Restriction map for
pFN21AB6367
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MfeI MluI MroI MseI MslI
MunI MwoI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I TseI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 192 CGCG CG^CG
AcsI 679 GAATTT [GA]^AATT[CT]
Alw44I 340 GTGCAC G^TGCAC
AlwI 604 GATCC GGATC(4/5)
AlwNI 109 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 340 GTGCAC G^TGCAC
ApoI 679 GAATTT [GA]^AATT[CT]
BalI 206 TGGCCA TGG^CCA
BanII 16 GAGCTC G[GA]GC[CT]^C
BbvCI 219 GCTGAGG CCTCAGC(-5/-2)
BcnI 378 CCGGG CC^[GC]GG
BsaBI 657 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 369 CTGCAC GTGCAG(16/14)
Bsp1407I 486 TGTACA T^GTACA
BsrDI 50 GCAATG GCAATG(2/0)
BsrGI 486 TGTACA T^GTACA
BstUI 192 CGCG CG^CG
BstYI 603 AGATCC [GA]^GATC[CT]
DraII 110 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 209 CCATGG C^C[GA][CT]GG
EcoO109I 110 AGGACCT [GA]G^G[ACGT]CC[CT]
HapII 378 CCGG C^CGG
HgaI 193 GCGTC GACGC(5/10)
HindIII 396 AAGCTT A^AGCTT
HpaII 378 CCGG C^CGG
MamI 657 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 603 AGATCC [GA]^GATC[CT]
MluNI 206 TGGCCA TGG^CCA
MscI 206 TGGCCA TGG^CCA
MspA1I 589 CAGCTG C[AC]G^C[GT]G
MspI 378 CCGG C^CGG
MvnI 192 CGCG CG^CG
NciI 378 CCGGG CC^[GC]GG
NcoI 209 CCATGG C^CATGG
NdeI 416 CATATG CA^TATG
NspI 734 ACATGC [GA]CATG^[CT]
PflMI 108 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 110 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 589 CAGCTG CAG^CTG
SacI 16 GAGCTC GAGCT^C
SspBI 486 TGTACA T^GTACA
TaqI 314 TCGA T^CGA
TthHB8I 314 TCGA T^CGA
Van91I 108 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 603 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BpmI 439 CTCCAG CTGGAG(16/14)
592 CTGGAG
DpnII 359 GATC ^GATC
604 GATC
EaeI 206 TGGCCA [CT]^GGCC[GA]
765 CGGCCA
EcoT14I 209 CCATGG C^C[AT][AT]GG
524 CCTTGG
HphI 402 GGTGA GGTGA(8/7)
785 TCACC
MboI 359 GATC ^GATC
604 GATC
NdeII 359 GATC ^GATC
604 GATC
NlaIV 234 GGTTCC GG[ACGT]^[ACGT]CC
754 GGGACC
PleI 382 GACTC GAGTC(4/5)
685 GAGTC
Sau3AI 359 GATC ^GATC
604 GATC
SfcI 168 CTACAG C^T[GA][CT]AG
586 CTACAG
SmlI 532 CTCAAG C^T[CT][GA]AG
722 CTCAAG
StyI 209 CCATGG C^C[AT][AT]GG
524 CCTTGG
TfiI 152 GAATC G^A[AT]TC
543 GAATC
XcmI 23 CCAGTGAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
581 CCACTCTACAGCTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbsI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
NlaIII RsaI Sau96I ScrFI SfaNI
Tsp509I TspRI