(Linear) (Six-base) MAPSORT of: KIBB6377.seq  Check: 3970  from: 1  to: 813



KIBB6377.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     262     631     813 
   Size:         52     210     369     182

  Fragments arranged by size: 

                 369     210     182      52
 
AflIII A'CryG_T

Cuts at:      0      17     813 
   Size:         17     796
 
AlwNI CAG_nnn'CTG

Cuts at:      0     798     813 
   Size:        798      15
 
ApoI r'AATT_y

Cuts at:      0     397     493     813 
   Size:        397      96     320

  Fragments arranged by size: 

                 397     320      96
 
AvrII C'CTAG_G

Cuts at:      0     331     813 
   Size:        331     482
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32      65     599     813 
   Size:         32      33     534     214

  Fragments arranged by size: 

                 534     214      33      32
 
BclI T'GATC_A

Cuts at:      0      75     444     813 
   Size:         75     369     369

  Fragments arranged by size: 

                 369     369      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     759     813 
   Size:        759      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     813 
   Size:        310     503
 
Bpu10I CC'TnA_GC

Cuts at:      0     355     813 
   Size:        355     458
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     646     813 
   Size:        646     167
 
BsaHI Gr'CG_yC

Cuts at:      0     413     813 
   Size:        413     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      52      82     813 
   Size:         52      30     731

  Fragments arranged by size: 

                 731      52      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     652     813 
   Size:        652     161
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     243     813 
   Size:        243     570
 
BsrDI GCAATG_nn'

Cuts at:      0     366     813 
   Size:        366     447
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     282     813 
   Size:        282     531
 
BtsI GCAGTG_nn'

Cuts at:      0     494     813 
   Size:        494     319
 
EaeI y'GGCC_r

Cuts at:      0       2     813 
   Size:          2     811
 
EarI CTCTTCn'nnn_

Cuts at:      0     241     670     813 
   Size:        241     429     143

  Fragments arranged by size: 

                 429     241     143
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     262     310     631     813 
   Size:         52     210      48     321     182

  Fragments arranged by size: 

                 321     210     182      52      48
 
EcoRI G'AATT_C

Cuts at:      0     397     813 
   Size:        397     416
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     213     813 
   Size:        213     600
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      52      67      84      99     813 
   Size:         52      15      17      15     714

  Fragments arranged by size: 

                 714      52      17      15      15
 
HindIII A'AGCT_T

Cuts at:      0     190     813 
   Size:        190     623
 
MfeI C'AATT_G

Cuts at:      0     568     813 
   Size:        568     245
 
MscI TGG'CCA

Cuts at:      0       4     813 
   Size:          4     809
 
NspI r_CATG'y

Cuts at:      0      21     813 
   Size:         21     792
 
PciI A'CATG_T

Cuts at:      0      17     813 
   Size:         17     796
 
PsiI TTA'TAA

Cuts at:      0     512     813 
   Size:        512     301
 
StyI C'CwwG_G

Cuts at:      0     331     813 
   Size:        331     482
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     493     741     813 
   Size:        493     248      72

  Fragments arranged by size: 

                 493     248      72
 
XmnI GAAnn'nnTTC

Cuts at:      0     100     697     813 
   Size:        100     597     116

  Fragments arranged by size: 

                 597     116     100
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    AvrII     BbsI     BclI     BmrI
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BsmBI    BsrDI
    BtgZI     BtsI     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I
  HindIII     MfeI     MscI     NspI     PciI     PsiI     StyI    TaqII
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI