Restriction map for
pFN21AB7327
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AspEI AspHI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrFI BsrGI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I CelII
CfoI Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT14I EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI HphI KasI MluI MluNI
MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI StuI
StyI SwaI Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 18 CGCG CG^CG
AccIII 605 TCCGGA T^CCGGA
AflIII 1469 ACATGT A^C[GA][CT]GT
AocI 2302 CCTAAGG CC^T[ACGT]AGG
Asp700I 511 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 598 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1349 GAAGAC GAAGAC(2/6)
BcnI 81 CCCGG CC^[GC]GG
BpuAI 1349 GAAGAC GAAGAC(2/6)
BsaBI 2380 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1013 GAGACC GGTCTC(1/5)
BsaWI 605 TCCGGA [AT]^CCGG[AT]
BseAI 605 TCCGGA T^CCGGA
BsgI 1318 CTGCAC GTGCAG(16/14)
BsiYI 2091 CCCCCAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 2091 CCCCCAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1013 GAGAC GTCTC(1/5)
BsmFI 961 GGGAC GGGAC(10/14)
BspEI 605 TCCGGA T^CCGGA
BspLU11I 1469 ACATGT A^CATGT
BsrBI 133 GAGCGG CCGCTC(-3/-3)
BssSI 2021 CACGAG CACGAG(-5/-1)
BstAPI 1311 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 534 TTCGAA TT^CGAA
BstUI 18 CGCG CG^CG
Bsu36I 2302 CCTAAGG CC^T[ACGT]AGG
DraI 318 TTTAAA TTT^AAA
DraII 813 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1846 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 17 CCGCGG C^C[GA][CT]GG
EaeI 83 CGGCCA [CT]^GGCC[GA]
Eco81I 2302 CCTAAGG CC^T[ACGT]AGG
EcoO109I 813 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 865 GAATTC G^AATTC
EcoRV 886 GATATC GAT^ATC
HindIII 1227 AAGCTT A^AGCTT
KspI 17 CCGCGG CCGC^GG
MamI 2380 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 605 TCCGGA T^CCGGA
MvnI 18 CGCG CG^CG
NciI 81 CCCGG CC^[GC]GG
NdeI 1312 CATATG CA^TATG
NspV 534 TTCGAA TT^CGAA
PflFI 598 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 813 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1352 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2207 CAGCTG CAG^CTG
SacII 17 CCGCGG CCGC^GG
SfuI 534 TTCGAA TT^CGAA
SpeI 1252 ACTAGT A^CTAGT
Tsp45I 1844 GTCAC ^GT[GC]AC
Tth111I 598 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 511 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 191 GGTACC G^GTACC
674 GGTACC
Asp718I 191 GGTACC G^GTACC
674 GGTACC
BamHI 1477 GGATCC G^GATCC
1989 GGATCC
BanI 191 GGTACC G^G[CT][GA]CC
674 GGTACC
BpmI 41 CTCCAG CTGGAG(16/14)
656 CTGGAG
BsmI 1817 GAATGC GAATGC(1/-1)
2430 GAATGC
BspDI 1768 ATCGAT AT^CGAT
2182 ATCGAT
ClaI 1768 ATCGAT AT^CGAT
2182 ATCGAT
FauI 16 CCCGC CCCGC(4/6)
19 GCGGG
HapII 82 CCGG C^CGG
606 CCGG
HincII 177 GTCGAC GT[CT]^[GA]AC
1517 GTCGAC
HindII 177 GTCGAC GT[CT]^[GA]AC
1517 GTCGAC
HpaII 82 CCGG C^CGG
606 CCGG
KpnI 191 GGTACC GGTAC^C
674 GGTACC
MaeII 924 ACGT A^CGT
991 ACGT
MslI 66 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1887 CATTTTGATG
MspI 82 CCGG C^CGG
606 CCGG
PstI 1678 CTGCAG CTGCA^G
2051 CTGCAG
SalI 177 GTCGAC G^TCGAC
1517 GTCGAC
SspI 409 AATATT AAT^ATT
943 AATATT
XbaI 2329 TCTAGA T^CTAGA
2388 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BbvI BclI BfaI BglII BsaJI
BseRI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FbaI Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII