Restriction map for
pFN21AE0342
(Linear) (Six-base) MAPSORT of: KIEE0342.seq Check: 412 from: 1 to: 1875
KIEE0342.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 567 849 1875
Size: 567 282 1026
Fragments arranged by size:
1026 567 282
AatII G_ACGT'C
Cuts at: 0 266 1875
Size: 266 1609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1054 1875
Size: 240 814 821
Fragments arranged by size:
821 814 240
AfeI AGC'GCT
Cuts at: 0 354 1875
Size: 354 1521
AflIII A'CryG_T
Cuts at: 0 805 1313 1875
Size: 805 508 562
Fragments arranged by size:
805 562 508
AgeI A'CCGG_T
Cuts at: 0 1169 1875
Size: 1169 706
AleI CACnn'nnGTG
Cuts at: 0 1486 1875
Size: 1486 389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 608 1875
Size: 608 1267
AlwNI CAG_nnn'CTG
Cuts at: 0 582 1008 1396 1875
Size: 582 426 388 479
Fragments arranged by size:
582 479 426 388
ApaI G_GGCC'C
Cuts at: 0 28 1284 1799 1875
Size: 28 1256 515 76
Fragments arranged by size:
1256 515 76 28
ApaLI G'TGCA_C
Cuts at: 0 835 1875
Size: 835 1040
AvaI C'yCGr_G
Cuts at: 0 169 640 1409 1619 1875
Size: 169 471 769 210 256
Fragments arranged by size:
769 471 256 210 169
BanI G'GyrC_C
Cuts at: 0 90 391 1324 1330 1476 1875
Size: 90 301 933 6 146 399
Fragments arranged by size:
933 399 301 146 90 6
BanII G_rGCy'C
Cuts at: 0 28 176 1015 1284 1659 1799 1875
Size: 28 148 839 269 375 140 76
Fragments arranged by size:
839 375 269 148 140 76 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1042 1875
Size: 1042 833
BbvCI CC'TCA_GC
Cuts at: 0 164 1875
Size: 164 1711
BglI GCCn_nnn'nGGC
Cuts at: 0 1139 1875
Size: 1139 736
BglII A'GATC_T
Cuts at: 0 212 1875
Size: 212 1663
BlpI GC'TnA_GC
Cuts at: 0 542 1009 1875
Size: 542 467 866
Fragments arranged by size:
866 542 467
Bme1580I G_kGCm'C
Cuts at: 0 28 69 95 396 536 839 1284 1481
Size: 28 41 26 301 140 303 445 197
Cuts at: 1481 1799 1875
Size: 318 76
Fragments arranged by size:
445 318 303 301 197 140 76 41
28 26
BmgBI CAC'GTC
Cuts at: 0 510 806 1314 1875
Size: 510 296 508 561
Fragments arranged by size:
561 510 508 296
BmrI ACTGGGnnnn_n'
Cuts at: 0 1287 1865 1875
Size: 1287 578 10
Fragments arranged by size:
1287 578 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 244 586 700 1171 1793 1875
Size: 136 108 342 114 471 622 82
Fragments arranged by size:
622 471 342 136 114 108 82
Bpu10I CC'TnA_GC
Cuts at: 0 70 164 1875
Size: 70 94 1711
Fragments arranged by size:
1711 94 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1875
Size: 571 1304
BsaBI GATnn'nnATC
Cuts at: 0 1747 1875
Size: 1747 128
BsaHI Gr'CG_yC
Cuts at: 0 263 743 1406 1875
Size: 263 480 663 469
Fragments arranged by size:
663 480 469 263
BsaWI w'CCGG_w
Cuts at: 0 887 1169 1205 1571 1875
Size: 887 282 36 366 304
Fragments arranged by size:
887 366 304 282 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 474 1033 1123 1875
Size: 133 341 559 90 752
Fragments arranged by size:
752 559 341 133 90
BseYI C'CCAG_C
Cuts at: 0 139 701 1162 1505 1875
Size: 139 562 461 343 370
Fragments arranged by size:
562 461 370 343 139
BsiEI CG_ry'CG
Cuts at: 0 1693 1875
Size: 1693 182
BsiHKAI G_wGCw'C
Cuts at: 0 613 839 1259 1772 1875
Size: 613 226 420 513 103
Fragments arranged by size:
613 513 420 226 103
BsmI GAATG_Cn'
Cuts at: 0 1548 1679 1875
Size: 1548 131 196
Fragments arranged by size:
1548 196 131
BsmBI CGTCTCn'nnnn_
Cuts at: 0 90 913 1611 1875
Size: 90 823 698 264
Fragments arranged by size:
823 698 264 90
Bsp1286I G_dGCh'C
Cuts at: 0 28 69 95 176 396 536 613 839
Size: 28 41 26 81 220 140 77 226
Cuts at: 839 1015 1259 1284 1481 1659 1772 1799 1875
Size: 176 244 25 197 178 113 27 76
Fragments arranged by size:
244 226 220 197 178 176 140 113
81 77 76 41 28 27 26 25
BspEI T'CCGG_A
Cuts at: 0 1205 1571 1875
Size: 1205 366 304
Fragments arranged by size:
1205 366 304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 567 849 1099 1196 1875
Size: 567 282 250 97 679
Fragments arranged by size:
679 567 282 250 97
BsrBI CCG'CTC
Cuts at: 0 555 1875
Size: 555 1320
BsrDI GCAATG_nn'
Cuts at: 0 983 1875
Size: 983 892
BsrFI r'CCGG_y
Cuts at: 0 1169 1327 1692 1875
Size: 1169 158 365 183
Fragments arranged by size:
1169 365 183 158
BssSI C'ACGA_G
Cuts at: 0 497 1180 1513 1875
Size: 497 683 333 362
Fragments arranged by size:
683 497 362 333
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 582 1875
Size: 237 345 1293
Fragments arranged by size:
1293 345 237
BstEII G'GTnAC_C
Cuts at: 0 513 1875
Size: 513 1362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 861 1341 1875
Size: 861 480 534
Fragments arranged by size:
861 534 480
BstYI r'GATC_y
Cuts at: 0 212 1208 1875
Size: 212 996 667
Fragments arranged by size:
996 667 212
Bsu36I CC'TnA_GG
Cuts at: 0 1016 1588 1875
Size: 1016 572 287
Fragments arranged by size:
1016 572 287
BtgI C'CryG_G
Cuts at: 0 35 449 770 977 1628 1875
Size: 35 414 321 207 651 247
Fragments arranged by size:
651 414 321 247 207 35
BtsI GCAGTG_nn'
Cuts at: 0 69 1452 1715 1875
Size: 69 1383 263 160
Fragments arranged by size:
1383 263 160 69
DraI TTT'AAA
Cuts at: 0 219 1875
Size: 219 1656
DraIII CAC_nnn'GTG
Cuts at: 0 418 1515 1537 1875
Size: 418 1097 22 338
Fragments arranged by size:
1097 418 338 22
EaeI y'GGCC_r
Cuts at: 0 48 452 503 980 1517 1565 1690 1875
Size: 48 404 51 477 537 48 125 185
Fragments arranged by size:
537 477 404 185 125 51 48 48
EagI C'GGCC_G
Cuts at: 0 1690 1875
Size: 1690 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 240 244 586 700 1054 1171 1793
Size: 136 104 4 342 114 354 117 622
Cuts at: 1793 1875
Size: 82
Fragments arranged by size:
622 354 342 136 117 114 104 82
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1875
Size: 118 1757
EcoO109I rG'GnC_Cy
Cuts at: 0 24 160 372 1140 1185 1470 1795 1796
Size: 24 136 212 768 45 285 325 1
Cuts at: 1796 1828 1859 1875
Size: 32 31 16
Fragments arranged by size:
768 325 285 212 136 45 32 31
24 16 1
FspI TGC'GCA
Cuts at: 0 955 1875
Size: 955 920
FspAI rTGC'GCAy
Cuts at: 0 955 1875
Size: 955 920
HaeII r_GCGC'y
Cuts at: 0 140 273 356 1875
Size: 140 133 83 1519
Fragments arranged by size:
1519 140 133 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 699 731 1875
Size: 699 32 1144
Fragments arranged by size:
1144 699 32
MfeI C'AATT_G
Cuts at: 0 1285 1875
Size: 1285 590
MscI TGG'CCA
Cuts at: 0 50 454 982 1519 1567 1875
Size: 50 404 528 537 48 308
Fragments arranged by size:
537 528 404 308 50 48
MslI CAynn'nnrTG
Cuts at: 0 1486 1775 1875
Size: 1486 289 100
Fragments arranged by size:
1486 289 100
MspA1I CmG'CkG
Cuts at: 0 564 678 945 1875
Size: 564 114 267 930
Fragments arranged by size:
930 564 267 114
NcoI C'CATG_G
Cuts at: 0 35 449 977 1628 1875
Size: 35 414 528 651 247
Fragments arranged by size:
651 528 414 247 35
NspI r_CATG'y
Cuts at: 0 1159 1875
Size: 1159 716
PflMI CCAn_nnn'nTGG
Cuts at: 0 978 1875
Size: 978 897
PfoI T'CCnGG_A
Cuts at: 0 875 1271 1523 1875
Size: 875 396 252 352
Fragments arranged by size:
875 396 352 252
PpuMI rG'GwC_Cy
Cuts at: 0 1185 1470 1828 1859 1875
Size: 1185 285 358 31 16
Fragments arranged by size:
1185 358 285 31 16
PspOMI G'GGCC_C
Cuts at: 0 24 1280 1795 1875
Size: 24 1256 515 80
Fragments arranged by size:
1256 515 80 24
PspXI vC'TCGA_Gb
Cuts at: 0 640 1875
Size: 640 1235
PstI C_TGCA'G
Cuts at: 0 102 233 296 360 587 1654 1875
Size: 102 131 63 64 227 1067 221
Fragments arranged by size:
1067 227 221 131 102 64 63
PvuII CAG'CTG
Cuts at: 0 564 945 1875
Size: 564 381 930
Fragments arranged by size:
930 564 381
SexAI A'CCwGG_T
Cuts at: 0 1472 1875
Size: 1472 403
SfcI C'TryA_G
Cuts at: 0 98 149 177 229 292 356 583 1650
Size: 98 51 28 52 63 64 227 1067
Cuts at: 1650 1875
Size: 225
Fragments arranged by size:
1067 227 225 98 64 63 52 51
28
SmaI CCC'GGG
Cuts at: 0 171 1411 1621 1875
Size: 171 1240 210 254
Fragments arranged by size:
1240 254 210 171
SmlI C'TyrA_G
Cuts at: 0 550 640 1875
Size: 550 90 1235
Fragments arranged by size:
1235 550 90
SrfI GCCC'GGGC
Cuts at: 0 171 1875
Size: 171 1704
StuI AGG'CCT
Cuts at: 0 667 871 1875
Size: 667 204 1004
Fragments arranged by size:
1004 667 204
StyI C'CwwG_G
Cuts at: 0 35 437 449 596 977 1553 1628 1875
Size: 35 402 12 147 381 576 75 247
Fragments arranged by size:
576 402 381 247 147 75 35 12
TatI w'GTAC_w
Cuts at: 0 791 1875
Size: 791 1084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 1560 1875
Size: 858 702 315
Fragments arranged by size:
858 702 315
XhoI C'TCGA_G
Cuts at: 0 640 1875
Size: 640 1235
XmaI C'CCGG_G
Cuts at: 0 169 1409 1619 1875
Size: 169 1240 210 256
Fragments arranged by size:
1240 256 210 169
XmnI GAAnn'nnTTC
Cuts at: 0 871 1875
Size: 871 1004
ZraI GAC'GTC
Cuts at: 0 264 1875
Size: 264 1611
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII EaeI EagI Eco57MI EcoNI EcoO109I FspI
FspAI HaeII Hin4I MfeI MscI MslI MspA1I NcoI
NspI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SexAI SfcI SmaI SmlI SrfI StuI StyI TatI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BmtI BplI BsaI BsaAI BsaXI BsgI
BsiWI BspHI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
CspCI DrdI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TaqII TstI Tth111I XbaI