Restriction map for pFN21AB6367
 (Circular) (Six-base) MAPSORT of: pFN21AB6367.seq  Check: 237  from: 1  to: 5512



pFN21AB6367  5512 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:40  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3322     278 
   Size:         53      83     186     936    1786    2468

  Fragments arranged by size: 

                2468    1786     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2855    1937 
   Size:        918    4594
 
Acc65I G'GTAC_C

Cuts at:   2833    2833 
   Size:       5512
 
AclI AA'CG_TT

Cuts at:   1563    3639    4012    1563 
   Size:       2076     373    3063

  Fragments arranged by size: 

                3063    2076     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2170    3575    4959    5227    1632 
   Size:        538    1405    1384     268    1917

  Fragments arranged by size: 

                1917    1405    1384     538     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5315
 
AflIII A'CryG_T

Cuts at:   1203    4411    5388    1203 
   Size:       3208     977    1327

  Fragments arranged by size: 

                3208    1327     977
 
AgeI A'CCGG_T

Cuts at:   4343    4343 
   Size:       5512
 
AhdI GACnn_n'nnGTC

Cuts at:   4241    4241 
   Size:       5512
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5512
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5512
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2054    2086    2054 
   Size:         32    5480
 
AlwNI CAG_nnn'CTG

Cuts at:   2119    3255    4827    2119 
   Size:       1136    1572    2804

  Fragments arranged by size: 

                2804    1572    1136
 
ApaLI G'TGCA_C

Cuts at:   2345    3569    4725    2345 
   Size:       1224    1156    3132

  Fragments arranged by size: 

                3132    1224    1156
 
ApoI r'AATT_y

Cuts at:   1493    2684    2821    3047    3199    5308    1493 
   Size:       1191     137     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1191     226     152     137
 
AseI AT'TA_AT

Cuts at:    160    1032    4066     160 
   Size:        872    3034    1606

  Fragments arranged by size: 

                3034    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5512
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2837    1870 
   Size:         73     894    4545

  Fragments arranged by size: 

                4545     894      73
 
BamHI G'GATC_C

Cuts at:   2842    3209    2842 
   Size:        367    5145
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2833    4288     618 
   Size:        325     217     627    1046    1455    1842

  Fragments arranged by size: 

                1842    1455    1046     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2025    2831    1391 
   Size:        634     806    4072

  Fragments arranged by size: 

                4072     806     634
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2644    2740    2799     928 
   Size:        971     745      96      59    3641

  Fragments arranged by size: 

                3641     971     745      96      59
 
BbvCI CC'TCA_GC

Cuts at:   2225    2225 
   Size:       5512
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3703    3737    1903 
   Size:         34    1766      34    3678

  Fragments arranged by size: 

                3678    1766      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3404    4614    3404 
   Size:       1210    4302
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5512
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4123     136 
   Size:        107     122      71     631     605    2451    1525

  Fragments arranged by size: 

                2451    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5507    5507 
   Size:       5512
 
BlpI GC'TnA_GC

Cuts at:   2929    2929 
   Size:       5512
 
Bme1580I G_kGCm'C

Cuts at:   1790    2349    3573    4729    1790 
   Size:        559    1224    1156    2573

  Fragments arranged by size: 

                2573    1224    1156     559
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5512
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2692    3243    4201 
   Size:        416      53      83     399      24    1275     551     958

Cuts at:   4201     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1275     958     551     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5512
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2429    2618    4172    1239 
   Size:       1190     189    1554    2579

  Fragments arranged by size: 

                2579    1554    1190     189
 
Bpu10I CC'TnA_GC

Cuts at:   2019    2225    2273    2019 
   Size:        206      48    5258

  Fragments arranged by size: 

                5258     206      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2522    2712    3637    4502    4800    2522 
   Size:        190     925     865     298    3234

  Fragments arranged by size: 

                3234     925     865     298     190
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4175     882 
   Size:        617    2676    2219

  Fragments arranged by size: 

                2676    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3303     493 
   Size:        711    2099    2702

  Fragments arranged by size: 

                2702    2099     711
 
BsaBI GATnn'nnATC

Cuts at:   2666    5506    2666 
   Size:       2840    2672
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3319    3701    4336     275 
   Size:         53      83     186     936    1786     382     635    1451

  Fragments arranged by size: 

                1786    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3945    4343    4617    4764    5175    3945 
   Size:        398     274     147     411    4282

  Fragments arranged by size: 

                4282     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5287

  Fragments arranged by size: 

                5287     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5512
 
BseYI C'CCAG_C

Cuts at:   4715    5211    4715 
   Size:        496    5016
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2359    1163 
   Size:        573     623    4316

  Fragments arranged by size: 

                4316     623     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2962    3723    4751    1540 
   Size:        275     186     961     761    1028    2301

  Fragments arranged by size: 

                2301    1028     961     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2025    2108    2279    2349    3573    3658    4729    2025 
   Size:         83     171      70    1224      85    1071    2808

  Fragments arranged by size: 

                2808    1224    1071     171      85      83      70
 
BsmI GAATG_Cn'

Cuts at:   3023    3116    3023 
   Size:         93    5419
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2025    2108    2279    2349    2831    3573    3658 
   Size:        399     235      83     171      70     482     742      85

Cuts at:   3658    4729    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     482     399     235     171      85
                  83      70
 
BspEI T'CCGG_A

Cuts at:   5175    5175 
   Size:       5512
 
BspHI T'CATG_A

Cuts at:   3401    5131    3401 
   Size:       1730    3782
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2867     844 
   Size:       2023    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3399    1394 
   Size:        429    1576    3507

  Fragments arranged by size: 

                3507    1576     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2062    4007    4181      65 
   Size:       1183     814    1945     174    1396

  Fragments arranged by size: 

                1945    1396    1183     814     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4156    4343    1700 
   Size:        110    2346     187    2869

  Fragments arranged by size: 

                2869    2346     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2491      96 
   Size:       2395    3117
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5512
 
BssSI C'ACGA_G

Cuts at:   3572    4584    3572 
   Size:       1012    4500
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5512
 
BstBI TT'CG_AA

Cuts at:   4316    4316 
   Size:       5512
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5512
 
BstYI r'GATC_y

Cuts at:   1972    2608    2842    3209    3594    3611    5063    5149    5161 
   Size:        636     234     367     385      17    1452      86      12

Cuts at:   5161    5507    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     636     385     367     346     234      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2214     513 
   Size:        552     939     210    3811

  Fragments arranged by size: 

                3811     939     552     210
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4773

  Fragments arranged by size: 

                4773     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3028    3822    3842    3028 
   Size:        794      20    4698

  Fragments arranged by size: 

                4698     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5512
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5477
 
DraI TTT'AAA

Cuts at:   2816    2816 
   Size:       5512
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4519     809 
   Size:        507    3203    1802

  Fragments arranged by size: 

                3203    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2211    2770    2959 
   Size:         54    1425      50     275      18     381     559     189

Cuts at:   2959    3848    5477       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     559     381     275     189      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2959    1537 
   Size:        275    1147    4090

  Fragments arranged by size: 

                4090    1147     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3442    1353 
   Size:       2089    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4099    4473    4619     387 
   Size:       1064     138    2510     374     146    1280

  Fragments arranged by size: 

                2510    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2023    2023 
   Size:       5512
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2170    2429    2618    3575    4172    4959    5227 
   Size:        393     538     259     189     957     597     787     268

Cuts at:   5227    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     957     787     597     538     393     268     259
                 189
 
EcoO109I rG'GnC_Cy

Cuts at:   2116    2116 
   Size:       5512
 
EcoRI G'AATT_C

Cuts at:   2821    3199    2821 
   Size:        378    5134
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2125    2125 
   Size:       5512
 
FspI TGC'GCA

Cuts at:   4018    4018 
   Size:       5512
 
HaeII r_GCGC'y

Cuts at:   1678    4659    1678 
   Size:       2981    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2401    2433    2593    2625    4153 
   Size:         32     135      32     829      32     160      32    1528

Cuts at:   4153    4185    4227    4259    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1528     829     160     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2856    3102     669 
   Size:        988     281     918     246    3079

  Fragments arranged by size: 

                3079     988     918     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2401     748 
   Size:       1653    3859
 
HpaI GTT'AAC

Cuts at:   3102    3102 
   Size:       5512
 
KpnI G_GTAC'C

Cuts at:   2837    2837 
   Size:       5512
 
MfeI C'AATT_G

Cuts at:   3111    3111 
   Size:       5512
 
MluI A'CGCG_T

Cuts at:   5388    5388 
   Size:       5512
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2187    4626    4810    1305 
   Size:        882    2439     184    2007

  Fragments arranged by size: 

                2439    2007     882     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2213      10 
   Size:         54    1425     343     381    3309

  Fragments arranged by size: 

                3309    1425     381     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3470    3829    3988     518 
   Size:        648    2304     359     159    2042

  Fragments arranged by size: 

                2304    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2596    2928    3605    4753    4998    1606 
   Size:        990     332     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     990     677     332     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2214     513 
   Size:        552     939     210    3811

  Fragments arranged by size: 

                3811     939     552     210
 
NdeI CA'TA_TG

Cuts at:    387    2422     387 
   Size:       2035    3477
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5512
 
NotI GC'GGCC_GC

Cuts at:   2959    2959 
   Size:       5512
 
NspI r_CATG'y

Cuts at:   1132    1229    2743    2870    4415    1132 
   Size:         97    1514     127    1545    2229

  Fragments arranged by size: 

                2229    1545    1514     127      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5512
 
PciI A'CATG_T

Cuts at:   4411    4411 
   Size:       5512
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2119    1108 
   Size:       1011    4501
 
PfoI T'CCnGG_A

Cuts at:   2112    2259    2382    2112 
   Size:        147     123    5242

  Fragments arranged by size: 

                5242     147     123
 
PmeI GTTT'AAAC

Cuts at:   2816    2816 
   Size:       5512
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3581    3613    5094    5126    3581 
   Size:         32    1481      32    3967

  Fragments arranged by size: 

                3967    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2116    2116 
   Size:       5512
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5512
 
PsiI TTA'TAA

Cuts at:   3082    3082 
   Size:       5512
 
PstI C_TGCA'G

Cuts at:    830    2864     830 
   Size:       2034    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5512
 
PvuII CAG'CTG

Cuts at:   2596    2596 
   Size:       5512
 
SacI G_AGCT'C

Cuts at:   2025    2025 
   Size:       5512
 
SalI G'TCGA_C

Cuts at:   1936    2854    1936 
   Size:        918    4594
 
SbfI CC_TGCA'GG

Cuts at:   2864    2864 
   Size:       5512
 
ScaI AGT'ACT

Cuts at:   3760    5472    3760 
   Size:       1712    3800
 
SfcI C'TryA_G

Cuts at:    826    1045    2173    2591    2860    3995    4676    4867     826 
   Size:        219    1128     418     269    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1128     681     418     269     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5512
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5512
 
SmaI CCC'GGG

Cuts at:   1872    2839    1872 
   Size:        967    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2537    2727    3616    4517    4779     820 
   Size:        197     926     594     190     889     901     262    1553

  Fragments arranged by size: 

                1553     926     901     889     594     262     197     190
            
 
SnaBI TAC'GTA

Cuts at:    493    3303     493 
   Size:       2810    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5512
 
SphI G_CATG'C

Cuts at:   1132    2870    1132 
   Size:       1738    3774
 
SspI AAT'ATT

Cuts at:      5      52    3436       5 
   Size:         47    3384    2081

  Fragments arranged by size: 

                3384    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5512
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2214    2529    2951     513 
   Size:        552     939     210     315     422    3074

  Fragments arranged by size: 

                3074     939     552     422     315     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3539    3556    3709    3894    4339     325 
   Size:       1255    1959      17     153     185     445    1498

  Fragments arranged by size: 

                1959    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2491    3758    5470 
   Size:        274      80      33      51    1443     514    1267    1712

Cuts at:   5470      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1267     514     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2241    2269    2273    2301    5472    5504    2241 
   Size:         28       4      28    3171      32    2249

  Fragments arranged by size: 

                3171    2249      32      28      28       4
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5512
 
XbaI T'CTAG_A

Cuts at:   2848    2848 
   Size:       5512
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2035    2593    2035 
   Size:        558    4954
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5512
 
XmaI C'CCGG_G

Cuts at:   1870    2837    1870 
   Size:        967    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3641    3641 
   Size:       5512
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3320     276 
   Size:         53      83     186     936    1786    2468

  Fragments arranged by size: 

                2468    1786     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com