(Linear) (Six-base) MAPSORT of: KIEE0300.seq Check: 652 from: 1 to: 4509 KIEE0300.orf With 182 enzymes: SgfI * March 2, 2010 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 123 3558 3995 4193 4509 Size: 123 3435 437 198 316 Fragments arranged by size: 3435 437 316 198 123 AccI GT'mk_AC Cuts at: 0 1259 4091 4509 Size: 1259 2832 418 Fragments arranged by size: 2832 1259 418 Acc65I G'GTAC_C Cuts at: 0 2442 2484 4509 Size: 2442 42 2025 Fragments arranged by size: 2442 2025 42 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 577 685 1220 1306 2218 2285 2296 Size: 559 18 108 535 86 912 67 11 Cuts at: 2296 4509 Size: 2213 Fragments arranged by size: 2213 912 559 535 108 86 67 18 11 AfeI AGC'GCT Cuts at: 0 1709 3023 4509 Size: 1709 1314 1486 Fragments arranged by size: 1709 1486 1314 AflII C'TTAA_G Cuts at: 0 2245 4509 Size: 2245 2264 AflIII A'CryG_T Cuts at: 0 1866 2527 4509 Size: 1866 661 1982 Fragments arranged by size: 1982 1866 661 AhdI GACnn_n'nnGTC Cuts at: 0 2946 4509 Size: 2946 1563 AleI CACnn'nnGTG Cuts at: 0 3256 4509 Size: 3256 1253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 113 2434 3452 4509 Size: 113 2321 1018 1057 Fragments arranged by size: 2321 1057 1018 113 AlwNI CAG_nnn'CTG Cuts at: 0 1413 1549 2584 2968 3602 4509 Size: 1413 136 1035 384 634 907 Fragments arranged by size: 1413 1035 907 634 384 136 ApaI G_GGCC'C Cuts at: 0 1556 3760 4133 4509 Size: 1556 2204 373 376 Fragments arranged by size: 2204 1556 376 373 ApaLI G'TGCA_C Cuts at: 0 1207 4509 Size: 1207 3302 ApoI r'AATT_y Cuts at: 0 232 2603 4509 Size: 232 2371 1906 Fragments arranged by size: 2371 1906 232 AseI AT'TA_AT Cuts at: 0 172 4509 Size: 172 4337 AvaI C'yCGr_G Cuts at: 0 1882 3118 3142 3337 3360 3409 3424 3805 Size: 1882 1236 24 195 23 49 15 381 Cuts at: 3805 4036 4509 Size: 231 473 Fragments arranged by size: 1882 1236 473 381 231 195 49 24 23 15 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2369 2402 4509 Size: 2369 33 2107 Fragments arranged by size: 2369 2107 33 BanI G'GyrC_C Cuts at: 0 108 2442 2484 3148 3983 4395 4509 Size: 108 2334 42 664 835 412 114 Fragments arranged by size: 2334 835 664 412 114 108 42 BanII G_rGCy'C Cuts at: 0 1556 1891 3338 3760 4133 4509 Size: 1556 335 1447 422 373 376 Fragments arranged by size: 1556 1447 422 376 373 335 BbeI G_GCGC'C Cuts at: 0 112 4399 4509 Size: 112 4287 110 Fragments arranged by size: 4287 112 110 BbsI GAAGACnn'nnnn_ Cuts at: 0 287 306 2417 2801 3590 4499 4509 Size: 287 19 2111 384 789 909 10 Fragments arranged by size: 2111 909 789 384 287 19 10 BbvCI CC'TCA_GC Cuts at: 0 3916 3964 4509 Size: 3916 48 545 Fragments arranged by size: 3916 545 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1330 1364 3688 3722 4509 Size: 1330 34 2324 34 787 Fragments arranged by size: 2324 1330 787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 207 4509 Size: 207 4302 BlpI GC'TnA_GC Cuts at: 0 1442 4309 4509 Size: 1442 2867 200 Fragments arranged by size: 2867 1442 200 Bme1580I G_kGCm'C Cuts at: 0 478 1211 1556 3068 3151 3760 3956 4133 Size: 478 733 345 1512 83 609 196 177 Cuts at: 4133 4509 Size: 376 Fragments arranged by size: 1512 733 609 478 376 345 196 177 83 BmrI ACTGGGnnnn_n' Cuts at: 0 1931 2770 4509 Size: 1931 839 1739 Fragments arranged by size: 1931 1739 839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 914 1144 1622 2956 3019 3495 3624 4509 Size: 914 230 478 1334 63 476 129 885 Fragments arranged by size: 1334 914 885 478 476 230 129 63 Bpu10I CC'TnA_GC Cuts at: 0 1414 1774 3916 3964 4162 4198 4509 Size: 1414 360 2142 48 198 36 311 Fragments arranged by size: 2142 1414 360 311 198 48 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 1810 4509 Size: 374 1436 2699 Fragments arranged by size: 2699 1436 374 BsaI GGTCTCn'nnnn_ Cuts at: 0 1004 1182 2668 3931 4102 4509 Size: 1004 178 1486 1263 171 407 Fragments arranged by size: 1486 1263 1004 407 178 171 BsaAI yAC'GTr Cuts at: 0 1869 2528 4509 Size: 1869 659 1981 Fragments arranged by size: 1981 1869 659 BsaHI Gr'CG_yC Cuts at: 0 109 4396 4509 Size: 109 4287 113 Fragments arranged by size: 4287 113 109 BsaWI w'CCGG_w Cuts at: 0 1515 4509 Size: 1515 2994 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1302 1807 2686 2895 3367 3871 4154 4509 Size: 1302 505 879 209 472 504 283 355 Fragments arranged by size: 1302 879 505 504 472 355 283 209 BseYI C'CCAG_C Cuts at: 0 70 303 345 1118 1362 1760 2519 2961 Size: 70 233 42 773 244 398 759 442 Cuts at: 2961 3563 4125 4170 4176 4509 Size: 602 562 45 6 333 Fragments arranged by size: 773 759 602 562 442 398 333 244 233 70 45 42 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 177 760 769 2458 3651 4509 Size: 119 58 583 9 1689 1193 858 Fragments arranged by size: 1689 1193 858 583 119 58 9 BsiEI CG_ry'CG Cuts at: 0 3660 4509 Size: 3660 849 BsiHKAI G_wGCw'C Cuts at: 0 695 1211 2413 3947 4372 4509 Size: 695 516 1202 1534 425 137 Fragments arranged by size: 1534 1202 695 516 425 137 BsmI GAATG_Cn' Cuts at: 0 1327 2256 3621 4509 Size: 1327 929 1365 888 Fragments arranged by size: 1365 1327 929 888 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3262 4509 Size: 3262 1247 Bsp1286I G_dGCh'C Cuts at: 0 478 695 1211 1556 1891 2413 3068 3151 Size: 478 217 516 345 335 522 655 83 Cuts at: 3151 3338 3760 3947 3956 4133 4372 4509 Size: 187 422 187 9 177 239 137 Fragments arranged by size: 655 522 516 478 422 345 335 239 217 187 187 177 137 83 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 123 857 1235 2004 2430 3558 3899 3914 Size: 123 734 378 769 426 1128 341 15 Cuts at: 3914 3995 4193 4313 4509 Size: 81 198 120 196 Fragments arranged by size: 1128 769 734 426 378 341 198 196 123 120 81 15 BsrBI CCG'CTC Cuts at: 0 3139 4166 4509 Size: 3139 1027 343 Fragments arranged by size: 3139 1027 343 BsrDI GCAATG_nn' Cuts at: 0 2416 4430 4509 Size: 2416 2014 79 Fragments arranged by size: 2416 2014 79 BsrFI r'CCGG_y Cuts at: 0 12 61 1358 2813 3078 3232 4359 4509 Size: 12 49 1297 1455 265 154 1127 150 Fragments arranged by size: 1455 1297 1127 265 154 150 49 12 BssSI C'ACGA_G Cuts at: 0 1500 4509 Size: 1500 3009 BstAPI GCAn_nnn'nTGC Cuts at: 0 774 783 1215 3951 4509 Size: 774 9 432 2736 558 Fragments arranged by size: 2736 774 558 432 9 BstBI TT'CG_AA Cuts at: 0 2459 4509 Size: 2459 2050 BstEII G'GTnAC_C Cuts at: 0 658 4509 Size: 658 3851 BstXI CCAn_nnnn'nTGG Cuts at: 0 3519 4125 4509 Size: 3519 606 384 Fragments arranged by size: 3519 606 384 BstYI r'GATC_y Cuts at: 0 1545 3040 4275 4509 Size: 1545 1495 1235 234 Fragments arranged by size: 1545 1495 1235 234 Bsu36I CC'TnA_GG Cuts at: 0 1591 4182 4509 Size: 1591 2591 327 Fragments arranged by size: 2591 1591 327 BtgI C'CryG_G Cuts at: 0 749 1193 3173 3578 3680 4509 Size: 749 444 1980 405 102 829 Fragments arranged by size: 1980 829 749 444 405 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 933 2794 3194 3251 3650 4509 Size: 933 1861 400 57 399 859 Fragments arranged by size: 1861 933 859 400 399 57 BtsI GCAGTG_nn' Cuts at: 0 72 1279 4030 4509 Size: 72 1207 2751 479 Fragments arranged by size: 2751 1207 479 72 DraI TTT'AAA Cuts at: 0 1992 2849 4509 Size: 1992 857 1660 Fragments arranged by size: 1992 1660 857 DraIII CAC_nnn'GTG Cuts at: 0 3951 4509 Size: 3951 558 DrdI GACnn_nn'nnGTC Cuts at: 0 1255 4509 Size: 1255 3254 EaeI y'GGCC_r Cuts at: 0 758 1575 3186 4013 4384 4509 Size: 758 817 1611 827 371 125 Fragments arranged by size: 1611 827 817 758 371 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 39 3366 4340 4496 4509 Size: 39 3327 974 156 13 Fragments arranged by size: 3327 974 156 39 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 577 685 914 1144 1220 1306 1622 Size: 559 18 108 229 230 76 86 316 Cuts at: 1622 2218 2285 2296 2956 3019 3495 3624 4509 Size: 596 67 11 660 63 476 129 885 Fragments arranged by size: 885 660 596 559 476 316 230 229 129 108 86 76 67 63 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 2935 3832 4509 Size: 2935 897 677 Fragments arranged by size: 2935 897 677 EcoO109I rG'GnC_Cy Cuts at: 0 210 1410 1596 2645 3357 3756 3888 4129 Size: 210 1200 186 1049 712 399 132 241 Cuts at: 4129 4130 4509 Size: 1 379 Fragments arranged by size: 1200 1049 712 399 379 241 210 186 132 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 471 795 4509 Size: 471 324 3714 Fragments arranged by size: 3714 471 324 FspI TGC'GCA Cuts at: 0 679 4509 Size: 679 3830 HaeII r_GCGC'y Cuts at: 0 112 1711 1774 3025 4399 4509 Size: 112 1599 63 1251 1374 110 Fragments arranged by size: 1599 1374 1251 112 110 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 274 306 2629 2661 2835 2867 2932 2964 Size: 274 32 2323 32 174 32 65 32 Cuts at: 2964 3117 3149 3313 3331 3345 3363 4252 4284 Size: 153 32 164 18 14 18 889 32 Cuts at: 4284 4509 Size: 225 Fragments arranged by size: 2323 889 274 225 174 164 153 65 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 0 1260 4509 Size: 1260 3249 KasI G'GCGC_C Cuts at: 0 108 4395 4509 Size: 108 4287 114 Fragments arranged by size: 4287 114 108 KpnI G_GTAC'C Cuts at: 0 2446 2488 4509 Size: 2446 42 2021 Fragments arranged by size: 2446 2021 42 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 582 848 1085 1340 1620 3908 4509 Size: 582 266 237 255 280 2288 601 Fragments arranged by size: 2288 601 582 280 266 255 237 MslI CAynn'nnrTG Cuts at: 0 2225 3256 4509 Size: 2225 1031 1253 Fragments arranged by size: 2225 1253 1031 MspA1I CmG'CkG Cuts at: 0 751 1122 1195 1245 1574 3194 3280 3632 Size: 751 371 73 50 329 1620 86 352 Cuts at: 3632 4012 4509 Size: 380 497 Fragments arranged by size: 1620 751 497 380 371 352 329 86 73 50 NaeI GCC'GGC Cuts at: 0 14 63 2815 3080 3234 4509 Size: 14 49 2752 265 154 1275 Fragments arranged by size: 2752 1275 265 154 49 14 NarI GG'CG_CC Cuts at: 0 109 4396 4509 Size: 109 4287 113 Fragments arranged by size: 4287 113 109 NgoMIV G'CCGG_C Cuts at: 0 12 61 2813 3078 3232 4509 Size: 12 49 2752 265 154 1277 Fragments arranged by size: 2752 1277 265 154 49 12 NspI r_CATG'y Cuts at: 0 699 2340 4509 Size: 699 1641 2169 Fragments arranged by size: 2169 1641 699 PasI CC'CwG_GG Cuts at: 0 3288 4509 Size: 3288 1221 PflMI CCAn_nnn'nTGG Cuts at: 0 2634 4509 Size: 2634 1875 PfoI T'CCnGG_A Cuts at: 0 2546 2753 2933 4509 Size: 2546 207 180 1576 Fragments arranged by size: 2546 1576 207 180 PmlI CAC'GTG Cuts at: 0 1869 2528 4509 Size: 1869 659 1981 Fragments arranged by size: 1981 1869 659 PpuMI rG'GwC_Cy Cuts at: 0 210 1596 2645 3357 3888 4509 Size: 210 1386 1049 712 531 621 Fragments arranged by size: 1386 1049 712 621 531 210 PshAI GACnn'nnGTC Cuts at: 0 2573 4509 Size: 2573 1936 PspOMI G'GGCC_C Cuts at: 0 1552 3756 4129 4509 Size: 1552 2204 373 380 Fragments arranged by size: 2204 1552 380 373 PstI C_TGCA'G Cuts at: 0 123 728 972 1238 4509 Size: 123 605 244 266 3271 Fragments arranged by size: 3271 605 266 244 123 PvuI CG_AT'CG Cuts at: 0 3660 4509 Size: 3660 849 PvuII CAG'CTG Cuts at: 0 1122 3280 4509 Size: 1122 2158 1229 Fragments arranged by size: 2158 1229 1122 SacII CC_GC'GG Cuts at: 0 752 1196 4509 Size: 752 444 3313 Fragments arranged by size: 3313 752 444 SalI G'TCGA_C Cuts at: 0 1258 4509 Size: 1258 3251 SanDI GG'GwC_CC Cuts at: 0 1596 4509 Size: 1596 2913 ScaI AGT'ACT Cuts at: 0 4342 4509 Size: 4342 167 SfcI C'TryA_G Cuts at: 0 119 371 724 968 1234 4509 Size: 119 252 353 244 266 3275 Fragments arranged by size: 3275 353 266 252 244 119 SfoI GGC'GCC Cuts at: 0 110 4397 4509 Size: 110 4287 112 Fragments arranged by size: 4287 112 110 SgrAI Cr'CCGG_yG Cuts at: 0 12 4509 Size: 12 4497 SmaI CCC'GGG Cuts at: 0 3362 4509 Size: 3362 1147 SmlI C'TyrA_G Cuts at: 0 389 1789 2245 4509 Size: 389 1400 456 2264 Fragments arranged by size: 2264 1400 456 389 SpeI A'CTAG_T Cuts at: 0 3060 4509 Size: 3060 1449 SspI AAT'ATT Cuts at: 0 170 336 588 1952 4509 Size: 170 166 252 1364 2557 Fragments arranged by size: 2557 1364 252 170 166 StyI C'CwwG_G Cuts at: 0 3380 4509 Size: 3380 1129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 646 994 1411 3955 4474 4509 Size: 646 348 417 2544 519 35 Fragments arranged by size: 2544 646 519 417 348 35 TatI w'GTAC_w Cuts at: 0 1801 1828 4340 4509 Size: 1801 27 2512 169 Fragments arranged by size: 2512 1801 169 27 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1359 2656 3723 4509 Size: 1359 1297 1067 786 Fragments arranged by size: 1359 1297 1067 786 XmaI C'CCGG_G Cuts at: 0 3360 4509 Size: 3360 1149 XmnI GAAnn'nnTTC Cuts at: 0 620 4509 Size: 620 3889 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuI PvuII SacII SalI SanDI ScaI SfcI SfoI SgrAI SmaI SmlI SpeI SspI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AgeI AloI AscI AsiSI AvrII BamHI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaBI BsaXI BsiWI BspEI BspHI BsrGI BssHII BstZ17I ClaI CspCI EagI EarI EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI RsrII SacI SapI SbfI SexAI SfiI SgfI SnaBI SphI SrfI StuI SwaI TstI Tth111I XbaI XhoI ZraI