(Circular) (Six-base) MAPSORT of: pFN21AE0342.seq Check: 8174 from: 1 to: 6580 pFN21AE0342 6580 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2572 2854 2572 Size: 282 6298 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 4390 278 Size: 53 83 186 936 735 2119 2468 Fragments arranged by size: 2468 2119 936 735 186 83 53 AccI GT'mk_AC Cuts at: 1937 3923 1937 Size: 1986 4594 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2245 3059 4643 6027 6295 1632 Size: 613 814 1584 1384 268 1917 Fragments arranged by size: 1917 1584 1384 814 613 268 AfeI AGC'GCT Cuts at: 2359 2359 Size: 6580 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2810 3318 5479 6456 1203 Size: 1607 508 2161 977 1327 Fragments arranged by size: 2161 1607 1327 977 508 AgeI A'CCGG_T Cuts at: 3174 5411 3174 Size: 2237 4343 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 3491 1166 Size: 2325 4255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2613 1184 Size: 1429 5151 AlwNI CAG_nnn'CTG Cuts at: 2587 3013 3401 4323 5895 2587 Size: 426 388 922 1572 3272 Fragments arranged by size: 3272 1572 922 426 388 ApaI G_GGCC'C Cuts at: 2033 3289 3804 2033 Size: 1256 515 4809 Fragments arranged by size: 4809 1256 515 ApaLI G'TGCA_C Cuts at: 2840 4637 5793 2840 Size: 1797 1156 3627 Fragments arranged by size: 3627 1797 1156 ApoI r'AATT_y Cuts at: 1493 3889 4115 4267 6376 1493 Size: 2396 226 152 2109 1697 Fragments arranged by size: 2396 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2645 3414 3624 3905 1870 Size: 73 231 471 769 210 281 4545 Fragments arranged by size: 4545 769 471 281 231 210 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2396 3329 3335 3481 Size: 325 217 627 308 301 933 6 146 Cuts at: 3481 3901 5356 618 Size: 420 1455 1842 Fragments arranged by size: 1842 1455 933 627 420 325 308 301 217 146 6 BanII G_rGCy'C Cuts at: 1391 2033 2181 3020 3289 3664 3804 3899 1391 Size: 642 148 839 269 375 140 95 4072 Fragments arranged by size: 4072 839 642 375 269 148 140 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3047 928 Size: 971 1148 4461 Fragments arranged by size: 4461 1148 971 BbvCI CC'TCA_GC Cuts at: 2169 2169 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 1548 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3144 5191 136 Size: 107 122 71 631 605 1472 2047 1525 Fragments arranged by size: 2047 1525 1472 631 605 122 107 71 BglII A'GATC_T Cuts at: 2217 6575 2217 Size: 4358 2222 BlpI GC'TnA_GC Cuts at: 2547 3014 3997 2547 Size: 467 983 5130 Fragments arranged by size: 5130 983 467 Bme1580I G_kGCm'C Cuts at: 1790 2033 2074 2100 2401 2541 2844 3289 3486 Size: 243 41 26 301 140 303 445 197 Cuts at: 3486 3804 4641 5797 1790 Size: 318 837 1156 2573 Fragments arranged by size: 2573 1156 837 445 318 303 301 243 197 140 41 26 BmgBI CAC'GTC Cuts at: 1318 2515 2811 3319 1318 Size: 1197 296 508 4579 Fragments arranged by size: 4579 1197 508 296 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3292 3870 4311 Size: 416 53 83 399 24 1875 578 441 Cuts at: 4311 5269 442 Size: 958 1753 Fragments arranged by size: 1875 1753 958 578 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2249 2591 2705 3176 3798 5240 1239 Size: 902 108 342 114 471 622 1442 2579 Fragments arranged by size: 2579 1442 902 622 471 342 114 108 Bpu10I CC'TnA_GC Cuts at: 2075 2169 2075 Size: 94 6486 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 4705 5570 5868 2576 Size: 2129 865 298 3288 Fragments arranged by size: 3288 2129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4371 493 Size: 711 3167 2702 Fragments arranged by size: 3167 2702 711 BsaBI GATnn'nnATC Cuts at: 3752 6574 3752 Size: 2822 3758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 2748 3411 4387 Size: 53 83 186 936 735 480 663 976 Cuts at: 4387 4769 5404 275 Size: 382 635 1451 Fragments arranged by size: 1451 976 936 735 663 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2892 3174 3210 3576 5013 5411 5685 5832 6243 Size: 282 36 366 1437 398 274 147 411 Cuts at: 6243 2892 Size: 3229 Fragments arranged by size: 3229 1437 411 398 366 282 274 147 36 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6355 Fragments arranged by size: 6355 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2479 3038 3128 1186 Size: 952 341 559 90 4638 Fragments arranged by size: 4638 952 559 341 90 BseYI C'CCAG_C Cuts at: 2144 2706 3167 3510 5783 6279 2144 Size: 562 461 343 2273 496 2445 Fragments arranged by size: 2445 2273 562 496 461 343 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6007 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3698 4030 4791 5819 1540 Size: 275 186 1697 332 761 1028 2301 Fragments arranged by size: 2301 1697 1028 761 332 275 186 BsiHKAI G_wGCw'C Cuts at: 2618 2844 3264 3777 4641 4726 5797 2618 Size: 226 420 513 864 85 1071 3401 Fragments arranged by size: 3401 1071 864 513 420 226 85 BsmI GAATG_Cn' Cuts at: 3553 3684 4091 4184 3553 Size: 131 407 93 5949 Fragments arranged by size: 5949 407 131 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2095 2918 3616 2095 Size: 823 698 5059 Fragments arranged by size: 5059 823 698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2074 2100 2181 2401 2541 2618 Size: 399 243 41 26 81 220 140 77 Cuts at: 2618 2844 3020 3264 3289 3486 3664 3777 3804 Size: 226 176 244 25 197 178 113 27 Cuts at: 3804 3899 4641 4726 5797 1391 Size: 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 244 243 226 220 197 178 176 140 113 95 85 81 77 41 27 26 25 BspEI T'CCGG_A Cuts at: 3210 3576 6243 3210 Size: 366 2667 3547 Fragments arranged by size: 3547 2667 366 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2572 2854 3104 3201 3935 844 Size: 1728 282 250 97 734 3489 Fragments arranged by size: 3489 1728 734 282 250 97 BsrBI CCG'CTC Cuts at: 1394 1823 2560 4467 1394 Size: 429 737 1907 3507 Fragments arranged by size: 3507 1907 737 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2988 5075 5249 65 Size: 1183 1740 2087 174 1396 Fragments arranged by size: 2087 1740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3174 3332 3697 5224 5411 1700 Size: 110 1364 158 365 1527 187 2869 Fragments arranged by size: 2869 1527 1364 365 187 158 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 2502 3185 3518 4640 5652 2502 Size: 683 333 1122 1012 3430 Fragments arranged by size: 3430 1122 1012 683 333 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 2587 794 Size: 1448 345 4787 Fragments arranged by size: 4787 1448 345 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 2518 1184 Size: 1334 5246 BstXI CCAn_nnnn'nTGG Cuts at: 2866 3346 2866 Size: 480 6100 BstYI r'GATC_y Cuts at: 1972 2217 3213 3910 4277 4662 4679 6131 6217 Size: 245 996 697 367 385 17 1452 86 Cuts at: 6217 6229 6575 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 996 697 385 367 346 245 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3021 3593 3021 Size: 572 6008 BtgI C'CryG_G Cuts at: 513 1065 2040 2454 2775 2982 3633 513 Size: 552 975 414 321 207 651 3460 Fragments arranged by size: 3460 975 651 552 414 321 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 2074 3457 3720 4096 4890 4910 2074 Size: 1383 263 376 794 20 3744 Fragments arranged by size: 3744 1383 794 376 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 2224 3884 2224 Size: 1660 4920 DraIII CAC_nnn'GTG Cuts at: 2423 3520 3542 2423 Size: 1097 22 5461 Fragments arranged by size: 5461 1097 22 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2457 2508 Size: 54 1425 50 275 18 223 404 51 Cuts at: 2508 2985 3522 3570 3695 4027 4916 6545 8 Size: 477 537 48 125 332 889 1629 43 Fragments arranged by size: 1629 1425 889 537 477 404 332 275 223 125 54 51 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3695 4027 1537 Size: 275 1883 332 4090 Fragments arranged by size: 4090 1883 332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4510 1353 Size: 3157 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2245 2249 2591 2705 3059 3176 Size: 393 509 104 4 342 114 354 117 Cuts at: 3176 3798 4643 5240 6027 6295 1239 Size: 622 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 622 597 509 393 354 342 268 117 114 104 4 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2123 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 2029 2165 2377 3145 3190 3475 3800 3801 3833 Size: 136 212 768 45 285 325 1 32 Cuts at: 3833 3864 2029 Size: 31 4745 Fragments arranged by size: 4745 768 325 285 212 136 45 32 31 1 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FspI TGC'GCA Cuts at: 2960 5086 2960 Size: 2126 4454 FspAI rTGC'GCAy Cuts at: 2960 2960 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2145 2278 2361 5727 1678 Size: 467 133 83 3366 2531 Fragments arranged by size: 3366 2531 467 133 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2704 2736 5221 5253 5295 Size: 32 135 32 1132 32 2485 32 42 Cuts at: 5295 5327 1373 Size: 32 2626 Fragments arranged by size: 2626 2485 1132 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 3290 4179 3290 Size: 889 5691 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5694 5878 1305 Size: 4389 184 2007 Fragments arranged by size: 4389 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 2459 2987 3524 3572 Size: 54 1425 343 223 404 528 537 48 Cuts at: 3572 10 Size: 3018 Fragments arranged by size: 3018 1425 537 528 404 343 223 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3780 4538 4897 5056 518 Size: 648 2325 289 758 359 159 2042 Fragments arranged by size: 2325 2042 758 648 359 289 159 MspA1I CmG'CkG Cuts at: 1606 2569 2683 2950 3996 4673 5821 6066 1606 Size: 963 114 267 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 963 677 267 245 114 NcoI C'CATG_G Cuts at: 513 1065 2040 2454 2982 3633 513 Size: 552 975 414 528 651 3460 Fragments arranged by size: 3460 975 651 552 528 414 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3164 3938 5483 1132 Size: 97 1935 774 1545 2229 Fragments arranged by size: 2229 1935 1545 774 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 5479 5479 Size: 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 2983 1108 Size: 1875 4705 PfoI T'CCnGG_A Cuts at: 2880 3276 3528 2880 Size: 396 252 5932 Fragments arranged by size: 5932 396 252 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3190 3475 3833 3864 3190 Size: 285 358 31 5906 Fragments arranged by size: 5906 358 285 31 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PspOMI G'GGCC_C Cuts at: 2029 3285 3800 2029 Size: 1256 515 4809 Fragments arranged by size: 4809 1256 515 PspXI vC'TCGA_Gb Cuts at: 2645 2645 Size: 6580 PstI C_TGCA'G Cuts at: 830 2107 2238 2301 2365 2592 3659 3932 830 Size: 1277 131 63 64 227 1067 273 3478 Fragments arranged by size: 3478 1277 1067 273 227 131 64 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2569 2950 2569 Size: 381 6199 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SexAI A'CCwGG_T Cuts at: 3477 3477 Size: 6580 SfcI C'TryA_G Cuts at: 826 1045 2103 2154 2182 2234 2297 2361 2588 Size: 219 1058 51 28 52 63 64 227 Cuts at: 2588 3655 3928 5063 5744 5935 826 Size: 1067 273 1135 681 191 1471 Fragments arranged by size: 1471 1135 1067 1058 681 273 227 219 191 64 63 52 51 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 2176 3416 3626 3907 1872 Size: 304 1240 210 281 4545 Fragments arranged by size: 4545 1240 304 281 210 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 2645 4684 5585 5847 820 Size: 197 926 612 90 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 612 262 197 90 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SrfI GCCC'GGGC Cuts at: 2176 2176 Size: 6580 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 2672 2876 1517 Size: 1155 204 5221 Fragments arranged by size: 5221 1155 204 StyI C'CwwG_G Cuts at: 513 1065 2040 2442 2454 2601 2982 3558 3633 Size: 552 975 402 12 147 381 576 75 Cuts at: 3633 4019 513 Size: 386 3074 Fragments arranged by size: 3074 975 576 552 402 386 381 147 75 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4607 4624 4777 4962 5407 325 Size: 1255 3027 17 153 185 445 1498 Fragments arranged by size: 3027 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 4826 6538 Size: 274 80 33 51 1443 819 2030 1712 Cuts at: 6538 96 Size: 138 Fragments arranged by size: 2030 1712 1443 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6540 6572 6540 Size: 32 6548 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 3565 2863 Size: 702 5878 XhoI C'TCGA_G Cuts at: 1943 2645 1943 Size: 702 5878 XmaI C'CCGG_G Cuts at: 1870 2174 3414 3624 3905 1870 Size: 304 1240 210 281 4545 Fragments arranged by size: 4545 1240 304 281 210 XmnI GAAnn'nnTTC Cuts at: 2876 4709 2876 Size: 1833 4747 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 4388 276 Size: 53 83 186 936 735 2119 2468 Fragments arranged by size: 2468 2119 936 735 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SwaI