(Circular) (Six-base) MAPSORT of: pFN21AB7748.seq Check: 851 from: 1 to: 6169 pFN21AB7748 6169 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2029 3135 3295 4232 5616 5884 1632 Size: 397 1106 160 937 1384 268 1917 Fragments arranged by size: 1917 1384 1106 937 397 268 160 AfeI AGC'GCT Cuts at: 3424 3424 Size: 6169 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 2766 5068 6045 1203 Size: 1563 2302 977 1327 Fragments arranged by size: 2302 1563 1327 977 AgeI A'CCGG_T Cuts at: 2615 5000 2615 Size: 2385 3784 AhdI GACnn_n'nnGTC Cuts at: 3148 4898 3148 Size: 1750 4419 AleI CACnn'nnGTG Cuts at: 1166 2294 2490 1166 Size: 1128 196 4845 Fragments arranged by size: 4845 1128 196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3184 3216 3184 Size: 32 6137 AlwNI CAG_nnn'CTG Cuts at: 2052 2338 3912 5484 2052 Size: 286 1574 1572 2737 Fragments arranged by size: 2737 1574 1572 286 ApaLI G'TGCA_C Cuts at: 2636 4226 5382 2636 Size: 1590 1156 3423 Fragments arranged by size: 3423 1590 1156 ApoI r'AATT_y Cuts at: 1493 2528 2558 2740 3478 3704 3856 5965 1493 Size: 1035 30 182 738 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 738 226 182 152 30 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2430 2458 3437 3494 1870 Size: 73 487 28 979 57 4545 Fragments arranged by size: 4545 979 487 73 57 28 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 2438 3242 3380 3490 Size: 325 217 627 515 136 804 138 110 Cuts at: 3490 4945 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 804 627 515 325 217 138 136 110 BanII G_rGCy'C Cuts at: 1391 2237 3070 3104 3488 1391 Size: 846 833 34 384 4072 Fragments arranged by size: 4072 846 833 384 34 BbeI G_GCGC'C Cuts at: 2306 2442 3246 3384 2306 Size: 136 804 138 5091 Fragments arranged by size: 5091 804 138 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2187 2221 2687 2721 4360 4394 1903 Size: 34 250 34 466 34 1639 34 3678 Fragments arranged by size: 3678 1639 466 250 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 2088 3372 Size: 107 122 71 631 605 349 67 1284 Cuts at: 3372 4780 136 Size: 1408 1525 Fragments arranged by size: 1525 1408 1284 631 605 349 122 107 71 67 BglII A'GATC_T Cuts at: 2596 6164 2596 Size: 3568 2601 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2640 4230 5386 1790 Size: 850 1590 1156 2573 Fragments arranged by size: 2573 1590 1156 850 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2288 3900 4858 Size: 416 53 83 399 24 871 1612 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1753 1612 958 871 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2497 2897 4829 1239 Size: 1258 400 1932 2579 Fragments arranged by size: 2579 1932 1258 400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4294 5159 5457 4294 Size: 865 298 5006 Fragments arranged by size: 5006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4832 882 Size: 617 3333 2219 Fragments arranged by size: 3333 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2905 3960 493 Size: 711 1701 1055 2702 Fragments arranged by size: 2702 1701 1055 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2303 2439 3243 3381 Size: 53 83 186 936 770 136 804 138 Cuts at: 3381 3976 4358 4993 275 Size: 595 382 635 1451 Fragments arranged by size: 1451 936 804 770 635 595 382 186 138 136 83 53 BsaWI w'CCGG_w Cuts at: 2615 4602 5000 5274 5421 5832 2615 Size: 1987 398 274 147 411 2952 Fragments arranged by size: 2952 1987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 3184 3214 1178 Size: 30 165 30 1467 30 284 30 4133 Fragments arranged by size: 4133 1467 284 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 3112 1186 Size: 876 1050 4243 Fragments arranged by size: 4243 1050 876 BseYI C'CCAG_C Cuts at: 2941 3104 3167 3266 5372 5868 2941 Size: 163 63 99 2106 496 3242 Fragments arranged by size: 3242 2106 496 163 99 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 3191 1163 Size: 573 402 1053 4141 Fragments arranged by size: 4141 1053 573 402 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2079 2211 2664 3619 4380 Size: 275 186 60 18 132 453 955 761 Cuts at: 4380 5408 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 955 761 453 275 186 132 60 18 BsiHKAI G_wGCw'C Cuts at: 2640 2793 4230 4315 5386 2640 Size: 153 1437 85 1071 3423 Fragments arranged by size: 3423 1437 1071 153 85 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2640 2793 3070 3104 3488 4230 Size: 399 447 403 153 277 34 384 742 Cuts at: 4230 4315 5386 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 403 399 384 277 153 85 34 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2868 3524 844 Size: 2024 656 3489 Fragments arranged by size: 3489 2024 656 BsrBI CCG'CTC Cuts at: 1394 1823 2347 3181 4056 1394 Size: 429 524 834 875 3507 Fragments arranged by size: 3507 875 834 524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2159 2615 2663 2982 4813 5000 Size: 110 268 81 456 48 319 1831 187 Cuts at: 5000 1700 Size: 2869 Fragments arranged by size: 2869 1831 456 319 268 187 110 81 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 2842 3453 1925 Size: 917 611 4641 Fragments arranged by size: 4641 917 611 BssSI C'ACGA_G Cuts at: 4229 5241 4229 Size: 1012 5157 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6169 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2492 2296 Size: 196 5973 BstYI r'GATC_y Cuts at: 1972 2170 2273 2596 2686 3499 3866 4251 4268 Size: 198 103 323 90 813 367 385 17 Cuts at: 4268 5720 5806 5818 6164 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 813 385 367 346 323 198 103 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5617 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 2951 487 Size: 683 56 899 826 3705 Fragments arranged by size: 3705 899 826 683 56 BtsI GCAGTG_nn' Cuts at: 3268 3685 4479 4499 3268 Size: 417 794 20 4938 Fragments arranged by size: 4938 794 417 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2570 5176 809 Size: 507 1254 2606 1802 Fragments arranged by size: 2606 1802 1254 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2076 2208 Size: 54 1425 50 275 18 228 18 132 Cuts at: 2208 2268 2661 2665 3616 4505 6134 8 Size: 60 393 4 951 889 1629 43 Fragments arranged by size: 1629 1425 951 889 393 275 228 132 60 54 50 43 18 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2058 2076 2208 2661 3616 1537 Size: 275 246 18 132 453 955 4090 Fragments arranged by size: 4090 955 453 275 246 132 18 EarI CTCTTCn'nnn_ Cuts at: 1353 4099 1353 Size: 2746 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2409 2415 3001 4756 5130 5276 Size: 1064 138 820 6 586 1755 374 146 Cuts at: 5276 387 Size: 1280 Fragments arranged by size: 1755 1280 1064 820 586 374 146 138 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2497 2897 3135 3295 4232 4829 Size: 393 397 468 400 238 160 937 597 Cuts at: 4829 5616 5884 1239 Size: 787 268 1524 Fragments arranged by size: 1524 937 787 597 468 400 397 393 268 238 160 EcoO109I rG'GnC_Cy Cuts at: 2191 2202 2777 3229 2191 Size: 11 575 452 5131 Fragments arranged by size: 5131 575 452 11 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2149 3473 2149 Size: 1324 4845 FseI GG_CCGG'CC Cuts at: 2082 2667 2082 Size: 585 5584 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 2306 2442 3246 3321 3384 3426 5316 1678 Size: 628 136 804 75 63 42 1890 2531 Fragments arranged by size: 2531 1890 804 628 136 75 63 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 3134 3166 4810 Size: 32 135 32 1469 32 61 32 1644 Cuts at: 4810 4842 4884 4916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 1469 135 61 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KasI G'GCGC_C Cuts at: 2302 2438 3242 3380 2302 Size: 136 804 138 5091 Fragments arranged by size: 5091 804 138 136 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 2880 5283 5467 1305 Size: 759 816 2403 184 2007 Fragments arranged by size: 2403 2007 816 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4347 Fragments arranged by size: 4347 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2490 4127 4486 4645 518 Size: 648 1128 196 1637 359 159 2042 Fragments arranged by size: 2042 1637 1128 648 359 196 159 MspA1I CmG'CkG Cuts at: 1606 2335 2929 3285 3585 4262 5410 5655 1606 Size: 729 594 356 300 677 1148 245 2120 Fragments arranged by size: 2120 1148 729 677 594 356 300 245 NaeI GCC'GGC Cuts at: 2080 2161 2665 2080 Size: 81 504 5584 Fragments arranged by size: 5584 504 81 NarI GG'CG_CC Cuts at: 2303 2439 3243 3381 2303 Size: 136 804 138 5091 Fragments arranged by size: 5091 804 138 136 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5617 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NgoMIV G'CCGG_C Cuts at: 2078 2159 2663 2078 Size: 81 504 5584 Fragments arranged by size: 5584 504 81 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 3062 1108 Size: 1954 4215 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3184 3216 4238 4270 5751 5783 3184 Size: 32 1022 32 1481 32 3570 Fragments arranged by size: 3570 1481 1022 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2777 2777 Size: 6169 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PstI C_TGCA'G Cuts at: 830 2448 2557 2655 2865 3521 830 Size: 1618 109 98 210 656 3478 Fragments arranged by size: 3478 1618 656 210 109 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2929 2929 Size: 6169 RsrII CG'GwC_CG Cuts at: 2917 2917 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2444 2553 2651 2861 3517 4652 5333 Size: 219 1399 109 98 210 656 1135 681 Cuts at: 5333 5524 826 Size: 191 1471 Fragments arranged by size: 1471 1399 1135 681 656 219 210 191 109 98 SfoI GGC'GCC Cuts at: 2304 2440 3244 3382 2304 Size: 136 804 138 5091 Fragments arranged by size: 5091 804 138 136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 2159 1700 Size: 459 5710 SmaI CCC'GGG Cuts at: 1872 2432 3496 1872 Size: 560 1064 4545 Fragments arranged by size: 4545 1064 560 SmlI C'TyrA_G Cuts at: 820 1017 1943 4273 5174 5436 820 Size: 197 926 2330 901 262 1553 Fragments arranged by size: 2330 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 2649 2874 1517 Size: 1132 225 4812 Fragments arranged by size: 4812 1132 225 StyI C'CwwG_G Cuts at: 513 1065 2103 3006 3367 3448 3608 513 Size: 552 1038 903 361 81 160 3074 Fragments arranged by size: 3074 1038 903 552 361 160 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2934 4196 4213 4366 4551 4996 325 Size: 1255 1354 1262 17 153 185 445 1498 Fragments arranged by size: 1498 1354 1262 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4415 6127 96 Size: 274 80 33 51 1443 2438 1712 138 Fragments arranged by size: 2438 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6129 6161 6129 Size: 32 6137 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 2683 3505 2683 Size: 822 5347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2296 3455 2296 Size: 1159 5010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2430 3494 1870 Size: 560 1064 4545 Fragments arranged by size: 4545 1064 560 XmnI GAAnn'nnTTC Cuts at: 4298 4298 Size: 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI