(Circular) (Six-base) MAPSORT of: pFN21AE0217.seq Check: 9675 from: 1 to: 5221 pFN21AE0217 5221 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3031 278 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2184 2564 1937 Size: 247 380 4594 Fragments arranged by size: 4594 380 247 Acc65I G'GTAC_C Cuts at: 2542 2542 Size: 5221 AclI AA'CG_TT Cuts at: 1563 2462 3348 3721 1563 Size: 899 886 373 3063 Fragments arranged by size: 3063 899 886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3284 4668 4936 1632 Size: 1652 1384 268 1917 Fragments arranged by size: 1917 1652 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5024 AflIII A'CryG_T Cuts at: 1203 2094 4120 5097 1203 Size: 891 2026 977 1327 Fragments arranged by size: 2026 1327 977 891 AgeI A'CCGG_T Cuts at: 4052 4052 Size: 5221 AhdI GACnn_n'nnGTC Cuts at: 3950 3950 Size: 5221 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5221 AlwNI CAG_nnn'CTG Cuts at: 2964 4536 2964 Size: 1572 3649 ApaLI G'TGCA_C Cuts at: 3278 4434 3278 Size: 1156 4065 ApoI r'AATT_y Cuts at: 1493 2299 2385 2530 2756 2908 5017 1493 Size: 806 86 145 226 152 2109 1697 Fragments arranged by size: 2109 1697 806 226 152 145 86 AseI AT'TA_AT Cuts at: 160 1032 3775 160 Size: 872 2743 1606 Fragments arranged by size: 2743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 AvaI C'yCGr_G Cuts at: 1870 1943 2546 1870 Size: 73 603 4545 Fragments arranged by size: 4545 603 73 BamHI G'GATC_C Cuts at: 2551 2918 2551 Size: 367 4854 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2542 3997 618 Size: 325 217 627 755 1455 1842 Fragments arranged by size: 1842 1455 755 627 325 217 BanII G_rGCy'C Cuts at: 1391 2078 2540 1391 Size: 687 462 4072 Fragments arranged by size: 4072 687 462 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 928 Size: 971 342 3908 Fragments arranged by size: 3908 971 342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2012 2046 3412 3446 1903 Size: 34 75 34 1366 34 3678 Fragments arranged by size: 3678 1366 75 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3113 4323 3113 Size: 1210 4011 BclI T'GATC_A Cuts at: 1548 1548 Size: 5221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 3832 136 Size: 107 122 71 631 605 580 1580 1525 Fragments arranged by size: 1580 1525 631 605 580 122 107 71 BglII A'GATC_T Cuts at: 5216 5216 Size: 5221 BlpI GC'TnA_GC Cuts at: 2638 2638 Size: 5221 Bme1580I G_kGCm'C Cuts at: 1790 2133 3282 4438 1790 Size: 343 1149 1156 2573 Fragments arranged by size: 2573 1156 1149 343 BmgBI CAC'GTC Cuts at: 1318 2095 1318 Size: 777 4444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3910 442 Size: 416 53 83 399 24 1535 958 1753 Fragments arranged by size: 1753 1535 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2064 2486 3881 1239 Size: 825 422 1395 2579 Fragments arranged by size: 2579 1395 825 422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4211 4509 3346 Size: 865 298 4058 Fragments arranged by size: 4058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 2490 3884 882 Size: 617 644 347 1394 2219 Fragments arranged by size: 2219 1394 644 617 347 BsaAI yAC'GTr Cuts at: 493 1204 3012 493 Size: 711 1808 2702 Fragments arranged by size: 2702 1808 711 BsaBI GATnn'nnATC Cuts at: 2177 5215 2177 Size: 3038 2183 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3028 3410 4045 275 Size: 53 83 186 936 1495 382 635 1451 Fragments arranged by size: 1495 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3654 4052 4326 4473 4884 3654 Size: 398 274 147 411 3991 Fragments arranged by size: 3991 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4996 Fragments arranged by size: 4996 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5221 BseYI C'CCAG_C Cuts at: 4424 4920 4424 Size: 496 4725 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4648 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2671 3432 4460 1540 Size: 275 186 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 275 186 BsiHKAI G_wGCw'C Cuts at: 2078 2400 3282 3367 4438 2078 Size: 322 882 85 1071 2861 Fragments arranged by size: 2861 1071 882 322 85 BsmI GAATG_Cn' Cuts at: 2454 2732 2825 2454 Size: 278 93 4850 Fragments arranged by size: 4850 278 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2133 2400 2540 3282 3367 4438 Size: 399 288 55 267 140 742 85 1071 Cuts at: 4438 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 288 267 140 85 55 BspEI T'CCGG_A Cuts at: 4884 4884 Size: 5221 BspHI T'CATG_A Cuts at: 3110 4840 3110 Size: 1730 3491 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2576 844 Size: 1732 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3108 1394 Size: 429 1285 3507 Fragments arranged by size: 3507 1285 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3716 3890 65 Size: 1183 2468 174 1396 Fragments arranged by size: 2468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3865 4052 1700 Size: 110 2055 187 2869 Fragments arranged by size: 2869 2055 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5221 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5221 BssSI C'ACGA_G Cuts at: 3281 4293 3281 Size: 1012 4209 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5221 BstBI TT'CG_AA Cuts at: 4025 4025 Size: 5221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5221 BstYI r'GATC_y Cuts at: 1972 2551 2918 3303 3320 4772 4858 4870 5216 Size: 579 367 385 17 1452 86 12 346 Cuts at: 5216 1972 Size: 1977 Fragments arranged by size: 1977 1452 579 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 3700 Fragments arranged by size: 3700 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2043 2435 487 Size: 683 56 817 392 3273 Fragments arranged by size: 3273 817 683 392 56 BtsI GCAGTG_nn' Cuts at: 2737 3531 3551 2737 Size: 794 20 4407 Fragments arranged by size: 4407 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5221 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5186 DraI TTT'AAA Cuts at: 2061 2525 2061 Size: 464 4757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4228 809 Size: 507 2912 1802 Fragments arranged by size: 2912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2053 2668 Size: 54 1425 50 275 18 189 34 615 Cuts at: 2668 3557 5186 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 615 275 189 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 2668 1537 Size: 275 856 4090 Fragments arranged by size: 4090 856 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3151 1353 Size: 1798 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3808 4182 4328 387 Size: 1064 138 2219 374 146 1280 Fragments arranged by size: 2219 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2076 2076 Size: 5221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2486 3284 3881 4668 4936 1239 Size: 393 432 422 798 597 787 268 1524 Fragments arranged by size: 1524 798 787 597 432 422 393 268 EcoRI G'AATT_C Cuts at: 2530 2908 2530 Size: 378 4843 FspI TGC'GCA Cuts at: 3727 3727 Size: 5221 HaeII r_GCGC'y Cuts at: 1678 4368 1678 Size: 2690 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3862 3894 3936 3968 1373 Size: 32 135 32 2290 32 42 32 2626 Fragments arranged by size: 2626 2290 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2565 2811 669 Size: 988 281 627 246 3079 Fragments arranged by size: 3079 988 627 281 246 HindIII A'AGCT_T Cuts at: 748 2513 748 Size: 1765 3456 HpaI GTT'AAC Cuts at: 2811 2811 Size: 5221 KpnI G_GTAC'C Cuts at: 2546 2546 Size: 5221 MfeI C'AATT_G Cuts at: 2472 2820 2472 Size: 348 4873 MluI A'CGCG_T Cuts at: 5097 5097 Size: 5221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4335 4519 1305 Size: 3030 184 2007 Fragments arranged by size: 3030 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 10 Size: 54 1425 343 223 3176 Fragments arranged by size: 3176 1425 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 3179 3538 3697 518 Size: 648 2013 359 159 2042 Fragments arranged by size: 2042 2013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2637 3314 4462 4707 1606 Size: 1031 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3730 Fragments arranged by size: 3730 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5221 NotI GC'GGCC_GC Cuts at: 2668 2668 Size: 5221 NspI r_CATG'y Cuts at: 1132 1229 2579 4124 1132 Size: 97 1350 1545 2229 Fragments arranged by size: 2229 1545 1350 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5221 PciI A'CATG_T Cuts at: 4120 4120 Size: 5221 PflMI CCAn_nnn'nTGG Cuts at: 1108 2126 1108 Size: 1018 4203 PmeI GTTT'AAAC Cuts at: 2525 2525 Size: 5221 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 4803 4835 3290 Size: 32 1481 32 3676 Fragments arranged by size: 3676 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5221 PsiI TTA'TAA Cuts at: 2791 2791 Size: 5221 PstI C_TGCA'G Cuts at: 830 2573 830 Size: 1743 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5221 SacI G_AGCT'C Cuts at: 2078 2078 Size: 5221 SalI G'TCGA_C Cuts at: 1936 2563 1936 Size: 627 4594 SbfI CC_TGCA'GG Cuts at: 2573 2573 Size: 5221 ScaI AGT'ACT Cuts at: 3469 5181 3469 Size: 1712 3509 SfcI C'TryA_G Cuts at: 826 1045 2569 3704 4385 4576 826 Size: 219 1524 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5221 SmaI CCC'GGG Cuts at: 1872 2548 1872 Size: 676 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3325 4226 4488 820 Size: 197 926 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3012 493 Size: 2519 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5221 SphI G_CATG'C Cuts at: 1132 2579 1132 Size: 1447 3774 SspI AAT'ATT Cuts at: 5 52 3145 5 Size: 47 3093 2081 Fragments arranged by size: 3093 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5221 StyI C'CwwG_G Cuts at: 513 1065 2004 2660 513 Size: 552 939 656 3074 Fragments arranged by size: 3074 939 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2415 3248 3265 3418 3603 4048 325 Size: 1255 835 833 17 153 185 445 1498 Fragments arranged by size: 1498 1255 835 833 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 3467 5179 Size: 274 80 33 51 1443 120 1370 1712 Cuts at: 5179 96 Size: 138 Fragments arranged by size: 1712 1443 1370 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5181 5213 5181 Size: 32 5189 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5221 XbaI T'CTAG_A Cuts at: 2557 2557 Size: 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5221 XmaI C'CCGG_G Cuts at: 1870 2546 1870 Size: 676 4545 XmnI GAAnn'nnTTC Cuts at: 3350 3350 Size: 5221 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3029 276 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI