Restriction map for
pFN21AE0228
##################################
# NO CUT ENZYMES ( 196 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII ItaI KasI KpnI
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I TseI TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 209 AAATTT [GA]^AATT[CT]
AluI 165 AGCT AG^CT
ApoI 209 AAATTT [GA]^AATT[CT]
AspI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 161 CCCGAG C^[CT]CG[GA]G
BanII 194 GGGCTC G[GA]GC[CT]^C
BbsI 235 GAAGAC GAAGAC(2/6)
BlpI 102 GCTGAGC GC^T[ACGT]AGC
BmyI 194 GGGCTC G[AGT]GC[ACT]^C
BpmI 197 CTCCAG CTGGAG(16/14)
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
BpuAI 235 GAAGAC GAAGAC(2/6)
BsmAI 91 GAGAC GTCTC(1/5)
BsoBI 161 CCCGAG C^[CT]CG[GA]G
Bsp1286I 194 GGGCTC G[AGT]GC[ACT]^C
BspMI 271 GCAGGT ACCTGC(4/8)
BsrI 75 ACTGG ACTGG(1/-1)
Cac8I 202 GCATGC GC[ACGT]^[ACGT]GC
CelII 102 GCTGAGC GC^T[ACGT]AGC
DraII 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoO109I 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 255 GACGC GACGC(5/10)
MaeIII 242 GTCAC ^GT[ACGT]AC
MwoI 206 GCCAAATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 203 CATG CATG^
NlaIV 195 GGCTCC GG[ACGT]^[ACGT]CC
NspI 202 GCATGC [GA]CATG^[CT]
PflFI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 277 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 11 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 318 AGTACT AGT^ACT
SfaNI 215 GCATC GCATC(5/9)
SfcI 143 CTACAG C^T[GA][CT]AG
SphI 202 GCATGC GCATG^C
TfiI 307 GATTC G^A[AT]TC
Tsp45I 242 GTCAC ^GT[GC]AC
TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 277 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 59 GTAC GT^AC
319 GTAC
AvaII 12 GGTCC G^G[AT]CC
274 GGTCC
BsiYI 42 CCGTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
277 CCAACATCTGG
BslI 42 CCGTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
277 CCAACATCTGG
BsmFI 253 GGGAC GGGAC(10/14)
261 GGGAC
BssKI 108 CCAGG ^CC[ACGT]GG
246 CCTGG
BstNI 108 CCAGG CC^[AT]GG
246 CCTGG
DdeI 103 CTGAG C^T[ACGT]AG
267 CTCAG
EarI 24 GAAGAG CTCTTC(1/4)
112 GAAGAG
EcoRII 108 CCAGG ^CC[AT]GG
246 CCTGG
HaeIII 3 GGCC GG^CC
159 GGCC
Ksp632I 24 GAAGAG CTCTTC(1/4)
112 GAAGAG
MvaI 108 CCAGG CC^[AT]GG
246 CCTGG
PleI 175 GACTC GAGTC(4/5)
263 GACTC
RsaI 59 GTAC GT^AC
319 GTAC
ScrFI 108 CCAGG CC^[ACGT]GG
246 CCTGG
Tsp509I 83 AATT ^AATT
210 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
Cfr13I HinfI HphI MboII Sau96I