(Circular) (Six-base) MAPSORT of: pFN21AB9729.seq Check: 7086 from: 1 to: 6043 pFN21AB9729 6043 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3329 3329 Size: 6043 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2167 3386 1937 Size: 230 1219 4594 Fragments arranged by size: 4594 1219 230 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 4106 5490 5758 1632 Size: 1200 1274 1384 268 1917 Fragments arranged by size: 1917 1384 1274 1200 268 AfeI AGC'GCT Cuts at: 3088 3088 Size: 6043 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 3182 4772 3182 Size: 1590 4453 AleI CACnn'nnGTG Cuts at: 1166 2579 1166 Size: 1413 4630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2053 2878 3786 5358 2053 Size: 825 908 1572 2738 Fragments arranged by size: 2738 1572 908 825 ApaLI G'TGCA_C Cuts at: 3099 4100 5256 3099 Size: 1001 1156 3886 Fragments arranged by size: 3886 1156 1001 ApoI r'AATT_y Cuts at: 1493 2321 3352 3578 3730 5839 1493 Size: 828 1031 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2466 2748 2901 3368 1870 Size: 73 523 282 153 467 4545 Fragments arranged by size: 4545 523 467 282 153 73 AvrII C'CTAG_G Cuts at: 3014 3116 3014 Size: 102 5941 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2015 2048 2015 Size: 33 6010 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 2441 2610 2641 3364 Size: 325 217 627 411 243 169 31 723 Cuts at: 3364 4819 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 723 627 411 325 243 217 169 31 BanII G_rGCy'C Cuts at: 1391 2286 2757 3362 1391 Size: 895 471 605 4072 Fragments arranged by size: 4072 895 605 471 BbeI G_GCGC'C Cuts at: 2202 2445 2614 2645 2202 Size: 243 169 31 5600 Fragments arranged by size: 5600 243 169 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2781 2820 3204 928 Size: 971 882 39 384 3767 Fragments arranged by size: 3767 971 882 384 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2305 3103 3287 4104 5260 1790 Size: 515 798 184 817 1156 2573 Fragments arranged by size: 2573 1156 817 798 515 184 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 3774 4732 Size: 416 53 83 399 24 624 1733 958 Cuts at: 4732 442 Size: 1753 Fragments arranged by size: 1753 1733 958 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2739 1056 Size: 1683 4360 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2305 2710 2305 Size: 405 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2801 3348 4703 1239 Size: 1562 547 1355 2579 Fragments arranged by size: 2579 1562 1355 547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 2600 4168 5033 5331 2471 Size: 129 1568 865 298 3183 Fragments arranged by size: 3183 1568 865 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2465 3066 4706 882 Size: 617 966 601 1640 2219 Fragments arranged by size: 2219 1640 966 617 601 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2199 2442 2611 Size: 53 83 186 936 524 142 243 169 Cuts at: 2611 2642 3850 4232 4867 275 Size: 31 1208 382 635 1451 Fragments arranged by size: 1451 1208 936 635 524 382 243 186 169 142 83 53 31 BsaWI w'CCGG_w Cuts at: 2188 2969 4476 4874 5148 5295 5706 2188 Size: 781 1507 398 274 147 411 2525 Fragments arranged by size: 2525 1507 781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 2697 2702 2804 2807 2810 2813 2816 Size: 1492 19 5 102 3 3 3 3 Cuts at: 2816 2834 3307 1186 Size: 18 473 3922 Fragments arranged by size: 3922 1492 473 102 19 18 5 3 3 3 3 BseYI C'CCAG_C Cuts at: 2131 2173 2194 2837 3106 5246 5742 2131 Size: 42 21 643 269 2140 496 2432 Fragments arranged by size: 2432 2140 643 496 269 42 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3329 1163 Size: 573 1593 3877 Fragments arranged by size: 3877 1593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2189 2235 2449 3493 4254 5282 Size: 275 186 188 46 214 1044 761 1028 Cuts at: 5282 1540 Size: 2301 Fragments arranged by size: 2301 1044 1028 761 275 214 188 186 46 BsiHKAI G_wGCw'C Cuts at: 2224 2508 3103 4104 4189 5260 2224 Size: 284 595 1001 85 1071 3007 Fragments arranged by size: 3007 1071 1001 595 284 85 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2224 2286 2305 2508 2757 3103 3287 Size: 399 434 62 19 203 249 346 184 Cuts at: 3287 3362 4104 4189 5260 1391 Size: 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 434 399 346 249 203 184 85 75 62 19 BspEI T'CCGG_A Cuts at: 2969 5706 2969 Size: 2737 3306 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2274 3329 3398 844 Size: 1430 1055 69 3489 Fragments arranged by size: 3489 1430 1055 69 BsrBI CCG'CTC Cuts at: 1394 1823 2141 2389 3930 1394 Size: 429 318 248 1541 3507 Fragments arranged by size: 3507 1541 429 318 248 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2444 2607 4687 4874 1700 Size: 110 634 163 2080 187 2869 Fragments arranged by size: 2869 2080 634 187 163 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 2868 1925 Size: 943 5100 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 2228 2878 2928 794 Size: 1285 149 650 50 3909 Fragments arranged by size: 3909 1285 650 149 50 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstYI r'GATC_y Cuts at: 1972 3373 3740 4125 4142 5594 5680 5692 6038 Size: 1401 367 385 17 1452 86 12 346 Cuts at: 6038 1972 Size: 1977 Fragments arranged by size: 1977 1452 1401 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2035 3287 513 Size: 552 970 1252 3269 Fragments arranged by size: 3269 1252 970 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 2185 