Restriction map for
pFN21AB6377
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlpI BmyI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HpaI HpaII HphI KasI KpnI
KspI MaeII MamI MflI MluI
MroI MslI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 412 GACGCC G[GA]^CG[CT]C
AfaI 450 GTAC GT^AC
AflIII 17 ACATGT A^C[GA][CT]GT
AlwNI 793 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 331 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BfaI 332 CTAG C^TAG
BlnI 331 CCTAGG C^CTAGG
BpmI 289 CTGGAG CTGGAG(16/14)
BsaHI 412 GACGCC G[GA]^CG[CT]C
BsaI 652 GAGACC GGTCTC(1/5)
BsaJI 331 CCTAGG C^C[ACGT][ACGT]GG
BseRI 661 CTCCTC GAGGAG(10/8)
BsmBI 249 GAGACG CGTCTC(1/5)
BspLU11I 17 ACATGT A^CATGT
BsrDI 359 GCAATG GCAATG(2/0)
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoRI 397 GAATTC G^AATTC
EcoT14I 331 CCTAGG C^C[AT][AT]GG
HgaI 412 GACGC GACGC(5/10)
Hin1I 412 GACGCC G[GA]^CG[CT]C
HindIII 190 AAGCTT A^AGCTT
MaeI 332 CTAG C^TAG
MfeI 568 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 262 TTAA T^TAA
MunI 568 CAATTG C^AATTG
MwoI 350 GCTTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 768 GGGGCC GG[ACGT]^[ACGT]CC
NspI 17 ACATGT [GA]CATG^[CT]
PleI 307 GACTC GAGTC(4/5)
RsaI 450 GTAC GT^AC
StyI 331 CCTAGG C^C[AT][AT]GG
TaqI 435 TCGA T^CGA
Tru9I 262 TTAA T^TAA
Tsp45I 577 GTGAC ^GT[GC]AC
TthHB8I 435 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 397 GAATTC [GA]^AATT[CT]
493 AAATTT
AlwI 144 GGATC GGATC(4/5)
383 GGATC
ApoI 397 GAATTC [GA]^AATT[CT]
493 AAATTT
Asp700I 96 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
693 GAACTATTTC
BclI 75 TGATCA T^GATCA
444 TGATCA
BssKI 288 CCTGG ^CC[ACGT]GG
807 CCAGG
BstNI 288 CCTGG CC^[AT]GG
807 CCAGG
Cfr13I 121 GGCCC G^G[ACGT]CC
769 GGGCC
EarI 664 CTCTTC CTCTTC(1/4)
246 GAAGAG
EcoRII 288 CCTGG ^CC[AT]GG
807 CCAGG
FbaI 75 TGATCA T^GATCA
444 TGATCA
Ksp632I 664 CTCTTC CTCTTC(1/4)
246 GAAGAG
MaeIII 41 GTTAC ^GT[ACGT]AC
577 GTGAC
MvaI 288 CCTGG CC^[AT]GG
807 CCAGG
Sau96I 121 GGCCC G^G[ACGT]CC
769 GGGCC
ScrFI 288 CCTGG CC^[ACGT]GG
807 CCAGG
SfaNI 529 GATGC GCATC(5/9)
618 GATGC
XmnI 96 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
693 GAACTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BbsI BbvI BpuAI BsmAI
BsrI BstF5I DdeI DpnII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MboII MnlI NdeII NlaIII Sau3AI
TfiI TseI Tsp509I TspRI