Restriction map for
pFN21AB3719
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# NO CUT ENZYMES ( 81 enzymes) #
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AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MunI NdeI NotI
NruI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SalI SbfI ScaI SexAI SgrAI
SpeI Sse8387I SspBI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1534 GACGTC GACGT^C
Acc65I 1565 GGTACC G^GTACC
AccI 2306 GTAGAC GT^[AC][GT]AC
AccIII 939 TCCGGA T^CCGGA
AclI 368 AACGTT AA^CGTT
AocI 2505 CCTGAGG CC^T[ACGT]AGG
Asp700I 931 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1565 GGTACC G^GTACC
AspI 1177 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1898 TGGCCA TGG^CCA
BfaI 1039 CTAG C^TAG
BsaWI 939 TCCGGA [AT]^CCGG[AT]
BseAI 939 TCCGGA T^CCGGA
BsmBI 2204 CGTCTC CGTCTC(1/5)
BspEI 939 TCCGGA T^CCGGA
BspHI 1274 TCATGA T^CATGA
BsrBI 1570 CCGCTC CCGCTC(-3/-3)
BssHII 1353 GCGCGC G^CGCGC
BstAPI 2361 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1753 TTCGAA TT^CGAA
BstXI 986 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2505 CCTGAGG CC^T[ACGT]AGG
Eco81I 2505 CCTGAGG CC^T[ACGT]AGG
EcoNI 2217 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 585 ATGCAT ATGCA^T
HincII 451 GTTGAC GT[CT]^[GA]AC
HindII 451 GTTGAC GT[CT]^[GA]AC
KpnI 1565 GGTACC GGTAC^C
MaeI 1039 CTAG C^TAG
MluNI 1898 TGGCCA TGG^CCA
MroI 939 TCCGGA T^CCGGA
MscI 1898 TGGCCA TGG^CCA
MseI 818 TTAA T^TAA
NaeI 2469 GCCGGC GCC^GGC
NgoMIV 2469 GCCGGC G^CCGGC
NheI 1038 GCTAGC G^CTAGC
NsiI 585 ATGCAT ATGCA^T
NspV 1753 TTCGAA TT^CGAA
PaeR7I 325 CTCGAG C^TCGAG
PflFI 1177 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 368 AACGTT AA^CGTT
RcaI 1274 TCATGA T^CATGA
SfuI 1753 TTCGAA TT^CGAA
SmlI 325 CTCGAG C^T[CT][GA]AG
SnaBI 1495 TACGTA TAC^GTA
SphI 1637 GCATGC GCATG^C
Tru9I 818 TTAA T^TAA
Tth111I 1177 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 325 CTCGAG C^TCGAG
XmnI 931 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 534 GAATTT [GA]^AATT[CT]
1189 AAATTC
ApoI 534 GAATTT [GA]^AATT[CT]
1189 AAATTC
BsaAI 1171 TACGTG [CT]AC^GT[GA]
1495 TACGTA
BsaI 263 GAGACC GGTCTC(1/5)
628 GAGACC
BsrFI 1843 ACCGGC [GA]^CCGG[CT]
2469 GCCGGC
BstEII 474 GGTGACC G^GT[ACGT]ACC
573 GGTAACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
573 GGTAACC
Cfr10I 1843 ACCGGC [GA]^CCGG[CT]
2469 GCCGGC
EaeI 1898 TGGCCA [CT]^GGCC[GA]
2033 TGGCCG
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
573 GGTAACC
NcoI 2198 CCATGG C^CATGG
2315 CCATGG
SacI 591 GAGCTC GAGCT^C
2397 GAGCTC
SapI 1246 GAAGAGC GCTCTTC(1/4)
2262 GCTCTTC
SfiI 1890 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2025 GGCCCTGGTGGCC
StuI 1889 AGGCCT AGG^CCT
2102 AGGCCT
TfiI 186 GATTC G^A[AT]TC
676 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI