407 32 2657
Fragments arranged by size:
2657 407 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2466 2542 2778 3096
Size: 2466 76 236 318
Fragments arranged by size:
2466 318 236 76
XmaI C'CCGG_G
Cuts at: 0 253 3096
Size: 253 2843
XmnI GAAnn'nnTTC
Cuts at: 0 1259 3096
Size: 1259 1837
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbsI BclI
BfrBI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstYI BtgI BtsI DraI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI Hin4I HincII
HindIII KpnI MmeI MslI MspA1I NaeI NdeI NgoMIV
NsiI NspI PciI PfoI PpuMI PsiI PspOMI PstI
PvuII SacI SanDI SfcI SmaI SmlI SphI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BcgI
BciVI BglI BlpI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsiWI BspEI BspHI BspMI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EciI EcoNI EcoRV FspAI HaeII HpaI KasI MfeI
MluI MscI NarI NcoI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XhoI ZraI