(Circular) (Six-base) MAPSORT of: pFN21AB8996.seq Check: 5978 from: 1 to: 5926 pFN21AB8996 5926 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 3062 1166 Size: 1896 4030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 2515 2679 2776 3669 5241 2515 Size: 164 97 893 1572 3200 Fragments arranged by size: 3200 1572 893 164 97 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2146 2398 2469 3251 1870 Size: 73 203 252 71 782 4545 Fragments arranged by size: 4545 782 252 203 73 71 BamHI G'GATC_C Cuts at: 2141 3256 3623 2141 Size: 1115 367 4444 Fragments arranged by size: 4444 1115 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2402 2785 2862 3247 4702 Size: 325 217 627 615 383 77 385 1455 Cuts at: 4702 618 Size: 1842 Fragments arranged by size: 1842 1455 627 615 385 383 325 217 77 BanII G_rGCy'C Cuts at: 1391 2090 2205 3245 1391 Size: 699 115 1040 4072 Fragments arranged by size: 4072 1040 699 115 BbeI G_GCGC'C Cuts at: 2406 2406 Size: 5926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2852 2886 4117 4151 1903 Size: 34 915 34 1231 34 3678 Fragments arranged by size: 3678 1231 915 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2711 2949 3023 Size: 107 122 71 631 605 1039 238 74 Cuts at: 3023 4537 136 Size: 1514 1525 Fragments arranged by size: 1525 1514 1039 631 605 238 122 107 74 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 3134 3343 3134 Size: 209 5717 Bme1580I G_kGCm'C Cuts at: 1790 3195 3987 5143 1790 Size: 1405 792 1156 2573 Fragments arranged by size: 2573 1405 1156 792 BmgBI CAC'GTC Cuts at: 1318 2752 3190 1318 Size: 1434 438 4054 Fragments arranged by size: 4054 1434 438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2591 2906 4586 1239 Size: 1010 342 315 1680 2579 Fragments arranged by size: 2579 1680 1010 342 315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2272 2651 4051 4916 5214 2272 Size: 379 1400 865 298 2984 Fragments arranged by size: 2984 1400 865 379 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 2403 3733 4115 Size: 53 83 186 936 575 295 1330 382 Cuts at: 4115 4750 275 Size: 635 1451 Fragments arranged by size: 1451 1330 936 635 575 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2455 3037 3224 1186 Size: 1027 242 582 187 3888 Fragments arranged by size: 3888 1027 582 242 187 BseYI C'CCAG_C Cuts at: 2029 2068 2848 3013 5129 5625 2029 Size: 39 780 165 2116 496 2330 Fragments arranged by size: 2330 2116 780 496 165 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2774 2858 1163 Size: 573 582 456 84 4231 Fragments arranged by size: 4231 582 573 456 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2023 2061 2209 2362 3376 4137 Size: 275 186 22 38 148 153 1014 761 Cuts at: 4137 5165 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 1014 761 275 186 153 148 38 22 BsiHKAI G_wGCw'C Cuts at: 2205 3140 3987 4072 5143 2205 Size: 935 847 85 1071 2988 Fragments arranged by size: 2988 1071 935 847 85 BsmI GAATG_Cn' Cuts at: 2868 3120 3437 3530 2868 Size: 252 317 93 5264 Fragments arranged by size: 5264 317 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2205 3140 3195 3245 3987 4072 Size: 399 300 115 935 55 50 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 935 742 399 300 115 85 55 50 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2725 2734 3813 1394 Size: 429 902 9 1079 3507 Fragments arranged by size: 3507 1079 902 429 9 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2274 2583 2788 2820 2978 4570 Size: 110 250 214 309 205 32 158 1592 Cuts at: 4570 4757 1700 Size: 187 2869 Fragments arranged by size: 2869 1592 309 250 214 205 187 158 110 32 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 794 Size: 1502 4424 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2141 2370 3256 3623 4008 4025 5477 5563 Size: 169 229 886 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 886 385 367 346 229 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 2301 2076 Size: 225 5701 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2626 2781 513 Size: 552 939 306 316 155 3658 Fragments arranged by size: 3658 939 552 316 306 155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2907 487 Size: 683 56 1681 3506 Fragments arranged by size: 3506 1681 683 56 BtsI GCAGTG_nn' Cuts at: 2762 3442 4236 4256 2762 Size: 680 794 20 4432 Fragments arranged by size: 4432 794 680 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DraIII CAC_nnn'GTG Cuts at: 2296 2296 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2192 2206 Size: 54 1425 50 275 18 228 134 14 Cuts at: 2206 2359 2386 2410 2778 2896 3373 4262 5891 Size: 153 27 24 368 118 477 889 1629 Cuts at: 5891 8 Size: 43 Fragments arranged by size: 1629 1425 889 477 368 275 228 153 134 118 54 50 43 27 24 18 14 EagI C'GGCC_G Cuts at: 1537 1812 2058 2206 2359 3373 1537 Size: 275 246 148 153 1014 4090 Fragments arranged by size: 4090 1014 275 246 153 148 EarI CTCTTCn'nnn_ Cuts at: 1353 2648 2805 3856 1353 Size: 1295 157 1051 3423 Fragments arranged by size: 3423 1295 1051 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2465 2602 2930 4513 4887 5033 Size: 1064 138 876 137 328 1583 374 146 Cuts at: 5033 387 Size: 1280 Fragments arranged by size: 1583 1280 1064 876 374 328 146 138 137 EcoICRI GAG'CTC Cuts at: 2203 2203 Size: 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2591 2906 3989 4586 5373 5641 Size: 393 617 342 315 1083 597 787 268 Cuts at: 5641 1239 Size: 1524 Fragments arranged by size: 1524 1083 787 617 597 393 342 315 268 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2573 Size: 5926 EcoO109I rG'GnC_Cy Cuts at: 2072 3031 2072 Size: 959 4967 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FseI GG_CCGG'CC Cuts at: 2278 2278 Size: 5926 FspI TGC'GCA Cuts at: 2327 4432 2327 Size: 2105 3821 HaeII r_GCGC'y Cuts at: 1678 2030 2406 5073 1678 Size: 352 376 2667 2531 Fragments arranged by size: 2667 2531 376 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373 Size: 32 135 32 2995 32 42 32 2626 Fragments arranged by size: 2995 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2402 2402 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 5040 5224 1305 Size: 1215 2520 184 2007 Fragments arranged by size: 2520 2007 1215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3062 3146 3203 3884 4243 4402 518 Size: 648 1896 84 57 681 359 159 2042 Fragments arranged by size: 2042 1896 681 648 359 159 84 57 MspA1I CmG'CkG Cuts at: 1606 2676 2766 3009 3342 4019 5167 5412 1606 Size: 1070 90 243 333 677 1148 245 2120 Fragments arranged by size: 2120 1148 1070 677 333 245 243 90 NaeI GCC'GGC Cuts at: 2062 2276 2585 2062 Size: 214 309 5403 Fragments arranged by size: 5403 309 214 NarI GG'CG_CC Cuts at: 2403 2403 Size: 5926 NcoI C'CATG_G Cuts at: 513 1065 2004 2310 513 Size: 552 939 306 4129 Fragments arranged by size: 4129 939 552 306 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 2060 2274 2583 2060 Size: 214 309 5403 Fragments arranged by size: 5403 309 214 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3085 3284 4829 1132 Size: 97 1856 199 1545 2229 Fragments arranged by size: 2229 1856 1545 199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2538 2776 1108 Size: 1430 238 4258 Fragments arranged by size: 4258 1430 238 PfoI T'CCnGG_A Cuts at: 2226 2568 2613 2226 Size: 342 45 5539 Fragments arranged by size: 5539 342 45 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3031 3031 Size: 5926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 2265 3278 830 Size: 1435 1013 3478 Fragments arranged by size: 3478 1435 1013 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2766 3009 2766 Size: 243 5683 SacI G_AGCT'C Cuts at: 2205 2205 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SanDI GG'GwC_CC Cuts at: 3031 3031 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2261 3274 4409 5090 5281 826 Size: 219 1216 1013 1135 681 191 1471 Fragments arranged by size: 1471 1216 1135 1013 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2711 2711 Size: 5926 SfoI GGC'GCC Cuts at: 2404 2404 Size: 5926 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2148 2400 2471 3253 1872 Size: 276 252 71 782 4545 Fragments arranged by size: 4545 782 276 252 71 SmlI C'TyrA_G Cuts at: 820 1017 1943 2251 2666 4030 4931 5193 820 Size: 197 926 308 415 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 415 308 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3085 3284 1132 Size: 1953 199 3774 Fragments arranged by size: 3774 1953 199 SrfI GCCC'GGGC Cuts at: 2400 2400 Size: 5926 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2081 2714 2883 1517 Size: 564 633 169 4560 Fragments arranged by size: 4560 633 564 169 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 3365 513 Size: 552 939 306 1055 3074 Fragments arranged by size: 3074 1055 939 552 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2107 2989 3953 3970 4123 4308 4753 Size: 1255 527 882 964 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 964 882 527 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 3153 3168 Size: 274 80 33 51 1443 630 546 15 Cuts at: 3168 4172 5884 96 Size: 1004 1712 138 Fragments arranged by size: 1712 1443 1004 630 546 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2983 3015 5886 5918 2983 Size: 32 2871 32 2991 Fragments arranged by size: 2991 2871 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3202 3202 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2146 2398 2469 3251 1870 Size: 276 252 71 782 4545 Fragments arranged by size: 4545 782 276 252 71 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SwaI