Restriction map for
pFN21AB3719
(Linear) (Six-base) MAPSORT of: KIBB3719.seq Check: 7075 from: 1 to: 2598
KIBB3719.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 497 1967 2598
Size: 497 1470 631
Fragments arranged by size:
1470 631 497
AatII G_ACGT'C
Cuts at: 0 1538 2598
Size: 1538 1060
AccI GT'mk_AC
Cuts at: 0 2307 2598
Size: 2307 291
Acc65I G'GTAC_C
Cuts at: 0 1565 2598
Size: 1565 1033
AclI AA'CG_TT
Cuts at: 0 369 2598
Size: 369 2229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 843 1144 1494 2598
Size: 420 423 301 350 1104
Fragments arranged by size:
1104 423 420 350 301
AleI CACnn'nnGTG
Cuts at: 0 1225 2002 2598
Size: 1225 777 596
Fragments arranged by size:
1225 777 596
AlwNI CAG_nnn'CTG
Cuts at: 0 696 866 1203 1614 2277 2598
Size: 696 170 337 411 663 321
Fragments arranged by size:
696 663 411 337 321 170
ApaI G_GGCC'C
Cuts at: 0 41 2028 2054 2591 2598
Size: 41 1987 26 537 7
Fragments arranged by size:
1987 537 41 26 7
ApoI r'AATT_y
Cuts at: 0 534 1189 2598
Size: 534 655 1409
Fragments arranged by size:
1409 655 534
AvaI C'yCGr_G
Cuts at: 0 33 81 325 1683 1967 2124 2167 2413
Size: 33 48 244 1358 284 157 43 246
Cuts at: 2413 2488 2553 2598
Size: 75 65 45
Fragments arranged by size:
1358 284 246 244 157 75 65 48
45 43 33
BanI G'GyrC_C
Cuts at: 0 88 1386 1489 1557 1565 1860 2065 2598
Size: 88 1298 103 68 8 295 205 533
Fragments arranged by size:
1298 533 295 205 103 88 68 8
BanII G_rGCy'C
Cuts at: 0 41 595 1003 1684 2028 2054 2401 2591
Size: 41 554 408 681 344 26 347 190
Cuts at: 2591 2598
Size: 7
Fragments arranged by size:
681 554 408 347 344 190 41 26
7
BbeI G_GCGC'C
Cuts at: 0 92 1390 1561 1864 2598
Size: 92 1298 171 303 734
Fragments arranged by size:
1298 734 303 171 92
BfrBI ATG'CAT
Cuts at: 0 587 2598
Size: 587 2011
BglI GCCn_nnn'nGGC
Cuts at: 0 1395 1688 1897 2032 2598
Size: 1395 293 209 135 566
Fragments arranged by size:
1395 566 293 209 135
Bme1580I G_kGCm'C
Cuts at: 0 41 2028 2054 2591 2598
Size: 41 1987 26 537 7
Fragments arranged by size:
1987 537 41 26 7
BmgBI CAC'GTC
Cuts at: 0 1179 2229 2598
Size: 1179 1050 369
Fragments arranged by size:
1179 1050 369
BmrI ACTGGGnnnn_n'
Cuts at: 0 1191 2585 2598
Size: 1191 1394 13
Fragments arranged by size:
1394 1191 13
BmtI G_CTAG'C
Cuts at: 0 1042 2598
Size: 1042 1556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1335 1899 2598
Size: 1335 564 699
Fragments arranged by size:
1335 699 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 911 1053 1636 2376 2500 2518 2598
Size: 655 256 142 583 740 124 18 80
Fragments arranged by size:
740 655 583 256 142 124 80 18
Bpu10I CC'TnA_GC
Cuts at: 0 594 2598
Size: 594 2004
BsaI GGTCTCn'nnnn_
Cuts at: 0 257 622 2598
Size: 257 365 1976
Fragments arranged by size:
1976 365 257
BsaAI yAC'GTr
Cuts at: 0 1173 1497 2598
Size: 1173 324 1101
Fragments arranged by size:
1173 1101 324
BsaHI Gr'CG_yC
Cuts at: 0 89 308 1387 1535 1558 1861 2203 2598
Size: 89 219 1079 148 23 303 342 395
Fragments arranged by size:
1079 395 342 303 219 148 89 23
BsaWI w'CCGG_w
Cuts at: 0 939 2598
Size: 939 1659
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1922 1952 2598
Size: 1922 30 646
Fragments arranged by size:
1922 646 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 201 1603 1834 1943 2032 2362 2365 2455
Size: 201 1402 231 109 89 330 3 90
Cuts at: 2455 2464 2523 2598
Size: 9 59 75
Fragments arranged by size:
1402 330 231 201 109 90 89 75
59 9 3
BseYI C'CCAG_C
Cuts at: 0 45 131 1042 2115 2131 2558 2598
Size: 45 86 911 1073 16 427 40
Fragments arranged by size:
1073 911 427 86 45 40 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 223 353 862 2389 2598
Size: 132 91 130 509 1527 209
Fragments arranged by size:
1527 509 209 132 130 91
BsiHKAI G_wGCw'C
Cuts at: 0 560 595 743 1342 2009 2240 2401 2598
Size: 560 35 148 599 667 231 161 197
Fragments arranged by size:
667 599 560 231 197 161 148 35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2210 2598
Size: 2210 388
Bsp1286I G_dGCh'C
Cuts at: 0 41 560 595 743 1003 1342 1684 2009
Size: 41 519 35 148 260 339 342 325
Cuts at: 2009 2028 2054 2240 2401 2591 2598
Size: 19 26 186 161 190 7
Fragments arranged by size:
519 342 339 325 260 190 186 161
148 41 35 26 19 7
BspEI T'CCGG_A
Cuts at: 0 939 2598
Size: 939 1659
BspHI T'CATG_A
Cuts at: 0 1274 2598
Size: 1274 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 233 497 765 1247 1682 1722 1967
Size: 175 58 264 268 482 435 40 245
Cuts at: 1967 2598
Size: 631
Fragments arranged by size:
631 482 435 268 264 245 175 58
40
BsrBI CCG'CTC
Cuts at: 0 1572 2598
Size: 1572 1026
BsrFI r'CCGG_y
Cuts at: 0 1843 2469 2598
Size: 1843 626 129
Fragments arranged by size:
1843 626 129
BssHII G'CGCG_C
Cuts at: 0 1353 2598
Size: 1353 1245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2367 2598
Size: 2367 231
BstBI TT'CG_AA
Cuts at: 0 1754 2598
Size: 1754 844
BstEII G'GTnAC_C
Cuts at: 0 474 573 2598
Size: 474 99 2025
Fragments arranged by size:
2025 474 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 2598
Size: 993 1605
BstYI r'GATC_y
Cuts at: 0 230 692 845 2598
Size: 230 462 153 1753
Fragments arranged by size:
1753 462 230 153
Bsu36I CC'TnA_GG
Cuts at: 0 2506 2598
Size: 2506 92
BtgI C'CryG_G
Cuts at: 0 275 623 1344 1410 1421 1561 2036 2159
Size: 275 348 721 66 11 140 475 123
Cuts at: 2159 2198 2315 2598
Size: 39 117 283
Fragments arranged by size:
721 475 348 283 275 140 123 117
66 39 11
BtsI GCAGTG_nn'
Cuts at: 0 295 1436 1825 1943 2598
Size: 295 1141 389 118 655
Fragments arranged by size:
1141 655 389 295 118
DraIII CAC_nnn'GTG
Cuts at: 0 447 668 840 2598
Size: 447 221 172 1758
Fragments arranged by size:
1758 447 221 172
DrdI GACnn_nn'nnGTC
Cuts at: 0 1534 1732 1912 2598
Size: 1534 198 180 686
Fragments arranged by size:
1534 686 198 180
EaeI y'GGCC_r
Cuts at: 0 1898 2033 2598
Size: 1898 135 565
Fragments arranged by size:
1898 565 135
EarI CTCTTCn'nnn_
Cuts at: 0 767 1241 1802 2155 2269 2432 2441 2598
Size: 767 474 561 353 114 163 9 157
Fragments arranged by size:
767 561 474 353 163 157 114 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2524 2598
Size: 2524 74
EcoICRI GAG'CTC
Cuts at: 0 593 2399 2598
Size: 593 1806 199
Fragments arranged by size:
1806 593 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 655 843 911 1053 1144 1494 1636
Size: 420 235 188 68 142 91 350 142
Cuts at: 1636 2376 2500 2518 2598
Size: 740 124 18 80
Fragments arranged by size:
740 420 350 235 188 142 142 124
91 80 68 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2221 2598
Size: 2221 377
EcoO109I rG'GnC_Cy
Cuts at: 0 37 38 222 513 609 1428 1611 2024
Size: 37 1 184 291 96 819 183 413
Cuts at: 2024 2025 2051 2457 2493 2587 2598
Size: 1 26 406 36 94 11
Fragments arranged by size:
819 413 406 291 184 183 96 94
37 36 26 11 1 1
HaeII r_GCGC'y
Cuts at: 0 92 121 1372 1390 1561 1864 1984 2132
Size: 92 29 1251 18 171 303 120 148
Cuts at: 2132 2598
Size: 466
Fragments arranged by size:
1251 466 303 171 148 120 92 29
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 379 411 779 811 1780 1812
Size: 177 32 170 32 368 32 969 32
Cuts at: 1812 2598
Size: 786
Fragments arranged by size:
969 786 368 177 170 32 32 32
32
HincII GTy'rAC
Cuts at: 0 453 2598
Size: 453 2145
KasI G'GCGC_C
Cuts at: 0 88 1386 1557 1860 2598
Size: 88 1298 171 303 738
Fragments arranged by size:
1298 738 303 171 88
KpnI G_GTAC'C
Cuts at: 0 1569 2598
Size: 1569 1029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 2598
Size: 815 1783
MscI TGG'CCA
Cuts at: 0 1900 2598
Size: 1900 698
MslI CAynn'nnrTG
Cuts at: 0 622 1225 1450 2002 2197 2428 2598
Size: 622 603 225 552 195 231 170
Fragments arranged by size:
622 603 552 231 225 195 170
MspA1I CmG'CkG
Cuts at: 0 28 182 237 354 648 866 1122 1346
Size: 28 154 55 117 294 218 256 224
Cuts at: 1346 1523 1563 1739 1911 2038 2112 2358 2520
Size: 177 40 176 172 127 74 246 162
Cuts at: 2520 2526 2541 2598
Size: 6 15 57
Fragments arranged by size:
294 256 246 224 218 177 176 172
162 154 127 117 74 57 55 40
28 15 6
NaeI GCC'GGC
Cuts at: 0 2471 2598
Size: 2471 127
NarI GG'CG_CC
Cuts at: 0 89 1387 1558 1861 2598
Size: 89 1298 171 303 737
Fragments arranged by size:
1298 737 303 171 89
NcoI C'CATG_G
Cuts at: 0 2198 2315 2598
Size: 2198 117 283
Fragments arranged by size:
2198 283 117
NgoMIV G'CCGG_C
Cuts at: 0 2469 2598
Size: 2469 129
NheI G'CTAG_C
Cuts at: 0 1038 2598
Size: 1038 1560
NsiI A_TGCA'T
Cuts at: 0 589 2598
Size: 589 2009
NspI r_CATG'y
Cuts at: 0 747 951 1641 2598
Size: 747 204 690 957
Fragments arranged by size:
957 747 690 204
PasI CC'CwG_GG
Cuts at: 0 2292 2461 2598
Size: 2292 169 137
Fragments arranged by size:
2292 169 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1614 2001 2598
Size: 447 1167 387 597
Fragments arranged by size:
1167 597 447 387
PfoI T'CCnGG_A
Cuts at: 0 104 1460 1607 1721 1836 2392 2598
Size: 104 1356 147 114 115 556 206
Fragments arranged by size:
1356 556 206 147 115 114 104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1756 1788 2598
Size: 1756 32 810
Fragments arranged by size:
1756 810 32
PpuMI rG'GwC_Cy
Cuts at: 0 222 513 609 1428 1611 2457 2493 2598
Size: 222 291 96 819 183 846 36 105
Fragments arranged by size:
846 819 291 222 183 105 96 36
PspOMI G'GGCC_C
Cuts at: 0 37 2024 2050 2587 2598
Size: 37 1987 26 537 11
Fragments arranged by size:
1987 537 37 26 11
PspXI vC'TCGA_Gb
Cuts at: 0 325 2598
Size: 325 2273
PstI C_TGCA'G
Cuts at: 0 230 302 353 1443 1964 2305 2546 2598
Size: 230 72 51 1090 521 341 241 52
Fragments arranged by size:
1090 521 341 241 230 72 52 51
PvuII CAG'CTG
Cuts at: 0 182 237 354 648 866 1523 2526 2541
Size: 182 55 117 294 218 657 1003 15
Cuts at: 2541 2598
Size: 57
Fragments arranged by size:
1003 657 294 218 182 117 57 55
15
SacI G_AGCT'C
Cuts at: 0 595 2401 2598
Size: 595 1806 197
Fragments arranged by size:
1806 595 197
SacII CC_GC'GG
Cuts at: 0 1347 1564 2039 2598
Size: 1347 217 475 559
Fragments arranged by size:
1347 559 475 217
SanDI GG'GwC_CC
Cuts at: 0 513 609 2598
Size: 513 96 1989
Fragments arranged by size:
1989 513 96
SapI GCTCTTCn'nnn_
Cuts at: 0 1241 2269 2598
Size: 1241 1028 329
Fragments arranged by size:
1241 1028 329
SfcI C'TryA_G
Cuts at: 0 226 298 349 1439 1960 2301 2542 2598
Size: 226 72 51 1090 521 341 241 56
Fragments arranged by size:
1090 521 341 241 226 72 56 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1897 2032 2598
Size: 1897 135 566
Fragments arranged by size:
1897 566 135
SfoI GGC'GCC
Cuts at: 0 90 1388 1559 1862 2598
Size: 90 1298 171 303 736
Fragments arranged by size:
1298 736 303 171 90
SmaI CCC'GGG
Cuts at: 0 35 83 1685 1969 2126 2598
Size: 35 48 1602 284 157 472
Fragments arranged by size:
1602 472 284 157 48 35
SmlI C'TyrA_G
Cuts at: 0 325 2598
Size: 325 2273
SnaBI TAC'GTA
Cuts at: 0 1497 2598
Size: 1497 1101
SphI G_CATG'C
Cuts at: 0 1641 2598
Size: 1641 957
SrfI GCCC'GGGC
Cuts at: 0 1685 2126 2598
Size: 1685 441 472
Fragments arranged by size:
1685 472 441
StuI AGG'CCT
Cuts at: 0 1891 2104 2598
Size: 1891 213 494
Fragments arranged by size:
1891 494 213
StyI C'CwwG_G
Cuts at: 0 2181 2198 2315 2598
Size: 2181 17 117 283
Fragments arranged by size:
2181 283 117 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 948 1272 2598
Size: 268 680 324 1326
Fragments arranged by size:
1326 680 324 268
TatI w'GTAC_w
Cuts at: 0 962 1595 2598
Size: 962 633 1003
Fragments arranged by size:
1003 962 633
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 804 1406 1438 1922 1954 2598
Size: 772 32 602 32 484 32 644
Fragments arranged by size:
772 644 602 484 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1180 2598
Size: 1180 1418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 993 1908 2598
Size: 621 372 915 690
Fragments arranged by size:
915 690 621 372
XhoI C'TCGA_G
Cuts at: 0 325 2598
Size: 325 2273
XmaI C'CCGG_G
Cuts at: 0 33 81 1683 1967 2124 2598
Size: 33 48 1602 284 157 474
Fragments arranged by size:
1602 474 284 157 48 33
XmnI GAAnn'nnTTC
Cuts at: 0 935 2598
Size: 935 1663
ZraI GAC'GTC
Cuts at: 0 1536 2598
Size: 1536 1062
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AleI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BfrBI BglI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI SfcI SfiI
SfoI SmaI SmlI SnaBI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BglII BlpI BpuEI BsaBI BsiEI
BsiWI BsmI BsrDI BsrGI BssSI BstZ17I BtgZI ClaI
CspCI DraI EagI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI MfeI MluI NdeI NotI NruI
PacI PciI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SgfI SgrAI SpeI
SspI SwaI XbaI