(Circular) (Six-base) MAPSORT of: pFN21AE0422.seq Check: 6574 from: 1 to: 5884 pFN21AE0422 5884 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3141 3694 278 Size: 53 83 186 936 1605 553 2468 Fragments arranged by size: 2468 1605 936 553 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2218 2366 2591 3144 3947 5331 5599 Size: 401 185 148 225 553 803 1384 268 Cuts at: 5599 1632 Size: 1917 Fragments arranged by size: 1917 1384 803 553 401 268 225 185 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 2244 1166 Size: 1078 4806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3051 1184 Size: 1867 4017 AlwNI CAG_nnn'CTG Cuts at: 3627 5199 3627 Size: 1572 4312 ApaI G_GGCC'C Cuts at: 2876 2876 Size: 5884 ApaLI G'TGCA_C Cuts at: 2270 2660 3941 5097 2270 Size: 390 1281 1156 3057 Fragments arranged by size: 3057 1281 1156 390 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2110 3209 1870 Size: 73 111 56 1099 4545 Fragments arranged by size: 4545 1099 111 73 56 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 3205 4660 618 Size: 325 217 627 306 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2211 2876 3203 1391 Size: 820 665 327 4072 Fragments arranged by size: 4072 820 665 327 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5884 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 4588 Fragments arranged by size: 4588 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 2375 2409 2935 2969 4075 Size: 34 95 34 309 34 526 34 1106 Cuts at: 4075 4109 1903 Size: 34 3678 Fragments arranged by size: 3678 1106 526 309 95 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2045 2318 2460 Size: 107 122 71 631 605 373 273 142 Cuts at: 2460 3179 4495 136 Size: 719 1316 1525 Fragments arranged by size: 1525 1316 719 631 605 373 273 142 122 107 71 BglII A'GATC_T Cuts at: 5879 5879 Size: 5884 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2274 2664 2876 3116 3945 5101 1790 Size: 484 390 212 240 829 1156 2573 Fragments arranged by size: 2573 1156 829 484 390 240 212 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2693 3615 4573 Size: 416 53 83 399 24 1276 922 958 Cuts at: 4573 442 Size: 1753 Fragments arranged by size: 1753 1276 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2125 2125 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 2687 4544 1239 Size: 1316 132 1857 2579 Fragments arranged by size: 2579 1857 1316 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 4009 4874 5172 3005 Size: 1004 865 298 3717 Fragments arranged by size: 3717 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4547 882 Size: 617 3048 2219 Fragments arranged by size: 3048 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2275 3675 493 Size: 711 1071 1400 2702 Fragments arranged by size: 2702 1400 1071 711 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 3138 3691 4073 Size: 53 83 186 936 561 1044 553 382 Cuts at: 4073 4708 275 Size: 635 1451 Fragments arranged by size: 1451 1044 936 635 561 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3094 4317 4715 4989 5136 5547 3094 Size: 1223 398 274 147 411 3431 Fragments arranged by size: 3431 1223 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2125 2155 1178 Size: 30 165 30 722 30 4907 Fragments arranged by size: 4907 722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5884 BseYI C'CCAG_C Cuts at: 2796 2888 5087 5583 2796 Size: 92 2199 496 3097 Fragments arranged by size: 3097 2199 496 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3011 1163 Size: 573 1275 4036 Fragments arranged by size: 4036 1275 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2658 3169 3334 4095 5123 Size: 275 186 24 633 511 165 761 1028 Cuts at: 5123 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 633 511 275 186 165 24 BsiHKAI G_wGCw'C Cuts at: 2055 2211 2274 2664 3945 4030 5101 2055 Size: 156 63 390 1281 85 1071 2838 Fragments arranged by size: 2838 1281 1071 390 156 85 63 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2211 2274 2664 2876 3116 3203 Size: 399 265 156 63 390 212 240 87 Cuts at: 3203 3945 4030 5101 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 265 240 212 156 87 85 63 BspEI T'CCGG_A Cuts at: 3094 5547 3094 Size: 2453 3431 BspHI T'CATG_A Cuts at: 2676 3773 5503 2676 Size: 1097 1730 3057 Fragments arranged by size: 3057 1730 1097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 3239 844 Size: 1538 857 3489 Fragments arranged by size: 3489 1538 857 BsrBI CCG'CTC Cuts at: 1394 1823 2637 3771 1394 Size: 429 814 1134 3507 Fragments arranged by size: 3507 1134 814 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2314 2601 4379 4553 65 Size: 1183 1066 287 1778 174 1396 Fragments arranged by size: 1778 1396 1183 1066 287 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2942 4528 4715 1700 Size: 110 220 912 1586 187 2869 Fragments arranged by size: 2869 1586 912 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 2099 3944 4956 2099 Size: 1845 1012 3027 Fragments arranged by size: 3027 1845 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5884 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 2173 2467 1184 Size: 989 294 4601 Fragments arranged by size: 4601 989 294 BstXI CCAn_nnnn'nTGG Cuts at: 2782 2992 2782 Size: 210 5674 BstYI r'GATC_y Cuts at: 1972 3214 3581 3966 3983 5435 5521 5533 5879 Size: 1242 367 385 17 1452 86 12 346 Cuts at: 5879 1972 Size: 1977 Fragments arranged by size: 1977 1452 1242 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2979 3069 3174 513 Size: 552 939 975 90 105 3223 Fragments arranged by size: 3223 975 939 552 105 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2703 2953 487 Size: 683 56 1477 250 3418 Fragments arranged by size: 3418 1477 683 250 56 BtsI GCAGTG_nn' Cuts at: 3400 4194 4214 3400 Size: 794 20 5070 Fragments arranged by size: 5070 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DraIII CAC_nnn'GTG Cuts at: 2173 2173 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2655 2902 Size: 54 1425 50 275 18 489 336 247 Cuts at: 2902 2982 2994 3166 3331 4220 5849 8 Size: 80 12 172 165 889 1629 43 Fragments arranged by size: 1629 1425 889 489 336 275 247 172 165 80 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2655 3166 3331 1537 Size: 275 843 511 165 4090 Fragments arranged by size: 4090 843 511 275 165 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2289 3814 1353 Size: 906 30 1525 3423 Fragments arranged by size: 3423 1525 906 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2315 3134 4471 4845 4991 387 Size: 1064 138 726 819 1337 374 146 1280 Fragments arranged by size: 1337 1280 1064 819 726 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 2209 Size: 5884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2218 2366 2555 2591 2687 3144 Size: 393 401 185 148 189 36 96 457 Cuts at: 3144 3947 4544 5331 5599 1239 Size: 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 597 457 401 393 268 189 185 148 96 36 EcoO109I rG'GnC_Cy Cuts at: 2104 2872 2104 Size: 768 5116 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 5884 FspI TGC'GCA Cuts at: 2600 4390 2600 Size: 1790 4094 HaeII r_GCGC'y Cuts at: 1678 2012 2040 2097 5031 1678 Size: 334 28 57 2934 2531 Fragments arranged by size: 2934 2531 334 57 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2809 2841 4525 4557 4599 Size: 32 135 32 1237 32 1684 32 42 Cuts at: 4599 4631 1373 Size: 32 2626 Fragments arranged by size: 2626 1684 1237 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2845 3228 3474 669 Size: 988 281 907 383 246 3079 Fragments arranged by size: 3079 988 907 383 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2904 2996 10 Size: 54 1425 343 489 583 92 2898 Fragments arranged by size: 2898 1425 583 489 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 2244 2544 2759 2774 2816 3033 3842 Size: 648 1078 300 215 15 42 217 809 Cuts at: 3842 4201 4360 518 Size: 359 159 2042 Fragments arranged by size: 2042 1078 809 648 359 300 217 215 159 42 15 MspA1I CmG'CkG Cuts at: 1606 2782 3000 3300 3977 5125 5370 1606 Size: 1176 218 300 677 1148 245 2120 Fragments arranged by size: 2120 1176 1148 677 300 245 218 NaeI GCC'GGC Cuts at: 2032 2032 Size: 5884 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5884 NcoI C'CATG_G Cuts at: 513 1065 2004 3069 513 Size: 552 939 1065 3328 Fragments arranged by size: 3328 1065 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NgoMIV G'CCGG_C Cuts at: 2030 2030 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2222 2247 3242 4787 1132 Size: 97 993 25 995 1545 2229 Fragments arranged by size: 2229 1545 995 993 97 25 PasI CC'CwG_GG Cuts at: 1348 2079 2186 1348 Size: 731 107 5046 Fragments arranged by size: 5046 731 107 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2192 1108 Size: 1084 4800 PfoI T'CCnGG_A Cuts at: 2502 3132 2502 Size: 630 5254 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PmlI CAC'GTG Cuts at: 2275 2275 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2104 Size: 5884 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 2872 2872 Size: 5884 PspXI vC'TCGA_Gb Cuts at: 2110 2110 Size: 5884 PstI C_TGCA'G Cuts at: 830 2714 3236 830 Size: 1884 522 3478 Fragments arranged by size: 3478 1884 522 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2782 3000 2782 Size: 218 5666 SacI G_AGCT'C Cuts at: 2211 2211 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2710 3232 4367 5048 5239 826 Size: 219 1665 522 1135 681 191 1471 Fragments arranged by size: 1665 1471 1135 681 522 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3179 3179 Size: 5884 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5884 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 3211 1872 Size: 1339 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2110 3020 3988 4889 5151 820 Size: 197 926 167 910 968 901 262 1553 Fragments arranged by size: 1553 968 926 910 901 262 197 167 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 2880 1517 Size: 1363 4521 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 3069 3323 513 Size: 552 939 579 486 254 3074 Fragments arranged by size: 3074 939 579 552 486 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 3928 4081 4266 4711 325 Size: 1255 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 4130 5842 Size: 274 80 33 51 1443 546 1607 1712 Cuts at: 5842 96 Size: 138 Fragments arranged by size: 1712 1607 1443 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 5844 5876 2264 Size: 32 3548 32 2272 Fragments arranged by size: 3548 2272 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2779 2779 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 2110 1943 Size: 167 5717 XmaI C'CCGG_G Cuts at: 1870 3209 1870 Size: 1339 4545 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3139 3692 276 Size: 53 83 186 936 1605 553 2468 Fragments arranged by size: 2468 1605 936 553 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI