(Circular) (Six-base) MAPSORT of: pFN21AB6377.seq Check: 4171 from: 1 to: 5518 pFN21AB6377 5518 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2267 2636 3581 4965 5233 1632 Size: 425 210 369 945 1384 268 1917 Fragments arranged by size: 1917 1384 945 425 369 268 210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 2022 4417 5394 1203 Size: 819 2395 977 1327 Fragments arranged by size: 2395 1327 977 819 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 2803 3261 4833 2803 Size: 458 1572 3488 Fragments arranged by size: 3488 1572 458 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2402 2498 2827 3053 3205 5314 1493 Size: 909 96 329 226 152 2109 1697 Fragments arranged by size: 2109 1697 909 329 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2843 1870 Size: 73 900 4545 Fragments arranged by size: 4545 900 73 AvrII C'CTAG_G Cuts at: 2336 2336 Size: 5518 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 4294 618 Size: 325 217 627 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 217 BanII G_rGCy'C Cuts at: 1391 2837 1391 Size: 1446 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2070 2604 928 Size: 971 138 33 534 3842 Fragments arranged by size: 3842 971 534 138 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 1903 Size: 34 1772 34 3678 Fragments arranged by size: 3678 1772 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 2080 2449 1548 Size: 532 369 4617 Fragments arranged by size: 4617 532 369 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 3579 4735 1790 Size: 1789 1156 2573 Fragments arranged by size: 2573 1789 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2764 3249 4207 Size: 416 53 83 399 24 1347 485 958 Cuts at: 4207 442 Size: 1753 Fragments arranged by size: 1753 1347 958 485 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 4178 1239 Size: 1076 1863 2579 Fragments arranged by size: 2579 1863 1076 Bpu10I CC'TnA_GC Cuts at: 2360 2360 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2651 4181 882 Size: 617 1152 1530 2219 Fragments arranged by size: 2219 1530 1152 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2418 3325 3707 4342 Size: 53 83 186 936 885 907 382 635 Cuts at: 4342 275 Size: 1451 Fragments arranged by size: 1451 936 907 885 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3951 4349 4623 4770 5181 3951 Size: 398 274 147 411 4288 Fragments arranged by size: 4288 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 1178 Size: 30 165 30 654 30 4609 Fragments arranged by size: 4609 654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2657 1186 Size: 1471 4047 BseYI C'CCAG_C Cuts at: 4721 5217 4721 Size: 496 5022 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4945 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3579 3664 4735 3579 Size: 85 1071 4362 Fragments arranged by size: 4362 1071 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 BsmBI CGTCTCn'nnnn_ Cuts at: 2248 2248 Size: 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2837 3579 3664 4735 1391 Size: 399 1047 742 85 1071 2174 Fragments arranged by size: 2174 1071 1047 742 399 85 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2873 844 Size: 2029 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2371 4013 4187 65 Size: 1183 1123 1642 174 1396 Fragments arranged by size: 1642 1396 1183 1123 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4162 4349 1700 Size: 110 2352 187 2869 Fragments arranged by size: 2869 2352 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513 Size: 876 367 385 17 1452 86 12 346 Cuts at: 5513 1972 Size: 1977 Fragments arranged by size: 1977 1452 876 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2287 487 Size: 683 56 1061 3718 Fragments arranged by size: 3718 1061 683 56 BtsI GCAGTG_nn' Cuts at: 2499 3034 3828 3848 2499 Size: 535 794 20 4169 Fragments arranged by size: 4169 794 535 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2965 3854 Size: 54 1425 50 275 18 177 958 889 Cuts at: 3854 5483 8 Size: 1629 43 Fragments arranged by size: 1629 1425 958 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 2675 3448 1353 Size: 893 429 773 3423 Fragments arranged by size: 3423 893 773 429 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2267 2315 2636 3581 4178 4965 Size: 393 425 210 48 321 945 597 787 Cuts at: 4965 5233 1239 Size: 268 1524 Fragments arranged by size: 1524 945 787 597 425 393 321 268 210 48 EcoRI G'AATT_C Cuts at: 2402 2827 3205 2402 Size: 425 378 4715 Fragments arranged by size: 4715 425 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 5518 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2072 2089 2104 4159 Size: 32 135 32 485 15 17 15 2055 Cuts at: 4159 4191 4233 4265 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2055 485 135 42 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 2195 748 Size: 1447 4071 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 2573 3117 2573 Size: 544 4974 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3519 Fragments arranged by size: 3519 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2934 3611 4759 5004 1606 Size: 1328 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2026 2876 4421 1132 Size: 97 797 850 1545 2229 Fragments arranged by size: 2229 1545 850 797 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 2022 4417 2022 Size: 2395 3123 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 2517 3088 2517 Size: 571 4947 PstI C_TGCA'G Cuts at: 830 2870 830 Size: 2040 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2866 4001 4682 4873 826 Size: 219 1821 1135 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2004 2336 2957 513 Size: 552 939 332 621 3074 Fragments arranged by size: 3074 939 621 552 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 2746 3545 3562 3715 3900 4345 Size: 1255 918 248 799 17 153 185 445 Cuts at: 4345 325 Size: 1498 Fragments arranged by size: 1498 1255 918 799 445 248 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 2105 2702 3647 2105 Size: 597 945 3976 Fragments arranged by size: 3976 945 597 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI