(Circular) (Six-base) MAPSORT of: pFN21AB9701.seq Check: 331 from: 1 to: 5665 pFN21AB9701 5665 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2169 2169 Size: 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 3728 5112 5380 1632 Size: 953 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1143 953 268 AfeI AGC'GCT Cuts at: 2654 2654 Size: 5665 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 2687 Size: 32 5633 AlwNI CAG_nnn'CTG Cuts at: 2161 3408 4980 2161 Size: 1247 1572 2846 Fragments arranged by size: 2846 1572 1247 ApaI G_GGCC'C Cuts at: 2123 2381 2123 Size: 258 5407 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2110 2333 2351 2932 2990 1870 Size: 73 146 21 223 18 581 58 4545 Fragments arranged by size: 4545 581 223 146 73 58 21 18 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2733 2986 4441 618 Size: 325 217 627 537 409 253 1455 1842 Fragments arranged by size: 1842 1455 627 537 409 325 253 217 BanII G_rGCy'C Cuts at: 1391 2018 2049 2123 2211 2250 2381 2555 2580 Size: 627 31 74 88 39 131 174 25 Cuts at: 2580 2909 2984 1391 Size: 329 75 4072 Fragments arranged by size: 4072 627 329 174 131 88 75 74 39 31 25 BbeI G_GCGC'C Cuts at: 2328 2737 2328 Size: 409 5256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2728 2762 3856 3890 1903 Size: 34 791 34 1094 34 3678 Fragments arranged by size: 3678 1094 791 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2559 2568 4276 Size: 107 122 71 631 605 887 9 1708 Cuts at: 4276 136 Size: 1525 Fragments arranged by size: 1708 1525 887 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2784 5660 2784 Size: 2876 2789 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2123 2381 2454 2531 3726 4882 1790 Size: 333 258 73 77 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 333 258 77 73 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2622 3396 4354 Size: 416 53 83 399 24 1205 774 958 Cuts at: 4354 442 Size: 1753 Fragments arranged by size: 1753 1205 958 774 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 4325 1239 Size: 1475 1611 2579 Fragments arranged by size: 2579 1611 1475 Bpu10I CC'TnA_GC Cuts at: 2649 2649 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 2499 2734 3472 Size: 53 83 186 936 792 174 235 738 Cuts at: 3472 3854 4489 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 792 738 635 382 235 186 174 83 53 BsaWI w'CCGG_w Cuts at: 2799 4098 4496 4770 4917 5328 2799 Size: 1299 398 274 147 411 3136 Fragments arranged by size: 3136 1299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2687 2717 1178 Size: 30 165 30 1284 30 4126 Fragments arranged by size: 4126 1284 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2947 1186 Size: 895 866 3904 Fragments arranged by size: 3904 895 866 BseYI C'CCAG_C Cuts at: 2140 2154 2178 2346 2380 2418 2455 2557 4868 Size: 14 24 168 34 38 37 102 2311 Cuts at: 4868 5364 2140 Size: 496 2441 Fragments arranged by size: 2441 2311 496 168 102 38 37 34 24 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5092 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 2342 3115 3876 4904 1540 Size: 275 186 202 139 773 761 1028 2301 Fragments arranged by size: 2301 1028 773 761 275 202 186 139 BsiHKAI G_wGCw'C Cuts at: 2018 2939 3726 3811 4882 2018 Size: 921 787 85 1071 2801 Fragments arranged by size: 2801 1071 921 787 85 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2049 2123 2211 2250 2381 2454 Size: 399 228 31 74 88 39 131 73 Cuts at: 2454 2531 2555 2580 2909 2939 2984 3726 3811 Size: 77 24 25 329 30 45 742 85 Cuts at: 3811 4882 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 329 228 131 88 85 77 74 73 45 39 31 30 25 24 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2671 3020 844 Size: 1325 502 349 3489 Fragments arranged by size: 3489 1325 502 349 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4160 4334 65 Size: 1183 2912 174 1396 Fragments arranged by size: 2912 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2876 4309 4496 1700 Size: 110 1066 1433 187 2869 Fragments arranged by size: 2869 1433 1066 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 2392 794 Size: 1598 4067 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2604 2784 2995 3362 3747 3764 5216 5302 Size: 632 180 211 367 385 17 1452 86 Cuts at: 5302 5314 5660 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 632 385 367 346 211 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2231 2289 2317 2465 2580 513 Size: 552 1166 58 28 148 115 3598 Fragments arranged by size: 3598 1166 552 148 115 58 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3975 3995 3181 Size: 794 20 4851 Fragments arranged by size: 4851 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2339 2541 Size: 54 1425 50 275 18 370 139 202 Cuts at: 2541 3112 4001 5630 8 Size: 571 889 1629 43 Fragments arranged by size: 1629 1425 889 571 370 275 202 139 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2200 2339 3112 1537 Size: 275 388 139 773 4090 Fragments arranged by size: 4090 773 388 275 139 EarI CTCTTCn'nnn_ Cuts at: 1353 3595 1353 Size: 2242 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2016 2016 Size: 5665 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 2714 3728 4325 5112 5380 1239 Size: 393 953 129 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 953 787 597 393 268 129 EcoO109I rG'GnC_Cy Cuts at: 2119 2120 2377 2119 Size: 1 257 5407 Fragments arranged by size: 5407 257 1 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 2147 2328 2473 2520 2619 2656 2737 2776 Size: 469 181 145 47 99 37 81 39 Cuts at: 2776 4812 1678 Size: 2036 2531 Fragments arranged by size: 2531 2036 469 181 145 99 81 47 39 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373 Size: 32 135 32 2734 32 42 32 2626 Fragments arranged by size: 2734 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2623 3009 3255 669 Size: 988 281 685 386 246 3079 Fragments arranged by size: 3079 988 685 386 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 2623 3255 2623 Size: 632 5033 KasI G'GCGC_C Cuts at: 2324 2733 2324 Size: 409 5256 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 4779 4963 1305 Size: 753 2721 184 2007 Fragments arranged by size: 2721 2007 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3843 Fragments arranged by size: 3843 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2843 3623 3982 4141 518 Size: 648 843 834 780 359 159 2042 Fragments arranged by size: 2042 843 834 780 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2167 2233 2291 2319 2459 2467 2533 Size: 479 82 66 58 28 140 8 66 Cuts at: 2533 2557 3081 3758 4906 5151 1606 Size: 24 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 479 245 140 82 66 66 58 28 24 8 NaeI GCC'GGC Cuts at: 2878 2878 Size: 5665 NarI GG'CG_CC Cuts at: 2325 2734 2325 Size: 409 5256 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5113 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NgoMIV G'CCGG_C Cuts at: 2876 2876 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 2339 3112 2339 Size: 773 4892 NspI r_CATG'y Cuts at: 1132 1229 2399 3023 4568 1132 Size: 97 1170 624 1545 2229 Fragments arranged by size: 2229 1545 1170 624 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2036 2068 2687 2719 3734 3766 5247 5279 2036 Size: 32 619 32 1015 32 1481 32 2422 Fragments arranged by size: 2422 1481 1015 619 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 2634 3235 2634 Size: 601 5064 PspOMI G'GGCC_C Cuts at: 2119 2377 2119 Size: 258 5407 PstI C_TGCA'G Cuts at: 830 2172 2415 2439 3017 830 Size: 1342 243 24 578 3478 Fragments arranged by size: 3478 1342 578 243 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 RsrII CG'GwC_CG Cuts at: 2235 2235 Size: 5665 SacI G_AGCT'C Cuts at: 2018 2018 Size: 5665 SacII CC_GC'GG Cuts at: 2234 2292 2320 2468 2234 Size: 58 28 148 5431 Fragments arranged by size: 5431 148 58 28 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 2168 2411 2435 3013 4148 4829 5020 Size: 219 1123 243 24 578 1135 681 191 Cuts at: 5020 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 681 578 243 219 191 24 SfoI GGC'GCC Cuts at: 2326 2735 2326 Size: 409 5256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2112 2335 2992 1872 Size: 240 223 657 4545 Fragments arranged by size: 4545 657 240 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4670 4932 820 Size: 197 926 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 2399 3023 1132 Size: 1267 624 3774 Fragments arranged by size: 3774 1267 624 SrfI GCCC'GGGC Cuts at: 2112 2112 Size: 5665 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5665 StyI C'CwwG_G Cuts at: 513 1065 2373 3104 513 Size: 552 1308 731 3074 Fragments arranged by size: 3074 1308 731 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3911 5623 96 Size: 274 80 33 51 1443 1934 1712 138 Fragments arranged by size: 1934 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2935 2967 5625 5657 2935 Size: 32 2658 32 2943 Fragments arranged by size: 2943 2658 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2110 2333 2990 1870 Size: 240 223 657 4545 Fragments arranged by size: 4545 657 240 223 XmnI GAAnn'nnTTC Cuts at: 2702 3794 2702 Size: 1092 4573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII SanDI SapI SexAI SfiI SwaI XcmI