(Circular) (Six-base) MAPSORT of: pFN21AE0300.seq Check: 5224 from: 1 to: 9214 pFN21AE0300 9214 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2128 5563 6000 6198 2128 Size: 3435 437 198 5144 Fragments arranged by size: 5144 3435 437 198 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7024 278 Size: 53 83 186 936 5488 2468 Fragments arranged by size: 5488 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3264 6096 6557 1937 Size: 1327 2832 461 4594 Fragments arranged by size: 4594 2832 1327 461 Acc65I G'GTAC_C Cuts at: 4447 4489 6535 4447 Size: 42 2046 7126 Fragments arranged by size: 7126 2046 42 AclI AA'CG_TT Cuts at: 1563 7341 7714 1563 Size: 5778 373 3063 Fragments arranged by size: 5778 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 2582 2690 3225 3311 4223 4290 4301 Size: 932 18 108 535 86 912 67 11 Cuts at: 4301 7277 8661 8929 1632 Size: 2976 1384 268 1917 Fragments arranged by size: 2976 1917 1384 932 912 535 268 108 86 67 18 11 AfeI AGC'GCT Cuts at: 3714 5028 3714 Size: 1314 7900 AflII C'TTAA_G Cuts at: 820 1017 4250 820 Size: 197 3233 5784 Fragments arranged by size: 5784 3233 197 AflIII A'CryG_T Cuts at: 1203 3871 4532 8113 9090 1203 Size: 2668 661 3581 977 1327 Fragments arranged by size: 3581 2668 1327 977 661 AgeI A'CCGG_T Cuts at: 8045 8045 Size: 9214 AhdI GACnn_n'nnGTC Cuts at: 4951 7943 4951 Size: 2992 6222 AleI CACnn'nnGTG Cuts at: 1166 5261 1166 Size: 4095 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2118 4439 5457 1184 Size: 934 2321 1018 4941 Fragments arranged by size: 4941 2321 1018 934 AlwNI CAG_nnn'CTG Cuts at: 3418 3554 4589 4973 5607 6957 8529 3418 Size: 136 1035 384 634 1350 1572 4103 Fragments arranged by size: 4103 1572 1350 1035 634 384 136 ApaI G_GGCC'C Cuts at: 3561 5765 6138 3561 Size: 2204 373 6637 Fragments arranged by size: 6637 2204 373 ApaLI G'TGCA_C Cuts at: 3212 7271 8427 3212 Size: 4059 1156 3999 Fragments arranged by size: 4059 3999 1156 ApoI r'AATT_y Cuts at: 1493 2237 4608 6523 6749 6901 9010 1493 Size: 744 2371 1915 226 152 2109 1697 Fragments arranged by size: 2371 2109 1915 1697 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 2177 7768 160 Size: 872 1145 5591 1606 Fragments arranged by size: 5591 1606 1145 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9214 AvaI C'yCGr_G Cuts at: 1870 1943 3887 5123 5147 5342 5365 5414 5429 Size: 73 1944 1236 24 195 23 49 15 Cuts at: 5429 5810 6041 6539 1870 Size: 381 231 498 4545 Fragments arranged by size: 4545 1944 1236 498 381 231 195 73 49 24 23 15 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4374 4407 4374 Size: 33 9181 BamHI G'GATC_C Cuts at: 6544 6911 6544 Size: 367 8847 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 4447 4489 5153 5988 Size: 325 217 627 326 2334 42 664 835 Cuts at: 5988 6400 6535 7990 618 Size: 412 135 1455 1842 Fragments arranged by size: 2334 1842 1455 835 664 627 412 326 325 217 135 42 BanII G_rGCy'C Cuts at: 1391 3561 3896 5343 5765 6138 6533 1391 Size: 2170 335 1447 422 373 395 4072 Fragments arranged by size: 4072 2170 1447 422 395 373 335 BbeI G_GCGC'C Cuts at: 2117 6404 2117 Size: 4287 4927 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 2311 4422 4806 5595 6504 928 Size: 971 393 19 2111 384 789 909 3638 Fragments arranged by size: 3638 2111 971 909 789 393 384 19 BbvCI CC'TCA_GC Cuts at: 5921 5969 5921 Size: 48 9166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3335 3369 5693 5727 7405 Size: 34 50 34 1314 34 2324 34 1678 Cuts at: 7405 7439 1903 Size: 34 3678 Fragments arranged by size: 3678 2324 1678 1314 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2212 7106 8316 2212 Size: 4894 1210 3110 Fragments arranged by size: 4894 3110 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9214 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7825 136 Size: 107 122 71 631 605 6153 1525 Fragments arranged by size: 6153 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 9209 9209 Size: 9214 BlpI GC'TnA_GC Cuts at: 3447 6314 6631 3447 Size: 2867 317 6030 Fragments arranged by size: 6030 2867 317 Bme1580I G_kGCm'C Cuts at: 1790 2483 3216 3561 5073 5156 5765 5961 6138 Size: 693 733 345 1512 83 609 196 177 Cuts at: 6138 7275 8431 1790 Size: 1137 1156 2573 Fragments arranged by size: 2573 1512 1156 1137 733 693 609 345 196 177 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4775 6945 Size: 416 53 83 399 24 2519 839 2170 Cuts at: 6945 7903 442 Size: 958 1753 Fragments arranged by size: 2519 2170 1753 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2919 3149 3627 4961 5024 5500 5629 7874 Size: 1680 230 478 1334 63 476 129 2245 Cuts at: 7874 1239 Size: 2579 Fragments arranged by size: 2579 2245 1680 1334 478 476 230 129 63 Bpu10I CC'TnA_GC Cuts at: 3419 3779 5921 5969 6167 6203 3419 Size: 360 2142 48 198 36 6430 Fragments arranged by size: 6430 2142 360 198 48 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2379 3815 7339 8204 8502 2379 Size: 1436 3524 865 298 3091 Fragments arranged by size: 3524 3091 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3009 3187 4673 5936 6107 7877 882 Size: 617 1510 178 1486 1263 171 1770 2219 Fragments arranged by size: 2219 1770 1510 1486 1263 617 178 171 BsaAI yAC'GTr Cuts at: 493 1204 3874 4533 7005 493 Size: 711 2670 659 2472 2702 Fragments arranged by size: 2702 2670 2472 711 659 BsaBI GATnn'nnATC Cuts at: 9208 9208 Size: 9214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2114 6401 7021 7403 Size: 53 83 186 936 581 4287 620 382 Cuts at: 7403 8038 275 Size: 635 1451 Fragments arranged by size: 4287 1451 936 635 620 581 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3520 7647 8045 8319 8466 8877 3520 Size: 4127 398 274 147 411 3857 Fragments arranged by size: 4127 3857 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8989 Fragments arranged by size: 8989 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3307 3812 4691 4900 5372 5876 6159 1186 Size: 2121 505 879 209 472 504 283 4241 Fragments arranged by size: 4241 2121 879 505 504 472 283 209 BseYI C'CCAG_C Cuts at: 2075 2308 2350 3123 3367 3765 4524 4966 5568 Size: 233 42 773 244 398 759 442 602 Cuts at: 5568 6130 6175 6181 8417 8913 2075 Size: 562 45 6 2236 496 2376 Fragments arranged by size: 2376 2236 773 759 602 562 496 442 398 244 233 45 42 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2182 2765 2774 4463 5656 1163 Size: 573 388 58 583 9 1689 1193 4721 Fragments arranged by size: 4721 1689 1193 583 573 388 58 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5665 6664 7425 8453 1540 Size: 275 186 3664 999 761 1028 2301 Fragments arranged by size: 3664 2301 1028 999 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 3216 4418 5952 6377 7275 7360 8431 2700 Size: 516 1202 1534 425 898 85 1071 3483 Fragments arranged by size: 3483 1534 1202 1071 898 516 425 85 BsmI GAATG_Cn' Cuts at: 3332 4261 5626 6725 6818 3332 Size: 929 1365 1099 93 5728 Fragments arranged by size: 5728 1365 1099 929 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5267 5267 Size: 9214 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2483 2700 3216 3561 3896 4418 5073 Size: 399 693 217 516 345 335 522 655 Cuts at: 5073 5156 5343 5765 5952 5961 6138 6377 6533 Size: 83 187 422 187 9 177 239 156 Cuts at: 6533 7275 7360 8431 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 693 655 522 516 422 399 345 335 239 217 187 187 177 156 85 83 9 BspEI T'CCGG_A Cuts at: 8877 8877 Size: 9214 BspHI T'CATG_A Cuts at: 7103 8833 7103 Size: 1730 7484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2128 2862 3240 4009 4435 5563 5904 5919 Size: 1284 734 378 769 426 1128 341 15 Cuts at: 5919 6000 6198 6318 6569 844 Size: 81 198 120 251 3489 Fragments arranged by size: 3489 1284 1128 769 734 426 378 341 251 198 120 81 15 BsrBI CCG'CTC Cuts at: 1394 1823 5144 6171 7101 1394 Size: 429 3321 1027 930 3507 Fragments arranged by size: 3507 3321 1027 930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 6435 7709 7883 65 Size: 1183 3173 2014 1274 174 1396 Fragments arranged by size: 3173 2014 1396 1274 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2066 3363 4818 5083 5237 6364 Size: 110 207 49 1297 1455 265 154 1127 Cuts at: 6364 7858 8045 1700 Size: 1494 187 2869 Fragments arranged by size: 2869 1494 1455 1297 1127 265 207 187 154 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9214 BssSI C'ACGA_G Cuts at: 3505 7274 8286 3505 Size: 3769 1012 4433 Fragments arranged by size: 4433 3769 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 2788 3220 5956 794 Size: 1985 9 432 2736 4052 Fragments arranged by size: 4052 2736 1985 432 9 BstBI TT'CG_AA Cuts at: 4464 8018 4464 Size: 3554 5660 BstEII G'GTnAC_C Cuts at: 1184 2663 1184 Size: 1479 7735 BstXI CCAn_nnnn'nTGG Cuts at: 5524 6130 5524 Size: 606 8608 BstYI r'GATC_y Cuts at: 1972 3550 5045 6280 6544 6911 7296 7313 8765 Size: 1578 1495 1235 264 367 385 17 1452 Cuts at: 8765 8851 8863 9209 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1578 1495 1452 1235 385 367 346 264 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3596 6187 3596 Size: 2591 6623 BtgI C'CryG_G Cuts at: 513 1065 2754 3198 5178 5583 5685 513 Size: 552 1689 444 1980 405 102 4042 Fragments arranged by size: 4042 1980 1689 552 444 405 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2938 4799 5199 5256 5655 487 Size: 683 56 1712 1861 400 57 399 4046 Fragments arranged by size: 4046 1861 1712 683 400 399 57 56 BtsI GCAGTG_nn' Cuts at: 2077 3284 6035 6730 7524 7544 2077 Size: 1207 2751 695 794 20 3747 Fragments arranged by size: 3747 2751 1207 794 695 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9214 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9179 DraI TTT'AAA Cuts at: 3997 4854 6518 3997 Size: 857 1664 6693 Fragments arranged by size: 6693 1664 857 DraIII CAC_nnn'GTG Cuts at: 5956 5956 Size: 9214 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3260 8221 809 Size: 507 1944 4961 1802 Fragments arranged by size: 4961 1944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 3580 5191 Size: 54 1425 50 275 18 933 817 1611 Cuts at: 5191 6018 6389 6661 7550 9179 8 Size: 827 371 272 889 1629 43 Fragments arranged by size: 1629 1611 1425 933 889 827 817 371 275 272 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6661 1537 Size: 275 4849 4090 Fragments arranged by size: 4849 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 7144 1353 Size: 5791 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 5371 6345 6501 7801 8175 Size: 1064 138 455 3327 974 156 1300 374 Cuts at: 8175 8321 387 Size: 146 1280 Fragments arranged by size: 3327 1300 1280 1064 974 455 374 156 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2582 2690 2919 3149 3225 3311 Size: 393 932 18 108 229 230 76 86 Cuts at: 3311 3627 4223 4290 4301 4961 5024 5500 5629 Size: 316 596 67 11 660 63 476 129 Cuts at: 5629 7277 7874 8661 8929 1239 Size: 1648 597 787 268 1524 Fragments arranged by size: 1648 1524 932 787 660 597 596 476 393 316 268 230 229 129 108 86 76 67 63 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 4940 5837 4940 Size: 897 8317 EcoO109I rG'GnC_Cy Cuts at: 2215 3415 3601 4650 5362 5761 5893 6134 6135 Size: 1200 186 1049 712 399 132 241 1 Cuts at: 6135 2215 Size: 5294 Fragments arranged by size: 5294 1200 1049 712 399 241 186 132 1 EcoRI G'AATT_C Cuts at: 6523 6901 6523 Size: 378 8836 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2800 2476 Size: 324 8890 FspI TGC'GCA Cuts at: 2684 7720 2684 Size: 5036 4178 HaeII r_GCGC'y Cuts at: 1678 2117 3716 3779 5030 6404 8361 1678 Size: 439 1599 63 1251 1374 1957 2531 Fragments arranged by size: 2531 1957 1599 1374 1251 439 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 4634 4666 4840 Size: 32 135 32 707 32 2323 32 174 Cuts at: 4840 4872 4937 4969 5122 5154 5318 5336 5350 Size: 32 65 32 153 32 164 18 14 Cuts at: 5350 5368 6257 6289 7855 7887 7929 7961 1373 Size: 18 889 32 1566 32 42 32 2626 Fragments arranged by size: 2626 2323 1566 889 707 174 164 153 135 65 42 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3265 6558 6804 669 Size: 988 281 1327 3293 246 3079 Fragments arranged by size: 3293 3079 1327 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9214 HpaI GTT'AAC Cuts at: 6804 6804 Size: 9214 KasI G'GCGC_C Cuts at: 2113 6400 2113 Size: 4287 4927 KpnI G_GTAC'C Cuts at: 4451 4493 6539 4451 Size: 42 2046 7126 Fragments arranged by size: 7126 2046 42 MfeI C'AATT_G Cuts at: 6813 6813 Size: 9214 MluI A'CGCG_T Cuts at: 9090 9090 Size: 9214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2587 2853 3090 3345 3625 5913 8328 8512 Size: 1282 266 237 255 280 2288 2415 184 Cuts at: 8512 1305 Size: 2007 Fragments arranged by size: 2415 2288 2007 1282 280 266 255 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7392 Fragments arranged by size: 7392 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4230 5261 7172 7531 7690 518 Size: 648 3064 1031 1911 359 159 2042 Fragments arranged by size: 3064 2042 1911 1031 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3127 3200 3250 3579 5199 5285 5637 Size: 1150 371 73 50 329 1620 86 352 Cuts at: 5637 6017 6630 7307 8455 8700 1606 Size: 380 613 677 1148 245 2120 Fragments arranged by size: 2120 1620 1150 1148 677 613 380 371 352 329 245 86 73 50 NaeI GCC'GGC Cuts at: 2019 2068 4820 5085 5239 2019 Size: 49 2752 265 154 5994 Fragments arranged by size: 5994 2752 265 154 49 NarI GG'CG_CC Cuts at: 2114 6401 2114 Size: 4287 4927 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8662 NdeI CA'TA_TG Cuts at: 387 387 Size: 9214 NgoMIV G'CCGG_C Cuts at: 2017 2066 4818 5083 5237 2017 Size: 49 2752 265 154 5994 Fragments arranged by size: 5994 2752 265 154 49 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9214 NotI GC'GGCC_GC Cuts at: 6661 6661 Size: 9214 NspI r_CATG'y Cuts at: 1132 1229 2704 4345 6572 8117 1132 Size: 97 1475 1641 2227 1545 2229 Fragments arranged by size: 2229 2227 1641 1545 1475 97 PasI CC'CwG_GG Cuts at: 1348 5293 1348 Size: 3945 5269 PciI A'CATG_T Cuts at: 8113 8113 Size: 9214 PflMI CCAn_nnn'nTGG Cuts at: 1108 4639 1108 Size: 3531 5683 PfoI T'CCnGG_A Cuts at: 4551 4758 4938 4551 Size: 207 180 8827 Fragments arranged by size: 8827 207 180 PmeI GTTT'AAAC Cuts at: 6518 6518 Size: 9214 PmlI CAC'GTG Cuts at: 3874 4533 3874 Size: 659 8555 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7283 7315 8796 8828 7283 Size: 32 1481 32 7669 Fragments arranged by size: 7669 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 3601 4650 5362 5893 2215 Size: 1386 1049 712 531 5536 Fragments arranged by size: 5536 1386 1049 712 531 PshAI GACnn'nnGTC Cuts at: 1613 4578 1613 Size: 2965 6249 PsiI TTA'TAA Cuts at: 6784 6784 Size: 9214 PspOMI G'GGCC_C Cuts at: 3557 5761 6134 3557 Size: 2204 373 6637 Fragments arranged by size: 6637 2204 373 PstI C_TGCA'G Cuts at: 830 2128 2733 2977 3243 6566 830 Size: 1298 605 244 266 3323 3478 Fragments arranged by size: 3478 3323 1298 605 266 244 PvuI CG_AT'CG Cuts at: 2001 5665 2001 Size: 3664 5550 PvuII CAG'CTG Cuts at: 3127 5285 3127 Size: 2158 7056 SacII CC_GC'GG Cuts at: 2757 3201 2757 Size: 444 8770 SalI G'TCGA_C Cuts at: 1936 3263 6556 1936 Size: 1327 3293 4594 Fragments arranged by size: 4594 3293 1327 SanDI GG'GwC_CC Cuts at: 3601 3601 Size: 9214 SbfI CC_TGCA'GG Cuts at: 6566 6566 Size: 9214 ScaI AGT'ACT Cuts at: 6347 7462 9174 6347 Size: 1115 1712 6387 Fragments arranged by size: 6387 1712 1115 SfcI C'TryA_G Cuts at: 826 1045 2124 2376 2729 2973 3239 6562 7697 Size: 219 1079 252 353 244 266 3323 1135 Cuts at: 7697 8378 8569 826 Size: 681 191 1471 Fragments arranged by size: 3323 1471 1135 1079 681 353 266 252 244 219 191 SfoI GGC'GCC Cuts at: 2115 6402 2115 Size: 4287 4927 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9214 SgrAI Cr'CCGG_yG Cuts at: 1700 2017 1700 Size: 317 8897 SmaI CCC'GGG Cuts at: 1872 5367 6541 1872 Size: 3495 1174 4545 Fragments arranged by size: 4545 3495 1174 SmlI C'TyrA_G Cuts at: 820 1017 1943 2394 3794 4250 7318 8219 8481 Size: 197 926 451 1400 456 3068 901 262 Cuts at: 8481 820 Size: 1553 Fragments arranged by size: 3068 1553 1400 926 901 456 451 262 197 SnaBI TAC'GTA Cuts at: 493 7005 493 Size: 6512 2702 SpeI A'CTAG_T Cuts at: 152 5065 152 Size: 4913 4301 SphI G_CATG'C Cuts at: 1132 6572 1132 Size: 5440 3774 SspI AAT'ATT Cuts at: 5 52 2175 2341 2593 3957 7138 5 Size: 47 2123 166 252 1364 3181 2081 Fragments arranged by size: 3181 2123 2081 1364 252 166 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9214 StyI C'CwwG_G Cuts at: 513 1065 5385 6653 513 Size: 552 4320 1268 3074 Fragments arranged by size: 4320 3074 1268 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 2999 3416 5960 6479 7241 7258 Size: 1255 1071 348 417 2544 519 762 17 Cuts at: 7258 7411 7596 8041 325 Size: 153 185 445 1498 Fragments arranged by size: 2544 1498 1255 1071 762 519 445 417 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 3833 6345 Size: 274 80 33 51 1443 1829 27 2512 Cuts at: 6345 7460 9172 96 Size: 1115 1712 138 Fragments arranged by size: 2512 1829 1712 1443 1115 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9174 9206 9174 Size: 32 9182 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9214 XbaI T'CTAG_A Cuts at: 6550 6550 Size: 9214 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3364 4661 5728 3364 Size: 1297 1067 6850 Fragments arranged by size: 6850 1297 1067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9214 XmaI C'CCGG_G Cuts at: 1870 5365 6539 1870 Size: 3495 1174 4545 Fragments arranged by size: 4545 3495 1174 XmnI GAAnn'nnTTC Cuts at: 2625 7343 2625 Size: 4718 4496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7022 276 Size: 53 83 186 936 5488 2468 Fragments arranged by size: 5488 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI