Restriction map for
pFN21AB6480
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspHI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MscI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 943 GACGTC GACGT^C
AccI 184 GTCTAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AccIII 975 TCCGGA T^CCGGA
AcyI 943 GACGTC G[GA]^CG[CT]C
AlwNI 133 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 359 GAGCAC G[AT]GC[AT]^C
BbsI 43 GTCTTC GAAGAC(2/6)
BglI 55 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 349 GCTGAGC GC^T[ACGT]AGC
BmyI 359 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 349 GCTGAGC GC^T[ACGT]AGC
BpuAI 43 GTCTTC GAAGAC(2/6)
BsaHI 943 GACGTC G[GA]^CG[CT]C
BsaWI 975 TCCGGA [AT]^CCGG[AT]
BseAI 975 TCCGGA T^CCGGA
BsgI 193 GTGCAG GTGCAG(16/14)
BsiHKAI 359 GAGCAC G[AT]GC[AT]^C
Bsp1286I 359 GAGCAC G[AGT]GC[ACT]^C
BspEI 975 TCCGGA T^CCGGA
BspMI 132 GCAGGT ACCTGC(4/8)
BsrBI 92 CCGCTC CCGCTC(-3/-3)
BssSI 171 CACGAG CACGAG(-5/-1)
BstEII 441 GGTGACC G^GT[ACGT]ACC
BstPI 441 GGTGACC G^GT[ACGT]ACC
BstUI 60 CGCG CG^CG
BstXI 587 CCATTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 349 GCTGAGC GC^T[ACGT]AGC
EaeI 227 CGGCCG [CT]^GGCC[GA]
EagI 227 CGGCCG C^GGCCG
EarI 874 GAAGAG CTCTTC(1/4)
EclXI 227 CGGCCG C^GGCCG
Eco52I 227 CGGCCG C^GGCCG
EcoO65I 441 GGTGACC G^GT[ACGT]ACC
HapII 976 CCGG C^CGG
HgaI 9 GACGC GACGC(5/10)
Hin1I 943 GACGTC G[GA]^CG[CT]C
HpaII 976 CCGG C^CGG
Ksp632I 874 GAAGAG CTCTTC(1/4)
KspI 59 CCGCGG CCGC^GG
MaeII 944 ACGT A^CGT
MroI 975 TCCGGA T^CCGGA
MspI 976 CCGG C^CGG
MvnI 60 CGCG CG^CG
NlaIII 237 CATG CATG^
PpuMI 134 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 85 CAGCTG CAG^CTG
SacII 59 CCGCGG CCGC^GG
TaqI 345 TCGA T^CGA
Tsp509I 253 AATT ^AATT
TthHB8I 345 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 232 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
952 GACTCTGTGTC
Asp700I 761 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
991 GAAGATGTTC
AspEI 232 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
952 GACTCTGTGTC
AvaII 135 GGTCC G^G[AT]CC
807 GGTCC
BbvCI 97 CCTCAGC CCTCAGC(-5/-2)
302 CCTCAGC
BpmI 425 CTCCAG CTGGAG(16/14)
612 CTCCAG
BseRI 95 CTCCTC GAGGAG(10/8)
477 CTCCTC
BsiEI 4 CGACCG CG[GA][CT]^CG
227 CGGCCG
BsmFI 950 GGGAC GGGAC(10/14)
299 GTCCC
Bsp1407I 119 TGTACA T^GTACA
256 TGTACA
BsrGI 119 TGTACA T^GTACA
256 TGTACA
DpnII 264 GATC ^GATC
750 GATC
DraI 199 TTTAAA TTT^AAA
882 TTTAAA
DraII 134 AGGTCCT [GA]G^G[ACGT]CC[CT]
567 AGGCCCT
Eam1105I 232 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
952 GACTCTGTGTC
EcoO109I 134 AGGTCCT [GA]G^G[ACGT]CC[CT]
567 AGGCCCT
EcoT14I 388 CCTTGG C^C[AT][AT]GG
914 CCTTGG
FauI 66 CCCGC CCCGC(4/6)
91 CCCGC
MboI 264 GATC ^GATC
750 GATC
MslI 243 CATAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
435 CATTGAGGTG
MspA1I 59 CCGCGG C[AC]G^C[GT]G
85 CAGCTG
NdeII 264 GATC ^GATC
750 GATC
Sau3AI 264 GATC ^GATC
750 GATC
SfaNI 718 GATGC GCATC(5/9)
857 GCATC
SspBI 119 TGTACA T^GTACA
256 TGTACA
StyI 388 CCTTGG C^C[AT][AT]GG
914 CCTTGG
TfiI 111 GATTC G^A[AT]TC
551 GAATC
XmnI 761 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
991 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DsaI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MseI MvaI MwoI NlaIV PleI
RsaI Sau96I ScrFI StuI Tru9I
TseI Tsp45I TspRI