Restriction map for
pFN21AB3719
(Circular) (Six-base) MAPSORT of: pFN21AB3719.seq Check: 4403 from: 1 to: 7303
pFN21AB3719 7303 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2502 3972 2502
Size: 1470 5833
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3543 5113 278
Size: 53 83 186 936 2007 1570 2468
Fragments arranged by size:
2468 2007 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4312 4646 1937
Size: 2375 334 4594
Fragments arranged by size:
4594 2375 334
Acc65I G'GTAC_C
Cuts at: 3570 4624 3570
Size: 1054 6249
AclI AA'CG_TT
Cuts at: 1563 2374 5430 5803 1563
Size: 811 3056 373 3063
Fragments arranged by size:
3063 3056 811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2848 3149 3499 5366 6750 7018 1632
Size: 793 423 301 350 1867 1384 268 1917
Fragments arranged by size:
1917 1867 1384 793 423 350 301 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 6202 7179 1203
Size: 4999 977 1327
Fragments arranged by size:
4999 1327 977
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 3230 4007 1166
Size: 2064 777 4462
Fragments arranged by size:
4462 2064 777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7303
AlwNI CAG_nnn'CTG
Cuts at: 2701 2871 3208 3619 4282 5046 6618 2701
Size: 170 337 411 663 764 1572 3386
Fragments arranged by size:
3386 1572 764 663 411 337 170
ApaI G_GGCC'C
Cuts at: 2046 4033 4059 4596 2046
Size: 1987 26 537 4753
Fragments arranged by size:
4753 1987 537 26
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 2539 3194 4612 4838 4990 7099 1493
Size: 1046 655 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 1046 655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5857 160
Size: 872 4825 1606
Fragments arranged by size:
4825 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2086 2330 3688 3972 4129 4172
Size: 73 95 48 244 1358 284 157 43
Cuts at: 4172 4418 4493 4558 4628 1870
Size: 246 75 65 70 4545
Fragments arranged by size:
4545 1358 284 246 244 157 95 75
73 70 65 48 43
BamHI G'GATC_C
Cuts at: 4633 5000 4633
Size: 367 6936
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 3391 3494 3562 3570
Size: 325 217 627 306 1298 103 68 8
Cuts at: 3570 3865 4070 4624 6079 618
Size: 295 205 554 1455 1842
Fragments arranged by size:
1842 1455 1298 627 554 325 306 295
217 205 103 68 8
BanII G_rGCy'C
Cuts at: 1391 2046 2600 3008 3689 4033 4059 4406 4596
Size: 655 554 408 681 344 26 347 190
Cuts at: 4596 4622 1391
Size: 26 4072
Fragments arranged by size:
4072 681 655 554 408 347 344 190
26 26
BbeI G_GCGC'C
Cuts at: 2097 3395 3566 3869 2097
Size: 1298 171 303 5531
Fragments arranged by size:
5531 1298 303 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5494 5528 1903
Size: 34 3557 34 3678
Fragments arranged by size:
3678 3557 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7303
BfrBI ATG'CAT
Cuts at: 2592 2592
Size: 7303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3400 3693
Size: 107 122 71 631 605 337 1391 293
Cuts at: 3693 3902 4037 5914 136
Size: 209 135 1877 1525
Fragments arranged by size:
1877 1525 1391 631 605 337 293 209
135 122 107 71
BglII A'GATC_T
Cuts at: 7298 7298
Size: 7303
BlpI GC'TnA_GC
Cuts at: 4720 4720
Size: 7303
Bme1580I G_kGCm'C
Cuts at: 1790 2046 4033 4059 4596 5364 6520 1790
Size: 256 1987 26 537 768 1156 2573
Fragments arranged by size:
2573 1987 1156 768 537 256 26
BmgBI CAC'GTC
Cuts at: 1318 3184 4234 1318
Size: 1866 1050 4387
Fragments arranged by size:
4387 1866 1050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3196 4590 5034
Size: 416 53 83 399 24 1779 1394 444
Cuts at: 5034 5992 442
Size: 958 1753
Fragments arranged by size:
1779 1753 1394 958 444 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3047 1056
Size: 1991 5312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3340 3904 3340
Size: 564 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 2916 3058 3641 4381 4505 4523 5963
Size: 1421 256 142 583 740 124 18 1440
Cuts at: 5963 1239
Size: 2579
Fragments arranged by size:
2579 1440 1421 740 583 256 142 124
18
Bpu10I CC'TnA_GC
Cuts at: 2599 2599
Size: 7303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5428 6293 6591 5428
Size: 865 298 6140
Fragments arranged by size:
6140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2262 2627 5966 882
Size: 617 763 365 3339 2219
Fragments arranged by size:
3339 2219 763 617 365
BsaAI yAC'GTr
Cuts at: 493 1204 3178 3502 5094 493
Size: 711 1974 324 1592 2702
Fragments arranged by size:
2702 1974 1592 711 324
BsaBI GATnn'nnATC
Cuts at: 7297 7297
Size: 7303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2313 3392 3540
Size: 53 83 186 936 561 219 1079 148
Cuts at: 3540 3563 3866 4208 5110 5492 6127 275
Size: 23 303 342 902 382 635 1451
Fragments arranged by size:
1451 1079 936 902 635 561 382 342
303 219 186 148 83 53 23
BsaWI w'CCGG_w
Cuts at: 2944 5736 6134 6408 6555 6966 2944
Size: 2792 398 274 147 411 3281
Fragments arranged by size:
3281 2792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3927 3957 1178
Size: 30 165 30 2524 30 4524
Fragments arranged by size:
4524 2524 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2206 3608 3839 3948 4037 4367 4370 4460
Size: 1020 1402 231 109 89 330 3 90
Cuts at: 4460 4469 4528 1186
Size: 9 59 3961
Fragments arranged by size:
3961 1402 1020 330 231 109 90 89
59 9 3
BseYI C'CCAG_C
Cuts at: 2050 2136 3047 4120 4136 4563 6506 7002 2050
Size: 86 911 1073 16 427 1943 496 2351
Fragments arranged by size:
2351 1943 1073 911 496 427 86 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2228 2358 2867 4394 4620 1163
Size: 573 401 91 130 509 1527 226 3846
Fragments arranged by size:
3846 1527 573 509 401 226 130 91
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4753 5514 6542 1540
Size: 275 186 2752 761 1028 2301
Fragments arranged by size:
2752 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2565 2600 2748 3347 4014 4245 4406 5364 5449
Size: 35 148 599 667 231 161 958 85
Cuts at: 5449 6520 2565
Size: 1071 3348
Fragments arranged by size:
3348 1071 958 667 599 231 161 148
85 35
BsmI GAATG_Cn'
Cuts at: 4814 4907 4814
Size: 93 7210
BsmBI CGTCTCn'nnnn_
Cuts at: 4215 4215
Size: 7303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2565 2600 2748 3008 3347 3689
Size: 399 256 519 35 148 260 339 342
Cuts at: 3689 4014 4033 4059 4245 4406 4596 4622 5364
Size: 325 19 26 186 161 190 26 742
Cuts at: 5364 5449 6520 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 519 399 342 339 325
260 256 190 186 161 148 85 35
26 26 19
BspEI T'CCGG_A
Cuts at: 2944 6966 2944
Size: 4022 3281
BspHI T'CATG_A
Cuts at: 3279 5192 6922 3279
Size: 1913 1730 3660
Fragments arranged by size:
3660 1913 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 2238 2502 2770 3252 3687 3727 3972
Size: 1336 58 264 268 482 435 40 245
Cuts at: 3972 4658 844
Size: 686 3489
Fragments arranged by size:
3489 1336 686 482 435 268 264 245
58 40
BsrBI CCG'CTC
Cuts at: 1394 1823 3577 5190 1394
Size: 429 1754 1613 3507
Fragments arranged by size:
3507 1754 1613 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5798 5972 65
Size: 1183 4550 174 1396
Fragments arranged by size:
4550 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3848 4474 5947 6134 1700
Size: 110 2038 626 1473 187 2869
Fragments arranged by size:
2869 2038 1473 626 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7303
BssHII G'CGCG_C
Cuts at: 1925 3358 1925
Size: 1433 5870
BssSI C'ACGA_G
Cuts at: 5363 6375 5363
Size: 1012 6291
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4372 794
Size: 3578 3725
BstBI TT'CG_AA
Cuts at: 3759 6107 3759
Size: 2348 4955
BstEII G'GTnAC_C
Cuts at: 1184 2479 2578 1184
Size: 1295 99 5909
Fragments arranged by size:
5909 1295 99
BstXI CCAn_nnnn'nTGG
Cuts at: 2998 2998
Size: 7303
BstYI r'GATC_y
Cuts at: 1972 2235 2697 2850 4633 5000 5385 5402 6854
Size: 263 462 153 1783 367 385 17 1452
Cuts at: 6854 6940 6952 7298 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1783 1452 462 385 367 346 263
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4511 4511
Size: 7303
BtgI C'CryG_G
Cuts at: 513 1065 2004 2280 2628 3349 3415 3426 3566
Size: 552 939 276 348 721 66 11 140
Cuts at: 3566 4041 4164 4203 4320 513
Size: 475 123 39 117 3496
Fragments arranged by size:
3496 939 721 552 475 348 276 140
123 117 66 39 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6564
Fragments arranged by size:
6564 683 56
BtsI GCAGTG_nn'
Cuts at: 2300 3441 3830 3948 4819 5613 5633 2300
Size: 1141 389 118 871 794 20 3970
Fragments arranged by size:
3970 1141 871 794 389 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7268
DraI TTT'AAA
Cuts at: 4607 4607
Size: 7303
DraIII CAC_nnn'GTG
Cuts at: 2452 2673 2845 2452
Size: 221 172 6910
Fragments arranged by size:
6910 221 172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3539 3737 3917 6310 809
Size: 507 2223 198 180 2393 1802
Fragments arranged by size:
2393 2223 1802 507 198 180
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3903 4038 4750
Size: 54 1425 50 275 18 2073 135 712
Cuts at: 4750 5639 7268 8
Size: 889 1629 43
Fragments arranged by size:
2073 1629 1425 889 712 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4750 1537
Size: 275 2938 4090
Fragments arranged by size:
4090 2938 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2772 3246 3807 4160 4274 4437 4446 5233
Size: 1419 474 561 353 114 163 9 787
Cuts at: 5233 1353
Size: 3423
Fragments arranged by size:
3423 1419 787 561 474 353 163 114
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4529 5890 6264 6410 387
Size: 1064 138 2940 1361 374 146 1280
Fragments arranged by size:
2940 1361 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2598 4404 2598
Size: 1806 5497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2660 2848 2916 3058 3149 3499
Size: 393 793 235 188 68 142 91 350
Cuts at: 3499 3641 4381 4505 4523 5366 5963 6750 7018
Size: 142 740 124 18 843 597 787 268
Cuts at: 7018 1239
Size: 1524
Fragments arranged by size:
1524 843 793 787 740 597 393 350
268 235 188 142 142 124 91 68
18
EcoNI CCTnn'n_nnAGG
Cuts at: 4226 4226
Size: 7303
EcoO109I rG'GnC_Cy
Cuts at: 2042 2043 2227 2518 2614 3433 3616 4029 4030
Size: 1 184 291 96 819 183 413 1
Cuts at: 4030 4056 4462 4498 4592 2042
Size: 26 406 36 94 4753
Fragments arranged by size:
4753 819 413 406 291 184 183 96
94 36 26 1 1
EcoRI G'AATT_C
Cuts at: 4612 4990 4612
Size: 378 6925
FspI TGC'GCA
Cuts at: 5809 5809
Size: 7303
HaeII r_GCGC'y
Cuts at: 1678 2097 2126 3377 3395 3566 3869 3989 4137
Size: 419 29 1251 18 171 303 120 148
Cuts at: 4137 6450 1678
Size: 2313 2531
Fragments arranged by size:
2531 2313 1251 419 303 171 148 120
29 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2182 2214 2384 2416 2784
Size: 32 135 32 610 32 170 32 368
Cuts at: 2784 2816 3785 3817 5944 5976 6018 6050 1373
Size: 32 969 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 969 610 368 170 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 4647 4893 669
Size: 988 281 520 2189 246 3079
Fragments arranged by size:
3079 2189 988 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7303
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KasI G'GCGC_C
Cuts at: 2093 3391 3562 3865 2093
Size: 1298 171 303 5531
Fragments arranged by size:
5531 1298 303 171
KpnI G_GTAC'C
Cuts at: 3574 4628 3574
Size: 1054 6249
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 6417 6601 1305
Size: 1515 3597 184 2007
Fragments arranged by size:
3597 2007 1515 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3905 10
Size: 54 1425 343 2073 3408
Fragments arranged by size:
3408 2073 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3230 3455 4007 4202 4433 5261
Size: 648 1461 603 225 552 195 231 828
Cuts at: 5261 5620 5779 518
Size: 359 159 2042
Fragments arranged by size:
2042 1461 828 648 603 552 359 231
225 195 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2187 2242 2359 2653 2871 3127 3351
Size: 427 154 55 117 294 218 256 224
Cuts at: 3351 3528 3568 3744 3916 4043 4117 4363 4525
Size: 177 40 176 172 127 74 246 162
Cuts at: 4525 4531 4546 4719 5396 6544 6789 1606
Size: 6 15 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 427 294 256 246 245
224 218 177 176 173 172 162 154
127 117 74 55 40 15 6
NaeI GCC'GGC
Cuts at: 4476 4476
Size: 7303
NarI GG'CG_CC
Cuts at: 2094 3392 3563 3866 2094
Size: 1298 171 303 5531
Fragments arranged by size:
5531 1298 303 171
NcoI C'CATG_G
Cuts at: 513 1065 2004 4203 4320 513
Size: 552 939 2199 117 3496
Fragments arranged by size:
3496 2199 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7303
NgoMIV G'CCGG_C
Cuts at: 4474 4474
Size: 7303
NheI G'CTAG_C
Cuts at: 1052 3043 1052
Size: 1991 5312
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NsiI A_TGCA'T
Cuts at: 2594 2594
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 2752 2956 3646 4661 6206 1132
Size: 97 1523 204 690 1015 1545 2229
Fragments arranged by size:
2229 1545 1523 1015 690 204 97
PasI CC'CwG_GG
Cuts at: 1348 4297 4466 1348
Size: 2949 169 4185
Fragments arranged by size:
4185 2949 169
PciI A'CATG_T
Cuts at: 6202 6202
Size: 7303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 3619 4006 1108
Size: 1344 1167 387 4405
Fragments arranged by size:
4405 1344 1167 387
PfoI T'CCnGG_A
Cuts at: 2109 3465 3612 3726 3841 4397 2109
Size: 1356 147 114 115 556 5015
Fragments arranged by size:
5015 1356 556 147 115 114
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5372 5404 6885 6917 3761
Size: 32 1579 32 1481 32 4147
Fragments arranged by size:
4147 1579 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2518 2614 3433 3616 4462 4498 2227
Size: 291 96 819 183 846 36 5032
Fragments arranged by size:
5032 846 819 291 183 96 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7303
PsiI TTA'TAA
Cuts at: 4873 4873
Size: 7303
PspOMI G'GGCC_C
Cuts at: 2042 4029 4055 4592 2042
Size: 1987 26 537 4753
Fragments arranged by size:
4753 1987 537 26
PspXI vC'TCGA_Gb
Cuts at: 2330 2330
Size: 7303
PstI C_TGCA'G
Cuts at: 830 2235 2307 2358 3448 3969 4310 4551 4655
Size: 1405 72 51 1090 521 341 241 104
Cuts at: 4655 830
Size: 3478
Fragments arranged by size:
3478 1405 1090 521 341 241 104 72
51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 2187 2242 2359 2653 2871 3528 4531 4546 2187
Size: 55 117 294 218 657 1003 15 4944
Fragments arranged by size:
4944 1003 657 294 218 117 55 15
SacI G_AGCT'C
Cuts at: 2600 4406 2600
Size: 1806 5497
SacII CC_GC'GG
Cuts at: 3352 3569 4044 3352
Size: 217 475 6611
Fragments arranged by size:
6611 475 217
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SanDI GG'GwC_CC
Cuts at: 2518 2614 2518
Size: 96 7207
SapI GCTCTTCn'nnn_
Cuts at: 3246 4274 3246
Size: 1028 6275
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 5551 7263 5551
Size: 1712 5591
SfcI C'TryA_G
Cuts at: 826 1045 2231 2303 2354 3444 3965 4306 4547
Size: 219 1186 72 51 1090 521 341 241
Cuts at: 4547 4651 5786 6467 6658 826
Size: 104 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 1090 681 521 341 241
219 191 104 72 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 3902 4037 3902
Size: 135 7168
SfoI GGC'GCC
Cuts at: 2095 3393 3564 3867 2095
Size: 1298 171 303 5531
Fragments arranged by size:
5531 1298 303 171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 2040 2088 3690 3974 4131 4630 1872
Size: 168 48 1602 284 157 499 4545
Fragments arranged by size:
4545 1602 499 284 168 157 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 5407 6308 6570 820
Size: 197 926 387 3077 901 262 1553
Fragments arranged by size:
3077 1553 926 901 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3502 5094 493
Size: 3009 1592 2702
Fragments arranged by size:
3009 2702 1592
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7303
SphI G_CATG'C
Cuts at: 1132 3646 4661 1132
Size: 2514 1015 3774
Fragments arranged by size:
3774 2514 1015
SrfI GCCC'GGGC
Cuts at: 3690 4131 3690
Size: 441 6862
SspI AAT'ATT
Cuts at: 5 52 5227 5
Size: 47 5175 2081
Fragments arranged by size:
5175 2081 47
StuI AGG'CCT
Cuts at: 1517 3896 4109 1517
Size: 2379 213 4711
Fragments arranged by size:
4711 2379 213
StyI C'CwwG_G
Cuts at: 513 1065 2004 4186 4203 4320 4742 513
Size: 552 939 2182 17 117 422 3074
Fragments arranged by size:
3074 2182 939 552 422 117 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 2953 3277 5330 5347 5500 5685
Size: 1255 693 680 324 2053 17 153 185
Cuts at: 5685 6130 325
Size: 445 1498
Fragments arranged by size:
2053 1498 1255 693 680 445 324 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 3600 5549
Size: 274 80 33 51 1443 990 633 1949
Cuts at: 5549 7261 96
Size: 1712 138
Fragments arranged by size:
1949 1712 1443 990 633 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2809 3411 3443 3927 3959 7263 7295 2777
Size: 32 602 32 484 32 3304 32 2785
Fragments arranged by size:
3304 2785 602 484 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3185 1316
Size: 1869 5434
XbaI T'CTAG_A
Cuts at: 4639 4639
Size: 7303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 2998 3913 2626
Size: 372 915 6016
Fragments arranged by size:
6016 915 372
XhoI C'TCGA_G
Cuts at: 1943 2330 1943
Size: 387 6916
XmaI C'CCGG_G
Cuts at: 1870 2038 2086 3688 3972 4129 4628 1870
Size: 168 48 1602 284 157 499 4545
Fragments arranged by size:
4545 1602 499 284 168 157 48
XmnI GAAnn'nnTTC
Cuts at: 2940 5432 2940
Size: 2492 4811
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3541 5111 276
Size: 53 83 186 936 2007 1570 2468
Fragments arranged by size:
2468 2007 1570 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI PacI PmlI PsrI
RsrII SexAI SwaI