Restriction map for
pFN21AB6756
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BcnI BfaI
BfrI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 421 CGCG CG^CG
AfaI 228 GTAC GT^AC
AflIII 257 ACGTGT A^C[GA][CT]GT
AlwNI 101 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 126 CCTCAGG CC^T[ACGT]AGG
AspHI 133 GAGCTC G[AT]GC[AT]^C
AvaII 390 GGACC G^G[AT]CC
BanII 133 GAGCTC G[GA]GC[CT]^C
BbsI 282 GAAGAC GAAGAC(2/6)
BcgI 269 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 251 TGATCA T^GATCA
BglI 80 GCCTTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 297 GCTGAGC GC^T[ACGT]AGC
BmyI 133 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
BpuAI 282 GAAGAC GAAGAC(2/6)
BsaI 321 GGTCTC GGTCTC(1/5)
BsgI 233 GTGCAG GTGCAG(16/14)
BsiHKAI 133 GAGCTC G[AT]GC[AT]^C
BsiYI 312 CCCGCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 312 CCCGCACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 426 GGGAC GGGAC(10/14)
Bsp1286I 133 GAGCTC G[AGT]GC[ACT]^C
BspMI 392 ACCTGC ACCTGC(4/8)
BstUI 421 CGCG CG^CG
Bsu36I 126 CCTCAGG CC^T[ACGT]AGG
CelII 297 GCTGAGC GC^T[ACGT]AGC
CfoI 46 GCGC GCG^C
DpnII 252 GATC ^GATC
DraI 405 TTTAAA TTT^AAA
DsaI 59 CCACGG C^C[GA][CT]GG
Eco81I 126 CCTCAGG CC^T[ACGT]AGG
EcoT14I 81 CCTTGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FauI 312 CCCGC CCCGC(4/6)
FbaI 251 TGATCA T^GATCA
HaeII 45 GGCGCT [GA]GCGC^[CT]
HhaI 46 GCGC GCG^C
HinP1I 46 GCGC G^CGC
HphI 158 TCACC GGTGA(8/7)
MaeII 257 ACGT A^CGT
MboI 252 GATC ^GATC
MboII 282 GAAGA GAAGA(8/7)
MseI 406 TTAA T^TAA
MvnI 421 CGCG CG^CG
NdeII 252 GATC ^GATC
NlaIV 174 GGCTCC GG[ACGT]^[ACGT]CC
NruI 420 TCGCGA TCG^CGA
NsiI 1 ATGCAT ATGCA^T
PpuMI 389 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 57 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 267 CTGCAG CTGCA^G
RsaI 228 GTAC GT^AC
SacI 133 GAGCTC GAGCT^C
Sau3AI 252 GATC ^GATC
StyI 81 CCTTGG C^C[AT][AT]GG
Tru9I 406 TTAA T^TAA
XcmI 362 CCAAAATCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BseRI 10 CTCCTC GAGGAG(10/8)
142 GAGGAG
BsmAI 356 GAGAC GTCTC(1/5)
322 GTCTC
BsrDI 66 CATTGC GCAATG(2/0)
185 GCAATG
BsrI 33 ACTGG ACTGG(1/-1)
378 CCAGT
BssKI 100 CCAGG ^CC[ACGT]GG
240 CCTGG
BstF5I 4 CATCC GGATG(2/0)
293 GGATG
BstNI 100 CCAGG CC^[AT]GG
240 CCTGG
DraII 102 AGGCCCT [GA]G^G[ACGT]CC[CT]
389 AGGACCT
EcoO109I 102 AGGCCCT [GA]G^G[ACGT]CC[CT]
389 AGGACCT
EcoRII 100 CCAGG ^CC[AT]GG
240 CCTGG
FokI 4 CATCC GGATG(9/13)
293 GGATG
HincII 54 GTTGAC GT[CT]^[GA]AC
151 GTCAAC
HindII 54 GTTGAC GT[CT]^[GA]AC
151 GTCAAC
HinfI 244 GAATC G^A[ACGT]TC
305 GATTC
MspA1I 218 CAGCTG C[AC]G^C[GT]G
326 CAGCTG
MvaI 100 CCAGG CC^[AT]GG
240 CCTGG
NspI 224 GCATGT [GA]CATG^[CT]
348 GCATGT
PvuII 218 CAGCTG CAG^CTG
326 CAGCTG
ScrFI 100 CCAGG CC^[ACGT]GG
240 CCTGG
SfcI 119 CTACAG C^T[GA][CT]AG
267 CTGCAG
TaqI 278 TCGA T^CGA
368 TCGA
TfiI 244 GAATC G^A[AT]TC
305 GATTC
TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^
270 CAGTG
TthHB8I 278 TCGA T^CGA
368 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsrBI
Cac8I Cfr13I DdeI Fnu4HI HaeIII
ItaI MnlI MwoI NlaIII Sau96I
SfaNI TseI