2433 2876 3317 3559 4353 4373 2185 Size: 248 443 441 242 794 20 3855 Fragments arranged by size: 3855 794 443 441 248 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2550 2829 Size: 54 1425 50 275 18 402 318 279 Cuts at: 2829 3490 4379 6008 8 Size: 661 889 1629 43 Fragments arranged by size: 1629 1425 889 661 402 318 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 3490 1537 Size: 275 420 1258 4090 Fragments arranged by size: 4090 1258 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2781 3973 1353 Size: 1428 1192 3423 Fragments arranged by size: 3423 1428 1192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3014 4630 5004 5150 387 Size: 1064 138 1425 1616 374 146 1280 Fragments arranged by size: 1616 1425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2801 2832 3348 4106 4703 5490 5758 Size: 393 1169 31 516 758 597 787 268 Cuts at: 5758 1239 Size: 1524 Fragments arranged by size: 1524 1169 787 758 597 516 393 268 31 EcoNI CCTnn'n_nnAGG Cuts at: 2576 2576 Size: 6043 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FspI TGC'GCA Cuts at: 2152 4549 2152 Size: 2397 3646 HaeII r_GCGC'y Cuts at: 1678 2043 2138 2202 2445 2614 2645 3090 5190 Size: 365 95 64 243 169 31 445 2100 Cuts at: 5190 1678 Size: 2531 Fragments arranged by size: 2531 2100 445 365 243 169 95 64 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2978 3010 3168 Size: 32 135 32 530 32 844 32 158 Cuts at: 3168 3200 4684 4716 4758 4790 1373 Size: 32 1484 32 42 32 2626 Fragments arranged by size: 2626 1484 844 530 158 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2168 3021 3387 3633 669 Size: 988 281 230 853 366 246 3079 Fragments arranged by size: 3079 988 853 366 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 2198 2441 2610 2641 2198 Size: 243 169 31 5600 Fragments arranged by size: 5600 243 169 31 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5157 5341 1305 Size: 3852 184 2007 Fragments arranged by size: 3852 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2552 2831 10 Size: 54 1425 343 720 279 3222 Fragments arranged by size: 3222 1425 720 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 2579 4001 4360 4519 518 Size: 648 1398 15 1422 359 159 2042 Fragments arranged by size: 2042 1422 1398 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2037 2237 2243 2837 3268 3289 3459 4136 Size: 431 200 6 594 431 21 170 677 Cuts at: 4136 5284 5529 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 431 431 245 200 170 21 6 NaeI GCC'GGC Cuts at: 2609 2609 Size: 6043 NarI GG'CG_CC Cuts at: 2199 2442 2611 2642 2199 Size: 243 169 31 5600 Fragments arranged by size: 5600 243 169 31 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5491 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NgoMIV G'CCGG_C Cuts at: 2607 2607 Size: 6043 NheI G'CTAG_C Cuts at: 1052 2735 1052 Size: 1683 4360 NotI GC'GGCC_GC Cuts at: 2232 3490 2232 Size: 1258 4785 NspI r_CATG'y Cuts at: 1132 1229 2082 3401 4946 1132 Size: 97 853 1319 1545 2229 Fragments arranged by size: 2229 1545 1319 853 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2021 2053 1108 Size: 913 32 5098 Fragments arranged by size: 5098 913 32 PfoI T'CCnGG_A Cuts at: 3148 3148 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 2242 2338 2899 3258 3395 830 Size: 1412 96 561 359 137 3478 Fragments arranged by size: 3478 1412 561 359 137 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2243 2837 2243 Size: 594 5449 SacII CC_GC'GG Cuts at: 2038 3290 2038 Size: 1252 4791 SalI G'TCGA_C Cuts at: 1936 2166 3385 1936 Size: 230 1219 4594 Fragments arranged by size: 4594 1219 230 SbfI CC_TGCA'GG Cuts at: 3258 3395 3258 Size: 137 5906 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SexAI A'CCwGG_T Cuts at: 2588 2588 Size: 6043 SfcI C'TryA_G Cuts at: 826 1045 2238 2272 2334 2895 3254 3303 3391 Size: 219 1193 34 62 561 359 49 88 Cuts at: 3391 4526 5207 5398 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1193 1135 681 561 359 219 191 88 62 49 34 SfoI GGC'GCC Cuts at: 2200 2443 2612 2643 2200 Size: 243 169 31 5600 Fragments arranged by size: 5600 243 169 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 2607 1700 Size: 907 5136 SmaI CCC'GGG Cuts at: 1872 2468 2750 3370 1872 Size: 596 282 620 4545 Fragments arranged by size: 4545 620 596 282 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 2615 2901 4147 5048 5310 Size: 197 926 543 129 286 1246 901 262 Cuts at: 5310 820 Size: 1553 Fragments arranged by size: 1553 1246 926 901 543 286 262 197 129 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2492 1517 Size: 975 5068 StyI C'CwwG_G Cuts at: 513 1065 2205 3014 3116 3482 513 Size: 552 1140 809 102 366 3074 Fragments arranged by size: 3074 1140 809 552 366 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2591 4070 4087 4240 4425 4870 325 Size: 1255 1011 1479 17 153 185 445 1498 Fragments arranged by size: 1498 1479 1255 1011 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2480 4289 6001 Size: 274 80 33 51 1443 503 1809 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1809 1712 1443 503 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6003 Size: 32 6011 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2255 3323 2255 Size: 1068 4975 XhoI C'TCGA_G Cuts at: 1943 2901 1943 Size: 958 5085 XmaI C'CCGG_G Cuts at: 1870 2466 2748 3368 1870 Size: 596 282 620 4545 Fragments arranged by size: 4545 620 596 282 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI