Restriction map for
pFN21AF0190
(Linear) (Six-base) MAPSORT of: FLJ00190.seq Check: 1741 from: 1 to: 1560
FLJ00190.orf
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 655 1560
Size: 655 905
AccI GT'mk_AC
Cuts at: 0 195 1560
Size: 195 1365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 280 487 1139 1390 1560
Size: 93 187 207 652 251 170
Fragments arranged by size:
652 251 207 187 170 93
AfeI AGC'GCT
Cuts at: 0 16 1560
Size: 16 1544
AflIII A'CryG_T
Cuts at: 0 294 605 1560
Size: 294 311 955
Fragments arranged by size:
955 311 294
AlwNI CAG_nnn'CTG
Cuts at: 0 273 1166 1413 1560
Size: 273 893 247 147
Fragments arranged by size:
893 273 247 147
ApaI G_GGCC'C
Cuts at: 0 11 25 798 930 1560
Size: 11 14 773 132 630
Fragments arranged by size:
773 630 132 14 11
ApoI r'AATT_y
Cuts at: 0 1285 1560
Size: 1285 275
AvaI C'yCGr_G
Cuts at: 0 10 917 1299 1560
Size: 10 907 382 261
Fragments arranged by size:
907 382 261 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 700 733 1560
Size: 700 33 827
Fragments arranged by size:
827 700 33
BanI G'GyrC_C
Cuts at: 0 1259 1354 1560
Size: 1259 95 206
Fragments arranged by size:
1259 206 95
BanII G_rGCy'C
Cuts at: 0 11 25 184 798 930 1116 1319 1560
Size: 11 14 159 614 132 186 203 241
Fragments arranged by size:
614 241 203 186 159 132 14 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 754 1560
Size: 754 806
BglI GCCn_nnn'nGGC
Cuts at: 0 679 1560
Size: 679 881
Bme1580I G_kGCm'C
Cuts at: 0 11 25 798 930 1076 1262 1357 1560
Size: 11 14 773 132 146 186 95 203
Fragments arranged by size:
773 203 186 146 132 95 14 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 687 1390 1560
Size: 687 703 170
Fragments arranged by size:
703 687 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 541 679 1052 1560
Size: 38 503 138 373 508
Fragments arranged by size:
508 503 373 138 38
BsaAI yAC'GTr
Cuts at: 0 431 1560
Size: 431 1129
BsaHI Gr'CG_yC
Cuts at: 0 913 1010 1560
Size: 913 97 550
Fragments arranged by size:
913 550 97
BsaWI w'CCGG_w
Cuts at: 0 1365 1560
Size: 1365 195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 51 81 294 324 1321 1351 1560
Size: 51 30 213 30 997 30 209
Fragments arranged by size:
997 213 209 51 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 994 997 1006 1106 1128 1176 1326
Size: 421 573 3 9 100 22 48 150
Cuts at: 1326 1456 1465 1560
Size: 130 9 95
Fragments arranged by size:
573 421 150 130 100 95 48 22
9 9 3
BseYI C'CCAG_C
Cuts at: 0 17 313 841 930 1166 1201 1424 1493
Size: 17 296 528 89 236 35 223 69
Cuts at: 1493 1560
Size: 67
Fragments arranged by size:
528 296 236 223 89 69 67 35
17
BsiHKAI G_wGCw'C
Cuts at: 0 184 395 1061 1319 1510 1560
Size: 184 211 666 258 191 50
Fragments arranged by size:
666 258 211 191 184 50
BsmI GAATG_Cn'
Cuts at: 0 481 1560
Size: 481 1079
Bsp1286I G_dGCh'C
Cuts at: 0 11 25 184 395 798 930 1061 1076
Size: 11 14 159 211 403 132 131 15
Cuts at: 1076 1116 1262 1319 1357 1510 1560
Size: 40 146 57 38 153 50
Fragments arranged by size:
403 211 159 153 146 132 131 57
50 40 38 15 14 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 655 1560
Size: 655 905
BsrBI CCG'CTC
Cuts at: 0 52 1560
Size: 52 1508
BsrFI r'CCGG_y
Cuts at: 0 372 1560
Size: 372 1188
BsrGI T'GTAC_A
Cuts at: 0 275 1560
Size: 275 1285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1179 1560
Size: 1179 381
BstYI r'GATC_y
Cuts at: 0 410 462 1560
Size: 410 52 1098
Fragments arranged by size:
1098 410 52
Bsu36I CC'TnA_GG
Cuts at: 0 1439 1560
Size: 1439 121
BtgI C'CryG_G
Cuts at: 0 531 790 1560
Size: 531 259 770
Fragments arranged by size:
770 531 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 683 1496 1560
Size: 683 813 64
Fragments arranged by size:
813 683 64
BtsI GCAGTG_nn'
Cuts at: 0 579 1560
Size: 579 981
DraIII CAC_nnn'GTG
Cuts at: 0 825 1560
Size: 825 735
EaeI y'GGCC_r
Cuts at: 0 534 1553 1560
Size: 534 1019 7
Fragments arranged by size:
1019 534 7
EarI CTCTTCn'nnn_
Cuts at: 0 209 971 1307 1409 1442 1560
Size: 209 762 336 102 33 118
Fragments arranged by size:
762 336 209 118 102 33
EcoICRI GAG'CTC
Cuts at: 0 182 1317 1560
Size: 182 1135 243
Fragments arranged by size:
1135 243 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 93 280 487 541 679 1052 1139
Size: 38 55 187 207 54 138 373 87
Cuts at: 1139 1390 1560
Size: 251 170
Fragments arranged by size:
373 251 207 187 170 138 87 55
54 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 967 1194 1560
Size: 43 924 227 366
Fragments arranged by size:
924 366 227 43
EcoO109I rG'GnC_Cy
Cuts at: 0 7 21 22 62 270 663 795 926
Size: 7 14 1 40 208 393 132 131
Cuts at: 926 927 960 1205 1390 1560
Size: 1 33 245 185 170
Fragments arranged by size:
393 245 208 185 170 132 131 40
33 14 7 1 1
EcoRI G'AATT_C
Cuts at: 0 1285 1560
Size: 1285 275
HaeII r_GCGC'y
Cuts at: 0 18 1560
Size: 18 1542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 294 326 1318 1350 1560
Size: 51 32 211 32 992 32 210
Fragments arranged by size:
992 211 210 51 32 32 32
MluI A'CGCG_T
Cuts at: 0 294 1560
Size: 294 1266
MscI TGG'CCA
Cuts at: 0 536 1555 1560
Size: 536 1019 5
Fragments arranged by size:
1019 536 5
MspA1I CmG'CkG
Cuts at: 0 438 565 777 1493 1560
Size: 438 127 212 716 67
Fragments arranged by size:
716 438 212 127 67
NaeI GCC'GGC
Cuts at: 0 374 1560
Size: 374 1186
NcoI C'CATG_G
Cuts at: 0 531 1560
Size: 531 1029
NgoMIV G'CCGG_C
Cuts at: 0 372 1560
Size: 372 1188
NspI r_CATG'y
Cuts at: 0 453 1560
Size: 453 1107
PasI CC'CwG_GG
Cuts at: 0 133 329 1109 1384 1560
Size: 133 196 780 275 176
Fragments arranged by size:
780 275 196 176 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 1560
Size: 139 1421
PfoI T'CCnGG_A
Cuts at: 0 1307 1560
Size: 1307 253
PpuMI rG'GwC_Cy
Cuts at: 0 270 663 960 1205 1390 1560
Size: 270 393 297 245 185 170
Fragments arranged by size:
393 297 270 245 185 170
PshAI GACnn'nnGTC
Cuts at: 0 852 1560
Size: 852 708
PspOMI G'GGCC_C
Cuts at: 0 7 21 794 926 1560
Size: 7 14 773 132 634
Fragments arranged by size:
773 634 132 14 7
PspXI vC'TCGA_Gb
Cuts at: 0 917 1560
Size: 917 643
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 268 300 1560
Size: 268 32 1260
Fragments arranged by size:
1260 268 32
PstI C_TGCA'G
Cuts at: 0 47 1560
Size: 47 1513
PvuII CAG'CTG
Cuts at: 0 438 565 1493 1560
Size: 438 127 928 67
Fragments arranged by size:
928 438 127 67
SacI G_AGCT'C
Cuts at: 0 184 1319 1560
Size: 184 1135 241
Fragments arranged by size:
1135 241 184
SanDI GG'GwC_CC
Cuts at: 0 1390 1560
Size: 1390 170
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 1560
Size: 1307 253
SfcI C'TryA_G
Cuts at: 0 43 1560
Size: 43 1517
SmlI C'TyrA_G
Cuts at: 0 917 1560
Size: 917 643
SphI G_CATG'C
Cuts at: 0 453 1560
Size: 453 1107
StyI C'CwwG_G
Cuts at: 0 230 531 1239 1292 1560
Size: 230 301 708 53 268
Fragments arranged by size:
708 301 268 230 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 412 952 1525 1534 1560
Size: 321 91 540 573 9 26
Fragments arranged by size:
573 540 321 91 26 9
TatI w'GTAC_w
Cuts at: 0 275 1560
Size: 275 1285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1028 1060 1560
Size: 1028 32 500
Fragments arranged by size:
1028 500 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 674 1116 1246 1560
Size: 674 442 130 314
Fragments arranged by size:
674 442 314 130
XhoI C'TCGA_G
Cuts at: 0 917 1560
Size: 917 643
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbsI BglI Bme1580I BmrI
BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MluI MscI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SanDI SapI SfcI SmlI SphI StyI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MmeI MslI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XmaI XmnI
ZraI