Restriction map for
pFN21AB6308
(Linear) (Six-base) MAPSORT of: KIBB6308.seq Check: 4096 from: 1 to: 840
KIBB6308.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 799 840
Size: 704 95 41
Fragments arranged by size:
704 95 41
AflIII A'CryG_T
Cuts at: 0 806 840
Size: 806 34
AgeI A'CCGG_T
Cuts at: 0 444 840
Size: 444 396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 840
Size: 434 32 374
Fragments arranged by size:
434 374 32
AlwNI CAG_nnn'CTG
Cuts at: 0 460 840
Size: 460 380
ApaLI G'TGCA_C
Cuts at: 0 688 840
Size: 688 152
ApoI r'AATT_y
Cuts at: 0 196 570 840
Size: 196 374 270
Fragments arranged by size:
374 270 196
AvaI C'yCGr_G
Cuts at: 0 266 840
Size: 266 574
AvrII C'CTAG_G
Cuts at: 0 415 840
Size: 415 425
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 496 809 840
Size: 283 213 313 31
Fragments arranged by size:
313 283 213 31
BglII A'GATC_T
Cuts at: 0 618 840
Size: 618 222
Bme1580I G_kGCm'C
Cuts at: 0 692 840
Size: 692 148
BmgBI CAC'GTC
Cuts at: 0 807 840
Size: 807 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 840
Size: 231 609
BsaI GGTCTCn'nnnn_
Cuts at: 0 348 840
Size: 348 492
BsaWI w'CCGG_w
Cuts at: 0 444 840
Size: 444 396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 840
Size: 164 676
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 840
Size: 72 768
BsiHKAI G_wGCw'C
Cuts at: 0 692 840
Size: 692 148
BsmI GAATG_Cn'
Cuts at: 0 294 840
Size: 294 546
Bsp1286I G_dGCh'C
Cuts at: 0 692 840
Size: 692 148
BspHI T'CATG_A
Cuts at: 0 798 840
Size: 798 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 840
Size: 94 746
BsrFI r'CCGG_y
Cuts at: 0 444 840
Size: 444 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 840
Size: 21 819
BstYI r'GATC_y
Cuts at: 0 456 618 840
Size: 456 162 222
Fragments arranged by size:
456 222 162
BtgI C'CryG_G
Cuts at: 0 347 840
Size: 347 493
DraI TTT'AAA
Cuts at: 0 615 840
Size: 615 225
EarI CTCTTCn'nnn_
Cuts at: 0 493 840
Size: 493 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 799 840
Size: 704 95 41
Fragments arranged by size:
704 95 41
EcoO109I rG'GnC_Cy
Cuts at: 0 29 840
Size: 29 811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 840
Size: 135 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 343 375 840
Size: 235 32 76 32 465
Fragments arranged by size:
465 235 76 32 32
HindIII A'AGCT_T
Cuts at: 0 73 112 840
Size: 73 39 728
Fragments arranged by size:
728 73 39
MslI CAynn'nnrTG
Cuts at: 0 406 840
Size: 406 434
NspI r_CATG'y
Cuts at: 0 162 840
Size: 162 678
PstI C_TGCA'G
Cuts at: 0 222 840
Size: 222 618
SfcI C'TryA_G
Cuts at: 0 218 712 840
Size: 218 494 128
Fragments arranged by size:
494 218 128
SmlI C'TyrA_G
Cuts at: 0 266 840
Size: 266 574
SspI AAT'ATT
Cuts at: 0 762 840
Size: 762 78
StuI AGG'CCT
Cuts at: 0 751 840
Size: 751 89
StyI C'CwwG_G
Cuts at: 0 415 840
Size: 415 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 840
Size: 667 173
XhoI C'TCGA_G
Cuts at: 0 266 840
Size: 266 574
XmnI GAAnn'nnTTC
Cuts at: 0 43 187 840
Size: 43 144 653
Fragments arranged by size:
653 144 43
Enzymes that do cut:
AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AvaI
AvrII BbsI BglII Bme1580I BmgBI BplI BsaI BsaWI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BstXI BstYI BtgI DraI EarI Eco57MI EcoO109I FalI
Hin4I HindIII MslI NspI PstI SfcI SmlI SspI
StuI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI ApaI AscI AseI AsiSI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI TstI Tth111I XbaI XcmI XmaI ZraI