Restriction map for
pFN21AE0342
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaI BsgI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EarI
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 262 GACGTC GACGT^C
AccII 379 CGCG CG^CG
AgeI 1169 ACCGGT A^CCGGT
Alw44I 835 GTGCAC G^TGCAC
Aor51HI 352 AGCGCT AGC^GCT
ApaLI 835 GTGCAC G^TGCAC
Asp700I 867 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 953 TGCGCA TGC^GCA
BbsI 1035 GAAGAC GAAGAC(2/6)
BbvCI 163 CCTCAGC CCTCAGC(-5/-2)
BglI 1133 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 212 AGATCT A^GATCT
BpuAI 1035 GAAGAC GAAGAC(2/6)
BsaBI 1743 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1690 CGGCCG CG[GA][CT]^CG
BsrBI 553 GAGCGG CCGCTC(-3/-3)
BsrDI 984 CATTGC GCAATG(2/0)
BstEII 513 GGTGACC G^GT[ACGT]ACC
BstPI 513 GGTGACC G^GT[ACGT]ACC
BstUI 379 CGCG CG^CG
DraI 217 TTTAAA TTT^AAA
EagI 1690 CGGCCG C^GGCCG
EclXI 1690 CGGCCG C^GGCCG
Eco47III 352 AGCGCT AGC^GCT
Eco52I 1690 CGGCCG C^GGCCG
EcoNI 114 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 513 GGTGACC G^GT[ACGT]ACC
FspI 953 TGCGCA TGC^GCA
HinfI 1125 GACTC G^A[ACGT]TC
MamI 1743 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1285 CAATTG C^AATTG
MseI 218 TTAA T^TAA
MunI 1285 CAATTG C^AATTG
MvnI 379 CGCG CG^CG
NspI 1155 GCATGT [GA]CATG^[CT]
PaeR7I 640 CTCGAG C^TCGAG
PflMI 972 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1169 ACCGGT A^CCGGT
PleI 1125 GACTC GAGTC(4/5)
SexAI 1472 ACCTGGT A^CC[AT]GGT
Tru9I 218 TTAA T^TAA
Tsp509I 1286 AATT ^AATT
Van91I 972 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 640 CTCGAG C^TCGAG
XmnI 867 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 1205 TCCGGA T^CCGGA
1571 TCCGGA
AflIII 805 ACGTGT A^C[GA][CT]GT
1313 ACGTGT
AlwI 1560 GATCC GGATC(4/5)
1208 GGATC
AocI 1015 CCTGAGG CC^T[ACGT]AGG
1587 CCTCAGG
BlpI 541 GCTGAGC GC^T[ACGT]AGC
1008 GCTGAGC
Bpu1102I 541 GCTGAGC GC^T[ACGT]AGC
1008 GCTGAGC
BseAI 1205 TCCGGA T^CCGGA
1571 TCCGGA
BsmI 1548 GCATTC GAATGC(1/-1)
1679 GCATTC
BspEI 1205 TCCGGA T^CCGGA
1571 TCCGGA
BstAPI 231 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
576 GCAGGTGCTGC
BstXI 854 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1334 CCATTGGCCTGG
BstYI 212 AGATCT [GA]^GATC[CT]
1208 GGATCT
Bsu36I 1015 CCTGAGG CC^T[ACGT]AGG
1587 CCTCAGG
CelII 541 GCTGAGC GC^T[ACGT]AGC
1008 GCTGAGC
Eco81I 1015 CCTGAGG CC^T[ACGT]AGG
1587 CCTCAGG
HgaI 1405 GACGC GACGC(5/10)
743 GCGTC
MboII 1035 GAAGA GAAGA(8/7)
1226 TCTTC
MflI 212 AGATCT [GA]^GATC[CT]
1208 GGATCT
MroI 1205 TCCGGA T^CCGGA
1571 TCCGGA
MslI 1482 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1771 CACCCCAATG
PvuII 562 CAGCTG CAG^CTG
943 CAGCTG
SmlI 550 CTTGAG C^T[CT][GA]AG
640 CTCGAG
StuI 665 AGGCCT AGG^CCT
869 AGGCCT
XcmI 851 CCACCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1553 CCAAGGTGATCCTGG
XhoII 212 AGATCT [GA]^GATC[CT]
1208 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI