Restriction map for
pFN21AF0190
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MroI MseI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 194 GTAGAC GT^[AC][GT]AC
AcsI 1285 GAATTC [GA]^AATT[CT]
AocI 1438 CCTGAGG CC^T[ACGT]AGG
Aor51HI 14 AGCGCT AGC^GCT
ApoI 1285 GAATTC [GA]^AATT[CT]
BbsI 747 GAAGAC GAAGAC(2/6)
BcnI 1246 CCGGG CC^[GC]GG
BglI 673 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 747 GAAGAC GAAGAC(2/6)
BsaAI 429 CACGTA [CT]AC^GT[GA]
BsaWI 1365 ACCGGA [AT]^CCGG[AT]
BsmI 481 GCATTC GAATGC(1/-1)
Bsp1407I 275 TGTACA T^GTACA
BspMI 646 ACCTGC ACCTGC(4/8)
BsrBI 50 CCGCTC CCGCTC(-3/-3)
BsrFI 372 GCCGGC [GA]^CCGG[CT]
BsrGI 275 TGTACA T^GTACA
BstXI 1172 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1438 CCTGAGG CC^T[ACGT]AGG
Cfr10I 372 GCCGGC [GA]^CCGG[CT]
DraIII 820 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 14 AGCGCT AGC^GCT
Eco81I 1438 CCTGAGG CC^T[ACGT]AGG
EcoRI 1285 GAATTC G^AATTC
HaeII 14 AGCGCT [GA]GCGC^[CT]
HphI 395 TCACC GGTGA(8/7)
MluI 294 ACGCGT A^CGCGT
NaeI 372 GCCGGC GCC^GGC
NciI 1246 CCGGG CC^[GC]GG
NcoI 531 CCATGG C^CATGG
NgoMIV 372 GCCGGC G^CCGGC
NspI 449 GCATGC [GA]CATG^[CT]
PaeR7I 917 CTCGAG C^TCGAG
PflMI 133 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 848 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 43 CTGCAG CTGCA^G
SapI 1312 GAAGAGC GCTCTTC(1/4)
SfcI 43 CTGCAG C^T[GA][CT]AG
SmlI 917 CTCGAG C^T[CT][GA]AG
SphI 449 GCATGC GCATG^C
SspBI 275 TGTACA T^GTACA
TaqI 918 TCGA T^CGA
TthHB8I 918 TCGA T^CGA
Van91I 133 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 917 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 205 CGCG CG^CG
295 CGCG
AcyI 912 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
AflIII 294 ACGCGT A^C[GA][CT]GT
605 ACACGT
AlwI 411 GATCC GGATC(4/5)
462 GGATC
BalI 534 TGGCCA TGG^CCA
1553 TGGCCA
BanI 1259 GGCACC G^G[CT][GA]CC
1354 GGCACC
BsaHI 912 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
BstUI 205 CGCG CG^CG
295 CGCG
BstYI 410 AGATCC [GA]^GATC[CT]
462 GGATCT
CfoI 15 GCGC GCG^C
204 GCGC
DsaI 531 CCATGG C^C[GA][CT]GG
790 CCACGG
EaeI 534 TGGCCA [CT]^GGCC[GA]
1553 TGGCCA
HhaI 15 GCGC GCG^C
204 GCGC
Hin1I 912 GACGCC G[GA]^CG[CT]C
1009 GGCGTC
HinP1I 15 GCGC G^CGC
204 GCGC
MaeII 430 ACGT A^CGT
607 ACGT
MflI 410 AGATCC [GA]^GATC[CT]
462 GGATCT
MluNI 534 TGGCCA TGG^CCA
1553 TGGCCA
MscI 534 TGGCCA TGG^CCA
1553 TGGCCA
MvnI 205 CGCG CG^CG
295 CGCG
SacI 180 GAGCTC GAGCT^C
1315 GAGCTC
SfaNI 786 GATGC GCATC(5/9)
1483 GATGC
XhoII 410 AGATCC [GA]^GATC[CT]
462 GGATCT
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI StyI TfiI TseI
Tsp45I Tsp509I TspRI XcmI