(Circular) (Six-base) MAPSORT of: pFN21AB5952.seq Check: 7674 from: 1 to: 6346 pFN21AB5952 6346 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2207 4156 278 Size: 53 83 186 936 671 1949 2468 Fragments arranged by size: 2468 1949 936 671 186 83 53 AccI GT'mk_AC Cuts at: 1937 2766 3689 1937 Size: 829 923 4594 Fragments arranged by size: 4594 923 829 Acc65I G'GTAC_C Cuts at: 2017 3667 2017 Size: 1650 4696 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 3517 4409 5793 6061 1632 Size: 1436 449 892 1384 268 1917 Fragments arranged by size: 1917 1436 1384 892 449 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 3259 5245 6222 1203 Size: 2056 1986 977 1327 Fragments arranged by size: 2056 1986 1327 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 2459 1166 Size: 1293 5053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3602 1184 Size: 2418 3928 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3140 3172 3140 Size: 32 6314 AlwNI CAG_nnn'CTG Cuts at: 2043 2605 2791 4089 5661 2043 Size: 562 186 1298 1572 2728 Fragments arranged by size: 2728 1572 1298 562 186 ApaLI G'TGCA_C Cuts at: 4403 5559 4403 Size: 1156 5190 ApoI r'AATT_y Cuts at: 1493 3655 3881 4033 6142 1493 Size: 2162 226 152 2109 1697 Fragments arranged by size: 2162 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 2871 2919 3024 3064 3140 3296 3671 Size: 73 928 48 105 40 76 156 375 Cuts at: 3671 1870 Size: 4545 Fragments arranged by size: 4545 928 375 156 105 76 73 48 40 AvrII C'CTAG_G Cuts at: 2896 2947 2896 Size: 51 6295 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2001 2034 2001 Size: 33 6313 BamHI G'GATC_C Cuts at: 2283 3345 3676 4043 2283 Size: 1062 331 367 4586 Fragments arranged by size: 4586 1062 367 331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2371 2867 3334 3667 Size: 325 217 627 230 354 496 467 333 Cuts at: 3667 5122 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 496 467 354 333 325 230 217 BanII G_rGCy'C Cuts at: 1391 2118 2563 2976 3025 3455 3665 1391 Size: 727 445 413 49 430 210 4072 Fragments arranged by size: 4072 727 445 430 413 210 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3463 3523 928 Size: 971 1564 60 3751 Fragments arranged by size: 3751 1564 971 60 BbvCI CC'TCA_GC Cuts at: 2626 2966 3642 2626 Size: 340 676 5330 Fragments arranged by size: 5330 676 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3204 3238 4537 4571 1903 Size: 34 1267 34 1299 34 3678 Fragments arranged by size: 3678 1299 1267 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2386 2602 2756 Size: 107 122 71 631 605 714 216 154 Cuts at: 2756 4957 136 Size: 2201 1525 Fragments arranged by size: 2201 1525 714 631 605 216 154 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 2486 3763 2486 Size: 1277 5069 Bme1580I G_kGCm'C Cuts at: 1790 2872 4407 5563 1790 Size: 1082 1535 1156 2573 Fragments arranged by size: 2573 1535 1156 1082 BmgBI CAC'GTC Cuts at: 1318 3262 3597 1318 Size: 1944 335 4067 Fragments arranged by size: 4067 1944 335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4077 5035 442 Size: 416 53 83 399 24 2660 958 1753 Fragments arranged by size: 2660 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 3307 5006 1239 Size: 921 1147 1699 2579 Fragments arranged by size: 2579 1699 1147 921 Bpu10I CC'TnA_GC Cuts at: 2626 2966 3642 2626 Size: 340 676 5330 Fragments arranged by size: 5330 676 340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4471 5336 5634 4471 Size: 865 298 5183 Fragments arranged by size: 5183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4137 493 Size: 711 2933 2702 Fragments arranged by size: 2933 2702 711 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 4153 4535 5170 Size: 53 83 186 936 671 1949 382 635 Cuts at: 5170 275 Size: 1451 Fragments arranged by size: 1949 1451 936 671 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4779 5177 5451 5598 6009 4779 Size: 398 274 147 411 5116 Fragments arranged by size: 5116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2420 2450 3541 3571 1178 Size: 30 165 30 1017 30 1091 30 3953 Fragments arranged by size: 3953 1091 1017 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2317 2492 2495 2522 3587 1186 Size: 1131 175 3 27 1065 3945 Fragments arranged by size: 3945 1131 1065 175 27 3 BseYI C'CCAG_C Cuts at: 2177 2241 2472 2605 2776 3017 3228 3446 5549 Size: 64 231 133 171 241 211 218 2103 Cuts at: 5549 6045 2177 Size: 496 2478 Fragments arranged by size: 2478 2103 496 241 231 218 211 171 133 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2710 1163 Size: 573 974 4799 Fragments arranged by size: 4799 974 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3057 3796 4557 5585 1540 Size: 275 186 1056 739 761 1028 2301 Fragments arranged by size: 2301 1056 1028 761 739 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 3455 3543 3581 4407 4492 5563 2133 Size: 1322 88 38 826 85 1071 2916 Fragments arranged by size: 2916 1322 1071 826 88 85 38 BsmI GAATG_Cn' Cuts at: 2800 3857 3950 2800 Size: 1057 93 5196 Fragments arranged by size: 5196 1057 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2195 2541 2902 2195 Size: 346 361 5639 Fragments arranged by size: 5639 361 346 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2133 2563 2872 2976 3025 3455 Size: 399 328 15 430 309 104 49 430 Cuts at: 3455 3543 3581 3665 4407 4492 5563 1391 Size: 88 38 84 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 430 430 399 328 309 104 88 85 84 49 38 15 BspEI T'CCGG_A Cuts at: 6009 6009 Size: 6346 BspHI T'CATG_A Cuts at: 2805 4235 5965 2805 Size: 1430 1730 3186 Fragments arranged by size: 3186 1730 1430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 844 Size: 2857 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3552 4233 1394 Size: 429 1729 681 3507 Fragments arranged by size: 3507 1729 681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 3624 4841 5015 65 Size: 1183 1063 1313 1217 174 1396 Fragments arranged by size: 1396 1313 1217 1183 1063 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2392 2490 2716 3337 4990 5177 1700 Size: 110 582 98 226 621 1653 187 2869 Fragments arranged by size: 2869 1653 621 582 226 187 110 98 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 4099 BssHII G'CGCG_C Cuts at: 1925 2849 2851 1925 Size: 924 2 5420 Fragments arranged by size: 5420 924 2 BssSI C'ACGA_G Cuts at: 2678 3405 4406 5418 2678 Size: 727 1001 1012 3606 Fragments arranged by size: 3606 1012 1001 727 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6346 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6346 BstXI CCAn_nnnn'nTGG Cuts at: 2236 2536 2236 Size: 300 6046 BstYI r'GATC_y Cuts at: 1972 2283 3345 3676 4043 4428 4445 5897 5983 Size: 311 1062 331 367 385 17 1452 86 Cuts at: 5983 5995 6341 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1062 385 367 346 331 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2554 2812 2844 3057 513 Size: 552 939 9 541 258 32 213 3802 Fragments arranged by size: 3802 939 552 541 258 213 32 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 2508 2523 3136 3275 3862 4656 4676 2508 Size: 15 613 139 587 794 20 4178 Fragments arranged by size: 4178 794 613 587 139 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5353 809 Size: 507 4037 1802 Fragments arranged by size: 4037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2226 2538 Size: 54 1425 50 275 18 177 219 312 Cuts at: 2538 2714 2829 3054 3793 4682 6311 8 Size: 176 115 225 739 889 1629 43 Fragments arranged by size: 1629 1425 889 739 312 275 225 219 177 176 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3054 3793 1537 Size: 275 1242 739 4090 Fragments arranged by size: 4090 1242 739 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4276 1353 Size: 2923 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3453 3453 Size: 6346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 3068 3307 3517 4409 5006 5793 Size: 393 528 908 239 210 892 597 787 Cuts at: 5793 6061 1239 Size: 268 1524 Fragments arranged by size: 1524 908 892 787 597 528 393 268 239 210 EcoNI CCTnn'n_nnAGG Cuts at: 2213 2213 Size: 6346 EcoO109I rG'GnC_Cy Cuts at: 2040 2145 2951 2040 Size: 105 806 5435 Fragments arranged by size: 5435 806 105 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 2777 5493 1678 Size: 1099 2716 2531 Fragments arranged by size: 2716 2531 1099 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 3018 3050 3568 Size: 32 135 32 848 32 566 32 518 Cuts at: 3568 3600 4987 5019 5061 5093 1373 Size: 32 1387 32 42 32 2626 Fragments arranged by size: 2626 1387 848 566 518 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 3690 3936 669 Size: 988 281 1423 329 246 3079 Fragments arranged by size: 3079 1423 988 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6346 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 2021 3671 2021 Size: 1650 4696 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2261 5460 5644 1305 Size: 956 3199 184 2007 Fragments arranged by size: 3199 2007 956 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2228 2540 10 Size: 54 1425 343 177 219 312 3816 Fragments arranged by size: 3816 1425 343 312 219 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 2459 3599 4304 4663 4822 518 Size: 648 1173 120 1140 705 359 159 2042 Fragments arranged by size: 2042 1173 1140 705 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2516 3059 3184 3762 4439 5587 5832 1606 Size: 910 543 125 578 677 1148 245 2120 Fragments arranged by size: 2120 1148 910 677 578 543 245 125 NaeI GCC'GGC Cuts at: 2492 2492 Size: 6346 NcoI C'CATG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4846 Fragments arranged by size: 4846 939 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NgoMIV G'CCGG_C Cuts at: 2490 2490 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 2593 3225 3704 5249 1132 Size: 97 1364 632 479 1545 2229 Fragments arranged by size: 2229 1545 1364 632 479 97 PasI CC'CwG_GG Cuts at: 1348 2037 2599 3493 1348 Size: 689 562 894 4201 Fragments arranged by size: 4201 894 689 562 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 4849 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 6346 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2145 2951 2145 Size: 806 5540 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PspXI vC'TCGA_Gb Cuts at: 3024 3024 Size: 6346 PstI C_TGCA'G Cuts at: 830 2165 3131 3270 3698 830 Size: 1335 966 139 428 3478 Fragments arranged by size: 3478 1335 966 428 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 3184 3184 Size: 6346 SacI G_AGCT'C Cuts at: 3455 3455 Size: 6346 SacII CC_GC'GG Cuts at: 3060 3060 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SanDI GG'GwC_CC Cuts at: 2145 2145 Size: 6346 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 2167 4594 6306 2167 Size: 2427 1712 2207 Fragments arranged by size: 2427 2207 1712 SfcI C'TryA_G Cuts at: 826 1045 2161 3127 3266 3694 4829 5510 5701 Size: 219 1116 966 139 428 1135 681 191 Cuts at: 5701 826 Size: 1471 Fragments arranged by size: 1471 1135 1116 966 681 428 219 191 139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 2873 2921 3066 3673 1872 Size: 1001 48 145 607 4545 Fragments arranged by size: 4545 1001 607 145 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 3024 3140 4450 5351 5613 820 Size: 197 926 1081 116 1310 901 262 1553 Fragments arranged by size: 1553 1310 1081 926 901 262 197 116 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2196 2384 2896 2947 3785 Size: 552 939 9 183 188 512 51 838 Cuts at: 3785 513 Size: 3074 Fragments arranged by size: 3074 939 838 552 512 188 183 51 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3228 4373 4390 4543 4728 5173 325 Size: 1255 1648 1145 17 153 185 445 1498 Fragments arranged by size: 1648 1498 1255 1145 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2165 2298 2343 Size: 274 80 33 51 1443 188 133 45 Cuts at: 2343 4592 6304 96 Size: 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 188 138 133 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 2696 2728 2943 2975 6306 6338 2601 Size: 32 63 32 215 32 3331 32 2609 Fragments arranged by size: 3331 2609 215 63 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2028 2429 2455 3142 2011 Size: 17 401 26 687 5215 Fragments arranged by size: 5215 687 401 26 17 XhoI C'TCGA_G Cuts at: 1943 3024 3140 1943 Size: 1081 116 5149 Fragments arranged by size: 5149 1081 116 XmaI C'CCGG_G Cuts at: 1870 2871 2919 3064 3671 1870 Size: 1001 48 145 607 4545 Fragments arranged by size: 4545 1001 607 145 48 XmnI GAAnn'nnTTC Cuts at: 4475 4475 Size: 6346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2205 4154 276 Size: 53 83 186 936 671 1949 2468 Fragments arranged by size: 2468 1949 936 671 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI